Search Results (15,566)

Enterocytozoon bieneusi is the most frequently diagnosed microsporidian parasite in humans and a recognized cause of diarrheal disease, particularly in immunocompromised individuals. Its broad host range, which includes livestock, companion animals, and wildlife, highlights its zoonotic potential and warrants careful epidemiological assessment. This narrative review synthesizes available data on the occurrence and genetic diversity of E. bieneusi in European domestic ungulates (cattle, pigs, sheep, goats, horses, and water buffaloes) and pets (dogs and cats), aiming to provide an integrated perspective on animal reservoirs and their relevance for public health. Publications retrieved from the Web of Science Core Collection database were systematically screened, and country-specific results were extracted, emphasizing prevalence rates, genotype distributions, and zoonotic implications. Across Europe, cattle and pigs emerged as the most studied hosts, frequently harboring zoonotic group 1 genotypes such as I, J, BEB4, BEB6, and EbpA, while small ruminants, horses, and buffaloes remain comparatively undocumented. In pets, the dog-adapted genotype PtEb IX was predominant, but several zoonotic genotypes were also identified. Overall, the current evidence confirms the wide host range of E. bieneusi in Europe but also reveals significant data gaps compared to regions such as China, underlining the need for broader surveillance and harmonized molecular approaches within a One Health framework. Full article

Mastitis caused by Escherichia coli and Streptococcus uberis remains one of the leading causes of antimicrobial use in dairy cattle, contributing to resistance development and economic losses. This study evaluated the therapeutic potential of the natural flavonoid morin in clinical mastitis in dairy cows. The in vitro antimicrobial activity of morin (1–3% w/v) was assessed by disk diffusion, and the 3% formulation was selected for an in vivo field trial. Seventy-two Holstein–Friesian cows with mastitis caused by E. coli or S. uberis were randomly assigned to one of three intramammary treatments: 3% morin, phosphate-buffered saline, or an antibiotic, serving as a positive control. Somatic cell count (SCC) and clinical scores were monitored for seven days. In E. coli infections, morin significantly reduced somatic cell scores at 144 h and 168 h and improved clinical scores from 48 h onward, showing efficacy comparable to antibiotics. In S. uberis mastitis, morin induced clinical improvement at 96–168 h but resulted in slower and smaller SCC reduction than antibiotic control therapy. Phosphate-buffered saline produced no significant changes. These results indicate that morin exerts anti-inflammatory and supportive effects in bovine mastitis, particularly in Gram-negative infections, but is less effective against S. uberis. Further studies on pharmacokinetics, bacteriological cure rates, and optimized formulations are warranted to confirm its clinical utility. Full article

Floods, especially in urbanised areas, incur enormous economic and social losses. The structural flood management is often limited by urbanization and environmental issues. Following the catastrophic flood events of 1997 and 2010, a relatively large dry polder was constructed in Racibórz Dolny, Poland, with the highest flood retention capacity in Central Europe. During the 2024 flood in Czechia and Poland, the polder was filled to 80%, which significantly reduced the floodwave crest on the Odra River (by 1.65 m), halved the peak discharge, and delayed the floodwave passage by two days according to hydrological calculations. The operation of the polder enables multifunctional use of the river valley—ranging from agriculture and mineral extraction to environmental protection—without the need for permanent water impoundment. Aggregate extraction carried out within the basin contributed to shaping the reservoir, reducing the demand for transport and construction materials, while the overburden was reused for engineering and reclamation purposes. Mining activities between 2007 and 2023 increased the retention capacity of the polder by 13%, providing an example of rational environmental resource management combined with effective flood protection. The findings demonstrate that integrating retention functions with mineral resource management represents an efficient and sustainable approach to mitigating flood impacts in large European river valleys. Full article

Essential oils (EOs) have gained recognition as promising alternatives to antibiotics due to their positive effects on bird growth performance, enhanced meat quality, and improved overall health, without producing the negative consequences associated with antibiotics. This study evaluated the effects of dietary supplementation of tea tree (TTEO) and thyme (TEO) EOs, individually or in combination with the probiotic BioPlus 2b (Bacillus subtilis and Bacillus licheniformis), on poultry broiler performance, including the meat quality. A total of 240 ROSS 308 broilers were assigned to eight dietary treatments over a 35-day trial. Parameters such as body weight (BW), feed conversion ratio (FCR), carcass portion, drip loss, and meat pH were evaluated. TTEO had a significant (p ≤ 0.05) impact on final carcass and breast portion, while in combination with probiotics, specifically TTEO with BioPlus significantly (p ≤ 0.05) reduced meat drip loss. GC-MS analysis identified terpinen-4-ol and γ-terpinene as the major constituents of TTEO, and thymol and carvacrol as the major constituents of TEO. In conclusion, combinations of TTEO, TEO, and probiotics can have beneficial effects on chicken raw meat quality, providing a complementary benefit to the industry and representing a viable alternative to conventional agents. Full article

This study evaluated the structural, convergent, and discriminant validity, invariance, and internal consistency of the Generalized Anxiety Disorder—7 (GAD-7), Patient Health Questionnaire—9 (PHQ-9), Somatic Symptom Scale—8 (SSS-8), and Satisfaction with Life Scale (SWLS) among 910 students at the National Autonomous University of Honduras. Mental health issues are common among university students, with anxiety, depression, and somatization often co-occurring from a transdiagnostic perspective. Life satisfaction, meanwhile, is recognized as a protective factor for mental well-being. In response to rising psychological distress among university students, reliable mental health screening tools are critical for early detection and intervention. Confirmatory factor analysis (CFA) supported the unidimensional structure of each scale, while multigroup CFA demonstrated gender invariance. Women reported higher anxiety, depression, and somatization scores, whereas men had higher life satisfaction. Internal consistency, measured by McDonald’s Omega (Ω) and composite reliability, was excellent for GAD-7 (Ω = 0.927), PHQ-9 (Ω = 0.919), and SSS-8 (Ω = 0.873). Convergent and discriminant validity were supported through significant correlations: GAD-7, PHQ-9, and SSS-8 were positively correlated with each other, and negatively correlated with SWLS. These findings confirm that the four scales are psychometrically sound instruments for evaluating mental health in Honduran university students. Full article

Clear aligners have gained popularity in orthodontics due to their aesthetics, comfort, and removability; however, their prolonged intraoral wear and frequent removal–reinsertion cycles create favorable conditions for microbial colonization. This in vitro study evaluated the efficacy of seven commercially available mouthwash formulations in inhibiting biofilms of Streptococcus mutans, Streptococcus oralis, and Candida albicans formed on four different clear aligner materials. Standardized aligner fragments were incubated for 24 h with microbial suspensions to allow biofilm formation, treated for 1 min with one of the mouthwashes, and then assessed for residual viability through spectrophotometric optical density measurements after a further 24 h incubation. Biofilm inhibition varied according to both mouthwash composition and aligner material. The chlorhexidine-based rinse (MW-D) consistently showed the highest inhibition across microorganisms, while the fluoride–cetylpyridinium chloride rinse (MW-B) performed strongly for S. oralis and C. albicans. An essential oil-based formulation with xylitol (MW-G) showed notable antifungal activity against C. albicans. Monolayer polyurethane aligners generally achieved higher inhibition rates than multilayer or copolyester-based materials. These findings indicate that antimicrobial efficacy on aligners depends on both mouthwash type and material, supporting a tailored approach to biofilm management in clear aligner therapy to reduce the risk of caries, periodontal disease, and candidiasis. Full article

Industrial hemp (Cannabis sativa L.; Cannabaceae), traditionally cultivated for fiber, also represents a valuable source of nutrient-rich seed oil. In this study, cold-pressed hemp seed oils from three Romanian varieties (Teodora, Silvana, and Armanca) were evaluated for their fatty acid composition, minor bioactive constituents, antioxidant activity, growth-promoting property toward probiotic strains in vitro, and molecular docking interactions with probiotic targets. Gas chromatography revealed a fatty acid profile dominated by linoleic (49.4–51.9%), oleic (16.3–22.8%), and α-linolenic acids (9.8–14.4%), resulting in favorable PUFA/SFA ratios (5.17–6.39) and ω-6/ω-3 ratios (3.93–5.53). The oils also contained phenolics (118–160 mg GAE/kg), chlorophylls (6.18–8.31 mg/kg), and carotenoids (2.58–3.37 mg/kg), which contributed to their antioxidant activity (DPPH inhibition 35.92 µM TE/100 g–43.37 µM TE/100 g). Broth microdilution assays against Lacticaseibacillus rhamnosus GG, L. paracasei ATCC BAA-52, and L. acidophilus ATCC 4356 demonstrated strain- and dose-dependent potential to promote probiotic growth under in vitro conditions. While L. rhamnosus and L. paracasei were inhibited at low concentrations and only mildly stimulated at higher levels, L. acidophilus showed robust growth promotion, reaching +54.7% effect and CP = 1.55 with Teodora oil at 16 mg/mL. Molecular docking highlighted strong binding affinities of γ-linolenic and linoleic acids with key metabolic enzymes involved in probiotic metabolism (hydratase, enolase, glyceraldehyde-3-phosphate dehydrogenase, ribonucleoside hydrolase), forming stable hydrophilic and hydrophobic interactions which are explored in defining the stability of the ligand-protein complexes. These results indicate that both major fatty acids and minor bioactive constituents contribute to the nutritional and antioxidant value of Romanian hemp seed oils and reveal a potential to promote probiotic growth under in vitro conditions, as supported by complementary in silico evidence. Full article

