Preliminary Quality Evaluation and Characterization of Phenolic Constituents in Cynanchi Wilfordii Radix
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Characterization
2.2. Preliminary Quality Evaluation of CWR and CAR Using TLC
3. Experimental Section
3.1. General
3.2. Materials
3.3. Extraction and Isolation
3.4. Partial Acid Hydrolysis of Compound 1
3.5. Determination of Sugar Configuration of Compound 1
3.6. Preparation of Test Solution of the Crude Drugs for HPLC and TLC
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds 1–13 are available from the authors. |
Positions | δC | δH (J in Hz) |
---|---|---|
1 | 121.3 | |
2 | 160.9 | |
3 | 103.7 | 6.69 (d, J = 2.0) |
4 | 164.9 | |
5 | 110.8 | 6.50 (dd, J = 2.0, 8.5) |
6 | 133.3 | 7.68 (d, J = 8.5) |
7 | 200.3 | |
8 | 32.1 | 2.62 (3H, s) |
Glucose-1′ | 102.1 | 5.05 (d, J = 7.5) |
2′ | 74.1 | 3.74 (m) d |
3′ | 88.2 | 3.67 (t, J = 9.0) |
4′ | 69.7 | 3.54 (t, J = 9.0) |
5′ | 78.0 a | 3.52 (m) d |
6′ | 62.4 b | 3.94 (dd, J = 1.5, 12.0) c, 3.75 (m) c,d |
Glucose-1″ | 105.3 | 4.58 (d, J = 7.5) |
2″ | 75.5 | 3.30 (m) d |
3″ | 77.9 a | 3.39 (t, J = 9.5) |
4″ | 71.6 | 3.30 (m) d |
5″ | 78.2 a | 3.35 (m) d |
6″ | 62.6 b | 3.89 (dd, J = 2.0, 11.5) c, 3.63 (m) c,d |
Products | Crude Drug | Locality | Market |
---|---|---|---|
A | Cynanchi Wilfordii Radix (CWR) | Korea | Korea |
B | Cynanchi Wilfordii Radix (CWR) | Korea | Korea |
C | Cynanchi Wilfordii Radix (CWR) | Yeongcheon | Korea |
D | Cynanchi Wilfordii Radix (CWR) | Yeongcheon | Korea |
a | Cynanchi Auriculati Radix (CAR) | Jiangsu | China |
b | Cynanchi Auriculati Radix (CAR) | Jiangsu | China |
c | Cynanchi Auriculati Radix (CAR) | Jiangsu | China |
d | Cynanchi Auriculati Radix (CAR) | Jiangsu | China |
e | Cynanchi Auriculati Radix (CAR) | Jiangsu | China |
f | Cynanchi Auriculati Radix (CAR) | Jiangsu | China |
g | Cynanchi Auriculati Radix (CAR) | Jiangsu | China |
h | Cynanchi Auriculati Radix (CAR) | Korea | Korea |
i | Cynanchi Auriculati Radix (CAR) | Korea | Korea |
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Uchikura, T.; Tanaka, H.; Sugiwaki, H.; Yoshimura, M.; Sato-Masumoto, N.; Tsujimoto, T.; Uchiyama, N.; Hakamatsuka, T.; Amakura, Y. Preliminary Quality Evaluation and Characterization of Phenolic Constituents in Cynanchi Wilfordii Radix. Molecules 2018, 23, 656. https://doi.org/10.3390/molecules23030656
Uchikura T, Tanaka H, Sugiwaki H, Yoshimura M, Sato-Masumoto N, Tsujimoto T, Uchiyama N, Hakamatsuka T, Amakura Y. Preliminary Quality Evaluation and Characterization of Phenolic Constituents in Cynanchi Wilfordii Radix. Molecules. 2018; 23(3):656. https://doi.org/10.3390/molecules23030656
Chicago/Turabian StyleUchikura, Takashi, Hiroaki Tanaka, Hidemi Sugiwaki, Morio Yoshimura, Naoko Sato-Masumoto, Takashi Tsujimoto, Nahoko Uchiyama, Takashi Hakamatsuka, and Yoshiaki Amakura. 2018. "Preliminary Quality Evaluation and Characterization of Phenolic Constituents in Cynanchi Wilfordii Radix" Molecules 23, no. 3: 656. https://doi.org/10.3390/molecules23030656