Search Results (819)

This study investigated the effects of varying levels and combinations of calcium (Ca) and magnesium (Mg) supplementation on the diversity, composition, and species differentiation of the rhizosphere soil bacterial community in rainfed maize, aiming to reveal their regulatory mechanisms on the rhizosphere micro-ecosystem. A field micro-plot experiment was conducted with seven treatments: low Ca (U), high Ca (V), low Mg (W), high Mg (X), low Ca and low Mg (Y), high Ca and high Mg (Z), and a control (K, no supplementation). The bacterial communities were analyzed using high-throughput sequencing of the 16S rRNA gene, and the data were processed using the QIIME2 pipeline, as well as multivariate statistical analyses, and LEfSe. The main results demonstrated that Ca and Mg supplementation significantly altered the rhizosphere bacterial community structure (beta-diversity). Analysis of Similarities (ANOSIM) indicated significant differences between treatments (R > 0.4, p < 0.01). LEfSe analysis successfully identified key biomarkers responsive to different treatments. For instance, high Ca treatment significantly enriched the genus Clostridium within the phylum Firmicutes, whereas high Mg treatment specifically enriched the genus Lysobacter. Furthermore, Ca-Mg interactions exhibited non-additive effects, and the coupled Ca-Mg supplementation treatments (Y, Z) formed unique species assemblages. As key environmental drivers, Ca and Mg supplementation specifically reshaped the rhizosphere bacterial community through “environmental filtering” in rainfed maize. This study provides a theoretical basis for understanding the microbiological pathways through which secondary element fertilizers influence soil health, offering practical implications for precisely managing rhizosphere micro-ecology through Ca and Mg supplementation to promote the sustainable development of dryland farming. Full article

Neonicotinoid insecticides were initially hailed as safer alternatives to organochlorine and organophosphate pesticides due to their perceived lower toxicity to non-target organisms. However, it has been recently discovered that sublethal exposure to neonicotinoids negatively affects beneficial arthropods that are essential for a functional ecosystem. These beneficial arthropods include pollinators, biological control agents, and decomposers. This review synthesizes current research on the physiological, behavioral, and reproductive consequences of neonicotinoids on non-target arthropods and their broader ecological impact. The chemical and physical properties of neonicotinoids raise concerns about long-term ecological consequences of neonicotinoid use because these chemicals are persistent in plants and soil, which contributes to prolonged exposure risks for organisms. Sublethal doses of neonicotinoids can disrupt the ecological services provided by these organisms by impairing essential biological processes including motor function, odor detection, development, and reproduction in insects, while also altering behavior such as foraging, mating, and nesting. Furthermore, neonicotinoid exposure can alter community structure, disrupting trophic interactions and food web stability. Recognizing the sublethal impacts of neonicotinoids is critical for the development of more sustainable pest management strategies. It is imperative that future research investigates the underlying mechanisms of sublethal toxicity and identifies safer, more effective approaches to neonicotinoid-based pest control to mitigate adverse ecological effects. Incorporating this knowledge into future environmental risk assessments will be essential for protecting biodiversity and maintaining ecosystem functionality. Full article

To investigate the performance of horizontally reinforced composite foundations in resisting surface rupture of normal faults, this study designed and conducted a series of physical model tests. A systematic comparative analysis was performed on the fracture resistance of sites with three-layer sand, five-layer sand, and three-layer clay geogrid horizontally reinforced composite foundations under 70° normal fault dislocation. The results indicate that significant changes in earth pressure serve as a precursor indicator of fault rupture, and their evolution process reveals the internal energy accumulation and release mechanism. Increasing the number of geogrid layers significantly enhances the lateral confinement of the foundation, resulting in a narrower macro-rupture zone located farther from the structure in sand sites, and promotes the formation of a step-fault scarp deformation mode at the surface, which is more conducive to structural safety. Under identical reinforcement conditions, the clay site exhibited comprehensively superior fracture resistance compared to the sand site due to the soil cohesion and stronger interfacial interaction with the geogrids, manifested as more significant deviation of the rupture path, and lower microseismic accelerations and structural strains transmitted to the building. Comprehensive analysis confirms that employing geogrid-reinforced composite foundations can effectively guide the surface rupture path and improve the deformation pattern, representing an effective engineering measure for mitigating disaster risk for buildings spanning active faults. Full article

