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13 pages, 637 KB

Open AccessReview

Myostatin in Obesity: A Molecular Link Between Metabolic Dysfunction and Musculotendinous Remodeling

by Leonardo Cesanelli, Petras Minderis, Andrej Fokin, Aivaras Ratkevicius, Danguole Satkunskiene and Hans Degens

Int. J. Mol. Sci. 2026, 27(2), 967; https://doi.org/10.3390/ijms27020967 (registering DOI) - 18 Jan 2026

Abstract

Obesity is increasingly recognized not only as a metabolic disorder but also as a condition marked by the structural and functional deterioration of skeletal muscle and tendon tissues. Central to this process is the dysregulation of the extracellular matrix (ECM) resulting in fibrosis [...] Read more.

Obesity is increasingly recognized not only as a metabolic disorder but also as a condition marked by the structural and functional deterioration of skeletal muscle and tendon tissues. Central to this process is the dysregulation of the extracellular matrix (ECM) resulting in fibrosis and ectopic fat accumulation, factors that contribute to impaired tissue mechanics. Myostatin (GDF-8), a member of the TGF-β superfamily, is known as a negative regulator of muscle mass. It can also mediate interaction between adipose and other tissues including muscles and tendons. In obesity, elevated myostatin levels have been reported to be associated with insulin resistance, muscle atrophy, and activation of SMAD2/3 signaling, while experimental and preclinical studies indicate that myostatin inhibition can improve glucose homeostasis and increase lean mass. Emerging evidence suggests that myostatin also plays a critical role in muscle ECM and tendon remodeling. Restoring its physiological levels may help reverse ECM disorganization and reduce tissue fragility associated with musculotendinous dysfunction. This review highlights the multifaceted role of myostatin in obesity, beyond its role in muscle catabolism, to include modulation of structural integrity, metabolism, and mechanical adaptability of the musculotendinous system. Understanding how myostatin responds to metabolic stress and affects biomechanical remodeling offers novel insights into obesity-related muscle and tendon dysfunction. Full article

(This article belongs to the Special Issue Molecular Research in Obesity and Obesity Related Disorders: 3rd Edition)

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17 pages, 900 KB

Open AccessReview

The Impact of Selenium Exposure During Pregnancy on Risk for Miscarriage: A Systematic Review

by Stavroula-Ioanna Kyriakou, Ermioni Tsarna, Nikolina Stachika, Christina Dalla, Anastasios Potiris, Sofoklis Stavros and Panagiotis Christopoulos

Int. J. Mol. Sci. 2026, 27(2), 968; https://doi.org/10.3390/ijms27020968 (registering DOI) - 18 Jan 2026

Abstract

Selenium (Se) is an antioxidant essential trace element influencing inflammatory and immune pathways. This systematic review aimed to evaluate the role of maternal Se status during pregnancy in miscarriage risk. A systematic search of PubMed and Embase up to July 2024 was conducted [...] Read more.

Selenium (Se) is an antioxidant essential trace element influencing inflammatory and immune pathways. This systematic review aimed to evaluate the role of maternal Se status during pregnancy in miscarriage risk. A systematic search of PubMed and Embase up to July 2024 was conducted to identify relevant original research studies in English. Available evidence was qualitatively synthesized and predefined sources of bias were assessed. Of 2345 studies identified, 421 full texts were assessed and 14 were included, encompassing 2309 pregnancies. Despite notable methodological limitations across several studies, current evidence indicates that maternal blood Se concentrations are lower among women who experience miscarriage compared to those with uncomplicated pregnancies. Findings regarding placental Se levels were inconsistent, but important methodological issues were noted. Environmental Se exposure was investigated in a single low-powered study, which did not demonstrate a statistically significant association. Potential interactions between Se status, co-exposure to other environmental or lifestyle factors, and effect modification remain insufficiently explored. Adequate maternal Se status during early gestation may reduce miscarriage risk by mitigating oxidative stress and ferroptosis, supporting immune regulation, and modulating thyroid autoimmunity and function. However, causal inference cannot be established due to the absence of randomized interventional evidence. Full article

(This article belongs to the Special Issue Molecular Insights: Transitioning Fertility Research from the Laboratory to Clinical Practice)

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15 pages, 613 KB

Open AccessArticle

The Effects of a Cerebellar Transcranial Direct-Current Stimulation-Based Preventive Exercise Program on Physical Function and Fall Reduction Efficacy in Community-Dwelling Older Adults

by Deone Kang and JongEun Yim

Healthcare 2026, 14(2), 241; https://doi.org/10.3390/healthcare14020241 (registering DOI) - 18 Jan 2026

Abstract

Background/Objectives: Falls are a major cause of injury in older adults, closely related to declines in muscle strength, balance control, and sensory integration. Although exercise-based fall prevention programs are well supported, evidence on combining such programs with cerebellar transcranial direct-current stimulation (c-tDCS) [...] Read more.

Background/Objectives: Falls are a major cause of injury in older adults, closely related to declines in muscle strength, balance control, and sensory integration. Although exercise-based fall prevention programs are well supported, evidence on combining such programs with cerebellar transcranial direct-current stimulation (c-tDCS) remains limited. This study investigated the effects of c-tDCS applied before a modified Otago Exercise Program (OEP) on lower-extremity strength, balance, and fall efficacy in older adults. Methods: In this randomized controlled study, twenty-six community-dwelling older adults (median age [IQR]: experimental, 74.00 [10] years; control, 71.00 [10] years) were randomly assigned to either a c-tDCS + exercise group (n = 13) or a sham + exercise group (n = 13). The intervention was administered twice weekly for four weeks. The experimental group received 15 min of c-tDCS followed by 30 min of OEP-based exercise; the control group received sham stimulation under identical conditions. The outcome measures included the Five Times Sit to Stand Test (FTSST), Timed Up and Go (TUG), Balancia-based static balance (velocity average), and Falls Efficacy Scale—Korea (FES-K). Assessments were performed pre- and post-intervention. Results: The experimental group demonstrated significantly greater improvements than the control group (p < 0.05) in the Five Times Sit to Stand Test (r = 0.44) and Timed Up and Go test (r = 0.56). No significant changes were observed in static balance or fall efficacy in either group (p > 0.05). Conclusions: The combined use of c-tDCS and an OEP-based fall prevention exercise program effectively improved lower-extremity strength and dynamic balance in older adults. However, short-term intervention did not influence static balance or fall efficacy. Further studies using longer intervention periods and larger samples are warranted to verify these findings and clarify the mechanisms underlying c-tDCS-enhanced motor performance. Full article

(This article belongs to the Section Clinical Care)

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14 pages, 377 KB

Open AccessArticle

Comparative Diagnostic Assessment of Karyotyping, Microarray, and Whole Exome Sequencing in Genetically Associated Fetal Growth Restriction

by Libing Luo, Chunchun Chen, Cindy Ka Yee Cheung, Yanyan Li, Xiaoying Dai, Ting Zeng and Ying Wang

Diagnostics 2026, 16(2), 312; https://doi.org/10.3390/diagnostics16020312 (registering DOI) - 18 Jan 2026

Abstract

Background: Fetal growth restriction (FGR) is a significant obstetric complication associated with increased perinatal morbidity and long-term developmental risks. Despite advances in prenatal diagnosis, the genetic etiology of isolated FGR remains incompletely characterized, complicating genetic counseling and clinical management. Objective: This study aimed [...] Read more.

