Search Results (5,389)

Sequential twin-tunnel excavation has become increasingly common as urban rail networks expand, making both deformation control and construction-phase carbon management essential for sustainable underground development. This study investigates the spatiotemporal development of ground settlement induced by parallel Earth Pressure Balance shield tunnelling in a twin-tunnel section of the Hangzhou Metro, based on long-term field monitoring. The settlement process is divided into three stages—immediate construction settlement, time-dependent additional settlement, and long-term consolidation—each associated with distinct levels of energy input, grouting demand, and embodied-carbon release. Peck’s Gaussian function is used to model transverse settlement troughs, and Gaussian superposition is applied to separate the contributions of the leading and trailing tunnels. The results indicate that the trailing shield induces ahead-of-face settlement at approximately two excavation diameters and produces a deeper–narrower settlement trough due to cumulative disturbance within the overlapping interaction zone. A ratio-type indicator, the Twin-Tunnel Interaction Ratio (TIR), is proposed to quantify disturbance intensity and reveal its environmental implications. High TIR values correspond to amplified ground response, prolonged stabilization, repeated compensation grouting, and increased embodied carbon during construction. Reducing effective TIR through coordinated optimization of shield attitude, face pressure, and grouting parameters can improve both deformation control and carbon efficiency. The proposed framework links geotechnical behaviour with environmental performance and provides a practical basis for risk-controlled, energy-efficient, and low-carbon management of sequential shield tunnelling. Full article

Low-melting bioabsorbable polymers, such as poly(caprolactone-co-lactide) (PCLA), hold significant promise for biomedical applications. However, achieving high-precision micro- and nanotopographical functionalization remains a formidable challenge due to the material’s susceptibility to thermal deformation during conventional thermal molding processes. In this study, functional microstructured PCLA coatings were engineered via low-temperature nanoimprint lithography utilizing a TiO₂–SiO₂ gas-permeable mold. These molds were synthesized via a sol–gel method utilizing titanium dioxide and silicon precursors. The gas-permeable nature of the mold facilitated the efficient evacuation of trapped air and volatiles during the imprinting process, enabling the high-fidelity replication of microstructures (1.3 μm height, 3 μm pitch) and nanostructured PCLA coatings featuring linewidths as narrow as 600 nm. The resultant microstructured PCLA coatings demonstrated modulated surface wettability, evidenced by an increase in water contact angles from 70.1° to 91.4°, and exhibited enhanced FD4 elution kinetics. These results confirm morphology-driven functionalities, specifically hydrophobicity and controlled release capabilities. Collectively, these findings underscore the efficacy of this microfabrication approach for polycaprolactone-based materials and highlight its potential to catalyze the development of high-value-added biomaterials for advanced medical and life science applications. This study establishes a foundational framework for the practical deployment of next-generation bioabsorbable materials and is anticipated to drive innovation in precision medical manufacturing. Full article

Pressed-in ventilation provides the possibility of implementing fire smoke control in tunnels during construction. In this study, the impact of the velocity at the air duct outlet, the heat release rate (HRR), and the tunnel geometry on the longitudinal temperature decay of the ceiling (ΔT) and smoke’s back-layering length (SBL) is investigated, using a reduced-scale experiment and the Fire Dynamics Simulator (FDS, version 6.7.6). The results indicate that an increase in the velocity at the air duct outlet and a decrease in the HRR lead to a reduction in the value of both ΔT and SBL in the main tunnel. Predictive models for the dimensionless longitudinal temperature decay of the ceiling and the dimensionless SBL are proposed. Near the fire source, the predicted SBL is relatively high due to thermal radiation. The research results provide valuable references for preventing tunnel fires during construction. Full article

Agro-industrial chestnut waste derived from chestnut processing is usually discharged without further use. However, these residues are attractive due to their high-value composition, rich in sugars and lignin. Among these residues, chestnut burrs (CB) represent a promising feedstock for biorefinery applications aimed at maximizing the valorization of their main constituents. In this study, we propose an environmentally friendly approach based on deep eutectic solvents (DES) formed by choline chloride and urea (ChCl/U) (1:2, mol/mol) for the selective deconstruction of lignocellulosic architecture, followed by enzymatic hydrolysis to release second-generation (2G) fermentable sugars. Pretreatments were applied to raw CB, washed CB (W-CB), and the obtained solid fraction after prehydrolysis (PreH). Structural and morphological modifications, as well as crystallinity induced by DES pretreatment, were characterized using attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR), field emission scanning electron microscopy (FE-SEM), and X-ray diffraction (XRD). Remarkable results in terms of effectiveness and environmental friendliness on saccharification yields were achieved for PreH subjected to DES treatment for 8 h, reaching approximately 60% glucan and 74% xylan conversion under the lower enzyme loading (23 FPU/g) and liquid-to-solid ratio (LSR) of 20:1 studied. This performance significantly reduces DES pretreatment time from 16 h to 8 h at mild conditions (100 °C), lowers the LSR for enzymatic hydrolysis from 30:1 to 20:1, and decreases enzyme loading from 63.5 FPU/g to 23 FPU/g, therefore improving process efficiency and sustainability. Full article

