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Life
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Natural Substances in Nutrition and Health of Animals: 2nd Edition
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Natural Substances in Nutrition and Health of Animals: 2nd Edition

A special issue of Life (ISSN 2075-1729). This special issue belongs to the section "Animal Science".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 126

Special Issue Editors

Dr. Karaffová Viera

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Guest Editor
Department of Morphological disciplines, The University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia
Interests: probiotic bacteria; innate immunity; molecular biology; campylobacteriosis; PCR methods
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Zuzana Ševčíková

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Guest Editor
Department of Morphological Disciplines, The University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia
Interests: oncology; histology; imunochemistry; cytology
Special Issues, Collections and Topics in MDPI journals
Dr. Viera Almášiová

E-Mail Website
Guest Editor
Department of Morphological Disciplines, The University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia
Interests: histology; ultrastructure of tissues and organs; electron microscopy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleague,

In recent years, there has been a growing interest, especially from a dietary point of view, in the consumption of healthy animal products. At the same time, emphasis has been placed on the use of alternative methods of prevention of the emergence and spread of diseases in animal husbandry. The quality of animal products depends, among other factors, mainly on feed or other additives. In the past, antibiotics have been widely used in animal husbandry and also as effective growth stimulators. Their frequent and excessive use over time has led to the development of severe resistance to many types of pathogens. Equally serious concern has been the increase in the amount of residual substances in animal meat, which poses a direct threat to the health of humans as consumers. A promising alternative in this regard appears to be the application of various natural substances or bioactive ingredients such as probiotic bacteria, enzymes, organic acids, and plant ingredients, as well as other lesser known substances. It has been found that these substances can have a beneficial effect on animal organisms, which ultimately leads to improved health and, consequently, to an increase in the quality of animal products.

This Special Issue offers an open access forum that aims at bringing together a collection of review and original research articles that address a deeper understanding of this expanding field, such as in regard to the use of substances of natural origin in animal feed or the use of different animal models. In this regard, we welcome contributions that cover the effects of natural substances on animals in various specific areas. We look forward to providing new insights in the extended field of animal nutrition and health, especially in regard to how it directly impacts human health.

Dr. Karaffová Viera
Prof. Dr. Zuzana Ševčíková
Dr. Viera Almášiová
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Life is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • natural products
  • organic acids
  • probiotics, prebiotics, and synbiotics
  • plant extracts
  • animal nutrition
  • animal physiology
  • animal embryology
  • animal reproduction
  • animal models

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Related Special Issue

Published Papers (1 paper)

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Research

14 pages, 795 KiB  
Article
Susceptibility of Lactobacillaceae Strains to Aminoglycoside Antibiotics in the Light of EFSA Guidelines
by Marta Dec, Klaudia Herman-Ostrzyżek, Aldert Zomer and Renata Urban-Chmiel
Life 2025, 15(5), 732; https://doi.org/10.3390/life15050732 (registering DOI) - 30 Apr 2025
Abstract
Lactobacillaceae is a large family of bacteria from which probiotic strains often originate. Microorganisms used as feed additives in the EU must meet a number of formal criteria, some of which concern antimicrobial susceptibility. In this study, we determined the susceptibility of 19 [...] Read more.
Lactobacillaceae is a large family of bacteria from which probiotic strains often originate. Microorganisms used as feed additives in the EU must meet a number of formal criteria, some of which concern antimicrobial susceptibility. In this study, we determined the susceptibility of 19 reference strains and 121 wild-type strains of Lactobacillaceae to aminoglycoside antibiotics using the broth microdilution method based on the ISO 10932:2010/IDF 223:2010 standard. Strains were categorized as resistant or susceptible according to European Food Safety Authority (EFSA) guidelines. Resistance genes were detected by whole genome sequence (WGS) analysis or by PCR. The MICs read after 48 h of incubation showed that 36.8% of reference strains were resistant to kanamycin, 26.3% to streptomycin, and 5.3% to gentamicin, with no aminoglycoside resistance genes detected in any genome. As many as 93.2% of field isolates of Ligilactobacillus salivarius, 85% of Ligilactobacillus agilis, and 58.8% of Lactiplantibacillus plantarum were classified as resistant to kanamycin, with the aac(6)-Ie-aph(2)-Ia gene detected only in two isolates. In six of 12 streptomycin-resistant strains, the ant(6)-Ia gene was identified, which usually coexisted with the spw gene. Three isolates with high neomycin MICs harbored the ant(4′)-Ia gene. In Lactobacillus gallinarum strain LMG 9435, characterized by streptomycin MIC value > 1024 µg/mL, a potential resistance-causing mutation in the rpsL gene (Lys56 ® Arg) was detected. The results of the study indicate that some genera of Lactobacillaceae, in particular L. salivarius and L. agilis, exhibit natural resistance to aminoglycoside antibiotics, mainly kanamycin. Therefore, there is a need to update the EFSA guidelines on antimicrobial susceptibility testing of Lactobacillaceae, so that strains lacking resistance genes and/or chromosomal mutations are not considered to be resistant. Full article
(This article belongs to the Special Issue Natural Substances in Nutrition and Health of Animals: 2nd Edition)
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