Background: Fetal growth restriction (FGR) is a significant obstetric complication associated with increased perinatal morbidity and long-term developmental risks. Despite advances in prenatal diagnosis, the genetic etiology of isolated FGR remains incompletely characterized, complicating genetic counseling and clinical management. Objective: This study aimed to systematically evaluate the genetic causes of isolated FGR by integrating karyotyping, chromosomal microarray analysis (CMA), and trio-based whole exome sequencing (trio-WES) and to assess the incremental diagnostic yield of this sequential approach. Methods: A retrospective cohort of 153 fetuses with isolated FGR (diagnosed by ultrasound between February 2018 and July 2024) underwent karyotyping and CMA. Cases with normal results from both tests (n = 50) were subsequently analyzed by trio-WES. Results: Karyotyping identified chromosomal abnormalities in three cases (2.0%). CMA detected pathogenic/likely pathogenic copy number variations (CNVs) or uniparental disomy (UPD) in twelve cases (7.8%), including the three karyotypic abnormalities and nine additional cases (5.9% incremental yield). Trio-WES performed on 50 CMA-negative cases identified pathogenic or likely pathogenic variants in 12 cases (24%). Among these, seven cases (14% of the WES subgroup) harbored variants directly causative of FGR, including one case of UPD(6) missed by CMA alone. Additionally, trio-WES revealed seven incidental pathogenic/likely pathogenic variants not directly linked to FGR and identified one case in which FGR was attributed to maternal hyperphenylalaninemia. Conclusions: The sequential application of CMA and trio-WES significantly improves the diagnostic yield for isolated FGR. Trio-WES proved particularly valuable in detecting UPD and single-gene variants missed by CMA alone and in revealing contributory maternal genetic conditions. These findings support the integration of advanced genetic testing into the diagnostic workup for isolated FGR to enhance etiological diagnosis, facilitate comprehensive genetic counseling, and inform multidisciplinary management. Full article

(This article belongs to the Special Issue Diagnostic Techniques in Obstetrics and Gynecology: Current Practices and Future Directions)

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24 pages, 15591 KB

Open AccessArticle

Bioprospecting Honey-Derived Microorganisms for the Biological Control of Phytopathogens

by Patrícia Perina de Oliveira, Giovanna Felette de Paula, Katherine Bilsland Marchesan, Luiza Rodrigues de Souza, José Fhilipe de Miranda da Silva, João Gabriel Elston, Henrique Marques de Souza and Elizabeth Bilsland

Microorganisms 2026, 14(1), 224; https://doi.org/10.3390/microorganisms14010224 (registering DOI) - 18 Jan 2026

Abstract

Microbial biological control agents are a sustainable alternative to synthetic pesticides, yet their widespread application is limited by a lack of environmental resilience of commercial products. To address this, we exploited honey—a stringent ecological niche—as a reservoir for stress-tolerant bacteria. In this study, [...] Read more.

Microbial biological control agents are a sustainable alternative to synthetic pesticides, yet their widespread application is limited by a lack of environmental resilience of commercial products. To address this, we exploited honey—a stringent ecological niche—as a reservoir for stress-tolerant bacteria. In this study, the bioprospection utilizing five types of commercially available honeys yielded a collection of 53 bacteria and 10 fungi. All bacterial isolates were evaluated for antimicrobial activity against a laboratory-standard bacterium and yeast, and six economically relevant phytopathogenic microorganisms. Initial screening with standard laboratory organisms proved to be an efficient method to detect strains with antimicrobial potential, correlating significantly with further phytopathogen inhibition (Spearman’s r = 0.4512, p = 0.0005). Two promising strains, M2.7 and M3.18, were selected for quantitative dual-culture assays along with molecular identification using 16S rDNA and gyrA gene sequencing, classifying them as Bacillus velezensis. These strains exhibited high inhibitory effects against the pathogens (p > 0.001), often with equivalent efficacy to the commercial biocontrol strain, and also induced significant phytopathogen hyphal deformities, such as increased septation and swelling. These findings support honey as a viable source of robust biocontrol agents, offering a sustainable strategy to substitute or complement current agrochemicals. Full article

(This article belongs to the Special Issue Microbes at the Root of Solutions for Anthropocene Challenges)

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19 pages, 1571 KB

Open AccessReview

Recent Progress in Curcumin Extraction, Synthesis, and Applications: A Comprehensive Review

by Qirui Meng, Feng Xiao, Dahai Jiang, Wenxuan Jiang, Wenze Lin, Huiliang Gan, Tong Ye, Jianchun Jiang and Liming Lu

Foods 2026, 15(2), 354; https://doi.org/10.3390/foods15020354 (registering DOI) - 18 Jan 2026

Abstract

Curcumin, a natural polyphenol derived from Curcuma longa L., exhibits diverse biological activities including anti-inflammatory, anticancer, and antioxidant effects, making it a versatile candidate for food, feed, pharmaceutical, and cosmetic applications. However, its industrial application is hindered by low bioavailability, poor water solubility, [...] Read more.

Curcumin, a natural polyphenol derived from Curcuma longa L., exhibits diverse biological activities including anti-inflammatory, anticancer, and antioxidant effects, making it a versatile candidate for food, feed, pharmaceutical, and cosmetic applications. However, its industrial application is hindered by low bioavailability, poor water solubility, and high production costs. This review comprehensively summarizes the latest advances in curcumin’s physicochemical properties, production routes (phytoextraction, chemical synthesis, and microbial biosynthesis), and wide applications. Compared with existing reviews, this work emphasizes quantitative benchmarking of production methods (yield, productivity, and environmental metrics), critical evaluation of application feasibility including regulatory hurdles and clinical evidence, and actionable future directions for industrial scalability. We systematically analyze the advantages, limitations, economic and environmental trade-offs of each production route, and highlight recent innovations in bioavailability enhancement and metabolic engineering. This review aims to provide a holistic theoretical and technical framework for accelerating curcumin’s sustainable development and commercialization in high-value products. Full article

(This article belongs to the Section Food Engineering and Technology)

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12 pages, 4673 KB

Open AccessArticle

Study on the Relationship Between Exogenous Salicylic Acid-Induced Pear Resistance to Black Spot Disease and Lignin Synthesis

by Qi Yan, Weiyi Chen, Yarui Wei, Hui Zhang, Na Liu and Yuxing Zhang

Horticulturae 2026, 12(1), 104; https://doi.org/10.3390/horticulturae12010104 (registering DOI) - 18 Jan 2026

Abstract

Pear black spot disease is a serious fungal disease during pear production; salicylic acid is a core signaling molecule that regulates the expression of plant disease resistance genes. To elucidate the intrinsic association between salicylic acid-induced resistance to pear black spot disease and [...] Read more.

