Search Results (354)

Slaughterhouse detection of lesions compatible with bovine tuberculosis represents a key passive surveillance component in Santa Catarina, Brazil, yet subsequent trace-back investigations often fail to identify infected farms. This study developed a quantitative framework to prioritize epidemiological investigations by estimating the probability of infection associated with each farm connected to PCR-confirmed cases. Using official movement records and historical diagnostic data, we reconstructed the lifetime contact networks of slaughtered cattle presenting confirmed Mycobacterium bovis lesions (n = 502). For each sentinel animal–farm interaction (n = 1452), infection probability was estimated through a non-homogeneous Poisson process incorporating exposure duration and the time-weighted average herd size as determinants of infectious pressure. After evaluating stochastic variability through Monte Carlo simulation, a deterministic model using the mean infectious-pressure parameter was applied to classify farms into high-, medium-, and low-risk categories. Model performance was assessed using validated field diagnostic outcomes within a three-year temporal window. High-risk farms represented most validated contacts (58%) and demonstrated a relative risk of 3.48 compared with lower-risk category. These findings indicate that a standardized risk-based classification can substantially improve the prioritization of trace-back investigations, offering a practical decision-support tool to enhance bovine tuberculosis surveillance and contribute to eradication strategies in Santa Catarina. Full article

Livestock manure management contributes substantially to agricultural greenhouse gas emissions, making the adoption of low-carbon approaches urgent in ecologically sensitive regions. This study focuses on the County-wide Livestock Manure Resource Utilization Project in Danjiangkou City, the core water source area of China’s South-to-North Water Diversion Project. Based on field survey data, IPCC Guidelines, and a life cycle assessment framework, this study established a carbon accounting boundary covering excretion, collection, storage, treatment, and utilization stages. A scenario analysis was conducted to compare 2023 baseline emissions with 2026 project emissions and to quantify the carbon reduction potential. The research findings indicate that the overall carbon reduction rate following the project’s implementation reached 40.8%. However, the effectiveness varied considerably across the four management models. The Sedimentation–Crop Model and the Housing–Bedding Integrated Model, which employed integrated systemic interventions, achieved reductions of 61.50% and 60.09%, respectively. In contrast, the “124” Healthy Breeding Model and the Raised-Bedding Composting System, which relied primarily on single-stage upgrades, achieved reductions of only 32.04% and 27.70%. This disparity suggests that in decentralized livestock operations, isolated technological improvements fall short; meaningful decarbonization requires systemic interventions across the entire manure management chain. The findings provide a reference for low-carbon livestock manure management and regional development in ecologically sensitive areas. Full article

Wildlife health surveillance is a vital element of disease prevention, biodiversity conservation, and public health protection, especially as most emerging infectious diseases originate from wildlife. In Slovenia, long-term passive surveillance based on necropsy data has yielded valuable insights into wildlife mortality patterns over the past three decades, despite inherent limitations such as carcass detectability, reporting bias, scavenging, and decomposition. Ongoing cooperation among governmental institutions, veterinary services, hunters, and wildlife management organisations has enabled the effective operation of this system, although passive surveillance remains subject to spatial, temporal, and species-specific biases. Necropsy data show that infectious diseases, particularly parasitic infections, are the main causes of mortality in key species such as roe deer and chamois, reflecting both their population abundance and targeted monitoring. In contrast, carcasses of species such as wild boar, red deer, small mammals, and birds are underrepresented due to ecological factors, biosecurity constraints, or low detectability. Overall, while passive wildlife surveillance does not provide representative population-level mortality estimates, it remains a reliable tool for identifying the presence or absence of significant diseases and for understanding broad mortality patterns when interpreted in the context of known methodological and ecological limitations. Full article