The limited availability of competent foundation soils in rapidly urbanizing regions makes construction on weak clayey deposits increasingly unavoidable. Such soils typically exhibit low shear strength, high compressibility, and pronounced deformation under undrained conditions, posing significant risks to structural safety and long-term serviceability. In this study, the effect of basalt fiber inclusion on the undrained shear behavior of clay soil obtained from the Kızılyer region of Eskişehir, Türkiye, was experimentally investigated using a ring shear apparatus. Initially, soil classification and index property tests were performed to characterize the material. Subsequently, clay specimens were reinforced with varying basalt fiber contents and subjected to large-strain shearing conditions. The evolution of peak and residual shear strength with increasing fiber dosage was systematically evaluated. The results indicate that basalt fiber reinforcement leads to a substantial enhancement in both peak and residual shear strength and contributes to improved post-peak ductility. The observed improvements are primarily attributed to fiber–soil interaction mechanisms, including tensile bridging and crack-arrest effects, which modify the failure process and delay shear localization. Overall, the findings demonstrate that basalt fiber represents an environmentally compatible and mechanically effective alternative for sustainable soil improvement applications, particularly in clayey soils subjected to undrained loading conditions. Full article

The dynamic characterization of historical buildings located in a complex geological and seismological context is essential to assess seismic vulnerability and to guide conservation strategies. This study presents a non-invasive, ambient vibration-based, investigation of the Norman Castle of Aci Castello (Sicily, Italy), applying Horizontal to Vertical Spectral Ratio (HVSR), Horizontal to Horizontal Spectral Ratio (HHSR), and Random Decrement Method (RDM) to evaluate the structure’s dynamic behavior and potential Soil–Structure Interaction (SSI) effects. The fundamental site frequency, estimated within a broad plateau in the range 2.05–2.70 Hz, does not overlap with the structural frequencies of the castle, which range approximately from 6.30 Hz to 9.00 Hz in the N–S structural direction and from 3.50 Hz to 8.50 Hz in the E–W direction, indicating absence of global SSI resonance. However, the structure exhibits a complex multimodal response, with direction-dependent behavior evident both in spectral peaks and in damping ratios, ranging from 2.10–7.73% along N–S and 0.90–5.84% along E–W. These behaviors can be interpreted as possibly linked to structural complexity and the interaction with the fractured volcanic substrate, characterized by shallow cavities, as well as to the material degradation of the masonry. In particular, the localized presence of subsurface voids may induce a perturbation of the low-frequency ambient vibration wavefield (e.g., microseisms), producing a localized increase in spectral amplitude observed at Level I. The analysis indicates the absence of global SSI resonance due to the lack of overlap between site and structural fundamental frequencies, while significant local SSI effects, mainly related to cavity-induced wavefield perturbation, are observed and may represent a potential vulnerability factor. These findings highlight the relevance of vibration-based diagnostics for heritage vulnerability assessment and conservation strategies. Full article

This study quantifies vertical earth pressure on the roofs of box culverts under high fills in valley terrain using centrifuge model tests with expanded polystyrene (EPS) geofoam for load mitigation. We compare buried-type culverts with valley-terrain high-fill culverts and isolate the effects of the EPS installation height and panel thickness on the roof pressure and the associated concentration factor. The analysis of fill settlement elucidates the terrain-dependent load reduction mechanism and the efficacy of EPS panels. The results show that the roof pressure increases with EPS installation height but decreases and then plateaus once the panel thickness exceeds 75 cm; the load reduction benefit weakens when the installation height exceeds 2 m. Optimal performance is achieved with panels installed at 2 m and with a 75 cm thickness, which lowers applied loads while maintaining structural stability. These findings clarify soil–structure interactions in complex topography and provide practical guidance for deploying EPS in high-fill valley projects. Full article

