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20 pages, 4791 KiB  
Article
Satellite-Measured Suspended Particulate Matter Flux and Freshwater Flux in the Yellow Sea and East China Sea
by Wei Shi and Menghua Wang
Remote Sens. 2025, 17(15), 2726; https://doi.org/10.3390/rs17152726 - 6 Aug 2025
Abstract
Traditionally, the surface suspended particulate matter (SPM) and freshwater fluxes have been computed using in situ SPM, salinity, and current measurements or through the numerical modeling. In this study, satellite-derived SPM concentration, ocean current, and sea surface salinity (SSS) are used to demonstrate [...] Read more.
Traditionally, the surface suspended particulate matter (SPM) and freshwater fluxes have been computed using in situ SPM, salinity, and current measurements or through the numerical modeling. In this study, satellite-derived SPM concentration, ocean current, and sea surface salinity (SSS) are used to demonstrate the capability to characterize and quantify the surface SPM flux and freshwater flux in the Yellow Sea (YS) and East China Sea (ECS). The different routes for SPM and freshwater to transport from the coastal region to the interior ECS are identified. The seasonal and interannual SPM and freshwater fluxes from the coastal region of the ECS are further characterized and quantified. The average SPM flux reaches ~0.3–0.4 g m−2 s−1 along the route. The SPM and the freshwater fluxes in the region show different seasonality. The intensified SPM flux from the ECS coast to the offshore in winter is one order higher than the SPM flux in summer, while the offshore freshwater flux peaks in summer and weakens significantly in winter. Particularly, we found that the SPM and SSS features in the ECS changed in response to the 2020 summer Yangtze River flood event. These spatial and temporal changes for SPM and SSS in the ECS in the 2020 summer and early autumn were attributed to the anomalous surface SPM and freshwater fluxes in the same period. Full article
(This article belongs to the Special Issue Remote Sensing for Ocean-Atmosphere Interaction Studies)
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15 pages, 4944 KiB  
Article
The Geochemical Characteristics of the Fatty Acids in the Core Sediments in the Northern South Yellow Sea
by Jinxian He, Xiaoli Zhang, Ruihua Ma, Zhengxin Huang, Juhao Li, Peilin Sun and Jiayao Song
J. Mar. Sci. Eng. 2025, 13(8), 1511; https://doi.org/10.3390/jmse13081511 - 5 Aug 2025
Abstract
The geochemistry of the fatty acids in the modern sediments in the Northern South Yellow Sea is still poorly studied, and studies on the geochemistry of the fatty acids in relatively long-core sediment samples are lacking. Thus, the fatty acids in the core [...] Read more.
The geochemistry of the fatty acids in the modern sediments in the Northern South Yellow Sea is still poorly studied, and studies on the geochemistry of the fatty acids in relatively long-core sediment samples are lacking. Thus, the fatty acids in the core sediments in the Northern South Yellow Sea were separated and identified to study their components and distribution characteristics, and the sources of organic matter and the early diagenetic evolution of the fatty acids in the sediments were discussed. The results show that saturated straight-chain fatty acids (methyl ester) have the highest content in the core sediments in the Northern South Yellow Sea, which account for 83.89% of the total fatty acids (methyl ester). nC16:0 is dominant, accounting for 30.48% of the n-saturated fatty acids (methyl ester). Unsaturated fatty acids (methyl ester) account for 7.59% of the total fatty acids (methyl ester). Binary unsaturated fatty acids (methyl ester) can only be detected in some samples, which are low in content and dominated by C18:2. Based on the components and distribution of the fatty acids (methyl ester) in the core sediments in the Northern South Yellow Sea, combined with the characteristics of other lipid biomarker compounds, the actual geological background, and previous research results, it is considered that the sources of organic matter in the core sediments are marine–terrestrial mixed materials, with terrestrial materials dominating. The fatty acids’ (methyl ester) CPI, the relative content of short-chain saturated fatty acids (methyl ester), and the unsaturated fatty acids (methyl ester) in the core sediments show non-obvious variation as the burial depth increases, reflecting that the fatty acids in the core sediments are strongly degraded at the early diagenetic stage, and this degradation is controlled by various complicated factors. Full article
(This article belongs to the Section Geological Oceanography)
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14 pages, 2239 KiB  
Article
Marsupenaeus japonicus HSP90’s Function Under Low Temperature Stress
by Xueqiong Bian, Xianyun Ren, Shaoting Jia, Tian Gao, Junxia Wang, Jiajia Wang, Ping Liu, Jian Li and Jitao Li
Biology 2025, 14(8), 966; https://doi.org/10.3390/biology14080966 - 1 Aug 2025
Viewed by 206
Abstract
Molecular chaperones, especially heat shock proteins (HSPs) have vital functions in cells’ responses to stress. Here, we cloned and sequenced the complete complementary DNA encoding HSP90 (MjHSP90) from the shrimp Marsupenaeus japonicus. The MjHSP90 cDNA comprised 3162 bp, including a [...] Read more.
