Alpinia zerumbet (Pers.): Food and Medicinal Plant with Potential In Vitro and In Vivo Anti-Cancer Activities
Abstract
:1. Introduction
2. Results
2.1. Cytotoxicity of Methanol Extracts of Fifteen Egyptian Medicinal Plants Species
2.2. Cytotoxicity of A. zerumbet Extracts/Fractions
2.3. Structure Elucidation of DK
2.4. In Vitro Cytotoxic Activity of A. zerumbet Extracts Against Ehrlich Ascities Carcinoma (EAC) Cells
2.5. Anti-Proliferative Activity of DK
2.6. Effect of A. zerumbet Extracts on Tumor Volume (TV)
2.7. Effect of A. zerumbet Extracts on Malonaldehyde (MDA) Level
2.8. Effect of A. zerumbet Extracts on Superoxide Dismutase (SOD) Activity
2.9. Effect of A. zerumbet Extracts on Catalase (CAT) Activity
3. Discussion
4. Materials and Methods
4.1. General Experimental Procedures
4.2. Plants Material
4.3. Extraction and Isolation
4.4. Detection of the Cytotoxicity of Egyptian Medicinal Plants
4.5. Determination of the Cytotoxicity of A. zerumbet Extracts
4.6. Anti-Proliferative Activity of DK
4.6.1. Cell Lines and Cells Culture
4.6.2. Proliferation Assay
4.7. In Vivo Studies
4.7.1. Tumor Cells
4.7.2. Animals
4.7.3. Mouse Xenograft Model
4.7.4. Estimation of MDA Levels in Liver Homogenate
4.7.5. Estimation of SOD Levels in Liver Homogenate
4.7.6. Estimation of CAT Levels in Liver Homogenate
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
No. | Plant Scientific Name | Vernacular Name | Part Used | % Mortality |
---|---|---|---|---|
1 | Alpinia zerumbet | Variegated ginger | Flowers | 93.33 |
2 | Anabasis setifera | Agram, Asal, Glew عجرم, عسل، جلو | Flowers | 43.33 |
3 | Ballota undulata | Ghassa, Zafra غصة، زفرة | Flowers | 50.00 |
4 | Calathea metallica | ND | Flowers | 23.33 |
5 | Caylusea hexagyna | Danaban دنبان | Flowers | 10.00 |
6 | Echinops glaberrimus | Khashir خشير | Flowers | 56.66 |
7 | E. spinosus | Qatad, Gorreih قتاد، جريح | Flowers | 86.66 |
8 | Globularia arabica | Handaqouq, zorreiqa حندوق، زُريقة | Flowers | 46.66 |
9 | Lavandula pubescens | Atan عطن | Fruits | 63.33 |
10 | Psoralea mutisii | ND | Flowers | 13.33 |
11 | P. pubescens | ND | Flowers | 6.66 |
12 | Reseda arabica | ND | Flowers | 30.00 |
13 | Salvadora persica | Araak, Siwaak, Miswaak أراك، سواك، مسواك | Flowers | 0.00 |
14 | Salvia aegyptiaca | Ra‘alah رعلة | Flowers | 63.33 |
15 | Senecio reflexun | Morrar, Umm Lonein مررار, أم لونين | Flowers | 36.66 |
Substance | Mortality % |
---|---|
Concentration 100 (µg/mL) | |
CH2Cl2 ex. | 100.00 |
MeOH-H2O (90%) ex. | 80.00 |
H2O ex. | 50.00 |
Hexane ex. | 50.00 |
Insoluble ex. | 13.33 |
F1 | 100.00 |
F2 | 30.00 |
F3 | 20.00 |
F4 | 63.33 |
F5 | 86.66 |
DK | 100.00 |
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Zahra, M.H.; Salem, T.A.R.; El-Aarag, B.; Yosri, N.; EL-Ghlban, S.; Zaki, K.; Marei, A.H.; Abd El-Wahed, A.; Saeed, A.; Khatib, A.; et al. Alpinia zerumbet (Pers.): Food and Medicinal Plant with Potential In Vitro and In Vivo Anti-Cancer Activities. Molecules 2019, 24, 2495. https://doi.org/10.3390/molecules24132495
Zahra MH, Salem TAR, El-Aarag B, Yosri N, EL-Ghlban S, Zaki K, Marei AH, Abd El-Wahed A, Saeed A, Khatib A, et al. Alpinia zerumbet (Pers.): Food and Medicinal Plant with Potential In Vitro and In Vivo Anti-Cancer Activities. Molecules. 2019; 24(13):2495. https://doi.org/10.3390/molecules24132495
Chicago/Turabian StyleZahra, Maram Hussein, Tarek A.R. Salem, Bishoy El-Aarag, Nermeen Yosri, Samah EL-Ghlban, Kholoud Zaki, Amel H. Marei, Aida Abd El-Wahed, Aamer Saeed, Alfi Khatib, and et al. 2019. "Alpinia zerumbet (Pers.): Food and Medicinal Plant with Potential In Vitro and In Vivo Anti-Cancer Activities" Molecules 24, no. 13: 2495. https://doi.org/10.3390/molecules24132495