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17 pages, 4203 KB  
Article
Experimental and Numerical Investigation of Vibration-Based Piezoelectric Energy Harvesting Device
by Zhan Zhelev, Lukasz Kloda, Simona Doneva and Emil Manoach
Energies 2026, 19(4), 932; https://doi.org/10.3390/en19040932 - 11 Feb 2026
Viewed by 185
Abstract
A composite beam consisting of two layers is experimentally tested as an energy harvesting device. The substrate layer is made of aluminum and the piezoelectric layer is glued at 90% of the length of the alumina layer. The beam is clamped at one [...] Read more.
A composite beam consisting of two layers is experimentally tested as an energy harvesting device. The substrate layer is made of aluminum and the piezoelectric layer is glued at 90% of the length of the alumina layer. The beam is clamped at one end and is free at the other. The cantilever is subjected to periodic kinematic excitation, and the tip acceleration as well as the generated electricity are measured. A 3D finite element model of the beam is created and the coupled mechanical and electrical fields are studied numerically. The results are compared with those obtained experimentally. A parametric study is conducted to investigate the influence of the loading parameters (frequency and amplitude of excitation) and the electric resistance in the circuit on the generated electricity. Conclusions about the optimal conditions with respect to energy harvesting are made. The importance of proper modelling of the contact between the PZT layer and the substrate is demonstrated. Full article
(This article belongs to the Special Issue Vibration Energy Harvesting)
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23 pages, 6525 KB  
Article
An Analysis of the Vacuum Generation Mechanism and Prototype Study of Negative-Pressure Suction-Type Cuttings Reduction Equipment
by Xin Wang, Bo Zhang, Zhuo Wang and Hongwen Ma
Processes 2026, 14(4), 618; https://doi.org/10.3390/pr14040618 - 10 Feb 2026
Viewed by 168
Abstract
In the context of increasingly complex offshore drilling operations and stricter environmental regulations, the efficient handling and volume reduction of drilling cuttings has emerged as a crucial focus in the advancement of solids control equipment. “Airflow-assisted screening” is a technique that uses directed [...] Read more.
In the context of increasingly complex offshore drilling operations and stricter environmental regulations, the efficient handling and volume reduction of drilling cuttings has emerged as a crucial focus in the advancement of solids control equipment. “Airflow-assisted screening” is a technique that uses directed air currents to enhance the separation of solid cuttings from drilling fluid on a shaker screen, thereby improving dewatering efficiency and reducing waste volume during drilling. This study proposes and designs novel negative-pressure suction-type cuttings reduction equipment by integrating this technology with screw conveying principles. The system features a compact, vacuum-generator-centered design that integrates suction and screening. Key components were optimized, and a monitoring scheme was implemented for real-time performance evaluation. In the mechanism analysis, the relationship between inlet pressure, geometric parameters, and suction performance was explored based on Bernoulli’s principle and Laval nozzle characteristics, and internal flow field characteristics were revealed through computational fluid dynamics (CFDs) simulations. In the experimental section, a prototype system and testing platform were constructed to evaluate the effects of inlet pressure and screen mesh configurations on suction and screening performance. The results indicate that the system achieved optimal performance at an inlet pressure of 400 kPa with a 100-mesh screen, reaching a cuttings reduction efficiency of 9.225%. This study effectively validates the theoretical and simulation findings, providing technical support for the application of this equipment in complex drilling environments and demonstrating strong potential for practical implementation. Full article
(This article belongs to the Section AI-Enabled Process Engineering)
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28 pages, 3642 KB  
Article
In Vitro Phytochemical Profiling, and Antioxidant Activity Analysis of Callus and Cell Suspension Cultures of Washingtonia filifera Elicited with Chitosan
by Huda Enaya Mahood, Virginia Sarropoulou, Thalia Tsapraili and Thiresia-Teresa Tzatzani
Agronomy 2026, 16(1), 106; https://doi.org/10.3390/agronomy16010106 - 31 Dec 2025
Viewed by 527
Abstract
Washingtonia filifera is important for its ecological, economic, cultural, horticultural, ornamental, and medicinal potential. Elicitation of in vitro cultures presents a promising and efficient method for the large-scale production of valuable bioactive compounds. This study assessed the effect of chitosan concentration (0, 20, [...] Read more.
