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Open AccessArticle

High-Throughput Analysis of Selected Urinary Hydroxy Polycyclic Aromatic Hydrocarbons by an Innovative Automated Solid-Phase Microextraction

Laboratorio di Igiene e Tossicologia Industriale, Azienda Ospedaliero-Universitaria Careggi, Largo P. Palagi 1, 50139 Firenze, Italy
Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi di Firenze, Largo G.A. Brambilla 3, 50139 Firenze, Italy
Giotto Biotech Srl, Via Madonna del Piano 6, 50019 Sesto Fiorentino (Firenze), Italy
Dipartimento di Scienze Mediche e Sanità Pubblica, Università di Cagliari, Cittadella Universitaria di Monserrato, SS 554 bivio Sestu, 09042 Monserrato (Cagliari), Italy
Author to whom correspondence should be addressed.
Academic Editors: Constantinos K. Zacharis and Paraskevas D. Tzanavaras
Molecules 2018, 23(8), 1869;
Received: 22 June 2018 / Revised: 16 July 2018 / Accepted: 26 July 2018 / Published: 26 July 2018
(This article belongs to the Special Issue Solid-Phase Microextraction)
High-throughput screening of samples is the strategy of choice to detect occupational exposure biomarkers, yet it requires a user-friendly apparatus that gives relatively prompt results while ensuring high degrees of selectivity, precision, accuracy and automation, particularly in the preparation process. Miniaturization has attracted much attention in analytical chemistry and has driven solvent and sample savings as easier automation, the latter thanks to the introduction on the market of the three axis autosampler. In light of the above, this contribution describes a novel user-friendly solid-phase microextraction (SPME) off- and on-line platform coupled with gas chromatography and triple quadrupole-mass spectrometry to determine urinary metabolites of polycyclic aromatic hydrocarbons 1- and 2-hydroxy-naphthalene, 9-hydroxy-phenanthrene, 1-hydroxy-pyrene, 3- and 9-hydroxy-benzoantracene, and 3-hydroxy-benzo[a]pyrene. In this new procedure, chromatography’s sensitivity is combined with the user-friendliness of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide on-fiber SPME derivatization using direct immersion sampling; moreover, specific isotope-labelled internal standards provide quantitative accuracy. The detection limits for the seven OH-PAHs ranged from 0.25 to 4.52 ng/L. Intra-(from 2.5 to 3.0%) and inter-session (from 2.4 to 3.9%) repeatability was also evaluated. This method serves to identify suitable risk-control strategies for occupational hygiene conservation programs. View Full-Text
Keywords: SPME; OH-PAHs; gas-chromatography; MTBSTFA SPME; OH-PAHs; gas-chromatography; MTBSTFA
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Dugheri, S.; Bonari, A.; Gentili, M.; Cappelli, G.; Pompilio, I.; Bossi, C.; Arcangeli, G.; Campagna, M.; Mucci, N. High-Throughput Analysis of Selected Urinary Hydroxy Polycyclic Aromatic Hydrocarbons by an Innovative Automated Solid-Phase Microextraction. Molecules 2018, 23, 1869.

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