Pear black spot disease is a serious fungal disease during pear production; salicylic acid is a core signaling molecule that regulates the expression of plant disease resistance genes. To elucidate the intrinsic association between salicylic acid-induced resistance to pear black spot disease and lignin biosynthesis, in vitro plantlets of two pear cultivars, ‘Xinli No.7’ and ‘Xueqing’, were employed as experimental materials. After 60 h SA pretreatment, the leaves were inoculated with the pathogen Alternaria alternata. Leaf samples were harvested at 0, 8, 16, 24, and 48 h post-inoculation to determine phenylalanine ammonia-lyase activity, quantify lignin content, and analyze the transcript levels of genes involved in lignin synthesis. The results demonstrated that, relative to the untreated control group, SA treatment significantly enhanced phenylalanine ammonia-lyase activity and promoted lignin accumulation in both ‘Xinli No.7’ and ‘Xueqing’. Moreover, multiple key genes associated with lignin biosynthesis—including PbrPAL1, Pbr4CL1, PbrCOMT, PbrCCoAOMT, PbrCAD, and PbrPOD—were markedly upregulated, with their expression levels increasing by 3.5–15 fold. Transcript profiles of PbrHCT1, PbrHCT4, and PbrC3H1 exhibited cultivar-specific divergence between the two varieties. Notably, the susceptible cultivar ‘Xueqing’ displayed a distinct lag phase and attenuated response in the expression of all lignin-related genes compared with the other cultivar. This study provides reference for green prevention and sustainable development of pear. Full article

(This article belongs to the Section Plant Pathology and Disease Management (PPDM))

18 pages, 7469 KB

Open AccessArticle

Investigating Color as a Non-Destructive Indicator of Strength Loss in High Tensile Nylon 6,6 Webbings

by Nilesh Rajendran, David Eisenberg, Brady J. Clapsaddle, Girish Srinivas and Emiel DenHartog

Textiles 2026, 6(1), 13; https://doi.org/10.3390/textiles6010013 (registering DOI) - 18 Jan 2026

Abstract

High-performance nylon 6,6 webbings used in critical applications degrade under solar exposure, necessitating reliable methods to assess their residual strength non-destructively. This study investigates the feasibility of using instrumental color change as a predictive indicator for the loss of breaking strength. Four colors [...] Read more.

High-performance nylon 6,6 webbings used in critical applications degrade under solar exposure, necessitating reliable methods to assess their residual strength non-destructively. This study investigates the feasibility of using instrumental color change as a predictive indicator for the loss of breaking strength. Four colors of nylon 6,6 webbings were subjected to accelerated xenon-arc solar weathering for up to 15 days. The resulting color change was quantified using both the CIELab and CIEDE2000 formulas, and residual breaking strength was measured following ASTM D6775. A regression analysis was performed to correlate these properties. The results demonstrate that a strong predictive relationship exists, but its efficacy is highly color-dependent. Webbing with high initial chroma, namely tan (R² = 0.889) and navy (R² = 0.817), showed a strong correlation between color change and strength loss. In contrast, the models for low-chroma black and white webbings were weak and unreliable. Furthermore, the simpler CIELab (ΔE*_ab) formula provided slightly more accurate predictions than the more complex CIEDE2000 (ΔE*₀₀) metric. It is concluded that colorimetry can be a viable non-destructive tool for predicting mechanical degradation, but its application is limited to specific high-chroma materials, precluding a universal model based entirely on colorimetry. Full article

25 pages, 6086 KB

Open AccessArticle

Timer-Based Digitization of Analog Sensors Using Ramp-Crossing Time Encoding

by Gabriel Bravo, Ernesto Sifuentes, Geu M. Puentes-Conde, Francisco Enríquez-Aguilera, Juan Cota-Ruiz, Jose Díaz-Roman and Arnulfo Castro

Technologies 2026, 14(1), 72; https://doi.org/10.3390/technologies14010072 (registering DOI) - 18 Jan 2026

Abstract

This work presents a time-domain analog-to-digital conversion method in which the amplitude of a sensor signal is encoded through its crossing instants with a periodic ramp. The proposed architecture departs from conventional ADC and PWM demodulation approaches by shifting quantization entirely to the [...] Read more.

This work presents a time-domain analog-to-digital conversion method in which the amplitude of a sensor signal is encoded through its crossing instants with a periodic ramp. The proposed architecture departs from conventional ADC and PWM demodulation approaches by shifting quantization entirely to the time domain, enabling waveform reconstruction using only a ramp generator, an analog comparator, and a timer capture module. A theoretical framework is developed to formalize the voltage-to-time mapping, derive expressions for resolution and error, and identify the conditions ensuring monotonicity and single-crossing behavior. Simulation results demonstrate high-fidelity reconstruction for both periodic and non-periodic signals, including real photoplethysmographic (PPG) waveforms, with errors approaching the theoretical quantization limit. A hardware implementation on a PSoC 5LP microcontroller confirms the practicality of the method under realistic operating conditions. Despite ramp nonlinearity, comparator delay, and sensor noise, the system achieves effective resolutions above 12 bits using only native mixed-signal peripherals and no conventional ADC. These results show that accurate waveform reconstruction can be obtained from purely temporal information, positioning time-encoded sensing as a viable alternative to traditional amplitude-based conversion. The minimal analog front end, low power consumption, and scalability of timer-based processing highlight the potential of the proposed approach for embedded instrumentation, distributed sensor nodes, and biomedical monitoring applications. Full article

12 pages, 239 KB

Open AccessArticle

An Ubuntu Pentecostal Perspective of Pan-Africanism and African Identity

by Abraham Modisa Mkhondo Mzondi

Religions 2026, 17(1), 112; https://doi.org/10.3390/rel17010112 (registering DOI) - 18 Jan 2026

Abstract

Clarke provides a critical analysis of Pentecostalism as a tool for attaining the theological and political objectives of Pan-Africanism. However, this seems to suggest that, at least, African Pentecostals and African Pentecostal researchers may not be aware of the African Union’s (AU) Agenda [...] Read more.

Clarke provides a critical analysis of Pentecostalism as a tool for attaining the theological and political objectives of Pan-Africanism. However, this seems to suggest that, at least, African Pentecostals and African Pentecostal researchers may not be aware of the African Union’s (AU) Agenda 2013, or, at worst, they find no interest in engaging with Agenda 2063 if they are aware it exists. Using religion and politics—particularly Pentecostalism and politics—as a framework, this article notes that there are some points of convergence between their praxis and some of the seven aspirations of Agenda 2063. It addresses this phenomenon by using Mzondi’s Ubuntu Pentecostalism as a theological lens to reflect on how some of the actions and praxis of African Pentecostals relate to the African Union’s Agenda 2063. Ubuntu Pentecostalism holds to a holistic view of life and embraces William Seymour’s Pentecostalism, influenced by an African worldview, and either embraces or denounces ancestral veneration. The latter form of Ubuntu Pentecostalism is used in this article and placed alongside Pan-Africanism and African identity to provide a perspective on the third and fifth aspirations of the African Union’s Agenda 2063. The article further shows that (a) although African Pentecostals may not be aware of or do not bother to engage with the AU’s Agenda 2063, (b) their praxis and actions either support or contradict the third and fifth aspirations discussed in the article. Full article

(This article belongs to the Section Religions and Theologies)

14 pages, 1242 KB

Open AccessArticle

The Influence of Injection Modes on CO₂ Flooding and Storage in Low-Permeability Reservoirs

by Wencheng Han

Energies 2026, 19(2), 480; https://doi.org/10.3390/en19020480 (registering DOI) - 18 Jan 2026

Abstract

Low-permeability reservoirs have poor reservoir properties and are difficult to develop by conventional water flooding. CO₂ flooding can significantly improve oil recovery while achieving carbon storage, and is widely recognized as an effective solution for the development of low-permeability oil reservoirs. In [...] Read more.