Washingtonia filifera is important for its ecological, economic, cultural, horticultural, ornamental, and medicinal potential. Elicitation of in vitro cultures presents a promising and efficient method for the large-scale production of valuable bioactive compounds. This study assessed the effect of chitosan concentration (0, 20, 40, 60, 80, 100 mg L−1) on biomass growth [fresh weight (FW), dry weight (DW)] and phytochemical profile [total phenolic content (TPC), total flavonoid content (TFC), DPPH antioxidant activity, total phenolic productivity (TPP), total flavonoid productivity (TFP)] in W. filifera callus and cell suspension cultures. Among different plant growth regulator combinations tested, 3 mg L−1 2,4-D + 0.5 mg L−1 2ip gave higher callus induction (90%) (MS medium, 12 weeks). A maximum growth curve (FW: 180 mg) of cell suspension culture was achieved 7 weeks after initiation (shaker at 90 rpm for 24 h). Cell suspension exhibited higher FW, DW, TPC, TFC, DPPH, TPP, and TFP than callus, while flavonoid production was higher than phenolic production. FW and DW were higher in both systems, with 40 mg L−1 chitosan. Chitosan at 60 mg L−1 best enhanced the phytochemical profile of both the 4-week solidified callus and the 7-week liquid cell suspension (TPC: 29.9 and 32.1 mg GAE g−1 DW; TFC: 40.5 and 56.1 mg QE g−1 DW; TPP: 969.2 and 1122.6 mg L−1; TFP: 1313.9 and 1521.7 mg L−1; DPPH: 87.4 and 92.3%), respectively, while 40 mg L−1 chitosan was equally effective regarding DW, TFC, and TFP in cell suspension. Chitosan elicitation provides a powerful strategy to upregulate phenolic and flavonoid biosynthesis in W. filifera in vitro systems, conferring superior antioxidant potential. The identification of peak elicitation parameters (chitosan concentration, exposure time) allows for the targeted enhancement of bioactive compound yields, suggesting a viable path for industrial bioproduction and commercialization in pharmaceuticals, nutraceuticals, and functional foods, leveraging bioreactor technology for efficient scale-up. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Regeneration Techniques for Crop Enhancement)
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28 pages, 60690 KB  
Article
A Modeling Approach for Assessing Vibration Immunity in Hydrogen Fuel Cell Stack for Aeronautical Applications
by Giovanni Fasulo, Simone Gallas, Hervé Denayer, Oskar Ekblad, Giancarlo Kosova and Mattia Barbarino
Appl. Sci. 2026, 16(1), 69; https://doi.org/10.3390/app16010069 - 20 Dec 2025
Viewed by 409
Abstract
Fuel cells offer a promising route to eliminating in-flight emissions from regional aviation, but certification requires proof that stacks can withstand the vibration and shock environment of turboprop aircraft. As part of the EU-funded NEWBORN project, we combined detailed finite element modeling with [...] Read more.
Fuel cells offer a promising route to eliminating in-flight emissions from regional aviation, but certification requires proof that stacks can withstand the vibration and shock environment of turboprop aircraft. As part of the EU-funded NEWBORN project, we combined detailed finite element modeling with shaker tests to evaluate the vibration immunity of PowerCell Group’s prototype stack. The numerical model combined an orthotropic, two-zone 3D mesh of the cell package with reduced-order representations of plates, compression bands, disc springs and the mounting cage. The assembled stack was excited between 10 and 300 Hz using pseudo-random and sine-sweep inputs up to 2.0 g, from which 54 frequency response functions were obtained. The tuned model accurately reproduced the first global modes and captured the overall dynamic behavior with good, though not perfect, agreement. The combined numerical–experimental methodology therefore offers a framework for refining test campaigns and delivering early, qualitative evidence of vibration immunity in fuel cell stacks destined for flight. Full article
(This article belongs to the Special Issue Advances in Aerostructural Analysis, Design, and Optimization)
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13 pages, 3049 KB  
Article
A Hybrid Piezoelectric and Photovoltaic Energy Harvester for Power Line Monitoring
by Giacomo Clementi, Luca Tinti, Luca Castellini, Mario Costanza, Igor Neri, Francesco Cottone and Luca Gammaitoni
Actuators 2026, 15(1), 1; https://doi.org/10.3390/act15010001 - 19 Dec 2025
Viewed by 602
Abstract
Monitoring the health of power lines (PL) is essential for ensuring reliable power delivery, facilitating predictive maintenance, and maintaining a resilient grid infrastructure. Given the extensive length of PL networks, large numbers of wireless sensor nodes must be deployed, often in remote and [...] Read more.