Molecular chaperones, especially heat shock proteins (HSPs) have vital functions in cells’ responses to stress. Here, we cloned and sequenced the complete complementary DNA encoding HSP90 (MjHSP90) from the shrimp Marsupenaeus japonicus. The MjHSP90 cDNA comprised 3162 bp, including a 2172 bp coding region encoding a 724 amino acid-protein (predicted molecular mass = 83.12 kDa). Homology and phylogenetic analyses showed that MjHSP90 was highly conserved and most homologous to Litopenaeus vannamei HSP90. MjHSP90 is expressed in all tested tissues, with high expression in gill tissue and the hepatopancreas. Cold stress significantly upregulated MjHSP90 expression in the gill and hepatopancreas (p < 0.05). Following RNA interference knockdown of MjHSP90, the cold stress-related death rate of the shrimp increased significantly, accompanied by significantly upregulated expression of apoptosis-related genes Mjcaspase-3 and Mjbcl-2 (p < 0.05) and an increase in the number of apoptotic cells. The results indicated that MjHSP90 might play a pivotal role in the shrimp’s immune response to cold stress. Full article
(This article belongs to the Section Biochemistry and Molecular Biology)
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28 pages, 5779 KiB  
Article
Regional Wave Spectra Prediction Method Based on Deep Learning
by Yuning Liu, Rui Li, Wei Hu, Peng Ren and Chao Xu
J. Mar. Sci. Eng. 2025, 13(8), 1461; https://doi.org/10.3390/jmse13081461 - 30 Jul 2025
Viewed by 232
Abstract
The wave spectrum, as a key statistical feature describing wave energy distribution, is crucial for understanding wave propagation mechanisms and supporting ocean engineering applications. This study, based on ERA5 reanalysis spectrum data, proposes a model combining CNN and xLSTM for rapid gridded wave [...] Read more.
The wave spectrum, as a key statistical feature describing wave energy distribution, is crucial for understanding wave propagation mechanisms and supporting ocean engineering applications. This study, based on ERA5 reanalysis spectrum data, proposes a model combining CNN and xLSTM for rapid gridded wave spectrum prediction over the Bohai and Yellow Seas domain. It uses 2D gridded spectrum data rather than a spectrum at specific points as input and analyzes the impact of various input factors at different time lags on wave development. The results show that incorporating water depth and mean sea level pressure significantly reduces errors. The model performs well across seasons with the seasonal spatial average root mean square error (SARMSE) of spectral energy remaining below 0.040 m2·s and RMSEs for significant wave height (SWH) and mean wave period (MWP) of 0.138 m and 1.331 s, respectively. At individual points, the spectral density bias is near zero, correlation coefficients range from 0.95 to 0.98, and the peak frequency RMSE is between 0.03 and 0.04 Hz. During a typical cold wave event, the model accurately reproduces the energy evolution and peak frequency shift. Buoy observations confirm that the model effectively tracks significant wave height trends under varying conditions. Moreover, applying a frequency-weighted loss function enhances the model’s ability to capture high-frequency spectral components, further improving prediction accuracy. Overall, the proposed method shows strong performance in spectrum prediction and provides a valuable approach for regional wave spectrum modeling. Full article
(This article belongs to the Section Physical Oceanography)
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14 pages, 2700 KiB  
Article
Seasonal Spatial Distribution Patterns of the Sand Crab Ovalipes punctatus (De Haan 1833) in the Southern Yellow and East China Seas and Predictions from Various Climate Scenarios
by Min Xu, Jianzhong Ling, Haisu Zheng, Xiaojing Song, Zunlei Liu and Huiyu Li
Biology 2025, 14(8), 947; https://doi.org/10.3390/biology14080947 - 28 Jul 2025
Viewed by 342
Abstract
In the past two decades, little information has been updated to understand the resource status of the crab species Ovalipes punctatus in the East China Sea Region. In this study, we conducted surveys in 2018 and 2019 to identify the seasonal spatial distribution [...] Read more.