Small ruminant production is vital for rural livelihoods and food security in Saudi Arabia but faces persistent constraints, including high feed costs, limited veterinary access, low reproductive efficiency, and weak extension services. To address these gaps, a Farm Service Delivery Model (FSDM) was piloted, which involved embedding trained livestock technicians into communities to deliver integrated on-farm services. This study evaluated the impact of the FSDM on 47 farms across three regions over 6–12 months. The key results showed significant improvements: flock size increased by 28%, the lambing rate per ewe doubled from 0.39 to 0.80, twin births tripled, mortality declined from 23.8% to 8.0%, and milk production more than doubled. Economic analysis revealed a benefit–cost ratio of 3.02, indicating high return on investment. Scaling the FSDM nationally could generate up to USD 4.8 billion in added meat and milk value over five years while reducing meat imports by 48%. The model aligns with Saudi Vision 2030 and offers a scalable and sustainable pathway to enhance productivity, resilience, and rural income. Policy recommendations include institutional integration, digital innovation, blended financing, and strengthened breeding programs. Full article

The active constituents of essential plant oils (EOAIs), monoterpenoid carvacrol and monoterpene p-cymene, are widely distributed in many aromatic plants and their products. They differ in that carvacrol has a phenolic functional group. The numerous pharmacological effects of these two EOAIs are well known. In different doses/concentrations, they exhibit analgesic, neuroprotective, vasorelaxant, anti-inflammatory, antiviral, antibacterial and antiparasitic effects. The acute toxicity of carvacrol and p-cymene in rats and the free-living nematode Caenorhabditis elegans was investigated. Furthermore, the impact of subacute administration of these two terpenes on general health, CNS integration, i.e., motor coordination and balance of rats, as well as their effects on the movement of adult C. elegans, was also examined. The aim was to compare the effects and describe in more detail the selective toxicity of carvacrol and p-cymene. The calculated LD₅₀ value of carvacrol was 790.15 ± 1.15 mg/kg, while the LD₅₀ value of p-cymene is above 3000 mg/kg. Tested doses of carvacrol and p-cymene administered for 28 days (50, 100, and 200 mg/kg) did not exert any effect on the CNS of rats or cause any clinical disorders. LC₅₀ value of carvacrol for adult C. elegans was 184.13 ± 1.51 μM and for p-cymene 1268 ± 1.65 μM. In subacute testing, carvacrol showed negative effects on C. elegans reproduction, distance traveled, movement speed and rotational index at lower concentrations than p-cymene, indicating higher toxicity, which may be due to its phenolic structure. On the other hand, although less toxic to C. elegans, p-cymene exhibited a specific effect on worm motility, with more rolling which should be further investigated, and can be a consequence of cuticle damage or loss of orientation. Full article

Production diseases in dairy cattle impose economic and welfare burdens, yet few studies quantify costs using on-farm cases. This study aimed to estimate costs and lost revenues at the individual-animal level in 10 German dairy farms (average of 592 cows; 9694 kg marketed milk/cow/year; 32.9% culling rate). Each farm was visited for three weeks; diseased cows and calves were examined by a trained veterinarian. Diagnoses, treatments, labour times, and outcomes were recorded, and costs calculated for labour, products, veterinary and orthopaedic services, discarded milk, decreased milk yield, culling, book loss, and reduced carcass value. In total, 1272 single-animal cases were included: 68% were stand-alone diseases, 11% involved multiple diagnoses within one organ system, and 21% affected several organ systems. When several diseases occurred in the same animal, total costs and lost revenues were greater than the sum of stand-alone cases, indicating compounding effects. High-impact conditions included mastitis, claw disorders, left displaced abomasum, and multimorbidity; per-case losses ranged from €43 (digital dermatitis) to >€1200 (left displaced abomasum with complications). Labour and culling-related costs were higher than reported, and productivity losses exceeded treatment costs in many cases. Findings support farm-level decision-making, prevention, and parameterization of future dynamic models. Full article

