Next Article in Journal
Structure-Guided Identification of Black Cohosh (Actaea racemosa) Triterpenoids with In Vitro Activity against Multiple Myeloma
Previous Article in Journal
Methods of Granulocyte Isolation from Human Blood and Labeling with Multimodal Superparamagnetic Iron Oxide Nanoparticles
Open AccessArticle

Stevia Rebaudiana Bertoni, a Source of High-Potency Natural Sweetener—Biochemical and Genetic Characterization

1
Department of Genetics and Horticultural Plant Breeding, Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Akademicka 15 Street, 20-950 Lublin, Poland
2
Department of Vegetable Crops and Medicinal Plants, University of Life Sciences, Akademicka 15 Street, 20-950 Lublin, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Lillian Barros, Antonios Chrysargyris, Isabel C.F.R. Ferreira, Andrei Mocan and Nikos Tzortzakis
Molecules 2020, 25(4), 767; https://doi.org/10.3390/molecules25040767 (registering DOI)
Received: 1 February 2020 / Revised: 7 February 2020 / Accepted: 9 February 2020 / Published: 11 February 2020
Stevia rebaudiana is a natural sweetener herb that is increasingly used in herbal medicines in the food and cosmetics industries. Molecular methods can be combined with morphological techniques to identify stevia genotypes as a starting material to produce more reliable bioproducts. This study evaluated the level of the genetic and biochemical diversity in various stevia genotypes using HPLC (high performance liquid chromatography) analysis and random amplified polymorphic DNA (RAPD) markers. Stevia genotypes collected from different locations of the world showed clear variations at the biochemical and genetic level in Polish climate conditions. The influence of the genotypes on the content of steviol glycosides, antioxidants, phenols, flavonoids, and tannins was analyzed using phytochemical assays. Genotypes from Morocco, Poland, Egypt, and Nigeria can be defined as samples of higher quality compared to other genotypes analyzed in terms of the amount of steviol glycosides. Considering the rebaudioside A/stevioside ratio as a selection criterion, genotypes from Australia, China, India, and Pakistan should be considered to be valuable in terms of suitability for obtaining new varieties. The present results of RAPD marker analysis indicated differential banding pattern and considerable polymorphism among all ten stevia genotypes. Genotypes from Morocco, Egypt, Poland, Nigeria, China, and India, as genetically different, can be selected for further stevia breeding programs. View Full-Text
Keywords: Stevia rebaudiana Bertoni; steviol glycosides; polyphenolics; phenolic acids; tannins; flavonoid; RAPD Stevia rebaudiana Bertoni; steviol glycosides; polyphenolics; phenolic acids; tannins; flavonoid; RAPD
Show Figures

Figure 1

MDPI and ACS Style

Dyduch-Siemińska, M.; Najda, A.; Gawroński, J.; Balant, S.; Świca, K.; Żaba, A. Stevia Rebaudiana Bertoni, a Source of High-Potency Natural Sweetener—Biochemical and Genetic Characterization. Molecules 2020, 25, 767.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop