Search Results (341)

Obligate symbionts often exhibit some degree of co-speciation with their hosts. One prominent example is the symbiosis between termites and their wood-feeding hindgut protists. This symbiosis is mutually obligate, vertically inherited by anal feeding, and it predates the emergence of termites from their cockroach ancestors. Termites and their symbiotic protists might therefore be expected to have congruent phylogenies, but symbiont loss, transfer, and independent diversification can impact the coevolutionary history to varying degrees. Here, we have characterized the symbiotic protist communities of eight Neoisoptera species from three families in order to gauge the phylogenetic congruence between each lineage of protists and their hosts. Using microscopy and 18S rRNA gene sequencing of individually isolated protist cells, we identified protists belonging to the Parabasalia genera Pseudotrichonympha, Holomastigotoides, Cononympha, and Cthulhu. Pseudotrichonympha were present in all of the investigated termites, with a strong pattern of codiversification with hosts, consistent with previous studies. The phylogeny of Holomastigotoides indicates several instances of diversification that occurred independently of the hosts’ diversification, along with lineage-specific symbiont loss. Cononympha occurs only in Heterotermitidae and Psammotermes. Surprisingly, the small flagellate Cthulhu is widespread and exhibits cophylogeny with its hosts. This study demonstrates that different symbiont lineages can show different coevolutionary patterns, even within the same host. Full article

This study presents a cost-effective, carbon-negative construction material for affordable housing, developed entirely from locally available agricultural wastes: rice husk ash, wood ash, and rice straw—materials often problematic to dispose of in many African regions. Rice husk ash provides high amorphous silica, acting as a strong pozzolanic agent. Wood ash contributes calcium oxide and alkalis to serve as a reactive binder, while rice straw functions as a lightweight organic filler, enhancing thermal insulation and indoor climate comfort. These materials undergo natural pozzolanic reactions with water, eliminating the need for Portland cement—a major global source of anthropogenic CO₂ emissions (~900 kg CO₂/ton cement). This process is inherently carbon-negative, not only avoiding emissions from cement production but also capturing atmospheric CO₂ during lime carbonation in the hardening phase. Field trials in Kenya confirmed the composite’s sufficient structural strength for low-cost housing, with added benefits including termite resistance and suitability for unskilled laborers. In a collaboration between the University of Tartu and Kenyatta University, a semi-automatic mixing and casting system was developed, enabling fast, low-labor construction of full-scale houses. This innovation aligns with Kenya’s Big Four development agenda and supports sustainable rural development, post-disaster reconstruction, and climate mitigation through scalable, eco-friendly building solutions. Full article

Mycelium-based composites (MBCs) are an emerging category of cost-effective and environmentally sustainable materials that are attracting significant research and commercial interest across various industries, including construction, manufacturing, agriculture, and biomedicine. These materials harness the natural growth of fungi as a low-energy bio-fabrication method, converting abundant agricultural by-products and waste into sustainable alternatives to energy-intensive synthetic construction materials. Their affordability and eco-friendly characteristics make them attractive for both research and commercialisation. Currently, mycelium-based foams and sandwich composites are being actively developed for applications in construction. These materials offer exceptional thermal insulation, excellent acoustic absorption, and superior fire safety compared to conventional building materials like synthetic foams and engineered wood. As a result, MBCs show great potential for applications in thermal and acoustic insulation. However, their foam-like mechanical properties, high water absorption, and limited documentation of material properties restrict their use to non- or semi-structural roles, such as insulation, panelling, and furniture. This paper presents a comprehensive review of the fabrication process and the factors affecting the production and performance properties of MBCs. It addresses key elements such as fungal species selection, substrate choice, optimal growth conditions, dehydration methods, post-processing techniques, mechanical and physical properties, termite resistance, cost comparison, and life cycle assessment. Full article