Low-permeability reservoirs have poor reservoir properties and are difficult to develop by conventional water flooding. CO₂ flooding can significantly improve oil recovery while achieving carbon storage, and is widely recognized as an effective solution for the development of low-permeability oil reservoirs. In order to address the lack of a comparative quantitative analysis of the tradeoff between oil recovery factor, CO₂ storage rate, and total CO₂ storage volume for the main injection modes in low-permeability reservoirs, this study systematically evaluated the performance of CO₂-enhanced oil recovery (EOR) and geological storage under different pressures and injection modes through core flooding experiments. The results indicate that displacement pressure and injection strategy significantly influence the CO₂ flooding performance. Continuous miscible flooding (30 MPa) substantially reduced the displacement pressure differential (maximum 6.1 MPa) and achieved the highest oil recovery (78.96%) and the greatest CO₂ storage capacity (5916 cm³). Miscible WAG flooding effectively delayed gas breakthrough (extended to 1.90 pore volumes), homogenized the displacement front, and yielded the best overall outcome: the highest ultimate oil recovery (83.8%) coupled with the optimal CO₂ storage rate (89.1%). The study further reveals that the pre-breakthrough stage is critical for contributing to oil recovery and achieving efficient storage, regardless of the injection modes. These findings clarify the technical characteristics and applicable conditions of different injection modes, providing crucial theoretical insights and practical guidance for optimizing CO₂ EOR and storage projects in low-permeability reservoirs. Full article

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16 pages, 6066 KB

Open AccessArticle

Validation and Improvement of a Rapid, CRISPR-Cas-Free RPA-PCRD Strip Assay for On-Site Genomic Surveillance and Quarantine of Wheat Blast

by Dipali Rani Gupta, Shamfin Hossain Kasfy, Julfikar Ali, Farin Tasnova Hia, M. Nazmul Hoque, Mahfuz Rahman and Tofazzal Islam

J. Fungi 2026, 12(1), 73; https://doi.org/10.3390/jof12010073 (registering DOI) - 18 Jan 2026

Abstract

As an emerging threat to global food security, wheat blast necessitates the development of a rapid and field-deployable detection system to facilitate early diagnosis, enable effective management, and prevent its further spread to new regions. In this study, we aimed to validate and [...] Read more.

As an emerging threat to global food security, wheat blast necessitates the development of a rapid and field-deployable detection system to facilitate early diagnosis, enable effective management, and prevent its further spread to new regions. In this study, we aimed to validate and improve a Recombinase Polymerase Amplification coupled with PCRD lateral flow detection (RPA-PCRD strip assay) kit for the rapid and specific identification of Magnaporthe oryzae pathotype Triticum (MoT) in field samples. The assay demonstrated exceptional sensitivity, detecting as low as 10 pg/µL of target DNA, and exhibited no cross-reactivity with M. oryzae Oryzae (MoO) isolates and other major fungal phytopathogens under the genera of Fusarium, Bipolaris, Colletotrichum, and Botrydiplodia. The method successfully detected MoT in wheat leaves as early as 4 days post-infection (DPI), and in infected spikes, seeds, and alternate hosts. Furthermore, by combining a simplified polyethylene glycol-NaOH method for extracting DNA from plant samples, the entire RPA-PCRD strip assay enabled the detection of MoT within 30 min with no specialized equipment and high technical skills at ambient temperature (37–39 °C). When applied to field samples, it successfully detected MoT in naturally infected diseased wheat plants from seven different fields in a wheat blast hotspot district, Meherpur, Bangladesh. Training 52 diverse stakeholders validated the kit’s field readiness, with 88% of trainees endorsing its user-friendly design. This method offers a practical, low-cost, and portable point-of-care diagnostic tool suitable for on-site genomic surveillance, integrated management, seed health testing, and quarantine screening of wheat blast in resource-limited settings. Furthermore, the RPA-PCRD platform serves as an early warning modular diagnostic template that can be readily adapted to detect a wide array of phytopathogens by integrating target-specific genomic primers. Full article

(This article belongs to the Special Issue Integrated Management of Plant Fungal Diseases—2nd Edition)

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17 pages, 3200 KB

Open AccessArticle

The Self-Assembling Peptide P₁₁-4 Induces the Expression of Mineralization-Related Genes in Odontoblasts Independently of Metabolic Alterations

by Leticia Martins Pereira, Marina Damasceno e Souza de Carvalho Chiari, Diego Mauro Carneiro Pereira, Regina Maria Puppin-Rontani and Fábio Dupart Nascimento

J. Funct. Biomater. 2026, 17(1), 50; https://doi.org/10.3390/jfb17010050 (registering DOI) - 18 Jan 2026

Abstract

(1) Background: The synthetic eleven-amino acid peptide P₁₁-4, derived from DMP-1, self-assembles into β-sheet tapes, ribbons, fibrils, and fibers that form a 3D matrix enriched with calcium-binding sites. This study investigated whether P₁₁-4 modulates gene and protein expression or [...] Read more.

(1) Background: The synthetic eleven-amino acid peptide P₁₁-4, derived from DMP-1, self-assembles into β-sheet tapes, ribbons, fibrils, and fibers that form a 3D matrix enriched with calcium-binding sites. This study investigated whether P₁₁-4 modulates gene and protein expression or induces adverse metabolic alterations in odontoblast-like cells. (2) Methods: MDPC-23 cells were cultured under standard conditions and stimulated with different concentrations of P₁₁-4, followed by assessments of cell viability using the MTT assay, proliferation and migration, cytoplasmic calcium kinetics, reactive oxygen species (ROS) production, osteogenic differentiation-related gene expression via PCR array, and expression of the pro-inflammatory cytokine interleukin-6 (IL-6) using confocal microscopy and flow cytometry. (3) Results: The MTT assay showed that P₁₁-4 at 6.3, 12.6, and 25.2 µmol/L was non-cytotoxic and did not alter MDPC-23 cell proliferation or migration. Only the 25.2 µmol/L concentration induced a detectable Ca²⁺ influx and a slight increase in ROS. Among the 84 genes examined, P₁₁-4 at 6.3 µmol/L upregulated 79 genes, including transcription factors, signaling molecules, and extracellular matrix-related proteins. Furthermore, P₁₁-4 did not increase IL-6 expression under any condition tested. (4) Conclusion: P₁₁-4 markedly modulates mineralization-associated gene regulation without causing metabolic damage in odontoblast-like cells. Full article

(This article belongs to the Special Issue Biomaterials in Restorative Dentistry and Endodontics (2nd Edition))

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21 pages, 502 KB

Open AccessArticle

Electrodermal Response Patterns and Emotional Engagement Under Continuous Algorithmic Video Stimulation: A Multimodal Biometric Analysis

by Carolina Del-Valle-Soto, Violeta Corona, Jesus Gomez Romero-Borquez, David Contreras-Tiscareno, Diego Sebastian Montoya-Rodriguez, Jesus Abel Gutierrez-Calvillo, Bernardo Sandoval and José Varela-Aldás

Technologies 2026, 14(1), 70; https://doi.org/10.3390/technologies14010070 (registering DOI) - 18 Jan 2026

Abstract

Excessive use of short-form video platforms such as TikTok has raised growing concerns about digital addiction and its impact on young users’ emotional well-being. This study examines the relationship between continuous TikTok exposure and emotional engagement in young adults aged 20–23 through a [...] Read more.

Excessive use of short-form video platforms such as TikTok has raised growing concerns about digital addiction and its impact on young users’ emotional well-being. This study examines the relationship between continuous TikTok exposure and emotional engagement in young adults aged 20–23 through a multimodal experimental design. The purpose of this research is to determine whether emotional engagement increases, remains stable, or declines during prolonged exposure and to assess the degree of correspondence between facially inferred engagement and physiological arousal. To achieve this, multimodal biometric data were collected using the iMotions platform, integrating galvanic skin response (GSR) sensors and facial expression analysis via Affectiva’s AFFDEX SDK 5.1. Engagement levels were binarized using a logistic transformation, and a binomial test was conducted. GSR analysis, merged with a 50 ms tolerance, revealed no significant differences in skin conductance between engaged and non-engaged states. Findings indicate that although TikTok elicits strong initial emotional engagement, engagement levels significantly decline over time, suggesting habituation and emotional fatigue. The results refine our understanding of how algorithm-driven, short-form content affects users’ affective responses and highlight the limitations of facial metrics as sole indicators of physiological arousal. Implications for theory include advancing multimodal models of emotional engagement that account for divergences between expressivity and autonomic activation. Implications for practice emphasize the need for ethical platform design and improved digital well-being interventions. The originality and value of this study lie in its controlled experimental approach that synchronizes facial and physiological signals, offering objective evidence of the temporal decay of emotional engagement during continuous TikTok use and underscoring the complexity of measuring affect in highly stimulating digital environments. Full article

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19 pages, 27717 KB

Open AccessArticle

Acoustic–Electric Conversion Characteristics of a Quadruple Parallel-Cavity Helmholtz Resonator-Based Triboelectric Nanogenerator (4C–HR TENG)

by Xinjun Li, Chaoming Huang and Zhilin Wang

Processes 2026, 14(2), 341; https://doi.org/10.3390/pr14020341 (registering DOI) - 18 Jan 2026

Abstract

This paper presents the design and fabrication of a triboelectric nanogenerator based on a Quadruple Parallel-cavity Helmholtz Resonator (4C–HR TENG) for the efficient harvesting of noise energy in marine engine room environments. The device utilizes sound waves to drive periodic contact and separation [...] Read more.

This paper presents the design and fabrication of a triboelectric nanogenerator based on a Quadruple Parallel-cavity Helmholtz Resonator (4C–HR TENG) for the efficient harvesting of noise energy in marine engine room environments. The device utilizes sound waves to drive periodic contact and separation between polytetrafluoroethylene (PTFE) particles in the resonant cavity and the vibrating diaphragm as well as the upper electrode plate, thereby converting sound energy into mechanical energy and finally into electrical energy. The device consists of an acoustic waveguide with a length of 350 mm and both width and height of 60 mm, along with a Helmholtz Resonator with a diameter of 60 mm and a height of 40 mm. Experimental results indicate that under resonance conditions with a sound pressure level of 109.8 dB and a frequency of 110 Hz, the device demonstrates excellent output performance, achieving a peak output voltage of 250 V and a current of 4.85 μA. We analyzed and investigated the influence mechanism of key parameters (filling ratio, sound pressure level, the height between the electrode plates, and particle size) on the output performance. Through COMSOL Multiphysics simulation analysis, the sound pressure enhancement effect and the characteristic of concentrated diaphragm center displacement at the first-order resonance frequency were revealed, verifying the advantage of the four-cavity structure in terms of energy distribution uniformity. In practical applications, the minimum responsive sound pressure level corresponding to the operating frequency range of the 4C–HR TENG was determined. The output power reaches a maximum of 0.27 mW at a load resistance of 50 MΩ. At a sound pressure level of 115.1 dB, the device can charge a 1 μF capacitor to 4.73 V in just 32 s and simultaneously illuminate 180 LEDs in real-time, demonstrating its potential for environmental noise energy harvesting and micro-energy supply applications. This study provides new insights and experimental evidence for the efficient recovery of noise energy. Full article

(This article belongs to the Section Energy Systems)

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22 pages, 3741 KB

Open AccessArticle

HiLTS©: Human-in-the-Loop Therapeutic System: A Wireless-enabled Digital Neuromodulation Testbed for Brainwave Entrainment

by Arfan Ghani

Technologies 2026, 14(1), 71; https://doi.org/10.3390/technologies14010071 (registering DOI) - 18 Jan 2026

Abstract

Epileptic seizures arise from abnormally synchronized neural activity and remain a major global health challenge, affecting more than 50 million people worldwide. Despite advances in pharmacological interventions, a significant proportion of patients continue to experience uncontrolled seizures, underscoring the need for alternative neuromodulation [...] Read more.

Epileptic seizures arise from abnormally synchronized neural activity and remain a major global health challenge, affecting more than 50 million people worldwide. Despite advances in pharmacological interventions, a significant proportion of patients continue to experience uncontrolled seizures, underscoring the need for alternative neuromodulation strategies. Rhythmic neural entrainment has recently emerged as a promising mechanism for disrupting pathological synchrony, but most existing systems rely on complex analog electronics or high-power stimulation hardware. This study investigates a proof-of-concept digital custom-designed chip that generates a stable 6 Hz oscillation capable of imposing a stable rhythmic pattern onto digitized seizure-like EEG dynamics. Using a publicly available EEG seizure dataset, we extracted and averaged analog seizure waveforms, digitized them to emulate neural front-ends, and directly interfaced the digitized signals with digital output recordings acquired from the chip using a Saleae Logic analyser. The chip’s pulse train was resampled and low-pass-reconstructed to produce an analog 6 Hz waveform, allowing direct comparison between seizure morphology, its digitized representation, and the entrained output. Frequency-domain and time-domain analyses demonstrate that the chip imposes a narrow-band 6 Hz rhythm that overrides the broadband spectral profile of seizure activity. These results provide a proof-of-concept for low-power digital custom-designed entrainment as a potential pathway toward simplified, wearable neuromodulation device for future healthcare diagnostics. Full article

16 pages, 382 KB

Open AccessArticle

Are Maternal Adverse Childhood Experiences Associated with Their Preschool Children’s Sleep Disruptions? Longitudinal Mediation Through Mothers’ Depressive Symptoms and Children’s Screen Time

by Stefan Kurbatfinski, Lalith Nandakumar, Janelle Boram Lee, Gerald F. Giesbrecht and Nicole Letourneau

Children 2026, 13(1), 139; https://doi.org/10.3390/children13010139 (registering DOI) - 18 Jan 2026

Abstract

Background: Children of mothers exposed to adverse childhood experiences (ACEs) may be at increased risk of sleep disruptions, such as night waking, due to potential suboptimal caregiving or living conditions. Mothers’ ACEs are also associated with maternal depressive symptoms, which in turn are [...] Read more.

Background: Children of mothers exposed to adverse childhood experiences (ACEs) may be at increased risk of sleep disruptions, such as night waking, due to potential suboptimal caregiving or living conditions. Mothers’ ACEs are also associated with maternal depressive symptoms, which in turn are associated with children’s screen time and sleep disruptions, revealing relevant, but unexplored, mediation pathways. This Canadian study investigated if mothers’ ACEs were associated with their 5-year-old children’s sleep disruptions (1) directly and (2) indirectly through independent or serial mediation via maternal depressive symptoms and/or children’s screen time. Methods: Data (n = 622; maternal mean age 32.3 years, 88.4% white) came from the longitudinal APrON Study. ACEs were measured 1 year postpartum. Mother’s depressive symptoms were measured across prenatal and postnatal timepoints. Children’s evening screen time (i.e., number of days in a week children engaged in one hour of screen time before bedtime) and sleep disruptions (number of days in a week their child wakes up multiple times) were measured at 5 years postpartum using adapted scales (52.9% male). PROCESS was used to assess for mediation. Results: Mothers’ ACEs had an indirect effect on their children’s sleep disruptions through mothers’ mean depressive symptoms (effect = 0.018, 95% CI [0.006, 0.034]), but not through children’s screen time. No other effects (i.e., direct, total) were observed. Conclusions: Although replication studies are warranted, this novel study reveals that the effects of maternal ACEs on children’s sleep disruptions may operate indirectly with effects potentiated through maternal depressive symptoms, thus serving as a target for intervention. Full article

(This article belongs to the Section Pediatric Mental Health)

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17 pages, 3205 KB

Open AccessArticle

Graphene/Chalcogenide Heterojunctions for Enhanced Electric-Field-Sensitive Dielectric Performance: Combining DFT and Experimental Study

by Bo Li, Nanhui Zhang, Yuxing Lei, Mengmeng Zhu and Haitao Yang

Nanomaterials 2026, 16(2), 128; https://doi.org/10.3390/nano16020128 (registering DOI) - 18 Jan 2026

Abstract

Electric-field-sensitive dielectrics play a crucial role in electric field induction sensing and related capacitive conversion, with interfacial polarization and charge accumulation largely determining the signal output. This paper introduces graphene/transition metal dichalcogenide (TMD) (MoSe₂, MoS₂, and WS₂) [...] Read more.

Electric-field-sensitive dielectrics play a crucial role in electric field induction sensing and related capacitive conversion, with interfacial polarization and charge accumulation largely determining the signal output. This paper introduces graphene/transition metal dichalcogenide (TMD) (MoSe₂, MoS₂, and WS₂) heterojunctions as functional fillers to enhance the dielectric response and electric-field-induced voltage output of flexible polydimethylsiloxane (PDMS) composites. Density functional theory (DFT) calculations were used to evaluate the stability of the heterojunctions and interfacial electronic modulation, including binding behavior, charge redistribution, and Fermi level-referenced band structure/total density of states (TDOS) characteristics. The calculations show that the graphene/TMD interface is primarily controlled by van der Waals forces, exhibiting negative binding energy and significant interfacial charge rearrangement. Based on these theoretical results, graphene/TMD heterojunction powders were synthesized and incorporated into polydimethylsiloxane (PDMS). Structural characterization confirmed the presence of face-to-face interfacial contacts and consistent elemental co-localization within the heterojunction filler. Dielectric spectroscopy analysis revealed an overall improvement in the dielectric constant of the composite materials while maintaining a stable loss trend within the studied frequency range. More importantly, calibrated electric field induction tests (based on pure PDMS) showed a significant enhancement in the voltage response of all heterojunction composite materials, with the WS₂-G/PDMS system exhibiting the best performance, exhibiting an electric-field-induced voltage amplitude 7.607% higher than that of pure PDMS. This work establishes a microscopic-to-macroscopic correlation between interfacial electronic modulation and electric-field-sensitive dielectric properties, providing a feasible interface engineering strategy for high-performance flexible dielectric sensing materials. Full article

(This article belongs to the Section 2D and Carbon Nanomaterials)

18 pages, 3557 KB

Open AccessArticle

Longitudinal Landscape of Long Flu and Long COVID

by Ming Zheng

COVID 2026, 6(1), 21; https://doi.org/10.3390/covid6010021 (registering DOI) - 18 Jan 2026

Abstract

Influenza is typically framed as an acute respiratory infection, yet accumulating evidence suggests that—like SARS-CoV-2—it may trigger persistent, multi-organ morbidity consistent with a post-acute infection syndrome (“long flu”). Leveraging the nationwide FinnGen registry infrastructure, we conducted a temporally stratified disease-wide association study (DWAS) [...] Read more.

Influenza is typically framed as an acute respiratory infection, yet accumulating evidence suggests that—like SARS-CoV-2—it may trigger persistent, multi-organ morbidity consistent with a post-acute infection syndrome (“long flu”). Leveraging the nationwide FinnGen registry infrastructure, we conducted a temporally stratified disease-wide association study (DWAS) to map antecedent risk factors and long-term sequelae following clinically diagnosed influenza and COVID-19. We assembled an exposed cohort comprising 9204 individuals with influenza (ICD-10 J09–J11) and 4,258 individuals with COVID-19 (ICD-10 U072) recorded in specialist inpatient/outpatient care between 1998 and 2021, and an unexposed comparator cohort of 420,005 individuals with no recorded influenza or pneumonia (J09–J18) across their available medical history. Across harmonized clinical endpoints, we fitted age- and sex-adjusted Cox proportional hazards models and controlled for multiple testing using a stringent false discovery rate threshold (FDR-adjusted p<0.001), further interrogating temporal persistence within 1-, 5-, and 15-year windows. The DWAS revealed that both infections are associated with broad, system-spanning disease signatures extending beyond the respiratory tract, including circulatory, neurological, metabolic, musculoskeletal, digestive, mental/behavioural, ocular, and oncologic endpoints. Predisposition analyses demonstrated that infection risk is concentrated in individuals with substantial pre-existing multimorbidity, most prominently cardiovascular disease, alongside cardiometabolic, respiratory, renal, neuropsychiatric, and inflammatory conditions. Post-infection analyses identified a durable burden of incident multi-system morbidity after influenza, with particularly robust and persistent cardiovascular and neurological signatures—encompassing thromboembolic disease and major adverse cardiovascular outcomes, as well as migraine, neurodegenerative disorders, and depression—together with metabolic and renal sequelae that, in subsets, extended across multi-year horizons. Collectively, these longitudinal findings reframe influenza as a systemic event embedded within a chronic disease continuum, motivate recognition of “long flu” as a clinically meaningful post-viral risk landscape, and support intensified prevention and risk-stratified surveillance strategies alongside analogous efforts for long COVID. Full article

(This article belongs to the Special Issue Post-Acute Infection Syndromes: Lessons from Long COVID and Long Flu)

33 pages, 19776 KB

Open AccessArticle

Multiparametric Vibration Diagnostics of Machine Tools Within a Digital Twin Framework Using Machine Learning

by Andrey Kurkin, Yuri Kabaldin, Maksim Zhelonkin, Sergey Mancerov, Maksim Anosov and Dmitriy Shatagin

Appl. Sci. 2026, 16(2), 982; https://doi.org/10.3390/app16020982 (registering DOI) - 18 Jan 2026

Abstract

In the context of the digital transformation of industrial production, the need for intelligent maintenance and repair systems capable of ensuring reliable operation of machine-tool equipment without operator involvement is growing. This present study reviews the current state and future development of diagnostic [...] Read more.

In the context of the digital transformation of industrial production, the need for intelligent maintenance and repair systems capable of ensuring reliable operation of machine-tool equipment without operator involvement is growing. This present study reviews the current state and future development of diagnostic and condition-monitoring systems for metalworking machine tools. A review of international standards and existing solutions from domestic and international vendors in vibration diagnostics has been conducted. Particular attention is paid to non-intrusive vibration diagnostics, digital twins, multiparametric analysis methods, and neural network approaches to failure prediction. The architecture of the developed system is presented. The concept of the system is developed in full compliance with Russian and international standards of vibration diagnostics. At its core, the comprehensive digital twin relies on machine learning methods. The proposed architecture is a predictive-maintenance system built on interconnected digital twin realizations: the dynamic machine passport of a unit, operational data, and a comprehensive digital twin of the machine-tool equipment. The potential of neuromorphic computing on a hardware platform is being considered as a promising element for local-condition classification and emergency protection. At the current development stage, the operating principle has been demonstrated along with the integration into the control loop. The system is now at the beginning of laboratory testing. It demonstrates capabilities for comprehensive assessment of the equipment’s technical condition based on multiparametric data, short-term vibration trend forecasting using a Long Short-Term Memory network, and state classification using a Multilayer Perceptron model. The results of the system’s testing on a turning machining center have been analyzed. Full article

(This article belongs to the Special Issue Vibration-Based Diagnostics and Condition Monitoring)

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34 pages, 15440 KB

Open AccessArticle

Spatial Identification and Evolutionary Analysis of Production–Living–Ecological Space—Taking Lincang City as an Example

by Tingyue Deng, Dongyang Hou and Cansong Li

Land 2026, 15(1), 179; https://doi.org/10.3390/land15010179 (registering DOI) - 18 Jan 2026

Abstract

Optimizing the “production–living–ecological” space (PLES) is critical for achieving the UN Sustainable Development Goals (SDGs), particularly in ecologically sensitive mountainous border regions. This study investigates the spatial patterns and dynamic evolution of PLES in Lincang City (2010–2020) to reveal the trade-offs between development [...] Read more.

