Search Results (903)

Swine manure, a heterogeneous livestock waste composed of solid and liquid excreta, can be sustainably converted through Chemical Looping Gasification (CLG) to produce syngas and bioenergy. Integrated with CO₂ capture, the process enables high-purity hydrogen generation and offers a potential route toward net-negative carbon emissions. The experimental campaign was conducted at 900 °C in a continuously operated 0.5 kW_th CLG unit consisting of two interconnected fluidized bed reactors (fuel and air). Ilmenite was employed as the oxygen carrier to provide the oxygen required for gasification. This study focuses on the gasification of raw swine manure, comprising both solid and liquid fractions. The solid fraction was introduced via a screw feeder, while the liquid fraction was simulated by injecting an ammonia–water solution as gasifying agents (water or ammonia + water). The effect of the liquid fraction on syngas composition, carbon conversion, and nitrogen species (N₂, NH₃, N₂O, NO₂, and NO) was evaluated at ammonia concentrations typical of swine manure (800–5600 mg/L). Results showed an average syngas composition for solid and liquid fraction feeding of ~31% CO₂, 20% CO, 41% H₂, 7% CH₄, and 0.5% C₂ hydrocarbons, with 91–96% carbon conversion. Benzene and naphthalene dominated the tar compounds. CO₂ capture potential reached 60%, with nitrogen mainly converted to N₂. Full article

Pig somatic cell nuclear transfer (SCNT) has valuable applications in agriculture, biomedicine, and life sciences, yet low cloning efficiency remains a major constraint limiting its application. To systematically investigate factors related to the production efficiency of pig cloning, this study conducted a retrospective analysis of 367,701 SCNT embryos transferred into 2019 surrogate sows over five years, focusing on breeds of donor cells, the season of embryo transfers, and the number of embryos transferred per surrogate. Our data demonstrate that the genetic background of donor cells is a critical determinant. SCNT embryos generated by wild-type (WT) Pietrain and Duroc pigs yielded significantly higher cloning efficiencies compared to those from Large White and Yorkshire pigs. This breed-specific influence was also observed with genetically modified (GM) donor cells. Nevertheless, within the GM groups, GM-Duroc and GM-Yorkshire showed superior efficiency compared to GM-Large White and GM-Bama. Furthermore, Summer was identified as the least favorable season for embryo transfer, with significantly lower pregnancy rates, delivery rates, and cloning efficiency compared to the other seasons. Importantly, we established that transferring 100–150 embryos per recipient optimized cloning efficiency, significantly outperforming groups receiving higher embryo numbers without compromising pregnancy rates, delivery rates, or average litter sizes. Our findings provide valuable guidance for optimizing large-scale SCNT protocols in swine. Full article

Enteric infections remain a major health and economic challenge in swine production, with outcomes determined not only by pathogen virulence but also by the complex interplay between host genetics, immune competence, and the intestinal microbiota. This review synthesises current knowledge on host–pathogen genomic interactions in pigs, with a focus on resilience mechanisms against enteric diseases in swine. For this purpose, 103 articles were used as information sources, retrieved through structured keyword searches in PubMed. The review first addresses host genetic factors, highlighting genomic variants and quantitative trait loci associated with resistance or resilience to viral and bacterial pathogens such as porcine epidemic diarrhoea virus (PEDV) or Escherichia coli. Next, the key factors of the immune system to confer protection are also reviewed, emphasising the role of innate and adaptive responses in controlling each pathogen and disclosing the contribution of regulatory networks that balance pathogen clearance. Finally, the last section of the review is devoted to exploring current knowledge in the involvement of the microbiota in resilience against enteric pathogens, mostly, but not exclusively, enteric bacteria. In this sense, competitive exclusion is a concept which has gained attention in recent years. The review pinpoints and discusses the state of the art about how the microbial community provides colonisation resistance, shapes immune development, and influences pathogen fitness within the intestinal niche. As final perspectives, the review explores future drivers in the genetic immune and microbiota resistance. By bridging host genomic data with functional insights into immunity and microbial ecology, this review underscores the potential of multi-omics approaches to enhance resilience against enteric infections in pigs and advance sustainable swine health management. Full article