This study examined general attitudes toward animals among staff working in licensed animal hospitals in Türkiye. Using the 10-item Animal Attitude Scale (AAS-10), an online survey was administered to animal hospital staff; 193 questionnaires were completed from 17 provinces. Because total scores deviated from normality, group comparisons were conducted using non-parametric tests, and a multiple linear regression model was fitted to examine joint associations with demographic and professional variables. Overall, participants reported generally positive attitudes (mean AAS-10 = 36.7 ± 5.85; range 10–50). Women scored higher than men (p < 0.001), and respondents aged 20–29 years scored higher than those aged ≥40 years (p = 0.029) in unadjusted comparisons; however, the age pattern was small and did not persist after adjustment for gender and other covariates. Professional variables, including occupational role and length of service, were not meaningfully associated with total scores. Exploratory item-level analyses suggested gender-related differences in acceptance of specific forms of animal use, but these should be interpreted cautiously given multiple comparisons. These findings provide a descriptive baseline of AAS-10 scores in a heterogeneous animal hospital workforce and support hypothesis generation for future research that incorporates practice-specific measures. Full article

Background: Raw-meat-based diets (RMBDs) for companion animals have gained popularity but may serve as vehicles for antimicrobial-resistant (AMR) bacteria, posing risks to animal and public health. This study investigated the occurrence and risk factors of AMR in indicator bacteria (Escherichia coli, Enterococcus faecalis, Enterococcus faecium) and Campylobacter spp. from commercial RMBD products. Methods: In 2023, 50 RMBD samples were collected in Belgium, representing 21 brands from five countries. After both selective and non-selective isolation and MALDI-TOF identification, antimicrobial susceptibility testing of the isolates was performed using broth microdilution. Results: From non-selective media, E. coli was found in 45 samples (90.0%), E. faecalis in 31 samples (62.0%), E. faecium in 23 samples (46.0%), and Campylobacter spp. in 3 samples (6.0%). Among these, one E. faecalis strain with acquired resistance to vancomycin and daptomycin was isolated. Multidrug resistance (MDR) was identified in 17 isolates from 15 samples (30.0%), including 14 MDR E. coli, 1 MDR E. faecalis, and 2 MDR E. faecium. From selective media, presumptive ESBL/AmpC-producing E. coli were detected in 17 samples (34.0%), and 5 E. faecium from linezolid-supplemented media were confirmed by the broth microdilution method. Samples from Belgian origin showed significantly higher E. faecium prevalence (76.5%) compared to Dutch samples (21.4%) (OR = 11.9, p < 0.001). Minor livestock sources were associated with increased MDR risk (OR = 5.52, p = 0.016). Conclusions: Commercial RMBDs in Belgium exhibit widespread bacterial contamination with concerning AMR patterns. These findings highlight the need for improved production standards in the RMBD industry and the need to raise awareness in pet owners. Full article

Pasture ecosystems provide essential ecosystem services, yet poisonous plants create persistent veterinary and economic risks. We examined how hydroclimatic variability restructures the poisonous-plant assemblage across three Central Kazakhstan rangelands during an extremely dry year (2023) and an exceptionally wet year (2024). A total of 32 toxic vascular plant species were recorded. Xeromorphic pastures maintained a stable floristic core across years, whereas the wet year triggered recruitment of wet-associated poisonous taxa (hydrophytic/hygrophytic group) exclusively in the Nura River floodplain and increased species richness. Thus, interannual variability was controlled by hydrologically sensitive habitats rather than wholesale community turnover. The principal grazing hazard was associated with flood-related species (e.g., Cicuta virosa, Oenanthe aquatica) and persistent forage contaminants (Datura/Hyoscyamus, Lolium temulentum). These findings indicate that toxic-plant risk follows an asymmetric seasonal pattern: episodic post-flood hazard in floodplains combined with constant background risk in steppe pastures. Therefore, grazing management should integrate event-based monitoring of wet habitats with continuous forage-quality control. Full article

