Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Structural Elucidation
2.2. Bioassays
3. Conclusions
4. Materials and Methods
4.1. General Experimental Procedures
4.2. Isolation of The Fungal Strain
4.3. Cultivation and Extraction of The Fungal Strain
4.4. Isolation of Secondary Metabolites
4.5. Pyruvate Dehydrogenase Complex (PDH) Cellular Activity
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Position | δC | δH (J in Hz) | Position | δC | δH (J in Hz) |
---|---|---|---|---|---|
1 | 165.5 | 9 | 125.8 | 7.17, s | |
2 | 99.6 | 10 | 196.5 | ||
3 | 163.8 | 11 | 87.5 | ||
4 | 105.0 | 6.81, d (2.0) | 11-OH | 7.25, s | |
5 | 166.0 | 12 | 88.8 | ||
5-OCH3 | 56.4 | 3.92, s | 13 | 24.3 | 1.51, s |
6 | 106.0 | 7.23, d (2.0) | 14 | 168.0 | |
7 | 131.5 | 14-OCH3 | 53.0 | 3.61, s | |
8 | 163.5 | 3-OH | 11.12, s |
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Lee, C.; Li, W.; Bang, S.; Lee, S.J.; Kang, N.-y.; Kim, S.; Kim, T.I.; Go, Y.; Shim, S.H. Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity. Molecules 2019, 24, 4450. https://doi.org/10.3390/molecules24244450
Lee C, Li W, Bang S, Lee SJ, Kang N-y, Kim S, Kim TI, Go Y, Shim SH. Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity. Molecules. 2019; 24(24):4450. https://doi.org/10.3390/molecules24244450
Chicago/Turabian StyleLee, Changyeol, Wei Li, Sunghee Bang, Sun Joo Lee, Nam-young Kang, Soonok Kim, Tae In Kim, Younghoon Go, and Sang Hee Shim. 2019. "Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity" Molecules 24, no. 24: 4450. https://doi.org/10.3390/molecules24244450