The indiscriminate and excessive use of antimicrobial agents in livestock production is a major driver of antimicrobial resistance (AMR), thereby posing a grave threat to global public health. Although several surveillance studies have documented antimicrobial resistance patterns of swine-derived E. coli in different regions of China, comprehensive investigations integrating multilocus sequence typing (MLST), resistance determinants, and virulence gene profiles have remained scarce for central China, particularly Hubei province, since 2018. This study investigated the prevalence of antibiotic resistance, and molecular epidemiology of E. coli isolated from swine farms in Hubei province, China, while simultaneously analyzing their clonal and genetic diversity. A total of 148 E. coli isolates were collected from porcine sources in central China, revealing distinct regional variations in genetic diversity. Multilocus sequence typing (MLST) analysis identified 38 sequence types (STs) distributed across 7 clonal complexes (CCs) and several unassigned clones. ST46 emerged as the predominant sequence type (19.6% prevalence), followed by ST23 and ST10. Antimicrobial susceptibility testing demonstrated 100% resistance to lincosamides and sulfonamides, with all isolates exhibiting multidrug resistance (MDR) to antimicrobial classes. Genetic characterization detected 16 resistance determinants, with individual isolates carrying 5–7 resistance genes on average. The resistance profile included seven β-lactamase genes: blaTEM (61.5%), blaCTX-M-1G (57.4%), blaDHA (46.6%), blaSHV (39.2%), blaCTX-M-9G (24.3%), blaOXA (13.5%), and blaCMY-2 (1.4%); and eight aminoglycoside-modifying enzyme genes, including polymyxin resistance gene mcr-1 (7.4%). Virulence factor screening through PCR detected nine associated genes, with EAST1, fyuA, STa, K88, STb, Irp2, and LT-1 present in 95.3% of isolates, while K99 and 987P were absent in all specimens. This investigation documents alarmingly high antimicrobial resistance rates in swine-derived E. coli populations while elucidating their genetic diversity. The findings suggest that intensive antibiotic use in porcine production systems has driven the evolution of extensively drug-resistant bacterial isolates. These results emphasize the urgent need to implement antimicrobial stewardship programs in livestock management to mitigate AMR proliferation. Full article

Porcine reproductive and respiratory syndrome virus (PRRSV) remains a major cause of economic loss in the swine industry, and highly pathogenic variants such as NADC34-like PRRSV highlight the need for antiviral strategies that complement vaccination. In this field study, we evaluated the efficacy of AlimenWOW, a rottlerin–lipid formulation, in grower–finisher pigs under commercial conditions using AI-based respiratory monitoring. A total of 2000 pigs were assigned to four groups: AlimenWOW G1 (PRRSV-stable source farm), AlimenWOW G2 (PRRSV-unstable source farm), Control 1 (antibiotic), and Control 2 (antipyretic). Respiratory Health Status (ReHS) and a derived Clinical Cough Index (CCI = 100 − ReHS) were continuously recorded with SoundTalks^®, and oral fluid PRRSV load, serology, clinical outcomes, and productivity were assessed over 4 weeks. AlimenWOW G2 showed a marked improvement in ReHS from severely compromised baseline values to levels comparable with healthy status, while both control groups remained low; CCI was significantly lower in AlimenWOW G2 than in controls from day 14 onward (p ≤ 0.0001). AlimenWOW treatment was associated with reduced PRRSV titers in oral fluid, lower mortality and wasting rates, and improved feed conversion with lower feed costs compared with controls. These findings indicate that AlimenWOW, integrated with AI-based acoustic monitoring, can improve respiratory health and mitigate PRRSV-associated clinical and economic losses, supporting its use as a complementary tool in PRRSV control programs. Full article

Air emissions from livestock farming, particularly ammonia (NH₃) and particulate matter (PM_2.5 and PM₁₀), constitute a major environmental and occupational health concern. The aim of this work was to assess the compliance with the Verification of Environmental Technologies for Agricultural Production (VERA) protocol in livestock emission monitoring studies and to propose the Adherence VERA Index (AVI) as a novel quantitative tool for standardizing methodological evaluation. A literature search was conducted in PubMed and Scopus, identifying 26 eligible studies published between January 2012 and June 2025. Data were extracted on farm characteristics, analytical methods, environmental variables, and emission outcomes, and evaluated across the five VERA protocol domains. The review revealed substantial methodological heterogeneity and overall suboptimal compliance with the VERA protocol, with frequent deficiencies in the reporting of key parameters such as ventilation rate, sampling strategy, and emission estimation methods. In this context, the AVI, by condensing core VERA requirements into a concise and operational metric, may facilitate protocol uptake and improve reporting compliance compared with the full VERA framework. Notably, several studies reported NH₃, PM_2.5 and PM₁₀ concentrations exceeding occupational and environmental exposure thresholds, particularly in swine and poultry farms, highlighting critical risks to workers’ health. These findings underscore the need for enhanced standardization, integration of occupational health metrics, and improved air quality monitoring to ensure reliable exposure assessment and to safeguard both environmental and worker health in the livestock sector. Full article

