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Open AccessArticle

Soluble Epoxide Hydrolase Inhibitory Activity of Components Isolated from Apios americana Medik

Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeoungeup, Jeollabuk-do 56212, Korea
College of Pharmacy, Chungnam National University, Daejeon 34134, Korea
Author to whom correspondence should be addressed.
Molecules 2017, 22(9), 1432;
Received: 13 July 2017 / Revised: 22 August 2017 / Accepted: 28 August 2017 / Published: 30 August 2017
(This article belongs to the Collection Bioactive Compounds)
A new compound 1, 5-methoxy-2,5,7,4′-tetrahydroxy-coumaronochromone, along with seven known compounds (28), were isolated from Apios americana using open column chromatography. Their structures were established based on an analysis of 1D and 2D NMR, and MS spectra. Among these, two compounds 1 and 2 showed inhibitory activity on soluble epoxide hydrolase (sEH) at a concentration below 50 μM. The respective competitive (1) and mixed (2) inhibitors were revealed to have Ki values of 21.0 ± 0.8 and 14.5 ± 1.5 μM, based on the Dixon plot. The potential inhibitor (2) was visually presented in a predicted binding pose in the receptor by molecular docking. Additionally, molecular dynamics were performed for a detailed understanding of their complex by Gromacs 4.6.5 package. View Full-Text
Keywords: Apios americana; leguminosae; sEH inhibitor; molecular simulation Apios americana; leguminosae; sEH inhibitor; molecular simulation
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MDPI and ACS Style

Kim, J.H.; Kim, H.Y.; Kang, S.Y.; Kim, Y.H.; Jin, C.H. Soluble Epoxide Hydrolase Inhibitory Activity of Components Isolated from Apios americana Medik. Molecules 2017, 22, 1432.

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