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Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells

1
Department of Medical Science and Technology, Hiroshima International University, Higashi-hiroshima, Hiroshima 739-2695, Japan
2
Laboratory of Pharmaceutics, Gifu Pharmaceutical University, Gifu 501-1196, Japan
3
Division of Cell Growth and Tumor Regulation, Proteo-Science Center, Ehime University, Toon, Shitsukawa, Ehime 791-0295, Japan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2021, 26(1), 90; https://doi.org/10.3390/molecules26010090
Received: 4 December 2020 / Revised: 24 December 2020 / Accepted: 24 December 2020 / Published: 28 December 2020
Ferulic Acid (FA) is a highly abundant phenolic phytochemical which is present in plant tissues. FA has biological effects on physiological and pathological processes due to its anti-apoptotic and anti-oxidative properties, however, the detailed mechanism(s) of function is poorly understood. We have identified FA as a molecule that inhibits apoptosis induced by hydrogen peroxide (H2O2) or actinomycin D (ActD) in rat pheochromocytoma, PC12 cell. We also found that FA reduces H2O2-induced reactive oxygen species (ROS) production in PC12 cell, thereby acting as an anti-oxidant. Then, we analyzed FA-mediated signaling responses in rat pheochromocytoma, PC12 cells using antibody arrays for phosphokinase and apoptosis related proteins. This FA signaling pathway in PC12 cells includes inactivation of pro-apoptotic proteins, SMAC/Diablo and Bad. In addition, FA attenuates the cell injury by H2O2 through the inhibition of phosphorylation of the extracellular signal-regulated kinase (ERK). Importantly, we find that FA restores expression levels of brain-derived neurotrophic factor (BDNF), a key neuroprotective effector, in H2O2-treated PC12 cells. As a possible mechanism, FA increases BDNF by regulating microRNA-10b expression following H2O2 stimulation. Taken together, FA has broad biological effects as a neuroprotective modulator to regulate the expression of phosphokinases, apoptosis-related proteins and microRNAs against oxidative stress in PC12 cells. View Full-Text
Keywords: oxidative stress; ferulic acid; apoptosis; cell signaling oxidative stress; ferulic acid; apoptosis; cell signaling
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MDPI and ACS Style

Nakayama, H.; Nakahara, M.; Matsugi, E.; Soda, M.; Hattori, T.; Hara, K.; Usami, A.; Kusumoto, C.; Higashiyama, S.; Kitaichi, K. Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells. Molecules 2021, 26, 90. https://doi.org/10.3390/molecules26010090

AMA Style

Nakayama H, Nakahara M, Matsugi E, Soda M, Hattori T, Hara K, Usami A, Kusumoto C, Higashiyama S, Kitaichi K. Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells. Molecules. 2021; 26(1):90. https://doi.org/10.3390/molecules26010090

Chicago/Turabian Style

Nakayama, Hironao, Masako Nakahara, Erina Matsugi, Midori Soda, Tomoka Hattori, Koki Hara, Ayuki Usami, Chiaki Kusumoto, Shigeki Higashiyama, and Kiyoyuki Kitaichi. 2021. "Protective Effect of Ferulic Acid against Hydrogen Peroxide Induced Apoptosis in PC12 Cells" Molecules 26, no. 1: 90. https://doi.org/10.3390/molecules26010090

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