Monitoring the health of power lines (PL) is essential for ensuring reliable power delivery, facilitating predictive maintenance, and maintaining a resilient grid infrastructure. Given the extensive length of PL networks, large numbers of wireless sensor nodes must be deployed, often in remote and harsh environments where battery replacement is costly and impractical. To address these limitations, this work proposes a hybrid energy-harvesting approach that combines piezoelectric and photovoltaic (PV) technologies to enable long-term, battery-free PL monitoring. The primary energy source is a compact, tunable, magnetically coupled piezoelectric vibrational energy harvester (VEH) that exploits local magnetic field distribution, inducing mechanical excitation of a cantilever and enabling the harvesting of vibrational energy near the PL at a frequency of 50 Hz. A complementary PV harvester is integrated to ensure operation during power outages or conditions where the piezoelectric excitation is reduced, thereby enhancing system robustness. Electromechanical characterization and a lumped-parameter model show good agreement with experimental results of the proposed VEH. The system is validated both on a PL test bench (5 A–10 A) and through inertial excitation using an electrodynamic shaker, demonstrating stable performance across a wide range of operating conditions. The combined hybrid architecture highlights a promising pathway toward self-sustaining, maintenance-free sensor nodes for next-generation power line monitoring. Finally, we demonstrate the feasibility of using such system for powering a WSN node by comparing the power produced by the proposed system with the power consumption of a potential application. Full article
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29 pages, 6963 KB  
Article
Low-Cost Angular-Velocity Measurements for Sustainable Dynamic Identification of Pedestrian Footbridges: A Case Study of the Footbridge in Gdynia (Poland)
by Anna Banas
Sustainability 2025, 17(23), 10456; https://doi.org/10.3390/su172310456 - 21 Nov 2025
Viewed by 497
Abstract
This study investigates the practical value of angular-velocity measurements in the dynamic identification of pedestrian footbridges, addressing the need for reliable yet cost-effective diagnostics for slender civil structures. A comprehensive experimental campaign on a steel footbridge in Gdynia combined ambient vibration tests, forced [...] Read more.
This study investigates the practical value of angular-velocity measurements in the dynamic identification of pedestrian footbridges, addressing the need for reliable yet cost-effective diagnostics for slender civil structures. A comprehensive experimental campaign on a steel footbridge in Gdynia combined ambient vibration tests, forced excitation (light and heavy shakers), and controlled pedestrian loading. Synchronous translational accelerations and rotational velocities from MEMS sensors enabled evaluation of both bending and torsional responses. Three identification techniques—Peak Picking (PP), Frequency Domain Decomposition (FDD), and Stochastic Subspace Identification (SSI)—were applied and compared with a validated beam–shell FEM developed in SOFiSTiK. The results show that rotational data improve mode-shape interpretation and classification, notably resolving a coupled torsional–vertical mode (VT2) that was ambiguous in acceleration-only analyses. The fundamental frequency of 3.1 Hz places the bridge in a resonance-prone range; field tests confirmed predominantly vertical response, with horizontal accelerations < 0.05 m/s2 and peak vertical accelerations exceeding comfort class CL3 during synchronised walking of six pedestrians (≈2.55 m/s2) and jumping (up to 3.61 m/s2). Overall, the outcomes highlight that low-cost gyroscopic sensing offers substantial benefits for structural system identification and mode-shape characterization, enriching acceleration-based diagnostics and strengthening the basis for subsequent analyses. By reducing the financial and material demands of vibration testing, the proposed approach contributes to more sustainable assessment and maintenance of pedestrian bridges, aligning with resource-efficient monitoring strategies in civil infrastructure. Full article
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21 pages, 4047 KB  
Article
Natural Frequency and Damping Characterisation of Aerospace Grade Composite Plates
by Rade Vignjevic, Nenad Djordjevic, Javier de Caceres Prieto, Nenad Filipovic, Milos Jovicic and Gordana Jovicic
Vibration 2025, 8(4), 72; https://doi.org/10.3390/vibration8040072 - 13 Nov 2025
Viewed by 750
Abstract
The natural frequencies and damping characterisation of a new aerospace grade composite material were investigated using a modified impulse method combined with the half power bandwidth method, which is applicable to the structures with a low damping. The composite material of interest was [...] Read more.
