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Open AccessArticle

Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy

by Jia Zhou 1,2,†, Hao Cai 1,2,*,†, Sicong Tu 3,4,†, Yu Duan 1,2,†, Ke Pei 5, Yangyang Xu 1,2, Jing Liu 1,2, Minjie Niu 1,2, Yating Zhang 1,2, Lin Shen 1,2 and Qigang Zhou 6
1
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2
Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China
3
Medical Sciences Division, University of Oxford, Oxford OX3 7BN, UK
4
Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia
5
Institute of Pharmaceutical and Food Engineering, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China
6
School of Pharmacy, Nanjing Medical University, Nanjing 210026, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: In-Soo Yoon and Hyun-Jong Cho
Molecules 2018, 23(12), 3128; https://doi.org/10.3390/molecules23123128
Received: 1 November 2018 / Revised: 21 November 2018 / Accepted: 26 November 2018 / Published: 29 November 2018
(This article belongs to the Special Issue Method Development and Validation in Food and Pharmaceutical Analysis)
Sinisan has been widely used to treat depression. However, its pharmacologically-effective constituents are largely unknown, and the pharmacological effects and clinical efficacies of Sinisan-containing processed medicinal herbs may change. To address these important issues, we developed an ultra-high performance liquid chromatography coupled with electrospray ionization tandem quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) method coupled with multiple statistical strategies to analyze the compound profiles of Sinisan, including individual herb, herb-pair, and complicated Chinese medicinal formula. As a result, 122 different constituents from individual herb, herb-pair, and complicated Chinese medicinal formula were identified totally. Through the comparison of three progressive levels, it suggests that processing herbal medicine and/or altering medicinal formula compatibility could change herbal chemical constituents, resulting in different pharmacological effects. This is also the first report that saikosaponin h/i and saikosaponin g have been identified in Sinisan. View Full-Text
Keywords: chemical constituent profiles of Sinisan; chinese medicine processing; chinese medicinal formula compatibility chemical constituent profiles of Sinisan; chinese medicine processing; chinese medicinal formula compatibility
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MDPI and ACS Style

Zhou, J.; Cai, H.; Tu, S.; Duan, Y.; Pei, K.; Xu, Y.; Liu, J.; Niu, M.; Zhang, Y.; Shen, L.; Zhou, Q. Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy. Molecules 2018, 23, 3128.

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