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In Situ Separation and Analysis of Lipids by Paper Spray Ionization Mass Spectrometry

Department of Chemistry, Dongguk University, Seoul 04620, Korea
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Academic Editor: Joselito P. Quirino
Molecules 2021, 26(1), 93; https://doi.org/10.3390/molecules26010093
Received: 19 November 2020 / Revised: 24 December 2020 / Accepted: 25 December 2020 / Published: 28 December 2020
(This article belongs to the Section Analytical Chemistry)
Paper spray ionization (PSI) is an extractive ambient ionization technique for mass spectrometry (MS), whereby a triangular paper tip serves as the sampling base and the electrospray tip. During PSI, analytes are extracted and transported to the edge of the paper tip by the applied spraying solvent. Analytes can be purified from a sample matrix and separated from each other by this transportation process. In this study, we investigated and utilized the analyte transportation process of PSI for the in situ separation and analysis of lipid mixtures. We found that differential transport of phosphatidylcholine (PC) and triacylglycerol (TAG), the two most abundant lipid classes in animals, occurred during PSI. We also found that the order in which these lipids moved strongly depended on how the spraying solvent was applied to the paper base. The more polar PC moved faster than the less polar TAG during PSI, when a polar solvent was slowly fed into a paper tip, whereas TAG was transported faster than PC when excess solvent was applied to the tip at once. In addition, we achieved a complete separation and detection of PC and TAG by slowly supplying a nonpolar solvent to a PSI tip. View Full-Text
Keywords: paper spray ionization; mass spectrometry; lipids; phosphatidylcholine; triacylglycerol paper spray ionization; mass spectrometry; lipids; phosphatidylcholine; triacylglycerol
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MDPI and ACS Style

Kal, Y.; Cha, S. In Situ Separation and Analysis of Lipids by Paper Spray Ionization Mass Spectrometry. Molecules 2021, 26, 93. https://doi.org/10.3390/molecules26010093

AMA Style

Kal Y, Cha S. In Situ Separation and Analysis of Lipids by Paper Spray Ionization Mass Spectrometry. Molecules. 2021; 26(1):93. https://doi.org/10.3390/molecules26010093

Chicago/Turabian Style

Kal, Youngju, and Sangwon Cha. 2021. "In Situ Separation and Analysis of Lipids by Paper Spray Ionization Mass Spectrometry" Molecules 26, no. 1: 93. https://doi.org/10.3390/molecules26010093

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