The natural frequencies and damping characterisation of a new aerospace grade composite material were investigated using a modified impulse method combined with the half power bandwidth method, which is applicable to the structures with a low damping. The composite material of interest was unidirectional carbon fibre reinforced plastic. The tests were carried out with three identical square 4.6 mm thick plates consisting of 24 plies. The composite plates were clamped along one edge in a SignalForce shaker, which applied a sinusoidal signal generated by the signal conditioner exiting the bending modes of the plates. Laser vibrometer measurements were taken at three points on the free end so that different vibrational modes could be obtained: one measurement was taken on the longitudinal symmetry plane with the other two 35 mm on either side of the symmetry plane. The acceleration of the clamp was also recorded and integrated twice to calculate its displacement, which was then subtracted from the free end displacement. Two material orientations were tested, and the first four natural frequencies were obtained in the test. Damping was determined by the half-power bandwidth method. A linear relationship between the loss factors and frequency was observed for the first two modes but not for the other two modes, which may be related to the coupling of the modes of the plate and the shaker. The experiment was also modelled by using the Finite Element Method (FEM) and implicit solver of LS Dyna, where the simulation results for the first two modes were within 15% of the experimental results. The novelty of this paper lies in the presentation of new experimental data for the natural frequencies and damping coefficients of a newly developed composite material intended for the vibration analysis of rotating components. Full article
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20 pages, 10582 KB  
Article
Glutamatergic Neurons in the Cerebellar Lateral Nucleus Contribute to Motor Deficits Induced by Chronic Sleep Disturbance
by Jian Zhu, Wan-Qiao Qi, Ling-Xi Kong, Yan-Mei Lin, Feng-Fei Ding, Zhi-Li Huang and Wei-Min Qu
Brain Sci. 2025, 15(11), 1185; https://doi.org/10.3390/brainsci15111185 - 31 Oct 2025
Viewed by 970
Abstract
Background/Objectives: The cerebellum is essential for motor coordination and has recently been implicated in sleep-related disorders. However, the neural mechanisms linking sleep disruption to motor dysfunction remain poorly understood. This study aimed to elucidate the roles of the deep cerebellar nuclei (DCN), [...] Read more.
Background/Objectives: The cerebellum is essential for motor coordination and has recently been implicated in sleep-related disorders. However, the neural mechanisms linking sleep disruption to motor dysfunction remain poorly understood. This study aimed to elucidate the roles of the deep cerebellar nuclei (DCN), particularly the lateral nucleus, in motor dysfunction induced by chronic sleep disruption (CSD). Methods: Using a validated mouse model of CSD with periodic sleep fragmentation induced by an orbital shaker during the light phase, we assessed neuronal activation via c-Fos immunostaining and performed chemogenetic manipulation of glutamatergic neurons within the lateral nucleus. Behavioral performance was evaluated using open-field and rotarod tests. Results: CSD selectively increased c-Fos expression in the lateral nucleus, with no significant changes observed in other DCN subregions. Chemogenetic activation or ablation of glutamatergic neurons in the lateral nucleus decreased locomotor activity in the open-field test and shortened latency to fall in the rotarod task. Conversely, chemogenetic inhibition of these neurons attenuated CSD-induced impairments, restoring locomotor performance toward control levels. Conclusions: Our findings provide direct experimental evidence that glutamatergic neurons in the lateral nucleus play a crucial role in mediating CSD-induced motor dysfunction. These results highlight the cerebellar contribution to the interplay between sleep and motor control and identify a potential target for therapeutic intervention in sleep-related motor disorders. Full article
(This article belongs to the Section Sensory and Motor Neuroscience)
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20 pages, 2608 KB  
Article
Influence of Vibration on Servo Valve Performance and Vibration Suppression in Electro-Hydraulic Shaking Table
by Tao Wang, Sizhuo Liu, Zhenyu Guo and Yuelei Lu
Machines 2025, 13(10), 913; https://doi.org/10.3390/machines13100913 - 3 Oct 2025
Viewed by 783
Abstract
With the rapid progress of industrial technology in recent years, servo controllers have the characteristics of precise control and short response time and are widely used in different industrial fields. As for the electro-hydraulic servo valve being an important control element of the [...] Read more.