In the past two decades, little information has been updated to understand the resource status of the crab species Ovalipes punctatus in the East China Sea Region. In this study, we conducted surveys in 2018 and 2019 to identify the seasonal spatial distribution patterns of the economically important sand crab Ovalipes punctatus (De Haan 1833) in the southern Yellow and East China Seas. In the study area, the largest biomass of crabs was observed in the fishing grounds of Dasha and the Yangtze River mouth, and the second largest biomass was detected in the Jiangwai-Zhouwai area. Seasonally, the total biomass order in these areas was summer > autumn & winter > spring, and the mean average individual weight order was spring & summer > winter > autumn. These findings provided maps of the seasonal spatial distribution pattern of the species across seasons, which were then used in climate-change scenario models. Model predictions suggested that O. punctatus might migrate northward and offshore under climate warming conditions, and that the climate scenario SSP585-2100 might be the most negative case, respectively, for the habitat area of gain% minus loss%. These data can be used to develop robust and systematic regional fisheries resource management policies that consider adaptation measures to address the impact of environmental and climate change along China’s coasts and other areas in the world. Full article
(This article belongs to the Special Issue Global Fisheries Resources, Fisheries, and Carbon-Sink Fisheries)
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16 pages, 993 KiB  
Review
The Application of Digital Twin Technology in the Development of Intelligent Aquaculture: Status and Opportunities
by Jianlei Chen, Yong Xu, Hao Li, Xinguo Zhao, Yang Su, Chunhao Qi, Keming Qu and Zhengguo Cui
Fishes 2025, 10(8), 363; https://doi.org/10.3390/fishes10080363 - 25 Jul 2025
Viewed by 294
Abstract
Aquaculture is vital for global food security but faces challenges like disease, water quality control, and resource optimization. Digital twin technology, a real-time virtual replica of physical aquaculture systems, emerges as a transformative solution. By integrating sensors and data analytics, it enables monitoring [...] Read more.
Aquaculture is vital for global food security but faces challenges like disease, water quality control, and resource optimization. Digital twin technology, a real-time virtual replica of physical aquaculture systems, emerges as a transformative solution. By integrating sensors and data analytics, it enables monitoring and optimization of water quality, feed efficiency, fish health, and operations. This review explores the current adoption status of digital twins in aquaculture, highlighting applications in real-time monitoring and system optimization. It addresses key implementation challenges, including data integration and scalability, and identifies emerging opportunities for advancing sustainable, intelligent aquaculture practices. Full article
(This article belongs to the Special Issue Application of Artificial Intelligence in Aquaculture)
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19 pages, 2677 KiB  
Article
Role of StAR Gene in Sex Steroid Hormone Regulation and Gonadal Development in Ark Shell Scapharca broughtonii
by Wenjing Wang, Zhihong Liu, Huaying Zhang, Zheying Gao, Sudong Xia, Xiujun Sun, Liqing Zhou, Zhuanzhuan Li, Peizhen Ma and Biao Wu
Biology 2025, 14(8), 925; https://doi.org/10.3390/biology14080925 - 23 Jul 2025
Viewed by 433
Abstract
This study elucidates the role of the steroidogenic acute regulatory protein (StAR) in sex steroid hormone dynamics and the gonadal development of the commercially important marine bivalve ark shell Scapharca broughtonii. The sequence of the StAR gene was obtained and [...] Read more.
This study elucidates the role of the steroidogenic acute regulatory protein (StAR) in sex steroid hormone dynamics and the gonadal development of the commercially important marine bivalve ark shell Scapharca broughtonii. The sequence of the StAR gene was obtained and verified from the transcriptome of ark shell, then the tissue localization and expression pattern during the gonad development of the StAR gene were detected by in situ hybridization and quantitative real-time PCR, respectively. Additionally, the concentrations of three critical sex steroid hormones (progesterone, testosterone, and estradiol) were measured throughout gonadal development using enzyme-linked immunosorbent assay (ELISA). The results showed that the length of the coding region of StAR was 1446 bp, encoding 481 amino acids. The results of qRT-PCR showed that the expression of the StAR gene varied with gonadal development, increased from the early active stage to the development stage, and decreased from the mature stage to the spent stage. Notably, the expression level in ovaries was higher than that in testes, suggesting the potential involvement of StAR in sex differentiation and gonadal development. Additionally, the results indicated that progesterone, testosterone, and estradiol accounted for 80%, 10%, and 10% of the total hormone content in the gonads, respectively. Correlation analysis revealed a highly significant strong positive correlation between progesterone/estradiol levels and StAR gene expression, demonstrating that StAR serves as a key regulator in sex steroid hormone biosynthesis. These findings provide crucial molecular evidence for StAR-mediated steroidogenesis in bivalve reproduction, offering fundamental insights into invertebrate endocrinology. Full article
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18 pages, 1698 KiB  
Review
Bioactive Peptides Derived from Tuna: Screening, Extraction, Bioactivity, and Mechanism of Action
by Jing-an Cheng, Di Wang, Gang Yu, Shengjun Chen, Zhenhua Ma, Ya Wei, Xue Zhao, Chunsheng Li, Yueqi Wang, Yi Zhang, Rong Cao and Yongqiang Zhao
Mar. Drugs 2025, 23(7), 293; https://doi.org/10.3390/md23070293 - 21 Jul 2025
Viewed by 438
Abstract
Peptides play a crucial role in the development of pharmaceuticals and functional foods. Multiple studies have shown that natural bioactive peptides possess antioxidant, antihypertensive, anti-tumor, and anti-inflammatory activities. Marine bioactive peptides, especially those sourced from fish, constitute a substantial reservoir of these molecules. [...] Read more.
Peptides play a crucial role in the development of pharmaceuticals and functional foods. Multiple studies have shown that natural bioactive peptides possess antioxidant, antihypertensive, anti-tumor, and anti-inflammatory activities. Marine bioactive peptides, especially those sourced from fish, constitute a substantial reservoir of these molecules. Although considerable research has been undertaken on fish-derived peptides, studies specifically concerning those from tuna are limited. Tuna, a marine fish of high nutritional value, generates substantial by-product waste during fishing and processing. Therefore, it is essential to conduct an evaluation of the advancements in study on tuna-derived active peptides and to offer a perspective on the direction of future investigations. This review integrates prospective bioactive peptides derived from tuna and reports contemporary strategies for their investigation, including extraction, purification, screening, identification, and activity evaluation procedures, including Yeast Surface Display (YSD) and molecular docking. This review seeks to promote the continued investigation and application of bioactive peptides derived from tuna. Full article
(This article belongs to the Special Issue High-Value-Added Resources Recovered from Marine By-Products)
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17 pages, 1009 KiB  
Article
Analysis of Five Biogenic Amines in Foods on the Chinese Market and Estimation of Acute Histamine Exposure from Fermented Foods in the Chinese Population
by Pei Cao, Mengmeng Gao, Dongmei Huang, Xiaomin Xu, Zhujun Liu, Qing Liu, Yang Lu, Feng Pan, Zhaoxin Li, Jinfang Sun, Lei Zhang and Pingping Zhou
Foods 2025, 14(14), 2550; https://doi.org/10.3390/foods14142550 - 21 Jul 2025
Viewed by 309
Abstract
Biogenic amines (BAs) are frequently detected in seafood products, wines, and fermented foods, and they pose potential risks to human health. The current study analyzed the concentrations of five common BAs in seafood, fermented food, and complementary food for infants and children (fish [...] Read more.