A new iodine–dextrin–lithium complex (IDLC) was synthesized and structurally characterized as a hybrid supramolecular system combining antiseptic, stabilizing, and biocompatible components. The compound integrates iodine as the primary antimicrobial agent, lithium as a coordination and stabilization element, and dextrin as a biodegradable polysaccharide matrix enabling sustained release. Physicochemical analyses confirmed the formation of a uniform, thermally stable complex. Biological evaluation revealed strong bactericidal activity, with minimum bactericidal concentrations (MBCs) ranging from 1.95 to 15.63 µg mL⁻¹ against both Gram-positive and Gram-negative pathogens, including multidrug-resistant Staphylococcus aureus and Acinetobacter baumannii. Cytotoxicity studies revealed moderate, concentration-dependent effects on human peripheral blood mononuclear cells (CC₅₀ = 0.23–0.48 mg/mL; 11.7–24.4 μg I/mL) and low toxicity toward MDCK cells (CC₅₀ = 10–20 mg/mL; 507–1014 μg I/mL), confirming a favorable safety profile. IDLC exhibited cytotoxic effects on tumor cell lines (HepG2, HeLa, AGS, K562, and H9) as well as on the normal MeT-5A cell line; however, the CC₅₀ values are similar, and selectivity indices are close to 1, indicating no selective cytotoxicity toward tumor cells. Thus, IDLC demonstrates non-specific cytotoxicity at high concentrations, consistent with its iodine content. The research confirms that iodine can be effectively stabilized within a dextrin-lithium framework to yield a biologically active, thermally resistant complex, suitable for pharmaceutical use. Full article

Peptides are short amino acid chains that can be released from proteins through hydrolysis or fermentation, exhibiting various biological activities, including antioxidant, antihypertensive, anti-inflammatory, and anticancer effects. Generally, these compounds are extracted from food products. However, to maximize resource utilization under the premise of sustainability in favor of a circular economy, there is an interesting approach to obtaining peptides from sub-utilized parts and non-food products of food production and processing, based on the grade at which they can be valued. These by-products may contain large quantities of protein that can be utilized. Although some may have a low protein content, they stand out for the quality and proportion of their amino acids, which provide properties with functional applications. This revision approaches some of the most recent reports on isolated peptides from residues, by-products, or underutilized parts from plant, animal, or sea origin; the conventional methods and alternative technologies to isolate them from the origin matrixes; the methods to purify and identify them, the biological activities they perform, as well as a brief description of the application fields of these compounds and the challenges that their application faces in biomedicine and the food industry. Full article

Platinum-based drugs play a pivotal role in contemporary cancer treatment, but their therapeutic utility is often limited by acquired resistance. The diiodido analog, cis-[PtI₂(NH₃)₂] is a promising derivative that has demonstrated the ability to overcome cisplatin resistance in vitro. To establish the molecular basis for this superior activity, we integrated experimental (NMR) spectroscopy with computational density functional theory (DFT) methods to precisely and comparatively understand the drug activation mechanisms. Comparative ¹⁴N NMR experiments elucidated the initial ligand substitution step, confirming halide displacement and a markedly higher tendency for ammonia release from cis-[PtI₂(NH₃)₂], particularly when reacting with sulfur-containing amino acids. Complementary DFT calculations determined the substitution energy values, revealing that the superior leaving-group ability of iodide results in a thermodynamically more favorable activation. Conceptual DFT parameters (softness, hardness, and Fukui indices) further demonstrated that initial substitution induces a strong trans effect, leading to the electronic sensitization of the remaining iodide ligand. This strong agreement between computational predictions and experimental data establishes a coherent molecular activation mechanism for cis-[PtI₂(NH₃)₂], demonstrating that iodide substitution promotes both thermodynamic and electronic activation of the platinum center, which is the key to its distinct pharmacological profile and ability to circumvent resistance. Full article

