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Open AccessArticle

Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification

College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Eastern HuaLan Avenue, Xinxiang 453003, China
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Author to whom correspondence should be addressed.
These two authors contributed equally to this work.
Academic Editors: Marco Rossi, Daniele Passeri and Francesca A. Scaramuzzo
Molecules 2019, 24(23), 4364; https://doi.org/10.3390/molecules24234364
Received: 25 September 2019 / Revised: 16 November 2019 / Accepted: 27 November 2019 / Published: 29 November 2019
The frequent emergence of secondary infection and immunosuppression after porcine circovirus type 2 (PCV2) infection highlights the need to develop sensitive detection methods. A dual-signal amplification enzyme-linked immunosorbent assay (ELISA) based on a microplate coated with gold nanoparticle layers (GNPL) and tyramide signal amplification (TSA) was established. Results confirmed that the microplates coated with GNPL have a strong binding ability to the antibody without affecting the biological activity of the antibody. The microplates coated with GNPL have strong binding ability to the antibody, and the amplification of the tyramide signal is combined to further improve the sensitivity of PCV2. The PCV2 antibody does not crossreact with other viruses, demonstrating that the method has good specificity. A dual-signal amplification strategy is developed using microplates modified with GNPL and TSA to sensitively detect PCV2. View Full-Text
Keywords: gold nanoparticle; PCV2; sensitive; amplification; tyramide gold nanoparticle; PCV2; sensitive; amplification; tyramide
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MDPI and ACS Style

Zhang, S.; Hu, B.; Xia, X.; Xu, Y.; Hang, B.; Jiang, J.; Hu, J. Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification. Molecules 2019, 24, 4364.

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