Search Results (193)

Oropouche fever (OF), caused by Oropouche virus (OROV), has expanded beyond its Amazonian range into Minas Gerais (MG), Brazil, raising concern about transmission in extra-Amazonian Atlantic Forest landscapes. Critical gaps persist regarding Culicoides vector communities, anthropophily, and climate-sensitive transmission risk in these newly affected regions. We conducted targeted entomological surveys outbreak-driven by human OF cases, standardized across five MG communities using CDC light traps and Protected Human Attraction (PHA) to characterize Culicoides composition. Females of Culicoides underwent RT-qPCR for OROV (n = 819) and physiological assessment (n = 312). We developed an entomological alert framework that integrates blood-fed abundance, minimum infection rate (MIR) upper confidence bounds, and environmental drivers (i.e., mean temperature, relative humidity and precipitation) via generalized additive mixed models, which explained 68% of the variability in Culicoides abundance and the alert index across communities. We collected 1171 Culicoides individuals representing five species (C. leopoldoi, C. paraensis, C. pusillus, C. foxi, and C. limai). C. leopoldoi (79.1%) and C. paraensis (20.3%) were the predominant species; notably, C. paraensis is recognized as the primary vector of OROV in the Americas. C. paraensis was documented for the first time in all five outbreak areas and dominated PHA captures (90%), suggesting anthropophily. Although no specimens tested OROV-positive (consistent with expected field infection rates of 0.01–1%), MIR upper bounds reached 132/1000 in low-sample settings and humidity and temperature strongly modulated abundance. This operational baseline and alert index transform virologically negative, sparse surveillance data into prioritized targets for intensified sampling and vector control during early, low-prevalence phases, when containment of OROV’s extra-Amazonian spread is still achievable. Full article

Natural hydrogen has gained attention as a low-carbon energy vector, and some reported surface expressions have been linked to subcircular patterns, or fairy circles (FC), that may be detectable in multispectral satellite imagery. The Carolina Bays region, on the eastern coast of the United States, was selected because it hosts abundant, well-mapped subcircular depressions. This study aims to comparatively evaluate machine learning algorithms for identifying subcircular structures using Landsat-8 data. We processed 105 Collection 2 Level 2 scenes, masking clouds and shadows using the Level 2 quality band. Pixel-level labels were determined using a well-curated public dataset, derived from a high-resolution LiDAR survey. Traditional models (logistic regression, random forest, and multilayer perceptron) achieved precision scores below 0.66 and enabled a variable-importance analysis, which identified Band 3 (green), Band 6 (SWIR1), and five Normalised Unit Indices as the most predictive features. Deep learning models improved detection, and a U-Net architecture allowed for pixel-level segmentation of FC-like structures, producing false positives mostly in cloudy or shadowed areas. Overall, the results suggest that FC detection from multispectral data alone remains challenging due to class overlap and cloud/shadow contamination. Future work could explore integrating additional non-spectral descriptors, such as morphometric variables, to reduce ambiguities. Full article

The nitrogen-to-phosphorus ratio (TN:TP) is a key indicator influencing phytoplankton nutrient limitation and growth dynamics, directly regulating algal growth rates, abundance, and community structure, thereby affecting the process of water eutrophication. This study aims to evaluate the modeling performance of integrated machine learning approaches for lake total nitrogen to total phosphorus ratios (TN:TP), utilizing Zhuhai-1 hyperspectral satellite imagery to develop a CNN-SVR ensemble model integrating convolutional neural networks and support vector regression for remote sensing inversion of lake TN:TP ratios. Performance is evaluated against random forest (RF) and convolutional neural network (CNN) models, systematically analyzing spatial distribution patterns and primary drivers. Results indicate that the CNN-SVR model demonstrated superior performance among the tested models, with R², RMSE, MAPD, and RPD values of 0.856, 2.675, 9.516%, and 2.390, respectively. Spatially, the nitrogen-to-phosphorus ratio in lakes during the growing season exhibits an increasing trend from the western to the eastern half of the lake, progressing from northwest to southeast. When TN:TP falls below 9, algal growth becomes nitrogen-limited, indicating a higher degree of eutrophication; when TN:TP exceeds 22.6, phosphorus becomes the limiting factor, indicating lower eutrophication levels. A similar distribution pattern is observed during the non-growing season. Regarding driving mechanisms, the nitrogen-to-phosphorus ratio during the growing season is primarily influenced by TN accumulation and shows significant correlations with dissolved oxygen (DO) and pH. During the non-growing season, while still affected by TN input, its association with other water quality parameters is weaker. The results indicate that the combined use of CNN and SVR improves feature extraction and model fitting in nitrogen-to-phosphorus ratio inversion and helps clarify its ecological significance as an indicator of algal growth. This provides methodologies and evidence for precise diagnosis and ecological management of lake eutrophication. Full article

