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19 pages, 1159 KiB  
Article
Determining the Effect of Different Concentrations of Spent Coffee Grounds on the Metabolomic Profile of Swiss Chard
by Thabiso Motseo and Lufuno Ethel Nemadodzi
Int. J. Plant Biol. 2025, 16(3), 88; https://doi.org/10.3390/ijpb16030088 (registering DOI) - 7 Aug 2025
Abstract
In the coming decades, the agricultural system will predictably rely on organic material to produce crops and maintain food security. Currently, the use of inorganic fertilizers to grow crops and vegetables, such as Swiss chard, spinach, and lettuce, is on the rise and [...] Read more.
In the coming decades, the agricultural system will predictably rely on organic material to produce crops and maintain food security. Currently, the use of inorganic fertilizers to grow crops and vegetables, such as Swiss chard, spinach, and lettuce, is on the rise and has been proven to be detrimental to the soil in the long run. Hence, there is a growing need to use organic waste material, such as spent coffee grounds (SCGs), to grow crops. Spent coffee grounds are made of depleted coffee beans that contain important soluble compounds. This study aimed to determine the influence of different levels (0.32 g, 0.63 g, 0.92 g, and 1.20 g) of spent coffee grounds on the metabolomic profile of Swiss chard. The 1H-nuclear magnetic resonance (NMR) results showed that Swiss chard grown with different levels of SCGs contains a total of 10 metabolites, which included growth-promoting metabolites (trehalose; betaine), defense mechanism metabolites (alanine; cartinine), energy-reserve metabolites (sucrose; 1,6 Anhydro-β-D-glucose), root metabolites (thymine), stress-related metabolites (2-deoxyadenosine), caffeine metabo-lites (1,3 Dimethylurate), and body-odor metabolites (trimethylamine). Interestingly, caprate, with the abovementioned metabolites, was detected in Swiss chard grown without the application of SCGs. The findings of the current study suggest that SCGs are an ideal organic material for growing Swiss chard for its healthy metabolites. Full article
30 pages, 7051 KiB  
Review
Review of Material-Handling Challenges in Energy Production from Biomass and Other Solid Waste Materials
by Tong Deng, Vivek Garg and Michael S. A. Bradley
Energies 2025, 18(15), 4194; https://doi.org/10.3390/en18154194 - 7 Aug 2025
Abstract
Biomass and other solid wastes create potential environmental and health hazards in our modern society. Conversion of the wastes into energy presents a promising avenue for sustainable energy generation. However, the feasibility of the approach is limited by the challenges in material handling [...] Read more.
Biomass and other solid wastes create potential environmental and health hazards in our modern society. Conversion of the wastes into energy presents a promising avenue for sustainable energy generation. However, the feasibility of the approach is limited by the challenges in material handling because of the special properties of the materials. Despite their critical importance, the complexities of material handling often evade scrutiny until operational implementation. This paper highlights the challenges inherent in standard solid material-handling processes, preceded by a concise review of common solid waste typologies and their physical properties, particularly those related to biomass and biowastes. It delves into the complexities of material flow, storage, compaction, agglomeration, separation, transport, and hazard management. Specialised characterisation techniques essential for informed process design are also discussed to mitigate operational risks. In conclusion, this paper emphasises the necessity of a tailored framework before the establishment of any further conversion processes. Given the heterogeneous nature of biomaterials, material-handling equipment must demonstrate adaptability to accommodate the substantial variability in material properties in large-scale production. This approach aims to enhance feasibility and efficacy of any energy conversion initiatives by using biomass or other solid wastes, thereby advancing sustainable resource utilisation and environmental stewardship. Full article
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13 pages, 778 KiB  
Article
Relationship Between Chronic Wasting Disease (CWD) Infection and Pregnancy Probability in Wild Female White-Tailed Deer (Odocoileus virginianus) in Northern Illinois, USA
by Jameson Mori, Nelda A. Rivera, William Brown, Daniel Skinner, Peter Schlichting, Jan Novakofski and Nohra Mateus-Pinilla
Pathogens 2025, 14(8), 786; https://doi.org/10.3390/pathogens14080786 - 7 Aug 2025
Abstract
White-tailed deer (Odocoileus virginianus) are a cervid species native to the Americas with ecological, social, and economic significance. Managers must consider several factors when working to maintain the health and sustainability of these wild herds, including reproduction, particularly pregnancy and recruitment [...] Read more.