With the rapid progress of industrial technology in recent years, servo controllers have the characteristics of precise control and short response time and are widely used in different industrial fields. As for the electro-hydraulic servo valve being an important control element of the entire hydraulic system, the quality of its own characteristics has a significant impact on the normal operation and safety of the mechanical equipment. Therefore, the working stability of the servo valve in actual operation is of great importance to its body and the overall servo system. Similarly, during the vibration test of the electro-hydraulic servo shaking table, servo valve inevitably experiences various vibrations and shocks, which requires the servo system to be able to withstand the test and assessment under the extreme conditions in actual operation to ensure the smooth operation. This paper takes function of the shaker as the research target and studies the servo valve under various vibration conditions by constructing a digital modeling system. On this basis, an adaptive format filter is established, and corresponding vibration suppression methods are adopted for the vibration conditions inside the system. Finally, simulation examples are used to prove that this method can more effectively control the vibration in the servo valve and suppress the interference with shaking table function. Full article
(This article belongs to the Section Machine Design and Theory)
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30 pages, 4492 KB  
Article
Hard Preloaded Duplex Ball Bearing Dynamic Model for Space Applications
by Pablo Riera, Luis Maria Macareno, Igor Fernandez de Bustos and Josu Aguirrebeitia
Machines 2025, 13(7), 581; https://doi.org/10.3390/machines13070581 - 4 Jul 2025
Cited by 1 | Viewed by 966
Abstract
Duplex ball bearings are common components in space satellite mechanisms, and their behaviour impacts the overall performance and reliability of these systems. During rocket launches, these bearings suffer high vibrational loads, making their dynamic response essential for their survival. To predict the dynamic [...] Read more.
Duplex ball bearings are common components in space satellite mechanisms, and their behaviour impacts the overall performance and reliability of these systems. During rocket launches, these bearings suffer high vibrational loads, making their dynamic response essential for their survival. To predict the dynamic behaviour under vibration, simulations and experimental tests are performed. However, published models for space applications fail to capture the variations observed in test responses. This study presents a multi-degree-of-freedom nonlinear multibody model of a hard-preloaded duplex space ball bearing, particularized for this work to the case in which the outer ring is attached to a shaker and the inner ring to a test dummy mass. The model incorporates the Hunt and Crossley contact damping formulation and employs quaternions to accurately represent rotational dynamics. The simulated model response is validated against previously published axial test data, and its response under step, sine, and random excitations is analysed both in the case of radial and axial excitation. The results reveal key insights into frequency evolution, stress distribution, gapping phenomena, and response amplification, providing a deeper understanding of the dynamic performance of space-grade ball bearings. Full article
(This article belongs to the Section Machine Design and Theory)
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21 pages, 2421 KB  
Article
Biosorption and Regeneration Studies for Cu (II) and Cd (II) Removal from Industrial Effluents Using Orange Peel and Composite Adsorbents
by Ahmed A. Bhran, Srinivas Tadepalli, Kasibatla S. R. Murthy and AbdulAziz A. AlGhamdi
Processes 2025, 13(7), 1972; https://doi.org/10.3390/pr13071972 - 22 Jun 2025
Cited by 3 | Viewed by 2523
Abstract
This study investigates the adsorption and desorption efficiencies of Cu (II) and Cd (II) from industrial effluents using orange peel powder and a newly developed mixed adsorbent composed of equal parts of activated charcoal (AC) and bone charcoal (BC). The mixed adsorbent (AC [...] Read more.