The present study evaluated the impact of precision feeding using Electronic Sow Feeders (ESFs) during lactation on the nutritional status of Iberian sows and the performance of their offspring under intensive farming conditions. Fifty-three second-parity Iberian sows were assigned to three feeding systems: ESFs (PF), feeding ball (FB), and conventional feeders (CON). Results showed that PF sows had a significantly lower feed disappearance, without affecting body weight and condition, when compared to FB and CON groups (p < 0.05). Although milk yield did not differ significantly among groups, PF piglets exhibited higher weaning weights and average daily weight gains (ADWG), particularly during the second half of lactation (p < 0.05 and p < 0.001, respectively). Metabolic profiling revealed an improved glucose and lipid homeostasis in PF sows, with lower Non-Esterified Fatty Acids (NEFAs) levels and more favorable cholesterol profiles (p < 0.05 for all). No remarkable differences were observed in welfare biomarkers (salivary cortisol and alpha-amylase) among treatments. These findings suggest that precision feeding enhances feed efficiency, stabilizes maternal metabolism, and improves piglet growth in traditional swine breeds (“fatty pigs”) like the Iberian pig, offering a sustainable strategy for intensifying production without compromising animal welfare. Full article

African swine fever (ASF) is a transboundary viral disease that has heavily impacted Southeast Asia since its introduction in 2019. Smallholder pig production systems in Cambodia and the Lao People’s Democratic Republic (the Lao PDR), characterized by low biosecurity, free-ranging practices, and limited veterinary oversight, remain particularly vulnerable. To assess farmers’ awareness and practices regarding ASF, a knowledge, attitudes, and practices (KAP) survey was implemented between March and September 2023 by Agronomes et Vétérinaires Sans Frontières within the framework of the Biosecurity in Pig Farming (BIG) project. A total of 471 pig farmers, including 56% women, were interviewed across eight provinces using a standardized questionnaire (188 in Cambodia and 283 in the Lao PDR). Results showed that ASF awareness was generally high (92% in Cambodia, 66% in the Lao PDR), yet 15% of Cambodian and 30% of Lao respondents expressed doubts about the presence of ASF in their country. While recognition of ASF symptoms was moderate and positively correlated with farmers’ perceived capacity to identify the disease, knowledge of transmission pathways was low and often misaligned with perceptions. Airborne transmission was frequently cited as a risk, and the risks related to visitors and fomites were underestimated by more than 50% of the farmers. Implementation of biosecurity measures (BSM) was limited, with mean scores of 43% in Cambodia and 27% in the Lao PDR. Risky practices such as swill feeding, free-ranging, sharing of boars, traders, and inadequate carcass disposal remained widespread. Statistical analysis identified education level, herd size, knowledge, perceived risks, and perceived benefits of BSM as the main determinants of biosecurity implementation. Farmers with larger herds or stronger commercial orientation demonstrated higher biosecurity adoption, while misconceptions and knowledge gaps remained frequent among smallholder farmers. Strengthening awareness, promoting low-cost and feasible biosecurity practices, and integrating farmer-centred approaches are essential for reducing ASF transmission risks and improving the resilience of smallholder pig production systems in the region. Full article

Background/Objectives: Lawsonia intracellularis (L. intracellularis) is an important intestinal pathogen that causes porcine proliferative enteropathy (PPE) in swine production worldwide. Currently, only a few commercially available vaccines are available for PPE prevention. Methods: In this study, an attenuated L. intracellularis variant of JS-G90 was obtained through subculturing of L. intracellularis JS isolates in McCoy cells for 90 generations, and its immune response was evaluated in pigs. Results: The results demonstrated that pigs who underwent intragastric administration of JS-G90 had lower fecal bacterial shedding and no histopathological lesions, indicating that it was safe in pigs. Therefore, JS-G90 was selected to develop the attenuated PPE vaccine. The immune response of JS-G90 in pigs was further evaluated based on fecal bacterial shedding, histopathological lesions, and humoral and cell-mediated immune responses following challenge with pathogenic L. intracellularis. The results revealed that JS-G90 significantly decreased the copies of L. intracellularis in rectal swabs containing feces and ileum infection (p < 0.001), reduced histopathological lesions in the ileum, and elicited non-specific humoral (IgG and sIgA) and cell-mediated immune responses (p < 0.001) compared with the challenge control and mock groups. Conclusions: In conclusion, the attenuated vaccine JS-G90 is safe and induced humoral and cell-mediated immune responses in pigs against pathogenic L. intracellularis infection. It may serve as an effective strategy for preventing and controlling PPE. Full article