White-tailed deer (Odocoileus virginianus) are a cervid species native to the Americas with ecological, social, and economic significance. Managers must consider several factors when working to maintain the health and sustainability of these wild herds, including reproduction, particularly pregnancy and recruitment rates. White-tailed deer have a variable reproductive capacity, with age, health, and habitat influencing this variability. However, it is unknown whether chronic wasting disease (CWD) impacts reproduction and, more specifically, if CWD infection alters a female deer’s probability of pregnancy. Our study addressed this question using data from 9783 female deer culled in northern Illinois between 2003 and 2023 as part of the Illinois Department of Natural Resources’ ongoing CWD management program. Multilevel Bayesian logistic regression was employed to quantify the relationship between pregnancy probability and covariates like maternal age, deer population density, and date of culling. Maternal infection with CWD was found to have no significant effect on pregnancy probability, raising concerns that the equal ability of infected and non-infected females to reproduce could make breeding, which inherently involves close physical contact, an important source of disease transmission between males and females and females and their fawns. The results also identified that female fawns (<1 year old) are sensitive to county-level deer land cover utility (LCU) and deer population density, and that there was no significant difference in how yearlings (1–2 years old) and adult (2+ years old) responded to these variables. Full article
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19 pages, 1835 KiB  
Article
Methods for Enhancing Energy and Resource Efficiency in Sunflower Oil Production: A Case Study from Bulgaria
by Penka Zlateva, Angel Terziev, Nikolay Kolev, Martin Ivanov, Mariana Murzova and Momchil Vasilev
Eng 2025, 6(8), 195; https://doi.org/10.3390/eng6080195 - 6 Aug 2025
Abstract
The rising demand for energy resources and industrial goods presents significant challenges to sustainable development. Sunflower oil, commonly utilized in the food sector, biofuels, and various industrial applications, is notably affected by this demand. In Bulgaria, it serves as a primary source of [...] Read more.
The rising demand for energy resources and industrial goods presents significant challenges to sustainable development. Sunflower oil, commonly utilized in the food sector, biofuels, and various industrial applications, is notably affected by this demand. In Bulgaria, it serves as a primary source of vegetable fats, ranking second to butter in daily consumption. The aim of this study is to evaluate and propose methods to improve energy and resource efficiency in sunflower oil production in Bulgaria. The analysis is based on data from an energy audit conducted in 2023 at an industrial sunflower oil production facility. Reconstruction and modernization initiatives, which included the installation of high-performance, energy-efficient equipment, led to a 34% increase in energy efficiency. The findings highlight the importance of adjusting the technological parameters such as temperature, pressure, grinding level, and pressing time to reduce energy use and operational costs. Additionally, resource efficiency is improved through more effective raw material utilization and waste reduction. These strategies not only enhance the economic and environmental performance of sunflower oil production but also support sustainable development and competitiveness within the industry. The improvement reduces hexane use by approximately 2%, resulting in energy savings of 12–15 kWh/t of processed seeds and a reduction in CO2 emissions by 3–4 kg/t, thereby improving the environmental profile of sunflower oil production. Full article
(This article belongs to the Special Issue Interdisciplinary Insights in Engineering Research)
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23 pages, 782 KiB  
Article
From Local Actions to Global Impact: Overcoming Hurdles and Showcasing Sustainability Achievements in the Implementation of SDG12
by John N. Hahladakis
Sustainability 2025, 17(15), 7106; https://doi.org/10.3390/su17157106 - 5 Aug 2025
Abstract
This study examines the progress, challenges, and successes in implementing Sustainable Development Goal 12 (SDG12), focusing on responsible consumption and production, using Qatar as a case study. The State has integrated Sustainable Consumption and Production (SCP) into national policies, established coordination mechanisms, and [...] Read more.