This research aimed to evaluate the biological resistance to xylophagous organisms and the dimensional stability related to water absorption in plastic wood panels manufactured by compression molding and produced with pine wood and recycled thermoplastics. The wood–plastic composites (WPCs) were prepared from 50% pine sawdust and 50% recycled plastics (polyethylene terephthalate-PET, high-density polyethylene-HDPE, and polypropylene-PP). The thickness swelling test was carried out by immersing of the WPC samples in water at room temperature (25–30 °C) and evaluating the total change in WPC thickness after 1500 h (≈9 weeks or two months). In addition, the coefficient of initial swelling was evaluated to verify the variability of the swelling. For the biological resistance evaluation of the WPCs, tests were carried out with soil or arboreal termites (Nasutitermes corniger) and drywood termites (Cryptotermes brevis). The WPC loss of mass and termite mortality were evaluated. The use of PP promoted the best response to thickness swelling. The simple mathematical model adopted offers real predictions to evaluate the thickness of the swelling of the compounds in a given time. For some variables there were no statistical differences. It was shown that treatment 3 (T3) presented visual damage values between 0.4 for drywood termites and 9.4 for soil termites, in addition to 26% termite mortality, represented by the lowest survival time of 12 days. The developed treatments have resistance to termite attacks; these properties can be an important starting point for its use on a larger scale by the panel industries. Full article

Logs supplied in Papua New Guinea and Indonesia are predominantly sourced from fast-growing tree species of plantation forests. The timber primarily consists of sapwood, which is highly susceptible to biodeterioration. At a training center, CCA (chromated copper arsenate) is still used for wood preservation, while in the wood industry, ACQ (alkaline copper quaternary) is commonly applied to enhance the service life of timber. In the future, polystyrene impregnation or other non-biocidal treatments could potentially serve this purpose. This study aimed to determine the discoloration and resistance of polystyrene-impregnated and CCA-preserved woods. Wood samples, Anisoptera thurifera and Octomeles sumatrana from Papua New Guinea, and Anthocephalus cadamba and Falcataria moluccana from Indonesia, were used. The wood samples were treated with polystyrene impregnation, CCA preservation, or left untreated, then exposed at the PNG Forest Research Institute site for four months. After treatment, the color change in polystyrene-impregnated wood was minor, whereas CCA-preserved wood exhibited a noticeably different color compared to untreated wood. The average polymer loading for polystyrene-impregnated wood reached 147%, while the average CCA retention was 8.4 kg/m³. Densities of untreated-, polystyrene-, and CCA-wood were 0.42, 0.64, and 0.45 g/cm³, respectively, and moisture contents were 15.8%, 9.4%, and 13.4%, respectively. CCA preservation proved highly effective in preventing termite attacks; however, CCA is hazardous to living organisms, including humans. Polystyrene impregnation also significantly improved wood resistance to subterranean termites, as indicated by lower weight loss and a higher protection level compared to untreated wood. Additionally, polystyrene treatment is nonhazardous and safe for living organisms, making it a promising option for enhancing wood resistance to termite attacks in the future as an alternative to the biocides currently in use. Full article

Termites pose significant threats to the structural integrity of embankments due to their nesting and tunneling behavior, which leads to internal voids, water leakage, or even dam failure. This review systematically classifies and evaluates current termite detection techniques in the context of embankment maintenance, focusing on physical sensing technologies and biological characteristic-based methods. Physical sensing methods enable non-invasive localization of subsurface anomalies, including ground-penetrating radar, acoustic detection, and electrical resistivity imaging. Biological characteristic-based methods, such as electronic noses, sniffer dogs, visual inspection, intelligent monitoring, and UAV-based image analysis, are capable of detecting volatile compounds and surface activity signs associated with termites. The review summarizes key principles, application scenarios, advantages, and limitations of each technique. It also highlights integrated multi-sensor frameworks and artificial intelligence algorithms as emerging solutions to enhance detection accuracy, adaptability, and automation. The findings suggest that future termite detection in embankments will rely on interdisciplinary integration and intelligent monitoring systems to support early warning, rapid response, and long-term structural resilience. This work provides a scientific foundation and practical reference for advancing termite management and embankment safety strategies. Full article

A detailed morphological characterization is presented for Oriboglyphus maorianus gen. et sp. nov., a newly discovered modern representative of the relict family Schizoglyphidae, found in a nest of the termite Stolotermes ruficeps in New Zealand. This is the second extant schizoglyphid species known from modern material, and its discovery provides rare insight into a transitional stage in the evolution of astigmatid life cycles. The phoretic stage appears to be a tritonymph—rather than the typical deutonymph—based on key morphological traits including three-segmented palps, three pairs of genital papillae, and the presence of a pharynx. These features suggest that early astigmatid mites evolved multiple ontogenetic routes to dispersal, including tritonymphal, deutonymphal, and possibly adult phoresy, before the canalization of life cycles around deutonymphal dispersal. The persistence of putatively ancestral traits in schizoglyphids, along with their apparent ecological conservatism in termite nests, indicates the role of environmental stability in preserving modes of life history that otherwise appear extinct. We also provide a key to describe Schizoglyphidae species and discuss the implications of this discovery for understanding the origins of phoresy-related metamorphosis in Astigmata. Full article

