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Article

609 Views

18 Pages

Staphylococcal Enterotoxin M Exhibits Thrombin-like Enzymatic Activity

Qian Huang,
Shuang-Hua Luo,
Wan-Fan Tian,
Jun-Ni Tang and
Ji Liu

Biomolecules2025, 15(10), 1357;https://doi.org/10.3390/biom15101357

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24 September 2025

To express and purify staphylococcal enterotoxin M (SEM) using immobilized metal affinity chromatography (IMAC), a signal peptide-truncated (ΔNsp) wild-type SEM (SEMWT) was N-terminally fused in pET-28a(+) to a polyhistidine tag (His6×-)...

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34 Citations

5,940 Views

27 Pages

Novel Feather Degrading Keratinases from Bacillus cereus Group: Biochemical, Genetic and Bioinformatics Analysis

Arwa Ali Almahasheer,
Amal Mahmoud,
Hesham El-Komy,
Amany I. Alqosaibi,
Sultan Aktar,
Sayed AbdulAzeez and
J. Francis Borgio

Microorganisms2022, 10(1), 93;https://doi.org/10.3390/microorganisms10010093

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1 January 2022

In this study, five keratinolytic bacteria were isolated from poultry farm waste of Eastern Province, Saudi Arabia. The highest keratinase activity was obtained at 40–45 °C, pH 8–9, feather concentration 0.5–1%, and using white...

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623 Views

18 Pages

Molecular Characterization and Antiviral Function Against GCRV of Complement Factor D in Barbel Chub (Squaliobarbus curriculus)

Yu Xiao,
Zhao Lv,
Yuling Wei,
Mengyuan Zhang,
Hong Yang,
Chao Huang,
Tiaoyi Xiao and
Yilin Li

Fishes2025, 10(8), 370;https://doi.org/10.3390/fishes10080370

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2 August 2025

The barbel chub (Squaliobarbus curriculus) exhibits remarkable resistance to grass carp reovirus (GCRV), a devastating pathogen in aquaculture. To reveal the molecular basis of this resistance, we investigated complement factor D (DF)—a rate-li...

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