Horse flies (Tabanidae) negatively affect livestock by reducing productivity, compromising animal welfare, and serving as mechanical vectors of pathogens. However, the spatial processes shaping their community organization in southern Brazil’s Coastal Plain of Rio Grande do Sul (CPRS) remain poorly understood. To address this, we conducted standardized Malaise-trap surveys and combined them with historical–contemporary comparisons to examine distance–decay patterns in community composition. We evaluated both abundance-based (Bray–Curtis) and presence–absence (Jaccard) dissimilarities using candidate models. Across sites, Tabanus triangulum emerged as the dominant species. Dissimilarity in community structure increased monotonically with geographic distance, with no evidence of abrupt thresholds. The square-root model provided the best fit for abundance-based data, whereas a linear model best described presence–absence patterns, reflecting dispersal limitation and environmental filtering across a heterogeneous coastal landscape. Sites within riparian forests and conservation units displayed higher diversity, emphasizing the ecological role of protected habitats and the importance of maintaining connected corridors. Collectively, these findings establish a process-based framework for surveillance and landscape management strategies to mitigate vector, host contact. Future directions include integrating remote sensing and host distribution, applying predictive validation across temporal scales. Full article

Accurate global mapping of lunar iron oxide (FeO) abundance is essential for understanding the Moon’s geological evolution and for supporting future in situ resource utilization (ISRU). While hyperspectral data from the Moon Mineralogy Mapper (M3) provide a unique combination of high spectral dimensionality, hectometre-scale spatial resolution, and near-global coverage, existing FeO retrieval approaches struggle to fully exploit the high dimensionality, nonlinear spectral variability, and planetary-scale volume of the Global Mode dataset. To address these limitations, we present an integrated machine learning pipeline for estimating lunar FeO abundance from M3 hyperspectral observations. Unlike traditional methods based on raw reflectance or empirical spectral indices, the proposed framework combines Discrete Wavelet Transform (DWT), deep autoencoder-based feature compression, and ensemble regression to achieve robust and scalable FeO prediction. M3 spectra (83 bands, 475–3000 nm) are transformed using a Daubechies-4 (db4) DWT to extract 42 representative coefficients per pixel, capturing the dominant spectral information while filtering high-frequency noise. These features are further compressed into a six-dimensional latent space via a deep autoencoder and used as input to a Random Forest regressor, which outperforms kernel-based and linear Support Vector Regression (SVR) as well as Lasso regression in predictive accuracy and stability. The proposed model achieves an average prediction error of 1.204 wt.% FeO and demonstrates consistent performance across diverse lunar geological units. Applied to 806 orbital tracks (approximately $3.5\times {10}^9$ pixels), covering more than 95% of the lunar surface, the pipeline produces a global FeO abundance map at 150 m per pixel resolution. These results demonstrate the potential of integrating multiscale wavelet representations with nonlinear feature learning to enable large-scale, geochemically constrained planetary mineral mapping. Full article

Social media has the potential to serve beneficial purposes. The abundance of uploaded content and responses from the public generates various opinions, allowing them to be identified as positive or negative regarding the portrayal of Bandung Regency. This research aims to analyse the classification and frequency of words for each sentiment expressed by X (Twitter) users regarding Bandung Regency. The research employs the Support Vector Machine (SVM) method. We expect the results to aid in formulating governmental programmes for Bandung Regency. The research revealed that the SVM model, which uses the Sigmoid kernel function with parameters $C$ = 10 and gamma ($\gamma$) = 1, is the most optimal sentiment classification model for handling an imbalanced dataset. This model achieved an 83.01% negative recall value. Furthermore, frequent words appearing in both classes indicate that several positive opinions about Bandung Regency exhibit similar dominance, except for football dominance in negative opinions. This research pertains to the United Nations Sustainable Development Goals (SDGs), particularly SDG 11 (Sustainable Cities and Communities) and SDG 16 (Peace, Justice, and Strong Institutions). The suggested technique facilitates evidence-based policy reviews, transparent governance, and enhanced responsive public services by analysing public sentiment regarding local government performance. The results illustrate how social media analytics can aid local governments in assessing popular sentiment and pinpointing areas for policy response. Full article