This study examines the progress, challenges, and successes in implementing Sustainable Development Goal 12 (SDG12), focusing on responsible consumption and production, using Qatar as a case study. The State has integrated Sustainable Consumption and Production (SCP) into national policies, established coordination mechanisms, and implemented action plans aligned with SDG12 targets. Achievements include renewable energy adoption, waste management reforms, and sustainable public procurement, though challenges persist in rationalizing fossil fuel subsidies, addressing data gaps, and enhancing corporate sustainability reporting. Efforts to reduce food loss and waste through redistribution programs highlight the country’s resilience, despite logistical obstacles. The nation has also advanced hazardous waste management, environmental awareness, and sustainable tourism policies, though gaps in data systems and policy coherence remain. Qatar’s approach provides a valuable local-to-global example of balancing resource-dependent economies with sustainability goals. Its strategies and lessons offer potential adaptability for other nations, especially those facing similar challenges in achieving SDG12. By strengthening data systems, enhancing policy integration, and fostering regional and international cooperation, Qatar’s efforts underscore the importance of aligning economic growth with environmental stewardship, serving as a blueprint for global sustainability initiatives. Full article
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20 pages, 3741 KiB  
Article
Use of Amino Acids and Organic Waste Extracts to Improve the Quality of Liquid Nitrogen–Calcium–Magnesium Fertilizers
by Eglė Didžiulytė and Rasa Šlinkšienė
Sustainability 2025, 17(15), 7081; https://doi.org/10.3390/su17157081 - 5 Aug 2025
Viewed by 61
Abstract
Agriculture is one of the most important sectors of the global economy, but it increasingly faces sustainability challenges in meeting rising food demands. The intensive use of mineral fertilizers not only improves yields, but also causes negative environmental impacts such as increasing greenhouse [...] Read more.
Agriculture is one of the most important sectors of the global economy, but it increasingly faces sustainability challenges in meeting rising food demands. The intensive use of mineral fertilizers not only improves yields, but also causes negative environmental impacts such as increasing greenhouse gas emissions, water eutrophication, and soil degradation. To develop more sustainable solutions, the focus is on organic fertilizers, which are produced using waste and biostimulants such as amino acids. The aim of this study was to develop and characterize liquid nitrogen–calcium–magnesium fertilizers produced by decomposing dolomite with nitric acid followed by further processing and to enrich them with a powdered amino acid concentrate Naturamin-WSP and liquid extracts from digestate, a by-product of biogas production. Nutrient-rich extracts were obtained using water and potassium hydroxide solutions, with the latter proving more effective by yielding a higher organic carbon content (4495 ± 0.52 mg/L) and humic substances, which can improve soil structure. The produced fertilizers demonstrated favourable physical properties, including appropriate viscosity and density, as well as low crystallization temperatures (eutectic points from –3 to –34 °C), which are essential for storage and application in cold climates. These properties were achieved by adjusting the content of nitrogenous compounds and bioactive extracts. The results of the study show that liquid fertilizers enriched with organic matter can be an effective and more environmentally friendly alternative to mineral fertilizers, contributing to the development of the circular economy and sustainable agriculture. Full article
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22 pages, 2666 KiB  
Article
Comparative Proteomic Analysis of Flammulina filiformis Reveals Substrate-Specific Enzymatic Strategies for Lignocellulose Degradation
by Weihang Li, Jiandong Han, Hongyan Xie, Yi Sun, Feng Li, Zhiyuan Gong and Yajie Zou
Horticulturae 2025, 11(8), 912; https://doi.org/10.3390/horticulturae11080912 - 4 Aug 2025
Viewed by 130
Abstract
Flammulina filiformis, one of the most delicious and commercially important mushrooms, demonstrates remarkable adaptability to diverse agricultural wastes. However, it is unclear how different substrates affect the degradation of lignocellulosic biomass and the production of lignocellulolytic enzymes in F. filiformis. In [...] Read more.