The utilization of post-production and post-processing by-products aligns with current trends in sustainable fruit industry practices. Recovering valuable nutrients from such materials holds significant potential for the food, nutraceutical, pharmaceutical, and cosmetic sectors. Among these, cornelian cherry (Cornus mas L.) seeds represent a promising source of functional ingredients, particularly due to their oil’s rich nutritional and phytochemical profile. The seeds, accounting for approximately 9–10% of the fruit mass, yield an oil characterized by high levels of polyunsaturated fatty acids—mainly linoleic acid (≈67.5%) and oleic acid (≈20%)—alongside palmitic (≈5.8%) and stearic acids (≈2.1%). Linolenic acid content, however, shows notable variability (1.4–14.7%), influencing the oil’s omega-6/omega-3 ratio, which generally remains below 5:1. Cornelian cherry seed oil stands out among other stone fruit oils (e.g., rosehip, apricot, peach, cherry, plum) for its favorable fatty acid composition and absence of cyanogenic glycosides, making it safe for human consumption. Beyond its nutritional value, this oil exhibits biological activity and health-promoting potential, suggesting wide applicability in functional foods and nutraceutical formulations. Despite progress in characterizing seed composition—including proteins, lipids, carbohydrates, minerals, and tannins—knowledge gaps persist regarding the transfer of these compounds into the oil, particularly under cold-pressing conditions. Future studies should focus on optimizing extraction processes, assessing thermal treatment effects, and clarifying the variability of linolenic acid. Such research will support the sustainable exploitation of cornelian cherry by-products and the industrial-scale development of this high-value oil. Full article

Post-translational modifications (phosphorylation and glutathionylation) not only assure protein diversity but are also responsible for the controlling of the biological activity of selected proteins in health and disease. The aim of the study was to monitor the profile of changes in molecular weight of proteins undergoing selected post-translational modifications by measurement of the intensity of phosphorylation and glutathionylation within the pregnancy course and parturition in cows with and without the retention of foetal membranes. The intensity of selected post-translational modifications was measured in bovine placental tissues collected during pregnancy (2nd, 4th, 5th, and 7th month, n = 4 per month) and parturition (not-retained foetal membranes (NRFM, n = 6) and retained foetal membranes (RFM, n = 6). Placental tissues were homogenised and used for the Phosphoprotein Phosphate Estimation Assay Kit and Western blotting analyses with adequate antibodies. The content of phosphorylated proteins was significantly higher (p < 0.05) in the 2nd month as compared to other months, both in the maternal and foetal parts of the placenta. Moreover, no significant differences were observed between NRFM and RFM samples. The results of Western blotting showed the shift in molecular weight and indirect content of phosphorylated selected amino acids. Further research on the role of post-translational modifications in pregnancy and parturition may give new insight into their biochemical regulation. Full article

Medicinal mushrooms are recognised as a source of bioactive polysaccharides and proteins with potential health benefits. This study presents the first comparative evaluation of wheat bread fortified with powdered fruiting bodies of three medicinal mushroom species: Hericium erinaceus (Lion’s Mane), Ganoderma lucidum (Reishi), and Ophiocordyceps sinensis (Cordyceps). Wheat flour was partially substituted at levels of 3–12%, and the resulting breads were analysed for (1→3)(1→6)-β-D-glucan content as well as in vitro protein and starch digestibility. Mushroom enrichment significantly increased β-glucan concentration in bread, with the greatest enhancement observed for 12% Reishi substitution (5.67% d.m.). Starch digestibility decreased across all fortified breads, accompanied by a substantial increase in resistant starch, particularly for Reishi bread (+427% relative to control). Protein digestibility was also reduced, most prominently in Cordyceps bread (−32.7 percentage points), although these products still provided up to ~52% more total protein than the control. The results confirm that incorporating medicinal mushrooms into wheat bread effectively increases its content of bioactive components, such as β-glucans, resistant starch and protein, indicating its potential as a nutrient-enriched bakery product with improved functional value. Full article

The crystalline structure of cellulose plays a crucial role in its reactivity, which is particularly important in biomass processing involving enzymatic hydrolysis. This study investigates the effect of low-frequency ultrasound (40 kHz) on the efficiency of enzymatic hydrolysis of cellulose in poplar wood and its structure, with a focus on its crystallinity and susceptibility to enzymatic hydrolysis. Two experimental pathways were explored: ultrasound-assisted hydrolysis of raw wood and isolated cellulose. Structural modifications were assessed using Fourier-transform infrared spectroscopy to determine the Lateral Order Index (LOI) and Total Crystallinity Index (TCI), providing insight into the reorganisation of cellulose microstructure. To evaluate hydrolysis efficiency, glucose yields were quantified by high-performance liquid chromatography. The application of ultrasound to raw wood resulted in minimal improvement in sugar release, whereas pretreatment of extracted cellulose led to a modest acceleration of enzymatic hydrolysis. The results show that ultrasound-assisted hydrolysis of raw wood did not significantly increase glucose yield, reaching only 9.9 ± 0.3% and 10.1 ± 0.8% for two poplar hybrids. Only slight increases in TCI and no significant changes in LOI were observed after 4 h of ultrasonic exposure. It indicates limited disruption of the crystalline structure under the tested conditions. Full article