Laterally loaded slender piles present a classic soil–structure interaction problem where pile displacements and flexural demands are governed by the mobilized lateral resistance of the surrounding soil and the axial-bending capacity of the reinforced concrete section. In response to increasing pressure to reduce embodied emissions, this study develops LAVERCO, an optimization framework for cost- and CO₂-efficient design of bored reinforced-concrete piles in cohesive soils subjected to combined lateral and axial actions. The framework integrates Eurocode-based geotechnical checks with full N–M section verification of the RC pile and applies a genetic algorithm over a multi-parametric grid of lateral load, vertical load, and undrained shear strength, using economic cost and embodied CO₂ as alternative single objectives. Rank-based (Spearman) sensitivity analysis quantifies how actions, soil strength, and design variables influence the optimal solutions. The results reveal two consistent geometry regimes: CO₂-optimal piles are systematically longer and slimmer, while COST-optimal piles are shorter and thicker. In both cases, the objective is dominated by pile length and is reduced by higher undrained shear strength; vertical load has a moderate direct effect, while horizontal load contributes mainly through deflection and bending checks. Feasibility improves significantly in stronger clays, and CO₂-optimal geometries generally incur higher costs, clarifying the trade-off between economic and environmental performance. The framework provides explicit geometry-level guidance for selecting bored pile designs that balance cost and embodied CO₂ across a wide range of soil and loading conditions and can be directly applied in both preliminary and detailed designs. Full article

Fast-running flies (Diptera: Hybotidae) play an important role as predators in agricultural landscapes. This semi-field study examined the effects of pesticides on Hybotidae communities and their role in natural pest control in three winter wheat management systems (organic, conventional, and hybrid (no chemical synthetic pesticides with optimized use of nitrogen fertilizers)) in Brandenburg, Germany. To evaluate the impact of management practices, sweep netting and eclector trapping were carried out over three years (2020–2022) at the plot scale. Hybotidae abundance fluctuated across the management systems and collection methods, with no consistent trend linked to pesticide use. However, an increase in the abundance of Hybotidae in 2022, especially in eclector trapping, indicated that year-to-year fluctuations were pronounced and likely driven by environmental factors, such as climate and soil moisture, rather than management practices. The community structure showed a high degree of similarity among all management systems, but species diversity displayed pronounced interannual variation, suggesting complex ecological interactions. Sweep netting collections indicated positive predator–prey associations in every management system, pointing to a generally stable trophic structure. The study also demonstrates that using multiple insect collection methods is crucial for accurately assessing insect diversity and abundance. Further research is needed to fully understand species diversity, predator–prey dynamics, and their implications for sustainable agriculture. Full article

According to climate projections, the Pannonian region is expected to experience an increasing frequency of drought events. This trend affects not only agricultural areas but also natural grasslands. The Festuca wagneri species, selected for this study, is a dominant and well-adapted grass in dry natural habitats. A total of 54 Festuca wagneri individuals were examined across three soil types: sand, loam, and clay. In each soil type, 18 plants were assessed for drought tolerance. Water was applied at three dosage levels: 200, 300, and 400 mL. The experiment was conducted between 4 April and 18 July 2024, during which the total weight of the pots and the amount of drained water were measured regularly. All data processing and statistical analyses were performed in R version 4.3.2. A three-way factorial ANOVA was used to evaluate main and interaction effects. Model residuals were tested for normality (Shapiro–Wilk test) and homoscedasticity using diagnostic plots. The results showed that Festuca wagneri individuals tolerated even the lowest soil moisture levels induced by low water-holding capacity of the soil and low water input. This indicates that the species can be effectively used in grassland management and restoration under future climate change scenarios. The main differences were observed among soil types, highlighting the crucial importance of soil structure when establishing this species. Loam soils, already near optimal, respond best to moderate. Full article

