Marsupenaeus japonicus HSP90’s Function Under Low Temperature Stress
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Shrimp Rearing
2.2. Cloning of the MjHSP90 cDNA
2.3. Sequence Characterization and Phylogenetic Tree Construction
2.4. Cold Stress and Sample Acquisition
2.5. Analysis of MjHSP90 Tissue Expression and Low-Temperature Expression Pattern
2.6. Analysis of the Localization of MjHSP90 in Gill and Hepatopancreas Tissue
2.6.1. Preparation of Frozen Sections
2.6.2. Synthesis of the RNA Probe
2.6.3. Fluorescence In Situ Hybridization (FISH)
2.7. The Impact of MjHSP90 Knockdown on the Expression Levels of Genes Associated with the Mitochondrial Apoptosis Pathway
2.7.1. Double-Stranded RNA (dsRNA) Synthesis
2.7.2. Detection of dsMjHSP90 Interference Efficiency
2.7.3. M. japonicus Mortality When Treated with Cold Stress After MjHSP90 Knockdown
2.7.4. Assessment of Gene Expression Associated with the Mitochondrial Apoptosis Pathway Following MjHSP90 Knockdown
2.8. TUNEL Assay Detection of Apoptosis
2.9. Statistical Considerations
3. Results
3.1. Analysis of the MjHSP90 Sequence
3.2. Multiple Sequence Alignment
3.3. Phylogenetics
3.4. Tissue Distribution of MjHSP90 in M. japonicus
3.5. Expression Pattern of MjHSP90 in Cold Stress-Treated Shrimp
3.6. The Efficiency of the MjHSP90 dsRNA Interference
3.7. M. japonicus Mortality When Treated with Cold Stress After MjHSP90 Knockdown
3.8. Differential Expression of M. japonicus Apoptotic Pathway Genes Under Cold Stress After MjHSP90 Knockdown
3.9. Cell Apoptosis Following MjHSP90 Knockdown
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bian, X.; Ren, X.; Jia, S.; Gao, T.; Wang, J.; Wang, J.; Liu, P.; Li, J.; Li, J. Marsupenaeus japonicus HSP90’s Function Under Low Temperature Stress. Biology 2025, 14, 966. https://doi.org/10.3390/biology14080966
Bian X, Ren X, Jia S, Gao T, Wang J, Wang J, Liu P, Li J, Li J. Marsupenaeus japonicus HSP90’s Function Under Low Temperature Stress. Biology. 2025; 14(8):966. https://doi.org/10.3390/biology14080966
Chicago/Turabian StyleBian, Xueqiong, Xianyun Ren, Shaoting Jia, Tian Gao, Junxia Wang, Jiajia Wang, Ping Liu, Jian Li, and Jitao Li. 2025. "Marsupenaeus japonicus HSP90’s Function Under Low Temperature Stress" Biology 14, no. 8: 966. https://doi.org/10.3390/biology14080966
APA StyleBian, X., Ren, X., Jia, S., Gao, T., Wang, J., Wang, J., Liu, P., Li, J., & Li, J. (2025). Marsupenaeus japonicus HSP90’s Function Under Low Temperature Stress. Biology, 14(8), 966. https://doi.org/10.3390/biology14080966