The aim of the study was to assess the diversity of termites used in poultry feed in Burkina Faso. Termite samples were collected in eight of the thirteen regions of the country by poultry farmers, then conserved in 70° alcohol. The criteria used by poultry farmers for identifying the termite were also characterised and discussed with farmers in a village where the use of termites as poultry feed is well developed. Morphological identifications were carried out in the laboratory. Twenty species were identified in two families, six subfamilies, and thirteen genera. In the Heterotermitidae family, a single species belonging to the Coptotermitinae subfamily was identified. The rest belonged to the family Termitidae and the subfamilies Amitermitinae, Microcerotermitinae, Macrotermitinae, Nasutitermitinae, and Cubitermitinae. Three species, Microcerotermes fuscotibialis, Megagnathotermes notandus, and Isognathotermes fungifaber, were found for the first time in Burkina Faso. The largest number of species (eleven) was collected in the Cascades region. Poultry farmers are able to recognise eight genera of termites by the shape, size, and colour of the termites; by the termite mounds; and often by the location of the nest. These results may facilitate the promotion of the use of termites as poultry feed in Burkina Faso and West Africa. Full article

Pu’er raw tea, a representative tea with a positive correlation between quality and storage time, has a unique aging process. It can not only reduce the heavy astringent taste of newly produced tea, but also has a complex and fragrant aging aroma. However, the extremely slow natural aging process often takes years or even decades for quality transformation, along with the risk of termite infestation, odor absorption, etc. This study found that UV-C irradiation could significantly accelerate the aging process of tea. It enhanced the richness of the aroma, while reducing astringency and creating a smoother and mellower taste. The results of substances analysis revealed an increase in key aroma compounds such as alcohols, aldehydes and terpenes, and a decrease in catechin and caffeine with UV-C irradiation, which is consistent with the natural aging trend. An efficient and safe rapid aging technology of Pu’er raw tea has been successfully established. Full article

Rocky outcrops are harsh habitats that support specialized organisms and communities, including biocrusts, which play roles in soil stabilization, water retention, and nutrient cycling. Despite their importance, tropical biocrusts, particularly in granite-gneiss formations, remain underexplored. This study examines biocrust composition in a granite-gneiss outcrop in a rural landscape in Southeastern Brazil, identifying microhabitats and analyzing co-occurrence patterns and community structure. We recorded eleven bryophyte species and one diatom species, while six cyanobacteria, three charophytes, and two chlorophytes were identified at the genus level. They were found in shallow depressions, though termite mounds also served as an important microhabitat. The cyanobacterium Scytonema was the most prevalent taxon. The liverwort Riccia weinionis had the highest number of positive co-occurrences, associating with cyanobacteria and algae. Network analysis based on co-occurrence revealed that Scytonema and the mosses Anomobryum conicum and Bryum argenteum were the most connected taxa, crucial for ecological network stability. The moss Bryum atenense acted as a key intermediary, with the highest betweenness centrality—a measure of its role in linking taxa. These findings provide insights into tropical rocky outcrop biocrusts, shedding light on their composition and interactions. Furthermore, the co-occurrence patterns and key taxa connectivity uncovered provide insights into ecosystem stability and can guide ecological restoration strategies. Full article

