Search Results (531)

Rhododendron tomentosum Harmaja is a marsh plant known for its high content of bioactive components, including essential oil, flavonoids, and phenolic acids. In the current work, the effects of cultivation mode (agar, liquid stationary, shake flask, and temporary immersion) and experiment duration (30, 60, and 90 days) on the growth and contents of non-volatile phenolics in Rhododendron tomentosum microshoots were investigated. Agar and liquid stationary cultures provided the highest dry biomass yield per liter, but their dry weight productivities per day were the lowest among the tested systems. Agitated and temporary immersion cultures, on the other hand, were the most productive in terms of fresh and dry biomass yield per day. LC-DAD-ESI-MS analysis of extracts from microshoots and wild-grown plants revealed similarities in phenolic composition: in both cases, the presence of catechin, chlorogenic acid, and flavonoids of flavonol type (mainly glycosides of quercetin and myricetin) was confirmed. The qualitative composition of the phenolic fraction was not affected by experiment duration and cultivation mode. As determined by HPLC analysis, shake flask and temporary immersion cultures were characterized by the highest phenolic contents: up to 37.5 and 26 mg/g dry weight, respectively. The maximum productivities of the above systems were equal to 18 and 13.5 mg/L/d, respectively. Full article

Mg-Li-based dissoluble metal is a promising material for preparing dissoluble magnesium alloy wires. However, there are few reports on the development of Mg-Li dissoluble magnesium alloy wires so far. In this paper, the mechanical properties and dissoluble properties of as-drawn and annealed LA141-0.5Cu wires were investigated in detail. It was found that the tensile strength of the LA141-0.5Cu wires decreased from 160 MPa to 127 MPa and the elongation increased from 17% to 22% after annealing. The difference in corrosion rates (93 °C/3% KCl solution) between the as-drawn wires and annealed wires is not significant, with values of 5.1 mg·cm⁻²·h⁻¹ and 4.5 mg·cm⁻²·h⁻¹, respectively. This can be explained as follows: after annealing, the number of dislocations in the wire decreases, the strength decreases, and the plasticity increases. The reason why the wires have a significant corrosion rate is that there is a large potential difference between the Cu-containing second phase and the magnesium matrix, which forms galvanic corrosion. The decrease in dislocation density after annealing leads to a slight reduction in the corrosion rate of the wires. This work provides a qualified material for fabricating temporary blocking knots for the exploitation of unconventional oil and gas resources. Full article

As is already known, some currently stable landslides may have been activated in the past along a pre-existing sliding surface and reached the residual strength there, as a consequence of high-cumulative displacements. After a fairly long period of quiescence, these landslides can reactivate due to a temporary increase in destabilising forces capable of mobilising the residual strength along the same sliding surface again. Some recent studies have suggested that, under certain conditions, the strength mobilised upon reactivation may slightly exceed the residual value and then decay towards the latter as the displacement progresses. Regarding this matter, many previous studies have hypothesised that some geotechnical variables could affect the recovered strength more significantly: the length of the ageing time, the vertical stress, the stress history, and the speed with which the reactivation occurs. The aim of this research is to confirm whether such recovery of strength upon reactivation is possible and which geotechnical parameters have the greatest influence on the process. To this end, laboratory tests were carried out with the Bromhead ring shear apparatus on normally consolidated saturated samples of both natural soils and clays provided by industry (bentonite and kaolin). The coupling effect of the ageing time, the vertical stress, and the reactivation speed on the mobilised strength upon reactivation were investigated, starting from a pre-existing residual state of these samples. Within the limits of this research, the results seem to confirm that all three geotechnical variables are influential, with a greater impact on the reactivation speed and, subordinately, on the ageing time for long quiescence periods. Therefore, it is concluded that a quiescent landslide could show a reactivated strength slightly higher than the residual value if the destabilising action could arise with a certain rapidity. Conversely, if the destabilising action occurs very slowly, the mobilised strength could correspond to the residual value. The experimental results of this research may find some application in the design of strengthening works for a stable quiescent landslide that could experience a fairly rapid increase in destabilising actions, such as in the case of seismic stress, morphological modification of the slope, or a rising water table. Full article