Biogenic amines (BAs) are frequently detected in seafood products, wines, and fermented foods, and they pose potential risks to human health. The current study analyzed the concentrations of five common BAs in seafood, fermented food, and complementary food for infants and children (fish sausage, canned complementary food for infants containing fish and shrimp ingredients, and fish floss) in China and estimated the acute health risks of histamine (HIS) from fermented foods in Chinese consumers. Among all the samples analyzed, HIS exhibited the highest detection rate (51.9%), followed by PUT (50.1%), and the detection rate of TRY (12.5%) was the lowest. The total average concentration of the five BAs across major food categories revealed that fermented bean curd had the highest total concentration of BAs (816.8 mg/kg), followed by shrimp (383.2 mg/kg) and cheese (328.0 mg/kg). In contrast, samples of complementary food for infants and children contained the lowest concentrations of BAs; the total average concentration of the five BAs was 12.0 mg/kg. The point assessment results showed that acute dietary exposure to HIS was highest from cheese (76.2 mg/d), followed by fermented bean products (74.5 mg/d). Furthermore, the probability assessment indicated that the probability of acute health risks from exposure to HIS was 0.44% for fermented bean product consumers and 0.014% for cheese consumers, respectively. Thus, for the general consumer, the probability of acute health risks caused by HIS in seafood and fermented foods is low. However, individuals with high consumption of cheese and fermented bean products may need to be concerned. Full article
(This article belongs to the Section Food Quality and Safety)
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17 pages, 4550 KiB  
Article
Spatiotemporal Characteristics and Associated Circulation Features of Summer Extreme Precipitation in the Yellow River Basin
by Degui Yao, Xiaohui Wang and Jinyu Wang
Atmosphere 2025, 16(7), 892; https://doi.org/10.3390/atmos16070892 - 21 Jul 2025
Viewed by 180
Abstract
By utilizing daily precipitation data from 400 meteorological stations in the Yellow River Basin (YRB) of China, atmospheric and oceanic reanalysis data, this study investigates the climatological characteristics, leading modes, and relationships with atmospheric circulation and sea surface temperature (SST) of summer extreme [...] Read more.
By utilizing daily precipitation data from 400 meteorological stations in the Yellow River Basin (YRB) of China, atmospheric and oceanic reanalysis data, this study investigates the climatological characteristics, leading modes, and relationships with atmospheric circulation and sea surface temperature (SST) of summer extreme precipitation in the YRB from 1981 to 2020 through the extreme precipitation metrics and Empirical Orthogonal Function (EOF) analysis. The results indicate that both the frequency and intensity of extreme precipitation exhibit an eastward and southward increasing pattern in terms of climate state, with regions of higher precipitation showing greater interannual variability. When precipitation in the YRB exhibits a spatially coherent enhancement pattern, high latitudes exhibits an Eurasian teleconnection wave train that facilitates the southward movement of cold air. Concurrently, the northward extension of the Western Pacific subtropical high (WPSH) enhances moisture transport from low latitudes to the YRB, against the backdrop of a transitioning SST pattern from El Niño to La Niña. When precipitation in the YRB shows a “south-increase, north-decrease” dipole pattern, the southward-shifted Ural high and westward-extended WPSH converge cold air and moist in the southern YRB region, with no dominant SST drivers identified. Full article
(This article belongs to the Section Meteorology)
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10 pages, 3839 KiB  
Article
Sound Production Characteristics of the Chorus Produced by Small Yellow Croaker (Larimichthys polyactis) in Coastal Cage Aquaculture
by Young Geul Yoon, Hansoo Kim, Sungho Cho, Sunhyo Kim, Yun-Hwan Jung and Donhyug Kang
J. Mar. Sci. Eng. 2025, 13(7), 1380; https://doi.org/10.3390/jmse13071380 - 21 Jul 2025
Viewed by 306
Abstract
Recent advances in passive acoustic monitoring (PAM) have markedly improved the ability to study marine soundscapes by enabling long-term, non-invasive monitoring of biological sounds across large spatial and temporal scales. Among aquatic organisms, fish are primary contributors to biophony, producing sounds associated with [...] Read more.