The discharge of textile effluents containing dyes poses severe environmental risks. This study aimed to develop a $\mathrm{m}\mathrm{a}\mathrm{g}\mathrm{n}\mathrm{e}\mathrm{t}\mathrm{i}\mathrm{c} \mathrm{i}\mathrm{r}\mathrm{o}\mathrm{n} \mathrm{o}\mathrm{x}\mathrm{i}\mathrm{d}\mathrm{e}$–HTM (magnetite–heat-activated termite mound) composite via the coprecipitation method for the adsorption of Basic Blue 41 (BB41) dye from textile wastewater under batch conditions. The $\mathrm{m}\mathrm{a}\mathrm{g}\mathrm{n}\mathrm{e}\mathrm{t}\mathrm{i}\mathrm{c} \mathrm{i}\mathrm{r}\mathrm{o}\mathrm{n} \mathrm{o}\mathrm{x}\mathrm{i}\mathrm{d}\mathrm{e}$–HTM composite was characterized using BET (surface area), XRD (crystalline structure), FTIR (functional groups), and SEM (microstructure) analyses, confirming the successful synthesis of $\mathrm{m}\mathrm{a}\mathrm{g}\mathrm{n}\mathrm{e}\mathrm{t}\mathrm{i}\mathrm{c} \mathrm{i}\mathrm{r}\mathrm{o}\mathrm{n} \mathrm{o}\mathrm{x}\mathrm{i}\mathrm{d}\mathrm{e}$–HTM. Comprising 80% HTM by mass, the composite demonstrates economic viability. Using batch experiments and a Box–Behnken design, the adsorption performance of $\mathrm{m}\mathrm{a}\mathrm{g}\mathrm{n}\mathrm{e}\mathrm{t}\mathrm{i}\mathrm{c} \mathrm{i}\mathrm{r}\mathrm{o}\mathrm{n} \mathrm{o}\mathrm{x}\mathrm{i}\mathrm{d}\mathrm{e}$–HTM for BB41 dye removal from aqueous solutions was evaluated. Optimization of the sorption process revealed that a dosage of 2.6 g/L, a contact time of 47.5 min, a temperature of 60 °C, and an initial dye concentration of 100 mg/L resulted in a BB41 dye removal efficiency of 98%. Additionally, $\mathrm{m}\mathrm{a}\mathrm{g}\mathrm{n}\mathrm{e}\mathrm{t}\mathrm{i}\mathrm{c}$–HTM effectively removed BB41 dye from real wastewater samples, achieving a removal efficiency exceeding 80%, highlighting the improved sorption properties of the modified termite mound. The spent $\mathrm{m}\mathrm{a}\mathrm{g}\mathrm{n}\mathrm{e}\mathrm{t}\mathrm{i}\mathrm{c}$–HTM was easily separated from the treated solution using an external magnet and successfully recovered. Its reusability demonstrated a dye removal efficiency of 78% after four cycles, without compromising its magnetic properties. Overall, the magnetically separable $\mathrm{m}\mathrm{a}\mathrm{g}\mathrm{n}\mathrm{e}\mathrm{t}\mathrm{i}\mathrm{c} \mathrm{i}\mathrm{r}\mathrm{o}\mathrm{n} \mathrm{o}\mathrm{x}\mathrm{i}\mathrm{d}\mathrm{e}$–HTM composite shows significant potential for the treatment of textile wastewater. Full article

Macrotermes barneyi is a typical fungus-growing termite that forms both monogynous (single queen) and polygynous (multiple queen) colonies in nature. This species influences the local soil fertility in part by redistributing nutrients across the landscape in its habitats. However, how the colony structure of M. barneyi affects nutrient cycling and microbial communities within the nest is not well understood. In this study, we compared the physicochemical properties and microbial communities across nest parts between monogynous and polygynous colonies of M. barneyi. Our results showed that the fungus garden is the most nutrient-rich part of the nest, with higher soil moisture, organic matter, ammonium nitrogen, nitrate nitrogen, available sulfur, available potassium, available silicon, and available boron than other nest parts. Notably, the fungus garden in monogynous colonies had higher nitrate nitrogen, available sulfur, and available silicon than those in the polygynous colonies. The microbial α-diversity in the fungus garden was lower than that in other parts of the nest. β-diversity analysis revealed a clear separation of microbial communities between monogynous and polygynous colonies across nest parts. Furthermore, the relative abundance of functional genes associated with “cell cycle control, cell division, and chromosome partitioning” was higher in the fungus garden of polygynous colonies compared to monogynous colonies. Our results suggest that the fungus garden plays a crucial role in maintaining colony stability in M. barneyi colonies. The rapid depletion of nutrients in the fungus garden to sustain the larger population in polygynous colonies likely influences microbial community dynamics and nutrient cycling. Full article

