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Open AccessArticle

Chromosome Doubling-Enhanced Biomass and Dihydrotanshinone I Production in Salvia miltiorrhiza, A Traditional Chinese Medicinal Plant

1
Department of Life Sciences, National University of Kaohsiung, Kaohsiung 811, Taiwan
2
Department of Horticulture, National Ilan University, Yilan 260, Taiwan
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(12), 3106; https://doi.org/10.3390/molecules23123106
Received: 6 November 2018 / Revised: 20 November 2018 / Accepted: 21 November 2018 / Published: 27 November 2018
(This article belongs to the Collection Herbal Medicine Research)
The root of Chinese sage (Salvia miltiorrhiza Bunge) was regarded as top-grade Chinese medicine two thousand years ago, according to Shen Nong Materia Medica. The aim of this study is to develop an easy and reliable means for obtaining tetraploids (4x plants) via thidiazuron-induced direct organogenesis in the presence of colchicine. The resulting 4x plants showed significantly enhanced agronomic traits, including the size of stomata, leaflet, pollen, and seed as well as shoot length, root diameter, number of leaves, and fresh weight of plant. In addition, an obvious reduction of length to width ratio was found in the 4x plants, including stomata, leaflets, pollens, seeds, and roots. The 4x ploidy state of the plants was stable as was proved by evaluation of selection indicators as well as consistent ploidy level at 10th generation plantlets and also on 4x seedlings obtained via self-pollination. The major bioactive compounds, salvianolic acid B, tanshinone I, tanshinone IIA, dihydrotanshinone I and cryptotanshinone, as well as total tanshinones were determined by high performance liquid chromatography (HPLC). The concentrations of dihydrotanshinone I and total tanshinones in the root extract of the 4x plants were significantly higher when compared with the 2x plants. This present study developed a simple and efficient system for inducing and subculture of tetrapolids which have stable ploidy level, enhanced growth characteristics as well as the content of dihydrotanshinone I in the root of S. miltiorrhiza. View Full-Text
Keywords: antimitotic agent; direct shoot formation; flow cytometry; polyploidy; salvianolic acid B; tanshinone antimitotic agent; direct shoot formation; flow cytometry; polyploidy; salvianolic acid B; tanshinone
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MDPI and ACS Style

Chen, E.G.; Tsai, K.-L.; Chung, H.-H.; Chen, J.-T. Chromosome Doubling-Enhanced Biomass and Dihydrotanshinone I Production in Salvia miltiorrhiza, A Traditional Chinese Medicinal Plant. Molecules 2018, 23, 3106.

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