The rhizosheath plays a critical but poorly understood role in plant–microbe interactions. However, it still remains unclear how host selection versus geographical isolation contributes to microbial community assembly within the rhizosheath. This study characterized the bacterial and fungal communities in the rhizosheath and surrounding bulk soil of Leymus racemosus using 16S rRNA and ITS high-throughput sequencing. Results showed that the bacterial community was strongly shaped by host selection within the rhizosheath, based on significantly reduced α-diversity and distinct β-diversity (Permutation tests, p < 0.001) compared to bulk soil. Furthermore, the core bacterial community structure was highly similar between the two geographically separated sites (PERMANOVA, p = 0.089). In contrast, the fungal community exhibited weaker habitat specificity but showed significant, though weak, geographical divergence (β-diversity, Permutation tests, p = 0.028). The explanatory power of geographical distance for fungal community variation was low (R² = 0.095) and less than that of the rhizosheath microhabitat (R² = 0.142). In conclusion, the rhizosheath imposes a strong filtering effect on bacterial communities. The weaker habitat specificity and stronger geographical signal observed for fungi suggest potential regulation by local dispersal limitation or historical colonization processes. This study provides insights into the assembly mechanisms of the plant rhizosphere microbial community. Full article

Cercophora species, typically known as saprobes or coprophiles, have occasionally been isolated from healthy roots and have recently been recognized as endophytes. Their dark-pigmented structures suggest adaptation traits similar to dark septate endophytes, although their symbiotic potential remains unclear. This study isolated and characterized Cercophora sp. NPKC241 from mung bean roots grown under artificial drought in soils with different fertilization histories, using PCR-based DNA sequencing and morphological observation. Its effects on legume growth were subsequently evaluated through pot inoculation experiments under drought. These experiments focused on mung bean, a species known to exhibit significant reductions in chlorophyll content and yield under drought conditions. Among 29 isolates, Cercophora sp. consistently promoted legume growth. In mung bean, it increased shoot and root mass, chlorophyll content, and root elongation under both optimal and water-limited conditions. Under drought, inoculated plants showed approximately threefold higher chlorophyll levels, two- to threefold greater biomass, and roots approximately 5 cm longer than the control, indicating mitigation of drought-induced physiological decline. These findings suggest that Cercophora sp. can act as a beneficial root-associated fungus, enhancing legume performance under drought. Future studies will further explore this interaction by underlying physiological mechanisms and the field-level application potential. Full article

Partially frozen soil (PFS) is comprises of coexisting unfrozen water and ice within its pores at subzero temperatures. The review paper examines how unfrozen water content (UWC) and pore ice content interact during phase changes under near-freezing conditions, governed by microscopic thermodynamic equilibrium. Key theories describing why UWC persists (premelting, disjoining pressure) and the soil freezing characteristic curve (SFCC), along with measurement techniques, including the gravimetric approach to advanced nuclear magnetic resonance for characterization of water content. The influence of the water–ice phase composition on mechanical behavior is discussed, signifying pore pressure and effective stress. Various modelling approaches categorized into empirical SFCC, physio-empirical estimations, and emerging machine learning and molecular simulations are evaluated for capturing predictions in PFS behavior. The relevance of PFS to infrastructure foundation, tailings dams, permafrost slope stability, and climate change impacts on cold regions’ environmental geotechnics is also highlighted as a challenges in practical application. Hence, understanding pore pressure dynamics and effective stress in PFS is critical when assessing frost heave, thaw weakening, and the overall performance of geotechnical structures in cold regions. By combining micro-scale phase interaction mechanisms and macro-scale engineering observations, this review paper provides a theoretical understanding of the underlying concepts vital for future research and practical engineering in cold regions. Full article

