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Open AccessArticle

Simultaneous Determination of Nε-(carboxymethyl) Lysine and Nε-(carboxyethyl) Lysine in Different Sections of Antler Velvet after Various Processing Methods by UPLC-MS/MS

1
Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China
2
College of Chinese Material Medicine, Jilin Agricultural University, Changchun 130112, China
*
Author to whom correspondence should be addressed.
Academic Editors: In-Soo Yoon and Hyun-Jong Cho
Molecules 2018, 23(12), 3316; https://doi.org/10.3390/molecules23123316
Received: 13 November 2018 / Revised: 9 December 2018 / Accepted: 12 December 2018 / Published: 14 December 2018
(This article belongs to the Special Issue Method Development and Validation in Food and Pharmaceutical Analysis)
Nε-(Carboxymethyl) lysine (CML) and Nε-(carboxyethyl) advanced glycation end-products (AGEs) and are frequently used as markers of AGE formation. AGEs, such as CML and CEL, have harmful effects in the human body and have been closely linked to many diseases such as diabetes and uremia. However, details on the contents of CML and CEL after applying different antler velvet processing methods are lacking. In this research, a robust lysine (CEL) are two typical UPLC-MS/MS method has been developed for the simultaneous determination of CML and CEL in various sections of antler velvet processed with different methods. In addition, factors affecting the CML and CEL contents are discussed. The CML contents of antler velvet after freeze-drying, boiling, processing without blood, and processing with blood were 74.55–458.59, 119.44–570.69, 75.36–234.92, and 117.11–456.01 μg/g protein, respectively; the CEL contents were 0.74–12.66, 11.33–35.93, 0.00–6.75, and 0.00–23.41 μg/g protein, respectively. The different contents of CML and CEL in the different samples of antler velvet result from the different interactions of the protein and lysine at different temperatures. These data can be used to estimate the potential consumer intake of CML and CEL from antler velvet and for guiding producers on how to reduce the production of CML and CEL. View Full-Text
Keywords: advanced glycation end-products (AGEs); Nε-(carboxymethyl) lysine (CML); Nε-(carboxyethyl) lysine (CEL); antler velvet processing; UPLC-MS/MS advanced glycation end-products (AGEs); Nε-(carboxymethyl) lysine (CML); Nε-(carboxyethyl) lysine (CEL); antler velvet processing; UPLC-MS/MS
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Gong, R.-Z.; Wang, Y.-H.; Wang, Y.-F.; Chen, B.; Gao, K.; Sun, Y.-S. Simultaneous Determination of Nε-(carboxymethyl) Lysine and Nε-(carboxyethyl) Lysine in Different Sections of Antler Velvet after Various Processing Methods by UPLC-MS/MS. Molecules 2018, 23, 3316.

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