This study employed machine learning to investigate the mechanical behavior of one-part geopolymer (OPG)-stabilized soil subjected to acid erosion. Based on the unconfined compressive strength (UCS) data of acid-eroded OPG-stabilized soil, eight machine learning models, namely, Adaptive Boosting (AdaBoost), Decision Tree (DT), Extra Trees (ET), Gradient Boosting (GB), Light Gradient Boosting Machine (LightGBM), Random Forest (RF), Support Vector Machine (SVM), and eXtreme Gradient Boosting (XGBoost), along with hyper-parameter optimization by Genetic Algorithm (GA), were used to predict the degradation of the UCS of OPG-stabilized soils under different durations of acid erosion. The results showed that GA-SVM (R² = 0.9960, MAE = 0.0289) and GA-XGBoost (R² = 0.9961, MAE = 0.0282) achieved the highest prediction accuracy. SHAP analysis further revealed that solution pH was the dominant factor influencing UCS, followed by the FA/GGBFS ratio, acid-erosion duration, and finally, acid type. The 2D PDP combined with SEM images showed that the microstructure of samples eroded by HNO₃ was marginally denser than that of samples eroded by H₂SO₄, yielding a slightly higher UCS. At an FA/GGBFS ratio of 0.25, abundant silica and hydration products formed a dense matrix and markedly improved acid resistance. Further increases in FA content reduced hydration products and caused a sharp drop in UCS. Extending the erosion period from 0 to 120 days and decreasing the pH from 4 to 2 enlarged the pore network and diminished hydration products, resulting in the greatest UCS reduction. The results of the study provide a new idea for applying the ML model in geoengineering to predict the UCS performance of geopolymer-stabilized soils under acidic erosion. Full article

Bioaerosols emitted from wastewater treatment plants (WWTPs) are key vectors for airborne microbial transmission, yet the mechanisms by which abundant and rare microbial taxa regulate pathogen dynamics remain unclear. This study explored the ecological roles of abundant and rare taxa through a comprehensive analysis of bioaerosols from two full-scale WWTPs, integrating high-throughput sequencing of bacterial and fungal communities. Results showed that the rare taxa exhibited higher alpha diversity, and their community construction was dominated by deterministic processes. While the abundant taxa showed higher spatial homogeneity, and their distribution was more consistent with the neutral model, suggesting the dominance of stochastic processes. Network analysis revealed that rare taxa held keystone topological roles within the microbial networks. Moreover, partial least squares path model quantified their direct effects on pathogen abundance, revealing a strong positive direct effect of abundant bacterial taxa but a significant negative direct effect of rare bacterial taxa. This study elucidates the dual roles of taxa with different abundance levels in community assembly and pathogen regulation, emphasizing that effective risk assessment and management strategies should account not only for the carrier role of abundant taxa but also for the regulatory function of the rare biosphere in shaping pathogen dynamics. Full article

In contemporary education, learners face the challenge of navigating an overwhelming abundance of information. Traditional search methods, often limited to keyword matching, fail to capture the nuanced meaning and relationships within educational materials. Our multimodal approach combines Sentence Transformer for text and Inception V3 for images to generate vector embeddings for textbooks which are stored in an Elasticsearch database. Learners’ queries again are converted to vector embeddings which are matched through cosine similarity with stored embeddings, resulting in retrieval of relevant material which is ranked and then synthesized using large language model (LLM) APIs. The approach retrieves answers based on semantic search rather than keywords. The system also integrates GenAI capabilities separately, specifically leveraging LLM APIs, to generate context-aware answers to user-posed questions at varying levels of complexity, e.g., beginner, intermediate, and advanced. Through comprehensive evaluation, we demonstrate the system’s ability to retrieve coherent answers across multiple sources, offering significant advancements in cross-text and cross-modal retrieval tasks. This work also contributes to the international discourse on ethical GenAI integration in curricula and fosters a collaborative human–AI learning ecosystem. Full article

