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Assisted Reproductive Technologies in Production Animals

A Special Issue of Animals (ISSN 2076-2615) belonging to the section "Animal Reproduction".

Deadline for manuscript submissions: closed (31 December 2025) | Viewed by 3898

Editors


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Guest Editor
School of Agriculture and Food Sustainability, The University of Queensland, Gatton, QLD 4343, Australia
Interests: sperm; oocyte; cattle; activation; embryo; cryopreservation; equine; donkey; in vitro fertilization
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
School of Agriculture, Universidad de Buenos Aires, Av San Martin 4453, Buenos Aires 1416, Argentina
Interests: embryos; in vitro fertilization; gene-edition; pig; cattle; cloning

Special Issue Information

Dear Colleagues,

Advancements in assisted reproductive technologies (ARTs) are transforming modern livestock breeding by bridging traditional animal husbandry practices with cutting-edge scientific innovations. Techniques such as in vitro fertilization (IVF), embryo transfer, intracytoplasmic sperm injection (ICSI), and genomic selection enable precise genetic improvements, enhancing productivity, disease resistance, and animal welfare. Moreover, these tools play an increasingly important role in conserving rare and endangered livestock breeds, thereby preserving genetic diversity crucial for sustainable agriculture. By integrating ART with emerging technologies, such as artificial intelligence-driven data analytics, CRISPR-based gene editing, and stem cell-based bioengineering, producers could more effectively meet the growing global demand for high-quality animal products while minimizing environmental impact. This Special Issue invites original research articles, reviews, and case studies focusing on the practical applications, current challenges, and future directions of ART in production animals. Through this collaborative platform, we aim to advance knowledge, foster industry adoption of emerging new practices and contribute to optimizing animal productivity and well-being.

Dr. Andrés Gambini
Dr. Rafael Fernández-Martín
Guest Editors

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Keywords

  • assisted reproductive technologies
  • livestock breeding
  • in vitro fertilization
  • embryo transfer
  • intracytoplasmic sperm injection
  • genetic improvement
  • sustainable agriculture
  • animal welfare
  • cloning
  • in vitro breeding
  • stem cells

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Published Papers (2 papers)

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Research

13 pages, 739 KB  
Article
Factors Influencing the Production Efficiency of Cloned Pigs: A Large-Scale Retrospective Analysis
by Huaxing Zhao, Shouquan Zhang, Xiaopeng Tang, Rong Zhou, Ranbiao Mai, Lvhua Luo, Qiaoyun Su, Sixiu Huang, Zhenfang Wu, Zicong Li, Gengyuan Cai and Junsong Shi
Animals 2026, 16(2), 168; https://doi.org/10.3390/ani16020168 - 7 Jan 2026
Viewed by 1622
Abstract
Pig somatic cell nuclear transfer (SCNT) has valuable applications in agriculture, biomedicine, and life sciences, yet low cloning efficiency remains a major constraint limiting its application. To systematically investigate factors related to the production efficiency of pig cloning, this study conducted a retrospective [...] Read more.
Pig somatic cell nuclear transfer (SCNT) has valuable applications in agriculture, biomedicine, and life sciences, yet low cloning efficiency remains a major constraint limiting its application. To systematically investigate factors related to the production efficiency of pig cloning, this study conducted a retrospective analysis of 367,701 SCNT embryos transferred into 2019 surrogate sows over five years, focusing on breeds of donor cells, the season of embryo transfers, and the number of embryos transferred per surrogate. Our data demonstrate that the genetic background of donor cells is a critical determinant. SCNT embryos generated by wild-type (WT) Pietrain and Duroc pigs yielded significantly higher cloning efficiencies compared to those from Large White and Yorkshire pigs. This breed-specific influence was also observed with genetically modified (GM) donor cells. Nevertheless, within the GM groups, GM-Duroc and GM-Yorkshire showed superior efficiency compared to GM-Large White and GM-Bama. Furthermore, Summer was identified as the least favorable season for embryo transfer, with significantly lower pregnancy rates, delivery rates, and cloning efficiency compared to the other seasons. Importantly, we established that transferring 100–150 embryos per recipient optimized cloning efficiency, significantly outperforming groups receiving higher embryo numbers without compromising pregnancy rates, delivery rates, or average litter sizes. Our findings provide valuable guidance for optimizing large-scale SCNT protocols in swine. Full article
(This article belongs to the Special Issue Assisted Reproductive Technologies in Production Animals)
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11 pages, 985 KB  
Article
Cryoinjuries in Cryopreserved Semen of Ichthyoelephas longirostris with Ethylene Glycol
by Jaider Alonso Martínez-Suarez, José Alonso Espinosa-Araujo and Víctor Atencio-García
Animals 2025, 15(16), 2338; https://doi.org/10.3390/ani15162338 - 10 Aug 2025
Cited by 1 | Viewed by 927
Abstract
Ichthyoelephas longirostris, commonly known as pataló, is an endemic species from Colombia, categorized as endangered due to habitat degradation and overfishing. In this context, semen cryopreservation emerges as an effective strategy for its conservation. This study evaluated the damage caused by semen [...] Read more.
Ichthyoelephas longirostris, commonly known as pataló, is an endemic species from Colombia, categorized as endangered due to habitat degradation and overfishing. In this context, semen cryopreservation emerges as an effective strategy for its conservation. This study evaluated the damage caused by semen cryopreservation using a cryosolution composed of ethylene glycol (EG) at three inclusion levels (6%, 8%, and 10% v/v), skimmed milk powder (3% w/v), and glucose (6% w/v), all prepared in distilled water. A randomized experimental design was employed, with three treatments and a control (fresh semen), using pooled semen from different males (n = 12). Each sample was analyzed in triplicate. Sperm parameters were evaluated through computer-assisted analysis (CASA), and damage to the plasma membrane, mitochondria, and DNA was assessed via flow cytometry. Fresh semen showed low levels of membrane damage (9.6 ± 6.9%), mitochondrial damage (29.1 ± 16.8%), and DNA fragmentation (0.24 ± 0.13%). In thawed semen, the highest membrane damage was observed with 10% EG (79.3 ± 13.0%), while the highest DNA fragmentation occurred with 6% EG (22.5 ± 21.9%). The cryosolution composed of 8% EG showed the best results, with lower levels of damage in the evaluated structures. These findings suggest that the combination of 8% EG, 3% skimmed milk powder, and 6% glucose is an effective formulation to reduce cellular damage during I. longirostris semen cryopreservation, representing a useful tool for conservation programs of this species. Full article
(This article belongs to the Special Issue Assisted Reproductive Technologies in Production Animals)
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