The addition of battery storage to solar plants enhances the ability of those plants to deliver electricity during high-value periods. However, the value proposition of storage improves over time due to falling battery costs and increasing volatility in electricity prices, making it unclear when storage adoption should occur. In this work, we consider a 100 MW solar plant constructed in the year 2022 and build a techno-economic model to determine the optimal system design and timing of storage additions in four locations (CAISO, NYISO, ERCOT, and PJM). We find that the optimal time to add storage is 5–10 years after solar plant construction and that the optimal storage quantity is much higher than the amount selected if storage is included during the initial plant construction. Additionally, the model suggests significant upscaling in inverter capacity, allowing storage to deliver electricity during brief high-price periods. We also consider the effects of temporary and permanent subsidies for batteries, showing that a long-term subsidy encourages economically optimal delays in storage adoption. Full article

The sustainable utilization of forest biomass for bioenergy production is increasingly challenged by the variability and unpredictability of raw material availability. These challenges are particularly critical in regions like Central Portugal, where seasonality, dispersed resources, and wildfire prevention policies disrupt procurement planning. This study investigates two flexibility strategies—dynamic network reconfiguration and operations postponement—as policy relevant tools to enhance resilience in forest-to-bioenergy supply chains. A novel mathematical model, the mobile Facility Location Problem with dynamic Operations Assignment (mFLP-dOA), is proposed and solved using a scalable matheuristic approach. Applying the model to a real case study, we demonstrate that incorporating temporary intermediate nodes and adaptable processing schedules can reduce costs by up to 17% while improving operational responsiveness and reducing non-productive machine time. The findings offer strategic insights for policymakers, biomass operators, and regional planners aiming to design more adaptive and cost-effective biomass supply systems, particularly under environmental risk scenarios such as summer operation bans. This work supports evidence-based planning and investment in flexible logistics infrastructure for cleaner and more resilient bioenergy supply chains. Full article

Background/Objectives: Employment instability is increasingly recognized as an organizational stressor, yet its combined effect on nurse burnout, humanized care, and work engagement is poorly quantified. This study investigates those relationships and tests a serial mediation model linking contract instability, burnout, humanization, and engagement in Spanish hospital nurses. Methods: A nationwide cross-sectional survey was completed by 400 fixed-term nurses between March and May 2025. The data included demographics, number of contracts signed during 2024, and scores on the Maslach Burnout Inventory (MBI), Utrecht Work Engagement Scale (UWES), and Health Professionals’ Humanization Scale (HUMAS). Spearman coefficients described the bivariate relations. Results: Burnout correlated positively with both contract count (r = 0.42, p = 0.039) and years of experience (r = 0.74, p = 0.040). Work engagement was inversely associated with instability (r = –0.62, p = 0.018). Humanized care was strongly and negatively related to burnout (r = –0.61, p = 0.032), particularly in sociability and self-efficacy dimensions. Discussion: Contractual precarity elevates burnout, erodes perceptions of humanized care, and, through this erosion, suppresses nurse engagement. Stabilizing workforce arrangements and strengthening empathy-centered skills may mitigate these effects and foster a socially sustainable nursing workforce. Full article

Clostridioides difficile is a Gram-positive bacterium recognized for its ability to produce toxins and form spores. It is mainly accountable for the majority of instances of antibiotic-related diarrhea. Background. Bacterial persister represent a minor fraction of the population that shows temporary tolerance to bactericidal agents, and they pose considerable medical issues because of their link to the rise of antibiotic resistance and challenging chronic or recurrent infections. Our previous research has shown a persister-like phenotype associated with treatments that include pefloxacin. Nonetheless, the mechanism is still mostly unclear, mainly because of the difficulty in isolating this small group of cells. Objectives. To enhance the understanding of C. difficile persister cells, we made an enrichment and characterization of these cells from bacterial cultures during the exponential phase under pefloxacin treatment and lysis treatment. Results. We demonstrate the appearance of cells with lower metabolism and DNA damage. Furthermore, we noted the participation of toxin–antitoxin systems and Clp proteases in the generation of persister cells. Conclusions. This work demonstrates the formation of C. difficile persister cells triggered by a lethal concentration of pefloxacin. Full article