Optimizing the “production–living–ecological” space (PLES) is critical for achieving the UN Sustainable Development Goals (SDGs), particularly in ecologically sensitive mountainous border regions. This study investigates the spatial patterns and dynamic evolution of PLES in Lincang City (2010–2020) to reveal the trade-offs between development and conservation. Methodologically, we proposed a coupling-coordination-based grid-level PLES identification framework. This framework integrates the coupling coordination degree model (CCDM) directly into the functional classification process at a 600 m grid scale—a resolution selected to balance the capture of spatial heterogeneity with the maintenance of functional integrity in complex terrains. Spatiotemporal dynamics were further quantified using transition matrices and a dimension-based landscape metric system. The results reveal that (a) ecological space and production–living–ecological space represent the predominant categories in the study area. During the study period, ecological space continued to decrease, while production–living space increased steadily, and other PLES categories showed only marginal variations. (b) Mutual transitions among PLES types primarily occurred among ecological space, production–ecological space, and production–living–ecological space. These transitions intensified markedly between 2015 and 2020 compared to the 2010–2015 period. (c) From 2010 to 2020, the landscape in Lincang evolved towards lower ecological risk yet higher fragmentation. High fragmentation values, often associated with grassland, cropland, and forested areas, were evenly distributed across northeastern and northwestern regions. Likewise, high landscape dominance and isolation appeared in these regions as well as in the southeast. Conversely, landscape disturbance remained relatively uniform throughout the city, with lower values detected in forested land. Full article

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18 pages, 1950 KB

Open AccessReview

Molecular and Cellular Mechanisms of Cardioplegic Protection in Surgical Myocardial Revascularization

by Dejan M. Lazović, Milica Karadžić Kočica, Dragan Ivanišević, Vojkan Aleksić, Mladen J. Kočica, Danko Grujić, Jovana M. Mihajlović, Dragan Cvetković and Stefan A. Juričić

Cells 2026, 15(2), 173; https://doi.org/10.3390/cells15020173 (registering DOI) - 18 Jan 2026

Abstract

Coronary artery bypass grafting (CABG) remains the gold standard for patients with advanced multivessel coronary artery disease. Optimal myocardial protection versus ischemia during reversible and controlled cardiac arrest is a cornerstone of successful outcomes. Myocardial ischemia represents a state of reduced coronary perfusion [...] Read more.

Coronary artery bypass grafting (CABG) remains the gold standard for patients with advanced multivessel coronary artery disease. Optimal myocardial protection versus ischemia during reversible and controlled cardiac arrest is a cornerstone of successful outcomes. Myocardial ischemia represents a state of reduced coronary perfusion with oxygenated blood, insufficient to meet the metabolic demands of the myocardium. Conventional cardioplegic solutions offer controlled and reversible cardiac arrest while actively modulating the molecular and cellular mechanisms that mediate ischemia–reperfusion injury. Cardioplegia dramatically elongates the reversible period of ischemic injury and restricts cardiomyocyte death by shutting down electromechanical activity, lowering metabolic demand, stabilizing ionic homeostasis, protecting mitochondrial integrity, and slowing oxidative stress and inflammatory signaling. During ischemia, cardiomyocytes shift from aerobic to anaerobic metabolism, resulting in adenosine triphosphate (ATP) depletion, loss of ionic homeostasis and calcium overload that activate proteases, phospholipases and membrane damage. Reperfusion restores oxygen supply and prevents irreversible necrosis but paradoxically initiates additional injury in marginally viable myocardium. The reoxygenation phase induces excessive production of reactive oxygen species (ROS), endothelial dysfunction and a strong inflammatory response mediated by neutrophils, platelets and cytokines. Mitochondrial dysfunction and opening of the mitochondrial permeability transition pore (mPTP) further amplify oxidative stress and inflammation, and trigger apoptosis and necroptosis. Understanding these intertwined cellular and molecular mechanisms remains essential for identifying novel therapeutic targets aimed at reducing reperfusion injury and improving myocardial recovery after ischemic events, particularly in coronary surgery. Full article

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23 pages, 1032 KB

Open AccessReview

Effects of Cannabidiol on Bone Health: A Comprehensive Scoping Review

by Shabbir Adnan Shakir and Kok-Yong Chin

Biomedicines 2026, 14(1), 208; https://doi.org/10.3390/biomedicines14010208 (registering DOI) - 18 Jan 2026

Abstract

Background/objectives: Cannabidiol (CBD) is a non-psychoactive constituent of Cannabis sativa, which has potential skeletal benefits through modulation of bone cell function and inflammatory signalling. However, evidence of its effects and mechanisms in bone health remains fragmented. This scoping review summarised the current [...] Read more.

Background/objectives: Cannabidiol (CBD) is a non-psychoactive constituent of Cannabis sativa, which has potential skeletal benefits through modulation of bone cell function and inflammatory signalling. However, evidence of its effects and mechanisms in bone health remains fragmented. This scoping review summarised the current findings on the impact of CBD on bone outcomes and its mechanisms of action. Methods: A systematic search of PubMed, Scopus, and Web of Science was conducted in October 2025 for original studies published in English, with the primary objective of examining the effects of CBD on bone health, regardless of study design. After applying inclusion and exclusion criteria, 24 primary studies were included. Data on model design, CBD formulation, treatment parameters, bone-related outcomes, and proposed mechanisms were extracted and analysed descriptively. Results: Among the studies included, eleven demonstrated beneficial effects of CBD on bone formation, mineralisation, callus quality, or strength; eleven showed mixed outcomes; and two demonstrated no apparent benefit. Previous studies have shown that CBD suppresses bone resorption by reducing osteoclast differentiation and activity while promoting osteoblast proliferation and matrix deposition. Mechanistically, CBD’s effects involve activation of cannabinoid receptor 2, modulation of the receptor activator of nuclear factor-κB ligand/osteoprotegerin pathway, and regulation of osteoblastogenic and osteoclastogenic signalling through bone morphogenetic protein, Wnt, mitogen-activated protein kinase, nuclear factor-κB, and peroxisome proliferator-activated receptor signalling. The anti-inflammatory and antioxidant actions of CBD further contribute to a favourable bone microenvironment. Conclusions: Preclinical evidence suggests that CBD has a bone-protective role through multifaceted pathways that enhance osteoblast function and suppress osteoclast activity. Nevertheless, robust human trials are necessary to confirm its efficacy, determine its optimal dosing, and clarify its long-term safety. Full article

(This article belongs to the Special Issue Musculoskeletal Diseases: From Molecular Basis to Therapy—3rd Edition)

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13 pages, 2810 KB

Open AccessArticle

Two Cultivars of Peanut (Arachis hypogaea) Show Different Responses to Iron Deficiency

by Lei Chen, Zifei Liu, Lei Zhou and Hong Wang

Curr. Issues Mol. Biol. 2026, 48(1), 99; https://doi.org/10.3390/cimb48010099 (registering DOI) - 18 Jan 2026

Abstract

Background: Peanut is susceptible to iron (Fe) deficiency, particularly in calcareous soils. However, comparative studies on the adaptive mechanisms of different peanut cultivars to Fe deficiency remain limited. This study aimed to investigate the physiological and molecular responses of two distinct peanut [...] Read more.

Background: Peanut is susceptible to iron (Fe) deficiency, particularly in calcareous soils. However, comparative studies on the adaptive mechanisms of different peanut cultivars to Fe deficiency remain limited. This study aimed to investigate the physiological and molecular responses of two distinct peanut cultivars to Fe deprivation and to identify the key traits contributing to differential Fe efficiency. Methods: Two peanut cultivars, LH11 and YZ9102, were cultivated under Fe-sufficient and Fe-deficient conditions, using both hydroponic and pot-based soil culture systems. Multiple parameters were assessed, including visual symptomology, biomass, tissue Fe concentration, active Fe in leaves, chlorophyll (Chl) content (SPAD value), net photosynthetic rate (Pn), Chl fluorescence (Fv/Fm), rhizosphere pH, root ferric chelate reductase (FCR) activity, and the relative expression of two Fe-acquisition-related genes (AhIRT1 and AhFRO1) via qRT-PCR. Results: Cultivar YZ9102 exhibited more severe Fe deficiency chlorosis symptoms, which also appeared earlier than in LH11, under both cultivation systems. Under Fe deficiency, YZ9102 showed significantly lower Chl content, Pn, and Fv/Fm compared to LH11. In contrast, LH11 demonstrated a greater capacity for rhizosphere acidification and maintained significantly higher root FCR activity under Fe-limited conditions. Gene expression analysis revealed that Fe deficiency induced the up-regulation of AhIRT1 and AhFRO1 in the roots of LH11, while their transcript levels were suppressed or unchanged in YZ9102. Conclusions: The peanut cultivar LH11 possesses superior tolerance to Fe deficiency compared to YZ9102. This enhanced tolerance is attributed to a synergistic combination of traits: the maintenance of photosynthetic performance, efficient rhizosphere acidification, heightened root Fe³⁺ reduction capacity, and the positive transcriptional regulation of key Fe uptake genes. These findings provide crucial insights for the selection and breeding of Fe-efficient peanut varieties for cultivation in Fe-deficient environments. Full article

(This article belongs to the Section Molecular Plant Sciences)

14 pages, 2042 KB

Open AccessArticle

Effects of Different Application Strategies of Copper-Loaded Montmorillonite on Growth, Intestinal Histology, and Rearing-Water Quality in Penaeus monodon

by Jieyi Wang, Yangyang Ding, Falin Zhou, Jianzhi Shi, Qibin Yang, Yundong Li, Jianhua Huang, Lishi Yang, Xueliang Sun and Song Jiang

Fishes 2026, 11(1), 63; https://doi.org/10.3390/fishes11010063 (registering DOI) - 18 Jan 2026

Abstract

Penaeus monodon is widely cultured in Asia; however, intensive farming practices often result in water-quality deterioration and compromised production performance. Copper-loaded montmorillonite (Cu-MMT) is a functional additive with adsorption and antimicrobial properties, yet the relative effectiveness of different application strategies remains insufficiently evaluated. [...] Read more.

Penaeus monodon is widely cultured in Asia; however, intensive farming practices often result in water-quality deterioration and compromised production performance. Copper-loaded montmorillonite (Cu-MMT) is a functional additive with adsorption and antimicrobial properties, yet the relative effectiveness of different application strategies remains insufficiently evaluated. In this study, 270 shrimp were assigned to three treatments: a control group (KZ), water application of Cu-MMT (PZ), and dietary inclusion of Cu-MMT (BZ). Juvenile Penaeus monodon with an initial body weight of 3.25 ± 0.15 g were used in the trial. Growth performance, intestinal histology, and rearing-water quality were assessed over a 56-day culture period. Shrimp in the BZ group exhibited a significantly higher weight gain rate (311.88 ± 38.17%) and survival rate (88.04%) than those in the KZ (247.45 ± 32.82%; 76.67%) and PZ (286.49 ± 29.78%; 83.33%) groups (p < 0.05). Intestinal histological observations revealed treatment-associated differences in morphology, with more pronounced intestinal enlargement observed in the PZ group, whereas the BZ group exhibited a more moderate intestinal architecture. Water-quality analyses showed that dietary Cu-MMT supplementation was associated with higher dissolved oxygen levels and lower concentrations of total ammonia nitrogen, sulfide, and dissolved iron, particularly during the later stages of the experiment. Overall, these results indicate that dietary inclusion of Cu-MMT provides more favorable outcomes than water application in improving growth performance and rearing-water quality in P. monodon culture under the experimental conditions tested. These findings highlight the importance of application strategy when evaluating functional additives in shrimp aquaculture. Full article

(This article belongs to the Special Issue Diet and Intestinal Microbiota of Fish)

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28 pages, 1593 KB

Open AccessArticle

How to Spot an Entrepreneurial University? A Student-Focused Perspective on Competencies—The Case of Greece

by Vasiliki Chronaki, Angeliki Karagiannaki and Dimosthenis Kotsopoulos

Educ. Sci. 2026, 16(1), 145; https://doi.org/10.3390/educsci16010145 (registering DOI) - 18 Jan 2026

Abstract

As universities increasingly work towards the adoption of their third mission—fostering entrepreneurship and innovation—the concept of the Entrepreneurial University (EntUni) emphasizes the need to cultivate a defined set of entrepreneurial competencies in students, such as opportunity recognition, risk-taking, perseverance, self-efficacy, and adaptability. The [...] Read more.

As universities increasingly work towards the adoption of their third mission—fostering entrepreneurship and innovation—the concept of the Entrepreneurial University (EntUni) emphasizes the need to cultivate a defined set of entrepreneurial competencies in students, such as opportunity recognition, risk-taking, perseverance, self-efficacy, and adaptability. The purpose of this study is to identify which entrepreneurial competencies are most critical for student readiness within the context of an Entrepreneurial University. However, limited consensus remains on which competencies are most essential. This study identifies the entrepreneurial competencies most critical for students within an Entrepreneurial University context through a mixed-methods approach. A student survey assesses self-perceived competencies; a stakeholder survey captures the perspectives of faculty, industry experts, and entrepreneurs; and qualitative interviews with industry professionals explore best practices for competency development. Findings reveal six core competencies that EntUnis should help students cultivate: proactiveness, perseverance, grit, risk propensity, self-efficacy, and entrepreneurial intention. Industry experts further highlight the importance of teamwork, ethical and sustainable thinking, and ambiguity tolerance—competencies often underdeveloped in academic environments. The study also identifies a disconnect between entrepreneurial education and practical application, with many students demonstrating high entrepreneurial intention but limited participation in start-up activities. These insights offer actionable implications for educators, policymakers, and university administrators. Overall, the study highlights the importance of experiential learning, academia-industry collaboration, and structured competency-building to enhance entrepreneurial readiness. By addressing these gaps, EntUnis can better equip students to drive innovation, economic growth, and societal impact. Full article

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