Transmissible gastroenteritis virus (TGEV) represents a critical intestinal pathogen responsible for acute enteritis in pigs, posing significant challenges to global swine production biosecurity. N⁶-methyladenosine (m⁶A), the most abundant epitranscriptomic mark in eukaryotic messenger RNA, has emerged as a regulatory factor in host–virus interactions. Despite its recognized importance, the functional significance of m⁶A modifications during TGEV infection of porcine jejunal epithelial (IPEC-J2) cells remains unexplored. Here, we established a TGEV-infected IPEC-J2 cell model and we employed methylated RNA immunoprecipitation sequencing (MeRIP-seq) to comprehensively profile the m⁶A epitranscriptomic landscape and identify N⁶-methyladenosine-bearing transcripts in IPEC-J2 cells following TGEV challenge. A total of 14,813 m⁶A peaks were identified in the IPEC-J2, distributed in 7728 genes, mainly enriched in the CDS and 3′-UTRs. After TGEV infection, we identified 832 m⁶A peaks and 1660 genes with significant changes. Integrative analysis revealed a direct positive relationship between N⁶-methyladenosine modification abundance and transcript expression levels. Through integrated examination of MeRIP-Seq and RNA-Seq datasets, we identified 105 transcripts bearing m⁶A modifications, which were mainly enriched in the mTOR signaling pathway. Protein–protein interaction (PPI) network and RT-qPCR analysis demonstrated that SOS2 probably acts an important moderator in TGEV infection. This work contributes to understanding the m⁶A modification landscape in the TGEV-swine model and suggests SOS2 as potential target for future antiviral strategies. Full article

Efficient, cost-effective and sustainable pork production remains a primary objective in modern pig farming. However, the extensive use of antibiotics in animal nutrition has raised significant concerns regarding food safety and the emergence of antibiotic-resistant bacteria. These challenges have prompted the search for safe and effective alternatives to antibiotic growth promoters. Sodium butyrate (SB), the sodium salt of butyric acid, has gained considerable attention as a functional feed additive in swine production. Its supplementation has been shown to improve intestinal morphology, regulate gut microbiota composition and enhance immune competence, resulting in better nutrient utilization and growth performance. Moreover, SB supplementation may support environmental sustainability in livestock production by mitigating the emission of harmful gases in swine housing facilities. Although current evidence is limited, in vitro studies have reported promising reductions in NH₃, H₂S and total gas production by 17.96%, 12.26% and 30.30%, respectively. Comparable effects have also been observed in laying hens, where NH₃ emissions were reduced by 26.22%. This review summarizes current knowledge on the application of SB in pig nutrition, focusing on its mechanisms of action, effects on health and productivity, and potential environmental benefits. The findings indicate that SB represents a promising and safe alternative to antibiotics, supporting both animal welfare and sustainable pork production within modern livestock systems. Full article

Streptococcus dysgalactiae subsp. equisimilis (SDSE) has historically been recognized as a human pathogen, yet β-hemolytic streptococci consistent with SDSE have been documented in pigs for nearly a century. To investigate the population structure of porcine SDSE and the phylogenetic relationships between swine and human strains, we characterized 41 isolates recovered from diseased pigs in Quebec, Canada (2019–2022). Infected animals spanned all major production stages and frequently presented with invasive disease, including arthritis, endocarditis, and sudden death. Core-genome phylogenetics resolved two heterogeneous porcine clades separated by long internal branches and clearly distinct from dominant human SDSE lineages. Most porcine isolates were emm-negative or contained structurally altered emm regions compared with human strains. Analysis of Lancefield antigen loci identified a predominant group C lineage and a minority group L lineage, recapitulating historical serogroup distributions described since the early-20th century. Phenotypic testing showed susceptibility to β-lactams and florfenicol but high levels of resistance to tetracycline, macrolides and lincosamides. Detected antimicrobial resistance (AMR) genes correlated well with phenotypes, and multidrug resistance was frequent. Hybrid genome assemblies revealed integrative and mobilizable elements carrying AMR determinants. Collectively, our data indicate that porcine SDSE represents a long-standing, genetically structured, host-adapted population with notable AMR potential, underscoring the need for continued swine SDSE genomic surveillance. Full article