The search for multifunctional wound dressings that combine structural integrity with biological activity remains an important challenge in modern biomedicine. In this study, electrospun chitosan/polyethylene oxide (CS/PEO) nanofibers incorporating Centella asiatica extract were developed and evaluated in vitro as potential wound-healing materials. Nanofibers were fabricated using various CS/PEO ratios, and the 1:2 w/w composition loaded with 1% extract was selected as the optimal formulation based on morphological homogeneity and processing efficiency. Comprehensive characterization demonstrated that the nanofiber matrix provided sustained release of asiaticosides over several days, fitting best with Hixson–Crowell and Higuchi kinetic models, suggesting a combined diffusion–erosion mechanism. Biological assays confirmed that the optimized formulation displayed strong antioxidant and anti-inflammatory activity, with synergistic effects observed between chitosan and C. asiatica. Moreover, chitosan contributed intrinsic antimicrobial properties against Staphylococcus aureus and Klebsiella pneumoniae, while the extract provided additional antioxidant and regenerative potential. Biocompatibility studies in human fibroblasts showed no cytotoxic effects, and scratch assays confirmed that extract-loaded nanofibers significantly accelerated wound closure compared to the control and CS/PEO base. Taken together, the results highlight the potential of CS/PEO nanofibers with C. asiatica extract as multifunctional wound dressings that integrate structural support, controlled release, antimicrobial protection, and regenerative bioactivity. Future work should address in vivo evaluation, scale-up of electrospinning, and potential incorporation of synergistic antimicrobial agents to further enhance clinical applicability. This approach underlines the value of combining natural product pharmacology with biopolymer engineering in the design of next-generation wound-healing biomaterials. Full article

Introduction: The anonymisation of Personal Data (PD) and its release as Open Data (OD) hold considerable potential for innovation across health, research, public administration, and the economy. However, practical experiences regarding data anonymisation and OD publication remain underexplored in Germany. This study empirically investigates the current state of anonymised data practices, the barriers to implementation, and the desired support mechanisms for publishing formerly PD as OD. Methods: Embedded in a mixed-methods approach, this cross-sectional study examines research interest in the collection, processing, and use of anonymised data, as well as potential barriers and support services for the anonymisation and publication of former PD. A nationwide online survey was conducted in October–November 2024 via LimeSurvey. A total of 215 responses were included in the descriptive analysis. Results: The findings indicate limited experience with PD anonymisation and OD publication across industries. The potential added value of these processes was often not fully recognised, and data-handling responsibilities were rarely standardised. Data collectors, data protection officers, and IT departments were identified as the most frequently involved parties in these processes. Technical and educational support were the most desired forms of assistance. Discussion: To foster broader OD utilisation, stakeholders require comprehensive support. According to the sample, specific training and further education on the anonymisation and publishing process, as well as the desired software, are most important. Developing standardised process descriptions that integrate ethical and legal considerations, supported by national networks or governmental institutions, could significantly enhance the responsible and effective use of anonymised OD in Germany. Full article

The diesel substitution ratio is a key parameter influencing the combustion characteristics and energy conversion efficiency of hydrogen diesel dual-fuel free-piston engines. This study develops a thermodynamic hydrodynamic coupled model for a dual-fuel free engine to investigate the effects of five substitution ratios (15%, 20%, 25%, 30%, and 35%) on in-cylinder mixture formation, combustion characteristics, and emission performance. The key novelty of this work lies in employing this fully coupled combustion-dynamics model to systematically optimize the hydrogen–diesel substitution ratio, which explicitly captures the critical feedback between combustion and the piston’s unique motion. The cumulative heat release served as the key quantitative metric. The analyzed parameters included the gas mixture fraction, turbulent kinetic energy, flow trajectories, in-cylinder pressure and temperature, combustion reaction rate, unburned equivalent ratio, cumulative heat release and its rate, heat release rate, and emission mass. The results demonstrate that the engine’s overall performance is optimal at a substitution ratio of 25%. At this ratio, a peak volumetric mixture fraction of 0.0088 was achieved with a broad distribution range, indicating significantly improved spatial fuel uniformity. The flow field exhibited organized swirl patterns that enhanced fuel dispersion. The peak in-cylinder pressure reached 7.2 MPa, which was 0.044 MPa higher than that of the 20% group. The combustion temperature remained stable, with a peak value of 1606 K, exceeding the 20% and 30% groups by 7 K and 16 K, respectively. The heat release phase was well-synchronized with the piston motion, ensuring a high proportion of premixed combustion for thorough fuel oxidation. Although nitrogen oxide (NOx) emissions were slightly higher, the reduction in soot was substantially greater than in the 20% group, leading to overall superior performance compared to the other substitution ratios. This study develops a thermodynamic hydrodynamic coupled model for a dual-fuel free-piston engine by leveraging the interaction between piston motion and combustion. This paper presents a novel strategy for optimizing the substitution ratio in a free piston engine via a fully coupled combustion-dynamics model. Full article