A compartmental, deterministic model was used to explore the effects of temperature dependency in mosquito mortality on the likelihood of epidemics and the size of outbreaks of Chikungunya virus under Florida temperature conditions. Two known vectors, Aedes albopictus and Ae. aegypti, were included, with similar structure but allowing mortality and abundance parameters to vary between them. The mortality relationship with temperature had a central optimal survival region, with increasing mortality outside these regions. The central temperature and the annual mean temperature were most influential in the likelihood of an epidemic, although the variance explained was low. The central temperature, annual mean temperature and day of virus infection influenced the size of the outbreaks. Regression models including two-way interactions explained more of the variance in outcomes than the main effects models, but there was still substantial variance left unexplained. Given the model structure, higher order interactions would be required to explain most of the variance. Full article

West Nile Virus (WNV) is an emerging arboviral threat in Europe, with rising incidence in Spain since 2004. In 2024, Spain experienced its largest outbreak, primarily in small urban areas of south-western regions. We report a subset of an emergency integrated vector management program, focusing on six municipalities accounting for one-third of all human WNV cases nationwide. Over four months, 725 potential larval sites were inspected during 4026 visits. Adult mosquitoes (n = 2553) were collected with suction traps, and immature stages (n = 4457) with dipper techniques, yielding 11 species. Culex pipiens s.l. was predominant, while Cx. perexiguus, though less abundant, was epidemiologically significant. Cytochrome Oxidase I (COI) gene phylogenetic analysis confirmed Cx. perexiguus, forming a distinct clade from Cx. univittatus. Immature mosquitoes were found in 18.6% of sites, especially irrigation canals, ditches, and backwaters near urban areas. Habitat differences in larval abundance were analyzed using generalized linear mixed models. Targeted larviciding with Bacillus thuringiensis var. israelensis (Bti) and focal adulticiding with cypermethrin totaled 259 interventions (70.4% larviciding, 29.6% adulticiding). A significant 63.9% reduction in larval abundance was observed after five consecutive Bti treatments, with some variation among treatment cycles (52.2–75.5%). Adult activity persisted into late autumn. This study provides the first comprehensive characterization of larval mosquitoes in Spain’s main WNV hotspot, highlighting the need for rapid, coordinated expert interventions and extended seasonal control to prevent future outbreaks. Full article

In response to the limitations of traditional single-signal approaches, which fail to comprehensively reflect fault conditions, and the difficulties of existing feature extraction methods in capturing subtle fault patterns in transformer fault diagnosis, this paper proposes an innovative fault diagnosis methodology. Initially, to address common severe faults in excitation transformers, Principal Component Analysis (PCA) is applied to reduce the dimensionality of multi-source feature data, effectively eliminating redundant information. Subsequently, to mitigate the impact of non-stationary noise interference in voiceprint signals, a Deep Belief Network (DBN) optimized using the Hunter–Prey Optimization (HPO) algorithm is employed to automatically extract deep features highly correlated with faults, thus enabling the detection of complex, subtle fault patterns. For temperature and electrical parameter signals, which contain abundant time-domain information, the Random Forest algorithm is utilized to evaluate and select the most relevant time-domain statistics. Nonlinear dimensionality reduction is then performed using an autoencoder to further reduce redundant features. Finally, a multi-classifier model based on Adaptive Boosting with Support Vector Machine (Adaboost-SVM) is constructed to fuse multi-source heterogeneous information. By incorporating a pseudo-label self-training strategy and integrating a working condition awareness mechanism, the model effectively analyzes feature distribution differences across varying operational conditions, selecting potential unseen condition samples for training. This approach enhances the model’s adaptability and stability, enabling real-time fault diagnosis. Experimental results demonstrate that the proposed method achieves an overall accuracy of 96.89% in excitation transformer fault diagnosis, outperforming traditional models such as SVM, Extreme Gradient Boosting with Support Vector Machine (XGBoost-SVM), and Convolutional Neural Network (CNN). The method proves to be highly practical and generalizable, significantly improving fault diagnosis accuracy. Full article