The genetic diversity of 22 colonies of the termite Reticulitermes flaviceps was analyzed in Shaanxi and Sichuan provinces. It was found that the genetic diversity in both regions was quite similar. However, the distribution of genetic variations within the colonies was uneven. The termite colonies showed moderately high genetic diversity, a positive sign for adaptability and survival. The study also revealed a favorable mix of different genetic types within the colonies, indicating a healthy level of genetic variation. However, there was limited genetic exchange among different colonies, leading to noticeable genetic differences. When looking at the genetic structures, the colonies in Shaanxi were quite similar; those in Sichuan showed more variation, and some Sichuan colonies had identical genetic structures to those in Shaanxi. Regarding breeding systems, the colonies in Shaanxi were mainly extended families, meaning they had multiple generations living together. In contrast, most colonies in Sichuan were simple families consisting of just one generation; this difference might be due to the natural, less disturbed environments in Shaanxi, which support more extensive and complex colonies. On the other hand, the urban environments in Sichuan, with their intricate cement structures, made it difficult for termite colonies to expand. Overall, the study highlights the genetic diversity and breeding strategies of R. flaviceps in different environments, providing insights into their adaptability and survival mechanisms. Full article

This research explores the potential of green encapsules uploaded with eucalypt essential oil (EEOs) in enhancing their functionality and application in pest control, focusing on suitable ecotype selection from King Abdulaziz University (KAU) campus, Hada Al-Sham (HAS) village, and Briman district as well as optimizing extraction processes. Eucalypt hybrids’ leaves were collected from three different sites, and the EEOs were extracted using microwave-assisted steam distillation (MASD) and electric steam distillation (ESD) techniques. The physical and chemical properties of the EEO were determined. The identification of volatile chemical ingredients in the resulting EEOs was conducted using GC/MS after saponification and methylation procedures, and the ingredients were compared to those obtained from Eucalyptus globulus Labill, the ideal species containing the 1,8-cineol, the principal compound in its essential oil. The 1,8-cineole was found to be the major chemical constituent of the EEOs all over the two extraction methods, regardless of the ecotypes examined, and was interfered with other minor components such as 3-carene, α-pinene, α-myrcene, D-limonene, and α-terpinene. Eucalypt ecotypes grown at Hada Al-Sham village had the highest cineole content (59.29%) among the other sites studied. Compared to the ESD technique, MASD showed much promise because it is simple, facile, more ecofriendly and cost-effective, it kept oils true to their original form, and it allows to warm larger machines and spaces. The polymeric encapsules of either guar gum crosslinked by borax or sodium alginate crosslinked by calcium chloride were fabricated. Moreover, a bioassay screening of the encapsules uploaded with 1,8-cineole was evaluated against termite infection. The encapsules were found to be versatile tools with a wide range of applications; in particular, the alginate encapsules displayed superior characteristics. Furthermore, regardless of the encapsule type and the exposure duration, the mortality (%) of the insects was exceeded significantly for the high cineol concentrations compared to the lower ones for both alginate-based encapsules (ABEs) and guar gum-based encapsules (GGBEs). The higher the cineol concentrations, the higher the mortality percent of the termites. This finding can be attributed to the rapid toxic effect of the cineol compound at higher concentrations. Full article

Amid global decarbonization mandates, urban distribution networks (UDNs) face escalating voltage volatility due to proliferating distributed energy resources (DERs) and emerging loads (e.g., 5G base stations and data centers). While virtual power plants (VPPs) and network reconfiguration mitigate operational risks, extant methods inadequately model load flexibility and suffer from algorithmic stagnation in non-convex optimization. This study proposes a proactive voltage control framework addressing these gaps through three innovations. First, a dynamic cyber-physical load model quantifies 5G/data centers’ demand elasticity as schedulable VPP resources. Second, an Improved Termite Life Cycle Optimizer (ITLCO) integrates chaotic initialization and quantum tunneling to evade local optima, enhancing convergence in high-dimensional spaces. Third, a hierarchical control architecture coordinates the VPP reactive dispatch and topology adaptation via mixed-integer programming. The effectiveness and economic viability of the proposed strategy are validated through multi-scenario simulations of the modified IEEE 33-bus system (represented by 12.66 kV, it is actually oriented to a broader voltage scene). These advancements establish a scalable paradigm for UDNs to harness DERs and next-gen loads while maintaining grid stability under net-zero transitions. The methodology bridges theoretical gaps in flexibility modeling and metaheuristic optimization, offering utilities a computationally efficient tool for real-world implementation. Full article