The oral cavity acts as the anatomical gateway to the respiratory tract, sharing both microbiological and pathophysiological links with the lower airways. Although radiotherapy is a cornerstone treatment for lung cancer, reliable oral microbiome biomarkers for predicting patient outcomes remain lacking. We analyzed the oral microbiome of 136 lung cancer patients and 199 healthy controls across discovery and two validation cohorts via 16S rRNA sequencing. Healthy controls exhibited a significantly higher abundance of Streptococcus compared to patients (p = 0.049, p < 0.001, p < 0.001, respectively). The structure of the microbial community exhibited substantial dynamic changes during treatment. Responders showed enrichment of Rothia aeria (p = 0.027) and Prevotella salivae (p = 0.043), associated with prolonged overall survival (OS) and progression-free survival (PFS), whereas non-responders exhibited elevated Porphyromonas endodontalis (p = 0.037) correlating with shorter OS and PFS. According to Analysis of Compositions of Microbiomes with Bias Correction 2 (ANCOM-BC2) analysis, Akkermansia and Alistipes were nearly absent in non-responders, while Desulfovibrio and Moraxella were virtually absent in responders. A diagnostic model based on Streptococcus achieved area under the curve (AUC) values of 0.85 (95% CI: 0.78–0.91) and 0.99 (95% CI: 0.98–1) in the validation cohorts, and a response prediction model incorporating Prevotella salivae and Neisseria oralis yielded an AUC of 0.74 (95% CI: 0.58–0.90). Furthermore, in small cell lung cancer, microbiota richness and diversity were inversely correlated with Eastern Cooperative Oncology Group (ECOG) performance status (p = 0.008, p < 0.001, respectively) and pro-gastrin-releasing peptide (ProGRP) levels (p = 0.065, p = 0.084, respectively). These results demonstrate that lung cancer-associated oral microbiota signatures dynamically reflect therapeutic response and survival outcomes, supporting their potential role as non-invasive biomarkers for diagnosis and prognosis. Full article

Mt. Etna is the highest and most active stratovolcano in Europe, located in Catania (Sicily, Italy). Its persistent degassing, frequent explosions, and lava flows release large amounts of ash and gases into the atmosphere. This study aimed to assess whether chronic exposure to local volcanic emissions leads to an increased internal dose of trace elements (As, Ba, Be, Bi, Cd, Co, Cr, Cu, Hg, Li, Mn, Mo, Ni, Pb, Sb, Se, Sn, Sr, Tl, U, V, W, Zn) in Catania adult residents. To this end, urine samples were collected from 167 individuals residing in Catania and compared with 193 residents of other Sicilian areas located farther from the volcano. Results revealed significantly higher urinary concentrations of As, Hg, Mn, Pb, and Tl in the exposed group, suggesting volcanic activity as a relevant source of exposure. The levels of the other elements were instead affected by other factors such as lifestyle habits and the consumption of specific foods and beverages. The urinary concentrations of trace elements were consistent with reference values reported in other European studies, and the levels remained well within the health-based guidance values. There is evidence of an increased internal dose of a few elements in the Sicilian population exposed to volcano activity, but the observed increases are unlikely to pose a significant health risk. Full article