Recent advances in passive acoustic monitoring (PAM) have markedly improved the ability to study marine soundscapes by enabling long-term, non-invasive monitoring of biological sounds across large spatial and temporal scales. Among aquatic organisms, fish are primary contributors to biophony, producing sounds associated with feeding, reproduction, and social behavior. However, the majority of previous research has focused on individual vocalizations, with limited attention to collective acoustic phenomena such as fish choruses. This study quantitatively analyzes choruses produced by the small yellow croaker (Larimichthys polyactis), an ecologically and commercially important species in the Northwest Pacific Ocean. Using power spectral density (PSD) analysis, we examined long-term underwater recordings from a sea cage containing approximately 2000 adult small yellow croakers. The choruses were centered around ~600 Hz and exhibited sound pressure levels 15–20 dB higher at night than during the day. These findings highlight the ecological relevance of fish choruses and support their potential use as indicators of biological activity. This study lays the foundation for incorporating fish choruses into soundscape-based PAM frameworks to enhance biodiversity and habitat monitoring. Full article
(This article belongs to the Special Issue Advanced Research in Marine Environmental and Fisheries Acoustics)
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18 pages, 9131 KiB  
Article
The Primary Cultivation of Oogonial Stem Cells from Black Rockfish (Sebastes schlegelii): Morphology and Transcriptome Landscape
by Jingjing Zhang, Lei Lin, Shengyu Zhu, Yanming Zhang, Caichao Dong, Yu Yang, Yuyan Liu, Xuwen Cao, Yangbin He, Honglong Ji, Bo Meng, Qian Wang and Changwei Shao
Int. J. Mol. Sci. 2025, 26(14), 6772; https://doi.org/10.3390/ijms26146772 - 15 Jul 2025
Viewed by 287
Abstract
Black rockfish (Sebastes schlegelii) is a marine ovoviviparous teleost that exhibits significant sexual dimorphism, with females growing faster and reaching larger sizes than males. Establishing stable oogonial stem cells (OSCs) is critical for understanding germline stem cell dynamics and facilitating all-female [...] Read more.
Black rockfish (Sebastes schlegelii) is a marine ovoviviparous teleost that exhibits significant sexual dimorphism, with females growing faster and reaching larger sizes than males. Establishing stable oogonial stem cells (OSCs) is critical for understanding germline stem cell dynamics and facilitating all-female breeding. In this study, we successfully isolated and cultured OSCs from S. schlegelii for 12 passages. These cells exhibited alkaline phosphatase activity, expressed germline marker genes (ddx4, cdh1, klf4), and maintained a diploid karyotype (2n = 48). Transcriptomic comparisons between early (P3) and late (P12) passages revealed significant metabolic dysfunction and cell cycle arrest in the late-passage cells. Specifically, the down-regulation of glutathione-related and glycolysis-related genes (gstm3, gstt1, mgst3, gsta1, gsta4, gsto1, gapdh) and key mitotic regulators (cdk1, chk1, cdk4, e2f3, ccne2, ccnb1) suggested that metabolic imbalance contributes to oxidative stress, resulting in cell cycle inhibition and eventual senescence. This study provides a marine fish model for investigating metabolism-cell cycle interactions in germline stem cells and lays the foundation for future applications in germ cell transplantation and all-female breeding. Full article
(This article belongs to the Special Issue Molecular Research on Reproductive Physiology and Endocrinology)
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19 pages, 863 KiB  
Review
Microplastic Pollution in China’s Aquatic Systems: Spatial Distribution, Transport Pathways, and Controlling Strategies
by Zhancheng Wu, Juzhuang Wang, Shengwang Yu, Qian Sun and Yulai Han
Microplastics 2025, 4(3), 41; https://doi.org/10.3390/microplastics4030041 - 3 Jul 2025
Viewed by 862
Abstract
Microplastics (MPs) have emerged as a critical environmental challenge in China’s aquatic ecosystems, driven by rapid industrialization and population growth. This review synthesizes recent findings on the abundance, morphology, and polymer types of MPs in China’s freshwater systems (rivers, lakes, reservoirs) and coastal [...] Read more.