Flammulina filiformis, one of the most delicious and commercially important mushrooms, demonstrates remarkable adaptability to diverse agricultural wastes. However, it is unclear how different substrates affect the degradation of lignocellulosic biomass and the production of lignocellulolytic enzymes in F. filiformis. In this study, label-free comparative proteomic analysis of F. filiformis cultivated on sugarcane bagasse, cotton seed shells, corn cobs, and glucose substrates was conducted to identify degradation mechanism across various substrates. Label-free quantitative proteomics identified 1104 proteins. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis of protein expression differences were predominantly enriched in energy metabolism and carbohydrate metabolic pathways. Detailed characterization of carbohydrate-active enzymes among the identified proteins revealed glucanase (GH7, A0A067NSK0) as the key enzyme. F. filiformis secreted higher levels of cellulases and hemicellulases on sugarcane bagasse substrate. In the cotton seed shells substrate, multiple cellulases functioned collaboratively, while in the corn cobs substrate, glucanase predominated among the cellulases. These findings reveal the enzymatic strategies and metabolic flexibility of F. filiformis in lignocellulose utilization, providing novel insights for metabolic engineering applications in biotechnology. The study establishes a theoretical foundation for optimizing biomass conversion and developing innovative substrates using targeted enzyme systems. Full article
(This article belongs to the Special Issue Advances in Propagation and Cultivation of Mushroom)
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10 pages, 1243 KiB  
Proceeding Paper
Innovative Approach to Teaching Sustainable Development at Teacher Training College Through Project for Secondary Use of Recycled Electrical Materials
by Tetjana Tomaskova, Jan Krotky and Jarmila Honzikova
Eng. Proc. 2025, 103(1), 2; https://doi.org/10.3390/engproc2025103002 - 4 Aug 2025
Viewed by 129
Abstract
Higher education is increasingly emphasizing sustainable development due to the growing awareness of environmental issues. Educators must be able to integrate sustainability principles into teaching, inspire students to behave responsibly, and promote environmental protection. In this article, we present an innovative approach through [...] Read more.
Higher education is increasingly emphasizing sustainable development due to the growing awareness of environmental issues. Educators must be able to integrate sustainability principles into teaching, inspire students to behave responsibly, and promote environmental protection. In this article, we present an innovative approach through the secondary use of recycled electronic components to reduce electronic waste and practically teach students about sustainability. The project of making clocks from E-waste included stages from design to testing, providing students with practical skills and emphasizing the importance of recycling in technical education. Full article
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19 pages, 9135 KiB  
Article
A Study on the Characterization of Asphalt Plant Reclaimed Powder Using Fourier Transform Infrared Spectroscopy
by Hao Wu, Daoan Yu, Wentao Wang, Chuanqi Yan, Rui Xiao, Rong Chen, Peng Zhang and Hengji Zhang
Materials 2025, 18(15), 3660; https://doi.org/10.3390/ma18153660 - 4 Aug 2025
Viewed by 176
Abstract
Asphalt plant reclaimed powder is a common solid waste in road engineering. Reusing reclaimed powder as filler holds significant importance for environmental protection and resource conservation. The key factors affecting the feasibility of reclaimed powder reuse are its acidity/alkalinity and cleanliness. Traditional evaluation [...] Read more.
Asphalt plant reclaimed powder is a common solid waste in road engineering. Reusing reclaimed powder as filler holds significant importance for environmental protection and resource conservation. The key factors affecting the feasibility of reclaimed powder reuse are its acidity/alkalinity and cleanliness. Traditional evaluation methods, such as the methylene blue test and plasticity index, can assess reclaimed powder properties to guide its recycling. However, these methods suffer from inefficiency, strong empirical dependence, and high variability. To address these limitations, this study proposes a rapid and precise evaluation method for reclaimed powder properties based on Fourier transform infrared spectroscopy (FTIR). To do so, five field-collected reclaimed powder samples and four artificial samples were evaluated. Scanning electron microscopy (SEM), X-ray fluorescence spectroscopy (XRF), and X-ray diffraction (XRD) were employed to characterize their microphase morphology, chemical composition, and crystal structure, respectively. Subsequently, FTIR was used to establish correlations between key acidity/alkalinity, cleanliness, and multiple characteristic peak intensities. Representative infrared characteristic peaks were selected, and a quantitative functional group index (Is) was proposed to simultaneously evaluate acidity/alkalinity and cleanliness. The results indicate that reclaimed powder primarily consists of tiny, crushed stone particles and dust, with significant variations in crystal structure and chemical composition, including calcium carbonate, silicon oxide, iron oxide, and aluminum oxide. Some samples also contained clay, which critically influenced the reclaimed powder properties. Since both filler acidity/alkalinity and cleanliness are affected by clay (silicon/carbon ratio determining acidity/alkalinity and aluminosilicate content affecting cleanliness), this study calculated four functional group indices based on FTIR absorption peaks, namely the Si-O-Si stretching vibration (1000 cm−1) and the CO32− asymmetric stretching vibration (1400 cm−1). These indices were correlated with conventional testing results (XRF for acidity/alkalinity, methylene blue value, and pull-off strength for cleanliness). The results show that the Is index exhibited strong correlations (R2 = 0.89 with XRF, R2 = 0.80 with methylene blue value, and R2 = 0.96 with pull-off strength), demonstrating its effectiveness in predicting both acidity/alkalinity and cleanliness. The developed method enhances reclaimed powder detection efficiency and facilitates high-value recycling in road engineering applications. Full article
(This article belongs to the Special Issue Innovative Approaches in Asphalt Binder Modification and Performance)
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17 pages, 3816 KiB  
Article
Charging Station Siting and Capacity Determination Based on a Generalized Least-Cost Model of Traffic Distribution
by Mingzhao Ma, Feng Wang, Lirong Xiong, Yuhonghao Wang and Wenxin Li
Algorithms 2025, 18(8), 479; https://doi.org/10.3390/a18080479 - 4 Aug 2025
Viewed by 164
Abstract
With the popularization of electric vehicles and the continuous expansion of the electric vehicle market, the construction and management of charging facilities for electric vehicles have become important issues in research and practice. In some remote areas, the charging stations are idle due [...] Read more.
With the popularization of electric vehicles and the continuous expansion of the electric vehicle market, the construction and management of charging facilities for electric vehicles have become important issues in research and practice. In some remote areas, the charging stations are idle due to low traffic flow, resulting in a waste of resources. Areas with high traffic flow may have fewer charging stations, resulting in long queues and road congestion. The purpose of this study is to optimize the location of charging stations and the number of charging piles in the stations based on the distribution of traffic flow, and to construct a bi-level programming model by analyzing the distribution of traffic flow. The upper-level planning model is the user-balanced flow allocation model, which is solved to obtain the optimal traffic flow allocation of the road network, and the output of the upper-level planning model is used as the input of the lower-layer model. The lower-level planning model is a generalized minimum cost model with driving time, charging waiting time, charging time, and the cost of electricity consumed to reach the destination of the trip as objective functions. In this study, an empirical simulation is conducted on the road network of Hefei City, Anhui Province, utilizing three algorithms—GA, GWO, and PSO—for optimization and sensitivity analysis. The optimized results are compared with the existing charging station deployment scheme in the road network to demonstrate the effectiveness of the proposed methodology. Full article
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22 pages, 2179 KiB  
Article
Conversion of Oil Palm Kernel Shell Wastes into Active Biocarbons by N2 Pyrolysis and CO2 Activation
by Aik Chong Lua
Clean Technol. 2025, 7(3), 66; https://doi.org/10.3390/cleantechnol7030066 - 4 Aug 2025
Viewed by 196
Abstract
Oil palm kernel shell is an abundant agricultural waste generated by the palm oil industry. To achieve sustainable use of this waste, oil palm kernel shells were converted into valuable resources as active biocarbons. A two-stage preparation method involving N2 pyrolysis, followed [...] Read more.