An adequate food supply is a core issue for sustainable development worldwide. Amid greater instability in the food supply triggered by more armed conflicts, trade disputes, and climate change, a decline in grain cultivation area still plagues many regions. China, a major food producer globally, is a case in point. The truth is that at the moment, the formulation and implementation of policies as well as academic discussions regarding this issue are predominantly based on the sown area of grains, overlooking the fundamental role co-played by population, yield efficiency, and sown area in determining food supply. Furthermore, the commonly used indicator, the non-grain cultivation rate, fails to directly reflect the impact of the phenomenon on the grain supply. To address these gaps, this study introduces trend-change detection and factor-contribution analysis, uses long-term grain sown area data to identify regions with significant grain retreat, and quantifies the relative influence of population shifts, crop yield improvements, and sown area changes on food supply. Key findings include the following: China’s total grain production maintained steady growth from 2003 to 2023, far exceeding conventional food security thresholds. Temporary reductions in grain sown area (2015–2019, 2021–2022) were offset by rising yields, with no substantial decline in supply. Twelve provinces/municipalities, Beijing, Shanghai, Zhejiang, Fujian, Guangdong, Guangxi, Guizhou, Shaanxi, Ningxia, Sichuan, Chongqing, and Hainan, exhibited substantial declines in grain plantation. However, Sichuan and Shaanxi achieved counter-trend growth in food supply, while Ningxia and Guizhou experienced frequent fluctuations. The sown area was not always the dominant factor in per capita grain availability. Yield increases neutralized cropland reduction in Sichuan, Shaanxi, Guizhou, and Ningxia, whereas population inflows outweighed the sown area effect in the other eight provinces. The study concludes that China’s grain cropland reduction has not yet posed a threat to national food security. That said, the spatial concentration of these affected regions and their ongoing output reductions may raise domestic grain redistribution costs and intensify inter-regional conflicts over cropland protection. Meanwhile, population influx plays a similarly important role to that of grain plantation decline in the grain supply. Considering that, we believe that more moderate measures should be adopted to address the shrinkage of grain planting areas, with pre-set food self-sufficiency standards. These measures include, but are not limited to, improving productivity and adopting integrated farming. Methodologically, this work lowers distortions from normal annual cropland fluctuations, enabling more precise identification of non-grain production zones. By quantifying the separate impacts of population, crop yield, and sown area changes, it supplements existing observations on grain cropland decline and provides better targeted suggestions on policy formulation and coordination. Full article

This review examines the application of the geophysical methods for Transportation Infrastructure Slope Monitoring (TISM). In contrast to existing works, which address geophysical methods for natural landslide monitoring, this study focuses on their application to infrastructure assets. It addresses the key aspects regarding the geophysical methods most employed, the subsurface properties revealed, and the design of monitoring systems, including sensor deployment. It evaluates the benefits and challenges associated with each geophysical approach, explores the potential for integrating geophysical techniques with other methods, and identifies the emerging technologies. Geophysical techniques such as Electrical Resistivity Tomography (ERT), Multichannel Analysis of Surface Waves (MASW), and Fiber Optic Cable (FOC) have proven effective in monitoring slope stability and detecting subsurface features, including soil moisture dynamics, slip surfaces, and material heterogeneity. Both temporary and permanent monitoring setups have been used, with increasing interest in real-time monitoring solutions. The integration of advanced technologies like Distributed Acoustic Sensing (DAS), UAV-mounted sensors, and artificial intelligence (AI) promises to enhance the resolution, accessibility, and predictive capabilities of slope monitoring systems. The review concludes with recommendations for future research, emphasizing the need for integrated monitoring frameworks that combine geophysical data with real-time analysis to improve the safety and efficiency of transportation infrastructure management. Full article

As humanity embarks on interplanetary exploration and envisions future colonies beyond Earth, understanding the impact of extreme environments on life becomes paramount. Among these factors, the hypomagnetic field (HMF)—a condition where the protective geomagnetic field is absent—remains poorly understood, especially regarding its effects on (micro)organisms. To our knowledge, this is the first study to examine how short-term exposure to an HMF (24 h to 7 days) affects the growth of three different microorganisms, Saccharomyces cerevisiae, Acidithiobacillus ferrooxidans, and Lactobacillus plantarum, using a specialized hypomagnetic chamber and advanced spectrophotometric analysis. We demonstrate significant growth inhibition in S. cerevisiae (23%) and A. ferrooxidans (68%), with L. plantarum remaining unaffected. This inhibitory effect appears reversible, diminishing as organisms return to normal geomagnetic conditions. These findings reveal that the HMF acts as a temporary environmental stressor, underscoring the need for deeper exploration of its biological effects. Our work sets the stage for further research into how the space environment may shape microbial ecosystems critical to future human endeavors in space. Full article