Gilts have a lower capacity for voluntary feed intake and body reserves than multiparous sows, which limits their ability to cope with the needs of gestation and lactation. In this study, a nutritional supplement was formulated to support gilts during the peripartum period. Both control (C, n = 64) and treatment (T, n = 63) groups received standard commercial diets. Group T received 300gr of supplement per gilt and day for the last 35 days of gestation until the fifth day of lactation. This supplement contained calcium (Ca; 4.1%), sodium (Na; 4.0%), lysine (Lys; 1.96%), methionine (Met; 1.32%), vitamin B₁₂ (0.3 mg/kg), choline chloride (600 mg/kg), betaine (475 mg/kg), and L-carnitine (500 mg/kg). Supplementation significantly reduced (p < 0.050) stillbirth rate, neonatal diarrhea, postpartum hypophagia, and both β-hydroxybutyrate (BHBA) and creatinine (CREA) concentrations (effect sizes: 0.240–0.993). Also, supplementation significantly increased (p < 0.050) piglet weight at birth and at 15 days of lactation and maternal backfat thickness at 26 days of lactation (effect sizes: 0.491–0.719). The concentrations of BHBA and CREA showed significant and negative associations with several productive parameters (p < 0.05); the strength of the associations was low–medium. Targeted peripartum supplementation represents a feasible nutritional strategy for commercial herds characterized by large litter sizes and limited voluntary feed intake capacity. Full article

Unacceptable elimination is a common and frustrating feline behavioural complaint and, when expressed as a repetitive and rigid ritual, it may resemble a pattern of compulsive-like elimination. This retrospective case series reviewed medical records from a single veterinary behaviour service (July 2020–July 2025) to describe cats with longstanding unacceptable urination showing caregiver-reported compulsive features (repetitiveness, redundancy, and rigidity) and to compare outcomes between behavioural modification alone (group B) and behavioural modification plus clomipramine (group C). Twenty-one cats met the inclusion criteria (C, n = 11; B, n = 10) after relevant medical causes had been excluded via physical examination and laboratory testing. Baseline characteristics were comparable between groups, including age, sex distribution, and interval between onset of clinical signs and initiation of therapy. Clinical outcome and time to recovery differed markedly: a positive outcome was recorded in 1/10 cats in group B versus 10/11 in group C after 30 days of therapy (first follow-up). Follow-up completion was also consistently higher in group C across all time points. These findings might suggest a pattern of compulsive-like elimination, with the observed response to clomipramine providing further support for this conceptual framework. Full article

The gut–mammary axis represents a promising therapeutic target for mastitis. Although plant-derived polysaccharides exhibit immunomodulatory properties, their capacity to modulate this axis—and specifically to ameliorate dysbiosis-induced mastitis—remains unexplored. Here, we investigated the therapeutic potential of Taraxacum kok-saghyz leaf-derived polysaccharides (TKP-L) against mastitis in a murine model of gut dysbiosis, with dysbiosis induced by fecal microbiota transplantation (FMT) from donor cows. Pregnant mice (n = 60) with antibiotic-depleted microbiota received FMT suspensions prepared from the feces of healthy dairy cows or cows with clinical mastitis (based on somatic cell count). Mice were randomly divided into five groups: Control (vehicle), M-FMT (mastitis-cow FMT, disease model), H-FMT (healthy-cow FMT), TKP-L (M-FMT + oral TKP-L, 500 mg/kg/day), and Ciprofloxacin (M-FMT + ciprofloxacin, positive Control). After FMT establishment, TKP-L or ciprofloxacin was administered for 14 days. We assessed histopathology, pro-inflammatory mediators (IL-6, IL-1β, TNF-α, MPO), tight junction proteins (occludin, ZO-1, Claudin-3), and bacterial translocation using GFP-E. coli, and gut/milk microbiota via 16S rRNA sequencing. Compared to the M-FMT group, TKP-L treatment significantly alleviated mammary inflammation and pathology, inhibited pro-inflammatory cytokine expression, and enhanced the expression of tight junction proteins in both intestinal and mammary tissues, correlating with reduced bacterial translocation to the mammary gland. Microbiota analysis showed that TKP-L restored microbial homeostasis in the gut and milk, concurrently increasing the relative abundance of beneficial bacteria such as Limosilactobacillus. TKP-L alleviates gut dysbiosis-induced mastitis in mice by concurrently modulating the gut–mammary axis through microbial remodeling, enhancement of epithelial barriers, and anti-inflammatory actions. These findings highlight TKP-L as a promising gut microbiota-targeting candidate for mastitis intervention. Full article