Oil palm kernel shell is an abundant agricultural waste generated by the palm oil industry. To achieve sustainable use of this waste, oil palm kernel shells were converted into valuable resources as active biocarbons. A two-stage preparation method involving N2 pyrolysis, followed by CO2 activation, was used to produce the active biocarbon. The optimum pyrolysis conditions that produced the largest BET surface area of 519.1 m2/g were a temperature of 600 °C, a hold time of 2 h, a nitrogen flow rate of 150 cm3/min, and a heating rate of 10 °C/min. The optimum activation conditions to prepare the active biocarbon with the largest micropore surface area or the best micropore/BET surface area combination were a temperature of 950 °C, a CO2 flow rate of 300 cm3/min, a heating rate of 10 °C/min, and a hold time of 3 h, yielding BET and micropore surface areas of 1232.3 and 941.0 m2/g, respectively, and consisting of 76.36% of micropores for the experimental optimisation technique adopted here. This study underscores the importance of optimising both the pyrolysis and activation conditions to produce an active biocarbon with a maximum micropore surface area for gaseous adsorption applications, especially to capture CO2 greenhouse gas, to mitigate global warming and climate change. Such a comprehensive and detailed study on the conversion of oil palm kernel shell into active biocarbon is lacking in the open literature. The research results provide a practical blueprint on the process parameters and technical know-how for the industrial production of highly microporous active biocarbons prepared from oil palm kernel shells. Full article
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20 pages, 3108 KiB  
Article
Dynamic Expectation–Satisfaction Relationship in Sustainable Experiences with Product: A Comparative Study of Durable Goods, FMCG, and Digital Products
by Zhenhua Wu, Kenta Ono and Yuting Wu
Sustainability 2025, 17(15), 7045; https://doi.org/10.3390/su17157045 - 3 Aug 2025
Viewed by 215
Abstract
This study adopts a dynamic Expectancy–Disconfirmation framework to investigate the evolving nature of user satisfaction across three product categories: durable goods, fast-moving consumer goods (FMCG), and digital products. A 25-day longitudinal experiment involving 128 participants was conducted, during which users engaged with their [...] Read more.
This study adopts a dynamic Expectancy–Disconfirmation framework to investigate the evolving nature of user satisfaction across three product categories: durable goods, fast-moving consumer goods (FMCG), and digital products. A 25-day longitudinal experiment involving 128 participants was conducted, during which users engaged with their most recently purchased products and provided repeated subjective evaluations over time. The findings reveal dynamic changes in the influence of expectations and perceived performance on satisfaction throughout the product usage cycle. For durable goods and FMCG, both expectations and perceived performance gradually declined, accompanied by a weakening effect of expectations on satisfaction. In contrast, digital products exhibited greater volatility, lacking a stable experiential baseline and resulting in greater fluctuations in satisfaction trajectories. Moreover, external contextual and emotional factors were found to play a more significant role in shaping satisfaction with physical products, beyond the scope of the traditional expectancy–performance model. These insights offer theoretical and managerial implications for sustainable product and experience design. In particular, they highlight the importance of implementing experience-stabilizing strategies in digital consumption contexts to support user well-being and enhance continuous product utilization, thereby maximizing product potential and reducing waste. Full article
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16 pages, 1541 KiB  
Article
Economic Dispatch Strategy for Power Grids Considering Waste Heat Utilization in High-Energy-Consuming Enterprises
by Lei Zhou, Ping He, Siru Wang, Cailian Ma, Yiming Zhou, Can Cai and Hongbo Zou
Processes 2025, 13(8), 2450; https://doi.org/10.3390/pr13082450 - 2 Aug 2025
Viewed by 269
Abstract
Under the construction background of carbon peak and carbon neutrality, high-energy-consuming enterprises, represented by the electrolytic aluminum industry, have become important carriers for energy conservation and emission reduction. These enterprises are characterized by significant energy consumption and high carbon emissions, greatly impacting the [...] Read more.
Under the construction background of carbon peak and carbon neutrality, high-energy-consuming enterprises, represented by the electrolytic aluminum industry, have become important carriers for energy conservation and emission reduction. These enterprises are characterized by significant energy consumption and high carbon emissions, greatly impacting the economic and environmental benefits of regional power grids. Existing research often focuses on grid revenue, leaving high-energy-consuming enterprises in a passive regulatory position. To address this, this paper constructs an economic dispatch strategy for power grids that considers waste heat utilization in high-energy-consuming enterprises. A typical representative, electrolytic aluminum load and its waste heat utilization model, for the entire production process of high-energy-consuming loads, is established. Using a tiered carbon trading calculation formula, a low-carbon production scheme for high-energy-consuming enterprises is developed. On the grid side, considering local load levels, the uncertainty of wind power output, and the energy demands of aluminum production, a robust day-ahead economic dispatch model is established. Case analysis based on the modified IEEE-30 node system demonstrates that the proposed method balances economic efficiency and low-carbon performance while reducing the conservatism of traditional optimization approaches. Full article
(This article belongs to the Section Energy Systems)
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8 pages, 405 KiB  
Brief Report
Characterization of DNA Viruses in Hindgut Contents of Protaetia brevitarsis Larvae
by Jean Geung Min, Namkyong Min, Binh T. Nguyen, Rochelle A. Flores and Dongjean Yim
Insects 2025, 16(8), 800; https://doi.org/10.3390/insects16080800 - 1 Aug 2025
Viewed by 264
Abstract
The scarab species Protaetia brevitarsis, an edible insect, has been used in traditional medicine, as animal feed, and for converting agricultural organic wastes into biofertilizer. The intestinal tract, which contains a diverse array of microbiota, including viruses, plays a critical role in [...] Read more.