Background/Objectives: After years of work in the field of aortic arch surgery, the technique has evolved, making this procedure relatively safe, with lasting results. Due to the increasing long-term survival and overall aging of the patient population, more patients require aortic arch reoperation. In the present study, the safety of aortic arch reoperations was analyzed in the long term, focusing on risk factors for mortality. Methods: Between 1999 and 2023, 108 patients were included in our study who underwent reoperation on aortic arch after prior operation on the aorta, the aortic valve, or a combination of both. The exclusion criteria were being aged under 18 years and transcatheter aortic valve implantation as a previous intervention. The principal outcome was the incidence of mortality, and additional outcomes of interest included cardiac re-reoperation, bleeding, a new aortic type B dissection, infective endocarditis, readmission due to a cardiac cause, coronary intervention and neurovascular complications, pacemaker implantation, and temporary mechanical circulatory support. Results: The mean age was 56 ± 14 years, and 75% (81/108) of patients were male. In our study, we found age (p ≤ 0.01) and history of coronary artery disease (p = 0.01) to be preoperative risk factors for adverse outcomes. The mean time between the index operation and reoperation was 6.84 years (1.61–14.94). Indications for reoperation included dilatation (HR = 0.49, p = 0.05), rupture or false aneurysm (HR = 2.08, p= 0.08), dissection (HR = 1.41, p = 0.30), and endocarditis (HR = 1.49, p = 0.41). A main risk factor was the need for a salvage reoperation (p ≤ 0.01). Also, a longer operation (p = 0.04), cardiopulmonary bypass (p ≤ 0.01), and ventilation time (p ≤ 0.01), bleeding complications (p ≤ 0.01), and requiring temporary mechanical circulatory support (p = 0.04) were linked to higher mortality. The overall survival was 82% after 1 year, 73% after 5 years, and 56% after 10 years. In the multivariate Cox regression analysis, age (HR = 1.04, p ≤ 0.01), the need for a salvage operation (HR = 5.38, p = 0.01), a prolonged ventilation time (HR = 1.08, p = 0.04), and bleeding complications (HR = 3.76, p = 0.03) were associated with higher mortality. In the ROC analysis, an age over 57.5 years was associated with significantly lower overall survival (p ≤ 0.01). Conclusions: Aortic arch reoperations can be performed with acceptable long-term outcomes, but perioperative factors significantly influence early mortality. Salvage operations, bleeding complications, and prolonged ventilation were strong predictors of adverse outcomes. Older age, particularly >57.5 years, was independently associated with increased mortality risk. Full article

The rapid advancement of modern megapolises has led to a dearth of surface space, and, in response, engineers have begun to trial substitutes below ground level. Shafts are generally used to provide temporary access and permanent work to the subsurface for tunnelling, as well as for lifts or ventilation purposes. In urban areas, one important design issue is the prediction of the excavation-induced displacements by open caisson shaft construction. Settlements and ground movements associated with open caisson shafts are influenced by the choice of construction method, soil composition, and excavation geometry. Compared with other geotechnical construction events, for instance, tunnelling, the literature relating to the ground deformations induced from open caisson shafts are comparatively limited. This review offers an evaluation of several case studies that utilize experimental and computational modeling techniques to provide clearer insights into earth pressure distribution and induced surface and subsurface soil displacements, as well as the associated ground deformations during open caisson shaft construction. The modeling test results are compared to the state of the practice ground deformation prediction theories and measured results from field monitoring data. Findings indicate that the lateral earth pressure distribution aligns closely with the theoretical predictions based on Terzaghi’s and Berezantzev’s models, and lateral earth pressure diminishes gradually until the onset of active wall displacement. Current modeling techniques generally fail to properly represent in situ stress states and large-scale complexities, emphasizing the need for hybrid approaches that combine physical and numerical methodologies. In future studies, modern approaches, including artificial intelligence (AI) monitoring (e.g., PINNs, ACPP), multi-field coupling models (e.g., THMC), and transparent soil testing, hold profound potential for real-time prediction, optimization, and visualization of soil deformation. Numerical–physical coupling tests will integrate theory and practice. Improving prediction reliability in complicated soil conditions such as composite and heterogenous strata using different modeling techniques is still unclear, and further investigation is therefore needed. Full article

