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Molecules 2018, 23(9), 2345;

New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of Zebrafish

Department of Biological Sciences and Technology, National University of Tainan, No. 33, Sec. 2, Shu-Lin St., Tainan 70005, Taiwan
Department of Biotechnology, Chia Nan University of Pharmacy and Science, No. 60, Sec. 1, Erh-Jen Rd., Jen-Te District, Tainan 71710, Taiwan
Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan
Department of Food Science, National Quemoy University, No. 1, University Rd., Jin-Ning Township, Kinmen County 892, Taiwan
Author to whom correspondence should be addressed.
Academic Editor: Jose M. Palomo
Received: 30 August 2018 / Revised: 12 September 2018 / Accepted: 12 September 2018 / Published: 13 September 2018
(This article belongs to the Special Issue Biomolecular Catalysts)
PDF [1976 KB, uploaded 14 September 2018]


Functional bacteria that could biotransform triterpenoids may exist in the diverse microflora of fish intestines. Ganoderic acid A (GAA) is a major triterpenoid from the medicinal fungus Ganoderma lucidum. In studying the microbial biotransformation of GAA, dozens of intestinal bacteria were isolated from the excreta of zebrafish. The bacteria’s ability to catalyze GAA were determined using ultra-performance liquid chromatography analysis. One positive strain, GA A07, was selected for functional studies. GA A07 was confirmed as Bacillus sp., based on the DNA sequences of the 16S rRNA gene. The biotransformed metabolite was purified with the preparative high-performance liquid chromatography method and identified as GAA-15-O-β-glucoside, based on the mass and nuclear magnetic resonance spectral data. The present study is the first to report the glycosylation of Ganoderma triterpenoids. Moreover, 15-O-glycosylation is a new microbial biotransformation of triterpenoids, and the biotransformed metabolite, GAA-15-O-β-glucoside, is a new compound. View Full-Text
Keywords: biotransformation; Ganoderma lucidum; ganoderic acid A; Bacillus biotransformation; Ganoderma lucidum; ganoderic acid A; Bacillus

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Chang, T.-S.; Chiang, C.-M.; Wang, T.-Y.; Lee, C.-H.; Lee, Y.-W.; Wu, J.-Y. New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of Zebrafish. Molecules 2018, 23, 2345.

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