The scarab species Protaetia brevitarsis, an edible insect, has been used in traditional medicine, as animal feed, and for converting agricultural organic wastes into biofertilizer. The intestinal tract, which contains a diverse array of microbiota, including viruses, plays a critical role in animal health and homeostasis. We previously conducted a comparative analysis of the gut microbiota of third-instar larvae of P. brevitarsis obtained from five different farms and found significant differences in the composition of the gut bacterial microbiota between farms. To better understand the gut microbiota, the composition of DNA viruses in the hindgut contents of P. brevitarsis larvae obtained from five farms was investigated using metagenomic sequencing in this study. The β-diversity was significantly different between metagenomic data obtained from the five farms (PERMANOVA, pseudo-F = 46.95, p = 0.002). Family-based taxonomic analysis indicated that the relative abundance of viruses in the gut overall metagenome varied significantly between farms, with viral reads comprising approximately 41.2%, 15.0%, 4.3%, 4.0%, and 1.6% of metagenomic sequences from the farms Tohamsan gumbengi farm (TO), Secomnalagum gumbengi (IS), Gumbengi brothers (BR), Kyungpook farm (KB), and Jhbio (JH), respectively. More than 98% of the DNA viruses in the hindgut were bacteriophages, mainly belonging to the Siphoviridae family. At the species level, Phage Min1, infecting the genus Microbacterium, was detected in all farms, and it was the most abundant bacteriophage in intestinal microbiota, with a prevalence of 0.9% to 29.09%. The detected eukaryotic DNA viruses accounted for 0.01% to 0.06% of the intestinal microbiota and showed little or no relationship with insect viruses. Therefore, they most likely originated from contaminated feed or soil. These results suggest that the condition of substrates used as feed is more important than genetic factors in shaping the intestinal viral microbiota of P. brevitarsis larvae. These results can be used as reference data for understanding the hindgut microbiota of P. brevitarsis larvae and, more generally, the gut virome of insects. Full article
(This article belongs to the Topic Diversity of Insect-Associated Microorganisms)
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23 pages, 2231 KiB  
Review
Advanced Nuclear Reactors—Challenges Related to the Reprocessing of Spent Nuclear Fuel
by Katarzyna Kiegiel, Tomasz Smoliński and Irena Herdzik-Koniecko
Energies 2025, 18(15), 4080; https://doi.org/10.3390/en18154080 - 1 Aug 2025
Viewed by 319
Abstract
Nuclear energy can help stop climate change by generating large amounts of emission-free electricity. Nuclear reactor designs are continually being developed to be more fuel efficient, safer, easier to construct, and to produce less nuclear waste. The term advanced nuclear reactors refers either [...] Read more.
Nuclear energy can help stop climate change by generating large amounts of emission-free electricity. Nuclear reactor designs are continually being developed to be more fuel efficient, safer, easier to construct, and to produce less nuclear waste. The term advanced nuclear reactors refers either to Generation III+ and Generation IV or small modular reactors. Every reactor is associated with the nuclear fuel cycle that must be economically viable and competitive. An important matter is optimization of fissile materials used in reactor and/or reprocessing of spent fuel and reuse. Currently operating reactors use the open cycle or partially closed cycle. Generation IV reactors are intended to play a significant role in reaching a fully closed cycle. At the same time, we can observe the growing interest in development of small modular reactors worldwide. SMRs can adopt either fuel cycle; they can be flexible depending on their design and fuel type. Spent nuclear fuel management should be an integral part of the development of new reactors. The proper management methods of the radioactive waste and spent fuel should be considered at an early stage of construction. The aim of this paper is to highlight the challenges related to reprocessing of new forms of nuclear fuel. Full article
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