Vegetation heterogeneity supports biodiversity, while homogeneity limits it. In the Great Plains, fire and herbivory enhance ecosystem function by increasing spatial heterogeneity. However, quantifying their effects on plant functional traits and spectral diversity remains challenging due to landscape complexity and scaling limitations. Hyperspectral remote sensing offers a high-resolution approach to assessing these dynamics, improving the evaluations of post-fire recovery and vegetation function. This study examines the impact of fire on plant functional traits and spectral diversity within a savanna landscape in the Edwards Plateau, Texas, using airborne hyperspectral and multispectral imagery. Specifically, it aims to (1) quantify the spatial patterns of plant functional traits and spectral diversity, (2) assess fire’s effects on these patterns, and (3) evaluate how soil type, woody structure, and burn patterns mediate fire responses. High-resolution airborne images from 2018 (pre-fire) and 2020 (post-fire) were analyzed to classify burned and unburned areas, pre-fire woody cover, and derive spectral indices representing plant functional traits, β-diversity components, and spectral evenness. The results indicate that temporal patterns in spectral diversity were driven primarily by soil properties and weather, with limited evidence that fire altered spectral evenness or β-diversity across soils. In contrast, spectral indices showed clearer—but still soil-dependent—fire effects: declines in canopy structure, greenness, and chlorophyll content were less pronounced in burned areas, indicating that fire partially moderated late-season senescence. Fire had a substantial influence on spatial patterns of spectral evenness (but not β-diversity) and vegetation spectral functional traits, and fire and dry-down increased spatial heterogeneity in spectral evenness and in spectral indices indicative of biophysical and biochemical traits across scales. These findings demonstrate that environmental drivers, particularly soil–moisture interactions and interannual moisture variability, exert a stronger control over post-fire spectral diversity than fire alone. Hyperspectral imaging effectively captured these dynamics, supporting its role in monitoring post-fire vegetation responses. In addition to the use of hyperspectral imaging, fire management strategies should consider broader ecological drivers, including soil and weather interactions, to improve the assessments of ecosystem resilience and recovery. Full article

Accurate seismic safety assessment of nuclear power plant (NPP) structures with pile foundations on soft soil sites requires consideration of soil nonlinearity and pile–soil–structure interaction (PSSI). This study develops an efficient partitioned SSI framework, where the nonlinear soil response is simulated using the Davidenkov skeleton curve combined with a modified Masing rule and solved by an explicit time integration scheme, while the structural dynamics are evaluated using the modal superposition method. The framework is applied to a pile-supported CAP1400 NPP model on deep soft soil, with both piles and the superstructure modeled as elastic. Two computational schemes are examined: (a) explicit integration of the soil while treating the piles and structure as an integrated system analyzed via modal superposition; and (b) explicit integration of both soil and piles, with the structure analyzed using modal superposition. Under pulse excitation, both schemes yield comparable dynamic responses, whereas scheme (b) improves computational efficiency by over threefold (88 h vs. 293 h). Results using scheme (b) under RG1.60 excitation show that soil nonlinearity reduces and delays structural responses but increases pile bending moments and stress concentration, demonstrating the framework’s effectiveness and practicality for nonlinear SSI analysis of NPP structures. Full article

The accumulation of microplastics in agricultural soils poses a serious threat to both crop production and ecosystem health. To explore potential remediation strategies, we conducted a two-factor pot experiment (PE-MPs × TSB). This study was designed to systematically analyze the interactive effects of polyethylene microplastics (PE-MPs) and tobacco stalk-derived biochar (TSB) on soil properties, physiological characteristics, and growth indicators of wheat. Results indicated that TSB addition significantly increased soil pH, organic matter, and available potassium content, which was associated with a mitigation of the soil acidification and nutrient imbalance observed under PE-MPs. Physiologically, TSB was linked to higher activities of antioxidant enzymes (SOD and POD) and maintained leaf chlorophyll content and photosynthetic function, thereby consistent with a reduction in oxidative stress and better maintenance of growth in the presence of PE-MPs. Furthermore, partial least squares structural equation modeling (PLS-SEM) supported a hypothetical cascading pathway for TSB’s dominant influence: soil improvement → physiological mitigation → growth recovery. The total effect of TSB on biomass (0.71) was substantially greater than that of PE-MPs (0.01). This study proposes a conceptual model and provides correlative evidence that is consistent with multi-level processes through which TSB may alleviate PE-MPs stress, thereby providing theoretical support for the resource utilization of agricultural waste and the green remediation of microplastic-contaminated soil. Full article