Microplastics (MPs) have emerged as a critical environmental challenge in China’s aquatic ecosystems, driven by rapid industrialization and population growth. This review synthesizes recent findings on the abundance, morphology, and polymer types of MPs in China’s freshwater systems (rivers, lakes, reservoirs) and coastal marine environments. Spatial analysis reveals significant variability in MP abundance, ranging from 0.1 items/L in Tibet’s Lalu Wetland to 30.8 items/L in Beijing’s Qinghe River, with polypropylene (PP) and polyethylene (PE) dominating polymer profiles. Coastal regions exhibit distinct contamination patterns, with the Yellow Sea (5.3 ± 2.0 items/L) and the South China Sea (180 ± 80 items/m3) showing the highest MP loads, primarily as fibers and fragments. Fluvial transport, atmospheric deposition, and coastal anthropogenic activities (e.g., fisheries, tourism) are identified as major pathways for marine MP influx. Secondary MPs from degraded plastics and primary MPs from industrial/domestic effluents pose synergistic risks through the adsorption of heavy metals and organic pollutants. Human exposure routes—ingestion, inhalation, and dermal contact—are linked to inflammatory, metabolic, and carcinogenic health outcomes. Policy interventions, including bans on microbeads and non-degradable plastics, demonstrate progress in pollution mitigation. This work underscores the urgency of integrated source control, advanced wastewater treatment, and transboundary monitoring to address MP contamination in aquatic ecosystems. Full article
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3 pages, 170 KiB  
Editorial
Editorial for the “Genetic Studies of Fish” Special Issue
by Yang Liu
Genes 2025, 16(7), 752; https://doi.org/10.3390/genes16070752 - 27 Jun 2025
Viewed by 271
Abstract
Fish represent the most diverse vertebrate group, with over 32,000 species [...] Full article
16 pages, 3566 KiB  
Article
Effects of Dietary β-Carotene on the Gonadal Color, Pigmentation, and Regulation Mechanisms in Sea Urchin Strongylocentrotus Intermedius
by Weixiao Di, Yinuo Zhang, Huinan Zuo, Haijing Liu, Lina Wang, Jun Ding, Yaqing Chang and Rantao Zuo
Fishes 2025, 10(7), 304; https://doi.org/10.3390/fishes10070304 - 24 Jun 2025
Viewed by 375
Abstract
This study aims to clarify the dose–response relationship between dietary β-carotene levels and gonadal pigment deposition and regulation mechanisms related to the carotenoid synthesis of Strongylocentrotus intermedius based on a 60-day feeding trial and subsequent transcriptome analysis. Adult sea urchins (initial weight: 9.33 [...] Read more.
This study aims to clarify the dose–response relationship between dietary β-carotene levels and gonadal pigment deposition and regulation mechanisms related to the carotenoid synthesis of Strongylocentrotus intermedius based on a 60-day feeding trial and subsequent transcriptome analysis. Adult sea urchins (initial weight: 9.33 ± 0.21 g) of three cages were given one of the dry feeds with different doses of β-carotene (0 mg/kg, 150 mg/kg, 300 mg/kg) or fresh kelp (Saccharina japonica). The results indicated that the weight gain rate (WGR) of sea urchins increased with the addition of β-carotene, with that of the C300 group being markedly higher than that of the C0 group. The addition of β-carotene significantly improved the redness (a*) and yellowness (b*) values of the gonads, with sea urchins in the C300 group exhibiting closest gonad coloration to those in the kelp-fed group. Meanwhile, β-carotene and echinenone in the gonads of the C300 group showed the highest contents, reaching 1.96 μg/kg and 11.97 μg/kg, respectively. Several differential genes, enriched in the pathways of steroid biosynthesis, oxidative phosphorylation, and ubiquitination, were screened based on transcriptome analysis. Real-time PCR further demonstrated that β-carotene significantly upregulated the expression of cholesterol 25-hydroxylase (CH25H), NADH dehydrogenase subunit 1 (ND1), NADH dehydrogenase subunit 2 (ND2), and NADH dehydrogenase subunit 4 (ND4) while it downregulated the expression of 24-dehydrocholesterol reductase (DHCR24). These results showed that 300 mg/kg β-carotene significantly increased the WGR, redness, and yellowness values, as well as the contents of β-carotene and echinenone in the gonads of S. intermedius. On the one hand, dietary β-carotene increased NADH enzyme activity, which participates in echinenone synthesis by donating electrons for the transformation of β-carotene to echinenone synthesis. On the other hand, the addition of β-carotene inhibited cholesterol synthesis by increasing the expression of CH25H and decreasing the expression of DHCR24, which could in turn increase the fluidity and permeability of the cell membranes and the transport efficiency of β-carotene and echinenone from the digestive tract to the gonads. These results provided fundamental insights into the production of sea urchin gonads with market-favored colors. Full article
(This article belongs to the Special Issue Advances in Aquaculture Feed Additives)
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