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Open AccessArticle

High-Throughput Crystallization Pipeline at the Crystallography Core Facility of the Institut Pasteur

1
Plate-forme de cristallographie-C2RT, Institut Pasteur, CNRS UMR-3528, 75724 Paris, France
2
Unité de Microbiologie Structurale, Institut Pasteur, CNRS UMR-3528, Université de Paris, 75724 Paris, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Silvano Geremia
Molecules 2019, 24(24), 4451; https://doi.org/10.3390/molecules24244451
Received: 30 October 2019 / Revised: 2 December 2019 / Accepted: 3 December 2019 / Published: 5 December 2019
(This article belongs to the Special Issue Frontier of Protein Crystallography)
The availability of whole-genome sequence data, made possible by significant advances in DNA sequencing technology, led to the emergence of structural genomics projects in the late 1990s. These projects not only significantly increased the number of 3D structures deposited in the Protein Data Bank in the last two decades, but also influenced present crystallographic strategies by introducing automation and high-throughput approaches in the structure-determination pipeline. Today, dedicated crystallization facilities, many of which are open to the general user community, routinely set up and track thousands of crystallization screening trials per day. Here, we review the current methods for high-throughput crystallization and procedures to obtain crystals suitable for X-ray diffraction studies, and we describe the crystallization pipeline implemented in the medium-scale crystallography platform at the Institut Pasteur (Paris) as an example. View Full-Text
Keywords: macromolecular crystallography pipeline; high-throughput crystallization screening; crystal optimization macromolecular crystallography pipeline; high-throughput crystallization screening; crystal optimization
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Weber, P.; Pissis, C.; Navaza, R.; Mechaly, A.E.; Saul, F.; Alzari, P.M.; Haouz, A. High-Throughput Crystallization Pipeline at the Crystallography Core Facility of the Institut Pasteur. Molecules 2019, 24, 4451.

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