After water gushing occurs in a coal mine roadway, abundant aggregate needs to be perfused into the water gush roadway to establish a water interception section and reduce the current velocity. Clarifying the water-intercepting aggregate motion path and quantitatively calculating the displacement distance are critical for determining perfusion hole spacing. This paper employs the CFD-DEM coupling approach, which is capable of accurately characterizing the water gush continuous flow properties and the aggregate discrete motion behavior. This can be used to simulate and analyze the water-intercepting aggregate motion in a water gush roadway, categorizing it into three phases: free fall, curvilinear projectile, and sliding. The theoretical motion model aggregate can be developed, and the calculation formulas for aggregate motion distances in each phase derived. A parameter test scheme was designed and combined with numerical simulation methods to verify the accuracy of the formulas. Finally, based on this research, it is proposed that the theoretical model can be used to dynamically optimize the design of perfusion hole spacing, maximizing the synergistic effect of multi-hole perfusion. The selection of aggregate density and size should ensure the vector sum of the aggregate motion distance in phase II and III approaches zero, thereby improving the water-intercepting efficiency. Full article

A rapid targeted screening method for 22 compounds, including flavonoids, glycosides, and phenolics, in Dendrobium officinale was developed using UHPLC–MS/MS, demonstrating good linear correlation coefficients, precision, repeatability, and stability. D. officinale from the Guangnan and Maguan regions can be effectively classified into two distinct categories using PCA. In addition, OPLS-DA discriminant analysis enables clear separation between groups, with samples forming well-defined clusters. The 22 chemical components provide valuable origin-related information for D. officinale. The compounds with VIP values of >1 included eriodictyol, vanillic acid, protocatechuic acid, gentisic acid, and naringenin. The difference in naringenin content between D. officinale from the two production areas was minimal. By contrast, eriodictyol and vanillic acid were relatively abundant in D. officinale from Guangnan, while gentisic acid and protocatechuic acid were more prevalent in D. officinale from Maguan. The pathways with higher Kyoto Encyclopedia of Genes and Genomes enrichment were primarily associated with lipid metabolism and atherosclerosis, fluid shear stress and atherosclerosis, and nonalcoholic fatty liver disease. These findings suggest that D. officinale exhibits promising lipid-balancing properties and potential cardiovascular health benefits. Seven machine learning algorithms—Random Forest, XGBoost, Support Vector Machine, k-Nearest Neighbor, Backpropagation Neural Network, Random Tree, and CatBoost—demonstrated superior accuracy and precision in distinguishing D. officinale from the Guangnan and Maguan regions. The key compounds with higher weights—vanillic acid, chrysoeriol, trigonelline, isoquercitrin, gallic acid, 4-hydroxybenzaldehyde, eriodictyol, sweroside, apigenin, and homoeriodictyol—play a crucial role in model construction and the identification of D. officinale from the Guangnan and Maguan regions. The quantification of 22 compounds using UHPLC–MS/MS, combined with PCA, OPLS-DA, and machine learning, enables effective discrimination of D. officinale from these two Yunnan production areas. Full article

Background/Objectives: Cellular senescence is characterised by irreversible cell cycle arrest and the secretion of a proinflammatory phenotype. In recent years, senescent cell accumulation and senescence-associated secretory phenotype (SASP) secretion have been linked to the onset of chronic degenerative diseases associated with ageing. In this context, the senotherapeutic compounds have emerged as promising drugs that specifically eliminate senescent cells (senolytics) or diminish the damage caused by SASP (senomorphics). On the other hand, computational approaches, such as network pharmacology and machine learning, have revolutionised the identification of novel drugs. These tools enable the analysis of large volumes of compounds and the optimisation of the search for the most promising ones as potential drugs. Therefore, we employed such approaches in the present study to identify potential senotherapeutic compounds. Methods: First, we constructed drug-protein interaction networks related to cellular senescence. Then, using three machine learning models (Random Forest, Support Vector Machine, and K-Nearest Neighbours), we classified these compounds based on their therapeutic potential against senescence. Results: Our results enabled us to identify 714 compounds with potential senescent therapeutic activity, of which 270 exhibited desirable medicinal chemistry properties, and we developed an interactive web tool freely accessible to the scientific community. Conclusions: we found that flavonoids were the most abundant compound class from which 18 have never been reported as senotherapeutics. Full article