Rising residential land prices push up housing prices and worsen credit misallocation. These patterns emerge amid cyclical real estate fluctuations and heavy land-based public finance. Such pressures undermine macroeconomic stability and sustainable land-use. The land price circuit breaker is widely applied with a price cap and state dependence, yet its trigger mechanism and interaction with inflation targeting remain underexplored. This study addresses three core questions. First, how does the circuit breaker’s discrete trigger and rule-switching logic differ from traditional static price ceilings? Second, can the mechanism, via the collateral channel, restrain excessive land price hikes, improve credit allocation, and, thereby, stabilize land price dynamics and long-run macroeconomic performance? Third, how does the circuit breaker interact with inflation targeting, and through which endogenous channels does a strict target dampen housing prices and raise activation probability? This study develops a multi-sector DSGE model with an embedded land price circuit breaker. The price cap is modeled as an occasionally binding constraint. A dynamic price band and trigger indicator capture the policy’s switch between slack and binding states. The framework incorporates interactions among local governments, the central bank, developers, and households. It also links firms and the secondary housing market. Under different inflation-targeting rules, this study uses impulse responses, an event study, and welfare analysis to assess trigger conditions and macroeconomic effects. The findings are threefold. First, a strict inflation target increases the probability of a circuit breaker being triggered. It channels housing-demand shocks toward land prices and creates a “nominal anchor–relative price constraint” linkage. Second, once activated, the circuit breaker narrows the gap between land price and house-price growth. It weakens the procyclicality of collateral values. It also restrains credit expansion by impatient households. These effects redirect credit toward firms, improve corporate financing, reduce the decline in investment, and accelerate output recovery. Third, the circuit breaker limits new land supply and shifts demand toward the secondary housing market. This generates a supply-side effect that releases existing stock and stabilizes prices, thereby weakening the amplification mechanism of housing cycles. This study identifies the endogenous trigger logic and cross-market transmission of the land price circuit breaker under a strict inflation target. It shows that the mechanism is not merely a price-management tool in the land market but a systemic policy variable that links the real estate, finance, and fiscal sectors. By dampening real estate procyclicality, improving credit allocation, and stabilizing macroeconomic fluctuations, the mechanism offers new insights for sustainable land-use policy and macroeconomic stabilization. Full article

Background/Objectives: To analyze the behavior and release of bisphenol A (BPA) in the saliva of patients wearing clear aligners, and to evaluate differences in BPA levels between patients treated with aligners from the market-leading brand and those treated with in-office aligners. For the in-office group, 0.762 mm (0.30”) thick thermoforming sheets from Ortolan^® were used to fabricate the aligners. Methods: Patients about to begin orthodontic treatment with clear aligners in the Master’s degree program in Orthodontics at the University of Valencia were recruited for this prospective observational study. Patients were divided into two groups based on the type of aligners: The Invisalign^® group and the In-Office aligner group. Four saliva samples were taken from each patient at different times during treatment, with a one-week follow-up. The samples were stored at −80 °C and analyzed using mass spectrometry. Results: A total of 24 patients were included in the study, with 12 patients in each group. A statistically significant difference was found between baseline BPA levels and the increase observed half an hour after bonding the attachments. After a week, values returned to pre-treatment levels. Furthermore, BPA levels changed significantly during the follow-up period and were similar in both groups. Conclusions: An immediate increase was observed when the attachments were bonded with both treatments; however, differentiation from the ‘peak’ and recovery to baseline values was faster in patients treated with In-Office aligners. In those treated with Invisalign^®, after the placement of the aligners, values recorded were not significantly different from baseline, nor from the previous peak. Full article

Background/Objectives: Wound healing requires simultaneous pain control, inflammation management, infection prevention, and tissue regeneration. This study aimed to develop and evaluate in vitro a non-contact thermosensitive spray hydrogel combining lidocaine for rapid analgesia and allantoin for tissue repair. Methods: The effects of chitosan and Poloxamer 407 on viscosity, spray diameter, and bioadhesion ability of hydrogels were optimized using response surface methodology. Lead formulations (S1 and S2) were selected via a desirability function within the software. The pH, gelation temperature (T_G), rheological behavior, sprayability, bioadhesion, and lidocaine release using the dialysis bag method were assessed. The in vitro cytotoxicity, anti-inflammatory activity (TNF-α), and cell migration (scratch assay) of the formulations were investigated. Results: The viscosity values (42.7–58.7 mPa·s) indicated suitability for spraying at room temperature. T_G was 28.7 ± 0.6 °C (S1) and 29.3 ± 0.3 °C (S2), enabling rapid sol–gel transition at skin temperature. The lidocaine release reached 95–100% within 120 min. S2 exhibited lower viscosity and wider spray diameter, improving applicability on larger wound areas. In vitro cytotoxicity, scratch assay, and inflammatory marker analyses demonstrated that the optimized sprayable hydrogels exhibited a biocompatible and cell-healing profile. Conclusions: The developed thermosensitive spray hydrogel enables the combined delivery of lidocaine and allantoin, rapid gelation at body temperature, and touch-free administration. Its suitable viscosity and sprayability, and fast lidocaine release profile indicate high patient compliance and a significant advantage over conventional cream/ointment formulations, particularly regarding painless application, reduced contamination risk, enhanced therapeutic potential, and confirmed in vitro biocompatibility with supportive effects on keratinocyte behavior. Full article