The chicken chorioallantoic membrane (CAM) model is an embryonic blood capillary system considered a suitable “in vivo” model for studying the irritation effect of plant extracts on the vascular system, including impacts on hemostasis, hyperemia, hemorrhage, and coagulation. The main aim of the present work was to investigate the irritation effects of different concentrated alcohol extracts of Ginkgo biloba L. (GBE) leaves on the blood vessels of the CAM model during early embryogenesis, evaluated using the Luepke scoring system. The antioxidant properties of GBE were assessed using DPPH radical scavenging and the FRAP method, alongside HPLC-DAD analysis to confirm the presence of major therapeutically relevant metabolites, revealing a strong therapeutic potential of the extract. On embryonic day 9, different concentrations of GBE as well as the controls (saline solution and 30% ethanol) were applied to the CAM surface. Vascular changes were observed immediately after application, with vasoconstriction leading to the temporary “disappearance” of blood vessels. At 30 s post-application, all GBE concentrations and ethanol induced hyperemia and mild hemorrhage, which gradually diminished over time. No changes were observed with saline application. The extent of morphometric changes in the vessels was also influenced by the concentration of GBE used. Concentrations of 20% and 30% GBE induced vasoconstriction. Lower concentrations of GBE induced vasodilation, with maximum values recorded after 240 s for 1% and 15% GBE. The results of this study may help to better characterize the vascular effects of natural Ginkgo biloba under in vivo conditions and promote greater interest in the use of alternative animal models in pharmacological and biomedical research. Full article

To investigate the performance degradation of emulsified asphalt cold recycled mixtures (CRM) during service, this study selected a 10 km section of the cold recycled layer (CRL) from the Changjiu Expressway reconstruction project as the research subject. The deterioration patterns of key pavement performance indicators—including the Pavement Condition Index (PCI), Riding Quality Index (RQI), Rutting Depth Index (RDI), and Pavement Structure Strength Index (PSSI)—were analyzed in relation to cumulative equivalent axle loads over a 7-year service period. Concurrently, comparative evaluations were conducted on the mechanical properties, water stability, high-temperature performance, low-temperature crack resistance, and fatigue characteristics between in-service and laboratory-prepared emulsified asphalt CRM. The results demonstrate that after seven years of service, the emulsified asphalt cold recycled pavement maintained excellent performance levels, with PCI, RQI, RDI, and PSSI values of 92.6 (excellent), 90.1 (excellent), 88.5 (good), and 93.4 (excellent), respectively. Notably, while the indirect tensile strength and unconfined compressive strength of the CRL increased with prolonged service duration, other performance metrics—including the tensile strength ratio, shear strength, fracture work, and fracture energy—exhibited an initial improvement followed by gradual deterioration. Additionally, increased traffic loading during service led to a reduction in the residual fatigue life of the CRM. Interestingly, the study observed a temporary improvement in the fatigue performance of CRM during the service period. This phenomenon can be attributed to three key mechanisms: (1) continued cement hydration, (2) secondary hot compaction effects, and (3) diffusion and rejuvenation between fresh and aged asphalt binders. These processes collectively contributed to the partial recovery of aged asphalt strength, thereby improving both the mechanical properties and overall road performance of the CRM. The findings confirm that cold recycled pavements exhibit remarkable durability and maintain a high service level over extended periods. Full article

Background/Objectives: Drug shortages are a serious issue affecting health systems worldwide, determined by multiple causes including supply issues, regulatory limitations, and market distortions. The possible repercussions on patients may impair therapeutic efficacy. Despite numerous actions being implemented by regulatory authorities, including market monitoring, export restrictions, and temporary regulation mitigations, few instruments have been made available to help health operators find marketed alternatives to unavailable products. The aim of this work was to create an algorithm to find equivalent or alternative medicinal products available in a certain pharmaceutical market. Algorithm development and validation were performed using the medicinal products marketed in Italy. Methods: First, a newly assembled code, describing the active pharmaceutical ingredient by its Anatomical Therapeutical Chemical (ATC) code, and its dosage form by the European Directorate for the Quality of Medicines & HealthCare (EDQM) Standard Terms, was attributed to each marketed medicinal product. Then, the algorithm was set up to identify its possible equivalents or alternatives by assigning a score quantifying differences in Defined Daily Dose (DDD) per presentation unit and in characterizing Standard Terms. Results: The algorithm was validated on a randomized sample of medicinal products, proving to be able to identify appropriate equivalents or alternatives; moreover, it was tested in real conditions by submitting a survey to health professionals, who found this product to be reliable and useful. Conclusions: The developed algorithm may be employed as a rational tool to help health operators find solutions to drug shortages. This work highlighted some limits of the current ATC attribution that should be addressed by the competent authorities. Full article