This study investigates the adsorption and desorption efficiencies of Cu (II) and Cd (II) from industrial effluents using orange peel powder and a newly developed mixed adsorbent composed of equal parts of activated charcoal (AC) and bone charcoal (BC). The mixed adsorbent (AC + BC) exhibited significantly higher removal efficiencies for both copper and cadmium metal ions compared to orange peel powder. This can be attributed to the high surface area of AC and the negative surface charge of BC, resulting in a synergistic adsorption effect. Batch adsorption experiments were conducted in an orbital shaker at 150–180 rpm for 60 min, followed by thorough rinsing to remove any residual metal ions. The optimal pH for maximum adsorption of Cu (II) and Cd (II) was found to be 6. The effects of adsorbent dosage (ranging from 0.5 to 5 g/L) and contact time (ranging from 15 min to 4 h) on adsorption performance were systematically studied. Regeneration experiments using 0.2 M HCl demonstrated that the adsorption of Cu (II) and Cd (II) on the mixed adsorbent was highly reversible, achieving desorption efficiencies of 90% and 94%, respectively. Notably, Cd (II) consistently exhibited higher desorption rates across all tested dosages. These results confirm the potential of the proposed adsorbent and regeneration strategy for efficient and economical removal of heavy metals from industrial wastewater. Full article
(This article belongs to the Special Issue New Research on Adsorbent Materials in Environmental Protection)
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18 pages, 33781 KB  
Article
New Experimental Single-Axis Excitation Set-Up for Multi-Axial Random Fatigue Assessments
by Luca Campello, Vivien Denis, Raffaella Sesana, Cristiana Delprete and Roger Serra
Machines 2025, 13(7), 539; https://doi.org/10.3390/machines13070539 - 20 Jun 2025
Cited by 1 | Viewed by 601
Abstract
Fatigue failure, generated by local multi-axial random state stress, frequently occurs in many engineering fields. Therefore, it is customary to perform experimental vibration tests for a structural durability assessment. Over the years, a number of testing methodologies, which differ in terms of the [...] Read more.
Fatigue failure, generated by local multi-axial random state stress, frequently occurs in many engineering fields. Therefore, it is customary to perform experimental vibration tests for a structural durability assessment. Over the years, a number of testing methodologies, which differ in terms of the testing machines, specimen geometry, and type of excitation, have been proposed. The aim of this paper is to describe a new testing procedure for random multi-axial fatigue testing. In particular, the paper presents the experimental set-up, the testing procedure, and the data analysis procedure to obtain the multi-axial random fatigue life estimation. The originality of the proposed methodology consists in the experimental set-up, which allows performing multi-axial fatigue tests with different normal-to-shear stress ratios, by choosing the proper frequency range, using a single-axis exciter. The system is composed of a special designed specimen, clamped on a uni-axial shaker. On the specimen tip, a T-shaped mass is placed, which generates a tunable multi-axial stress state. Furthermore, by means of a finite element model, the system dynamic response and the stress on the notched specimen section are estimated. The model is validated through a harmonic acceleration base test. The experimental tests validate the numerical simulations and confirm the presence of bending–torsion coupled loading. Full article
(This article belongs to the Section Machines Testing and Maintenance)
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15 pages, 2225 KB  
Article
The Effect of the Harvest Date on the Possibility of Harvesting by Shaking, Chemical Composition, Color, and Antioxidant Properties of Common Sea Buckthorn Fruit (Hippophae rhamnoides L.)
by Urszula Sadowska and Jacek Słupski
Agronomy 2025, 15(5), 1184; https://doi.org/10.3390/agronomy15051184 - 14 May 2025
Cited by 2 | Viewed by 2131
Abstract
Sea buckthorn (Hippophae rhamnoides L.) fruits were evaluated at three harvest dates, both in terms of ease of harvesting and nutritional value, with attention paid to the visual effect in the form of the color of the harvested fruits. The high values [...] Read more.