Brachyspira hyodysenteriae is the primary cause of swine dysentery, characterized by bloody to mucoid diarrhea due to mucohaemorhagic colitis in pigs. The disease primarily affects pigs during the growth and finishing stage. The control and prevention of B. hyodysenteriae consists of the administration of antimicrobial drugs, in addition to management and adapted feeding strategies. A worldwide re-emergence of the disease has recently been reported with an increasing number of isolates demonstrating decreased susceptibility to several crucially important antimicrobials in the control of swine dysentery. This compromises the possibilities to eradicate B. hyodysenteriae from infected pig farms. A novel non-antibiotic zinc chelate has been reported to demonstrate positive effects on fecal quality and consistency, general clinical signs, average daily weight gain, and B. hyodysenteriae excretion during and after a 6-day oral treatment. The objective of the present study was to evaluate the zinc chelate (IntraDysovinol^® 499 mg/mL; IntraCare) within an eradication schedule with naturally occurring swine dysentery due to B. hyodysenteriae resistant to pleuromutilins under field conditions in Belgium. We evaluated a 14-day treatment schedule combined with alternative management measures (including partial depopulation of post-weaning facilities and improved external and internal biosecurity measures) and thorough cleaning and disinfection (including 2% NaOH) of the buildings and the sows from day 7 of treatment onwards. This alternative approach for B. hyodysenteriae eradication was evaluated on 18 pig farms over a 5-year period. All enrolled eradication programs were evaluated as successful at least 6–9 months after the finalization of the protocol. In conclusion, the zinc chelate product has an excellent potential for application within an eradication protocol of B. hyodysenteriae that are diagnosed as resistant to pleuromutilins. The combined approach of zinc chelate treatment and alternative management measures is demonstrated to be successful in the eradication of farrow-to-wean, farrow-to-finish, and finishing pig farms under field conditions in Belgium. Full article

The gut-brain axis is a complex communication network linking the gastrointestinal tract and the central nervous system, in which the gut microbiota plays a pivotal role in regulating intestinal homeostasis, immune responses and neuroendocrine functions. This review summarizes current knowledge on the role of the porcine gut microbiota in the functioning of the gut-brain axis and examines nutritional strategies aimed at its modulation. Key production-related stressors, such as weaning, transport and handling, disrupt microbiota composition, increase intestinal permeability and activate the hypothalamic-pituitary-adrenal (HPA) axis, leading to heightened stress responses, impaired immunity and behavioral disturbances. Evidence indicates that supplementation with probiotics, prebiotics, or postbiotics stabilizes the gut microbiota, enhances the production of bioactive metabolites, supports intestinal barrier integrity and alleviates oxidative stress. Such interventions improve adaptation to environmental stress, animal welfare and performance, while potentially reducing the need for antibiotics. Maintaining a balanced gut microbiota is therefore essential for the proper functioning of the neuroendocrine and immune systems in pigs. An integrated approach utilizing omics technologies (metagenomics, metabolomics, proteomics) may further elucidate microbiota-brain interactions and support the development of sustainable and ethical swine production strategies. Full article

Romania faces a double challenge in the swine production sector. On one hand, the European Union’s environmental agenda demands that member states drastically reduce both the carbon footprint and the use of antibiotics in animal husbandry by 2030. On the other hand, the Romanian swine industry still grapples with long-standing internal issues such as excessive fragmentation, a strong dependence on imported piglets and feed materials, and a clear shortage of modern management experience. This study set out to explore how the ADKAR model can serve as a structured approach to help commercial swine farms in Romania transition toward sustainability. To gather relevant data, researchers distributed a five-point Likert-scale questionnaire to 83 farm managers, out of the 361 officially registered commercial swine farms. The instrument was designed to assess how each farm positioned itself across the five ADKAR dimensions. The results revealed that most Romanian farm managers are highly aware of the need for change and show a generally positive attitude toward adopting sustainable practices. However, there remain considerable knowledge gaps and practical limitations, which continue to act as major barriers to effective implementation. The composite ADKAR-S Index, which measures the “sustainability maturity” of each farm, displayed a strong positive correlation with economic performance, particularly the profit margin (r ≈ 0.45, p < 0.001), and a significant negative correlation with antimicrobial use (r ≈ −0.50, p < 0.001). Simply put, farms that are better prepared for organizational transformation tend to perform better financially while also reducing their environmental footprint. The findings suggest that policy efforts should prioritize human capital development, especially through training programs and reinforcement systems such as continuous monitoring and staff incentives, to ensure that sustainable practices are not only adopted but also maintained in the long run. Full article