Rabies remains a zoonotic public health problem in Ghana despite the availability of effective preventive measures, including mass dog vaccination and timely post-exposure prophylaxis (PEP). We conducted a community-based cross-sectional mixed-methods study between June and December 2025 to assess rabies-related knowledge, risk perception, health-seeking behaviour following dog bites, and dog vaccination practices within a One Health framework. Structured face-to-face interviews were conducted with 450 adults from selected urban and rural communities in the Greater Accra, Ashanti, and Bono East regions, supplemented by focus group discussions. Quantitative data were analysed using descriptive statistics, chi-square tests, and multivariable logistic regression. Overall, 68% of respondents had heard of rabies; however, detailed knowledge of transmission and prevention was limited, with 189 (42.0%) correctly identifying dogs as the main source of transmission. Following suspected exposure, 162 (36.0%) reported using home remedies or traditional treatments. Dog vaccination coverage was 31.1%, below the level required to interrupt transmission. Educational level, place of residence, and prior dog-bite exposure were significantly associated with rabies knowledge, health-seeking behaviour, and vaccination practices (p < 0.05). This study provides updated evidence on community rabies knowledge, risk perception, and preventive practices, highlighting behavioural and structural gaps that may hinder effective control in Ghana. Full article

Professionalism is central to veterinary practice, shaping not only the quality of care provided to animals but also the wellbeing of practitioners, the satisfaction of clients, and the sustainability of the profession. Prior research has catalogued various attributes of professionalism that are important for career success, but few studies have integrated these multiple perspectives into a cohesive framework. This study synthesizes insights from three key veterinary stakeholder groups—students, clinical practitioners, and clients—using a multi-methods approach including surveys, focus groups, critical incident interviews, and client complaint analyses. Across the datasets, ranking of Likert-scale responses and thematic analysis revealed four recurring themes that were identified as essential for career success: ‘Effective communication’; ‘Accountability, integrity, trustworthiness, and honesty’; ‘Personal wellbeing’; and ‘Quality of service’. These themes were organized into a unifying theoretical model of veterinary professionalism, conceptualized as a ‘navigational compass’, comprising three domains of care: patient-centered care, relationship-centered care, and self-care. By conceptualizing professionalism in terms of a compass, the model illustrates how veterinarians can draw on key professionalism attributes, coupled with consideration of the three domains of veterinary care, to navigate the challenges of practice and sustain long-term career success. The compass provides a reflective framework to guide veterinarians and educators, to support the integration of professionalism into curricula and to guide careers toward excellence in care and lasting personal fulfilment. Full article

The prevention of trichinellosis is one of the most important tasks of the public veterinary services. In most EU countries, mandatory testing for parasites of the genus Trichinella includes domestic pigs as well as game species such as wild boar and bears. In Croatia, hunters occasionally also submit badger (Meles meles) meat for Trichinella testing. In addition to the samples from the genus Meles, samples from two other genera within the same family, Martes and Mustela, were analysed. These specimens were most frequently delivered to the laboratory, as roadkill, within a passive surveillance program for the rabies virus. During a 14-year study period, muscle samples from 40 badgers (M. meles), 11 martens (M. foina) and from one polecat (M. putorius) were examined using the artificial digestion method. Trichinella infection was confirmed in four badgers (10%) and one marten (9.1%). Molecular identification by multiplex PCR revealed T. spiralis and T. britovi in two badger specimens, while T. pseudospiralis was identified in a stone marten. Further genetic analysis of the T. pseudospiralis isolate demonstrated its belonging to the Palaearctic population. This study evaluates the epidemiological significance of these findings in relation to trichinellosis, the most important foodborne parasitosis in Croatia, and emphasizes the need for further epidemiological studies in the country targeting both Mustelidae and T. pseudospiralis in the sylvatic cycle of Trichinella. Full article