The effect of the high-pressure homogenization (HPH) process (one-stage; applied pressure of 10 MPa and 20 MPa) and furcellaran addition (0.25% and 0.50%, w/w) on the physicochemical and rheological properties of chocolate milk drinks was evaluated. Non-homogenized samples and the milk sample used to produce all model chocolate milk drink samples were also evaluated for comparison. The addition of furcellaran and the HPH process significantly influenced the monitored characteristics of the model samples. In particular, the addition of furcellaran caused an increase in shear stress and viscosity, and HPH also had a noticeable effect on these rheological properties. From the results obtained, it can be concluded that the model samples of chocolate milk drinks exhibited a pseudoplastic behavior. Furthermore, the results of the physicochemical analyses showed a slight increase in dry matter and total soluble solids contents due to furcellaran addition. The solid particle sedimentation values of the tested samples decreased due to furcellaran addition (up to 11.99%.). In addition to the effect on rheological properties, the HPH regime slightly increased the sample’s pH values (6.80–6.81). The application of HPH and furcellaran addition may appear advantageous in manufacturing chocolate milk drinks (or dairy-based suspensions) with enhanced physical, flow and sensory properties. Full article

In order to address the social, economic, and environmental challenges arising from urban development, some urban revitalization plans have been proposed. With the implementation of these plans, the spatial pattern of the region has also undergone corresponding changes. Some of the revitalization projects have driven economic growth while accompanied by ecological degradation, while others have achieved coordinated development and protection. This study selected eight urban revitalization cases, based on remote sensing (RS) and geographic information system (GIS), and used the Random Forest (RF) machine learning method to dynamically monitor the spatial changes in the region before and after revitalization through Land Use and Land Cover (LULC) analysis. The research results show that among the eight cases, only the revitalization cases located in Beijing and Swarzędz reflected an increase in water and vegetation areas, while the built-up area decreased. The other six cases located in Nanjing, Kraków, Wągrowiec, Swarzędz, Parczew, and Mosina all reflect the result of built-up areas encroaching water and vegetation areas. Full article

In recent years, microwave and ultrasound technology has been under extensive development in drying technologies. Researchers are constantly searching for improved solutions or alternatives to hot air drying. The goal of this work was to determine the intermittent action of ultrasound and microwaves on convective drying. An examination of five specific cases of stationary and nonstationary drying processes was conducted. The evolution of moisture content and drying rate over process time was discussed, and the average drying rate and time, drying constant, effective diffusion coefficient, and specific energy consumption were also compared. To identify the differences between the dried products, the quality characteristics such as: water activity, color, shrinkage, rehydration, polyphenol content, odor, and flavor of apples were analyzed. The results indicate that intermittent drying provides a good alternative to convective drying, including when combined with microwave and ultrasound treatments. Applying microwaves or ultrasound intermittently resulted in an increase in the effective diffusion coefficient (by 68%) and drying rate (by 117%) and a reduction in drying time (by 53%), compared to convective drying. This processing method resulted in lower energy consumption by up to 13% and well-preserved quality attributes—this could be very promising for the production of healthy, ready-to-eat apple snacks. Full article

Recent research efforts have focused on improving the performance of metal-oxide semiconductor (MOS) gas sensors through their thermal modulation using integrated heaters. This approach allows us to enhance the selectivity of measurements; however, the main challenge with this amelioration lies in interpreting the sensor response, which takes the form of complex patterns that require the application of advanced signal processing techniques. This study introduces a methodology for the quantitative determination of volatile compounds (ethanol and methanol at various concentrations ranging from 31 to 2000 ppm for each of these compounds) in mixtures using a single thermally modulated MOS gas sensor. The recorded responses of the detector were interpreted by combining two signal processing techniques: principal component analysis (PCA) for feature extraction, and artificial neural networks (ANNs) for predicting the levels of the tested volatile components. The proposed methodology demonstrated satisfactory performance achieving R² values at the level of 0.999 across all datasets (learning, test, validation) and low error metrics (RMSE = 11.6–14.4 ppm), thereby confirming the robustness and accuracy of the approach and its applicability in a wide range of fields where rapid, cost-effective, and precise detection of ethanol and methanol is essential. Full article