Sea buckthorn (Hippophae rhamnoides L.) fruits were evaluated at three harvest dates, both in terms of ease of harvesting and nutritional value, with attention paid to the visual effect in the form of the color of the harvested fruits. The high values of the ratio of fruit bonding strength to individual fruit mass indicated the challenges of effectively harvesting common sea buckthorn using mechanical shakers. However, a decrease in this measure was observed with later harvest dates, as well as differentiation in fruit bonding strength among the tested sea buckthorn cultivars in the seventh and eighth years of plantation growth. As the harvest date was delayed, antioxidant properties and total polyphenol content decreased, while sugar content, individual fruit mass, and fruit length increased. Across the analyzed harvest dates, color differences were more pronounced between cultivars than between individual harvest dates. The numerous interactions observed between sea buckthorn cultivars and harvest dates highlight the need for further research, particularly by increasing the number and frequency of harvest dates for this species. Full article
(This article belongs to the Section Plant-Crop Biology and Biochemistry)
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14 pages, 1174 KB  
Article
Assessment of Alternative Media Viability for Cell Growth Phase in the Lab-Scale Xanthan Pruni Production—Part I
by Isabel Santos Pedone, Fabíola Insaurriaga Aquino, Eduardo dos Santos Macedo Costa, Karine Laste Macagnan, Jéssica da Rosa Porto, Anderson Schwingel Ribeiro, Mariane Igansi Alves, Claire Tondo Vendruscolo and Angelita da Silveira Moreira
Fermentation 2025, 11(4), 191; https://doi.org/10.3390/fermentation11040191 - 3 Apr 2025
Viewed by 939
Abstract
Xanthan is a highly relevant commercial microbial biopolymer. Its production occurs in two steps: the bacterium is cultivated in a nitrogen-rich medium for cell multiplication, and the obtained biomass is used as an inoculum for the polymer production phase. Different media compositions for [...] Read more.
Xanthan is a highly relevant commercial microbial biopolymer. Its production occurs in two steps: the bacterium is cultivated in a nitrogen-rich medium for cell multiplication, and the obtained biomass is used as an inoculum for the polymer production phase. Different media compositions for cell growth were investigated, seeking to reduce or replace the peptone used in the standard medium. Peptone (P), yeast extract (YE), and rice parboiling water (RPW) concentration combinations were tested in cultivating Xanthomonas arboricola pv. pruni 101. A CRD 23 design, performed in a shaker, was used to assess the effects of independent variables on xanthan pruni microbial growth, N consumption, yield, viscosity, pseudoplasticity, and xanthan mineral content. After 24 h an increase in N was observed, without any significant impact on cell growth. Xanthan yield increased as a result of the alternative treatments, with P and YE influencing positively. However, T1, with the lowest levels of P, YE, and RPW increased viscosity and pseudoplasticity of xanthan pruni. RPW increased phosphorus, silicon, calcium, and magnesium, and P and YE increased potassium. These results indicate that partial replacement of P by RPW and YE is an economically viable and sustainable approach for the xanthan pruni production. Full article
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28 pages, 6106 KB  
Article
A Vibration-Based Test Technique to Evaluate the High-Cycle Fatigue Life of Thermal Interface Layers Used in the Electronic Industry
by Alaa Fezai, Anuj Sharma, Wolfgang Müller-Hirsch and André Zimmermann
Appl. Mech. 2025, 6(2), 23; https://doi.org/10.3390/applmech6020023 - 28 Mar 2025
Cited by 1 | Viewed by 1551
Abstract
A testing method is developed to evaluate the acceleration- and strain-based fatigue life of a thermal interface layer in the high-cycle fatigue regime. The methodology adopts vibration-based fatigue testing, where adhesively bonded beams are excited at their resonant frequency under variable amplitude loading [...] Read more.
A testing method is developed to evaluate the acceleration- and strain-based fatigue life of a thermal interface layer in the high-cycle fatigue regime. The methodology adopts vibration-based fatigue testing, where adhesively bonded beams are excited at their resonant frequency under variable amplitude loading using an electrodynamic shaker. Fatigue failure is monitored through shifts in modal frequency and modal damping. Key findings include the identification of a 4% frequency shift as the failure criterion, corresponding to macro-delamination. The thickness of the thermal interface material influences acceleration-based fatigue life, decreasing by a factor of 0.2 when reduced from 0.3 mm to 0.15 mm and increasing by 5.5 when increased to 0.5 mm. Surface quality has a significant impact on both acceleration-based and strain-based fatigue curves. Beams from chemically etched aluminum–magnesium alloy specimens exhibit a sevenfold increase in fatigue life compared to beams from untreated printed circuit boards. Strain-based fatigue life increases with temperature, with a 0.2 reduction at 40 °C and an eightfold increase at 100 °C relative to 23 °C. The first principal strain ε1,rms is validated as a reliable local damage parameter, effectively characterizing fatigue behavior across varying TIM thicknesses. Full article
(This article belongs to the Special Issue Thermal Mechanisms in Solids and Interfaces)
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