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Insects
Special Issues
Challenges in Mosquito Surveillance and Control
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Challenges in Mosquito Surveillance and Control

A special issue of Insects (ISSN 2075-4450).

Deadline for manuscript submissions: 31 July 2026 | Viewed by 1471

Special Issue Editor

Dr. Solomon Birhanie

E-Mail Website
Guest Editor
West Valley Mosquito and Vector Control District, Ontario, CA, USA
Interests: invasive mosquitoes; mosquito control; vector-borne diseases; sterile insect technique; IPM
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Mosquito-borne diseases continue to pose significant threats to global public health, yet the fight against vectors like Aedes, Anopheles, and Culex is increasingly complicated by ecological shifts, insecticide resistance, limited surveillance capacity, and urban expansion. We invite researchers, public health professionals, vector control specialists, and policymakers to submit manuscripts that explore the persistent and emerging challenges in mosquito surveillance and control.

We welcome submissions covering (but not limited to) the following topics:

  • Gaps in entomological surveillance systems;
  • Innovations in mosquito monitoring tools and technologies;
  • Insecticide resistance trends and management strategies;
  • Operational barriers in integrated vector control programs;
  • Data-driven decision-making in low-resource settings;
  • Community engagement and risk communication in control campaigns;
  • Climate and environmental influences on vector dynamics.

Submit your manuscript to contribute to this Special Issue and share lessons learned, novel approaches, and collaborative solutions to strengthen mosquito control efforts worldwide. 

Dr. Solomon Birhanie
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Insects is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • mosquito
  • surveillance
  • control
  • insecticide resistance
  • vectors
  • monitoring

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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Research

17 pages, 2417 KB  
Article
Rapid-Response Vector Surveillance and Emergency Control During the Largest West Nile Virus Outbreak in Southern Spain
by Mikel Alexander González, Carlos Barceló, Roberto Muriel, Juan Jesús Rodríguez, Eduardo Rodríguez, Jordi Figuerola and Daniel Bravo-Barriga
Insects 2025, 16(11), 1100; https://doi.org/10.3390/insects16111100 - 29 Oct 2025
Viewed by 405
Abstract
West Nile Virus (WNV) is an emerging arboviral threat in Europe, with rising incidence in Spain since 2004. In 2024, Spain experienced its largest outbreak, primarily in small urban areas of south-western regions. We report a subset of an emergency integrated vector management [...] Read more.
West Nile Virus (WNV) is an emerging arboviral threat in Europe, with rising incidence in Spain since 2004. In 2024, Spain experienced its largest outbreak, primarily in small urban areas of south-western regions. We report a subset of an emergency integrated vector management program, focusing on six municipalities accounting for one-third of all human WNV cases nationwide. Over four months, 725 potential larval sites were inspected during 4026 visits. Adult mosquitoes (n = 2553) were collected with suction traps, and immature stages (n = 4457) with dipper techniques, yielding 11 species. Culex pipiens s.l. was predominant, while Cx. perexiguus, though less abundant, was epidemiologically significant. Cytochrome Oxidase I (COI) gene phylogenetic analysis confirmed Cx. perexiguus, forming a distinct clade from Cx. univittatus. Immature mosquitoes were found in 18.6% of sites, especially irrigation canals, ditches, and backwaters near urban areas. Habitat differences in larval abundance were analyzed using generalized linear mixed models. Targeted larviciding with Bacillus thuringiensis var. israelensis (Bti) and focal adulticiding with cypermethrin totaled 259 interventions (70.4% larviciding, 29.6% adulticiding). A significant 63.9% reduction in larval abundance was observed after five consecutive Bti treatments, with some variation among treatment cycles (52.2–75.5%). Adult activity persisted into late autumn. This study provides the first comprehensive characterization of larval mosquitoes in Spain’s main WNV hotspot, highlighting the need for rapid, coordinated expert interventions and extended seasonal control to prevent future outbreaks. Full article
(This article belongs to the Special Issue Challenges in Mosquito Surveillance and Control)
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13 pages, 1455 KB  
Article
Effect of Hydrogen Peroxide on Oviposition Site Preference and Egg Hatching of the Aedes aegypti (Linnaeus) Mosquito
by Luka Ndungu, Donald Roberts, Lewis Long, Emilie Goguet, Alex Stubner, Sean Beeman, Stephen Lewandowski and Bernard Okech
Insects 2025, 16(9), 928; https://doi.org/10.3390/insects16090928 - 4 Sep 2025
Viewed by 697
Abstract
Hydrogen peroxide (H2O2) occurs in the environment, including in aquatic environments where mosquitoes might lay eggs. However, little is known about the compound’s impact on mosquitoes. We conducted an experiment to determine the effect of H2O2 [...] Read more.
Hydrogen peroxide (H2O2) occurs in the environment, including in aquatic environments where mosquitoes might lay eggs. However, little is known about the compound’s impact on mosquitoes. We conducted an experiment to determine the effect of H2O2 on Ae. aegypti oviposition behavior and egg hatching using H2O2 concentrations similar to those in natural aquatic environments. Oviposition behavior was evaluated by dual-choice and multi-choice bioassays. Gravid Ae. aegypti mosquitoes were placed in cages with containers with different H2O2 concentrations (5, 25, 50, and 100 μM). After 72 h, the number of eggs laid was compared between oviposition sites with and without H2O2. Additionally, egg hatching was assessed under long-period exposure (48 h) and short-period exposure (2, 4, and 6 h and then in deionized water for up to 48 h). Results showed no significant difference in oviposition preference scores in the multi-choice assay (OAI = −0.135 ± 0.06) (p = 0.138), but a significant difference in the dual-choice assay (0.195 ± 0.01) (p = 0.001). Long-period exposure to H2O2 did not significantly affect hatch rates (11.34%) (p = 0.363), but short-period exposure significantly impacted hatch rates (17%) (p = 0.0001), with period of exposure alone playing a significant role (p < 0.0044). Eggs exposed to 100 μM H2O2 for 2 h (p = 0.0070) and 4 h (p = 0.0036) had significantly higher hatch rates compared to the control. This study demonstrates that low concentrations of H2O2 can influence oviposition site characteristics and egg hatch rates. Combined with other environmental factors, H2O2 can shape the reproductive success of Ae. aegypti, offering potential strategies for mosquito control. Full article
(This article belongs to the Special Issue Challenges in Mosquito Surveillance and Control)
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