Search Results (332)

Vegetable oils are essential for human nutrition and industrial applications. With growing global demand, increasing oil content in oilseed crops has become a top priority. This review synthesizes recent progress in understanding the genetic, environmental, and molecular mechanisms regulating oil content, and presents biotechnological strategies to enhance oil accumulation in major oilseed crops. Oil biosynthesis is governed by intricate genetic–environmental interactions. Environmental factors and agronomic practices significantly impact oil accumulation dynamics. Quantitative trait loci (QTL) mapping and genome-wide association studies (GWAS) have identified key loci and candidate genes involved in lipid biosynthesis pathways. Transcription factors and epigenetic regulators further fine-tune oil accumulation. Biotechnological approaches, including marker-assisted selection (MAS) and CRISPR/Cas9-mediated genome editing, have successfully generated high-oil-content variants. Future research should integrate multi-omics data, leverage AI-based predictive breeding, and apply precision genome editing to optimize oil yield while maintaining seed quality. This review provides critical references for the genetic improvement and breeding of high- and ultra-high-oil-content varieties in oilseed crops. Full article

With the objective of identifying superior Vernicia montana trees grounded in phenotypic and agronomic traits, this study sought to develop and implement a comprehensive evaluation method which would provide a practical foundation for future clonal breeding initiatives. Using the Vernicia montana propagated from seedling forests grown in the Suxian District of Chenzhou City in southern Hunan Province, we conducted pre-selection, primary selection, and re-selection of Vernicia montana forest stands and took the nine trait indices of single-plant fruiting quantity, single-plant fruit yield, disease and pest resistance, fruit ripening consistency, fruit aggregation, fresh fruit single-fruit weight, fresh fruit seed rate, dry seed kernel rate, and seed kernel oil content rate as the optimal evaluation indexes and carried out cluster analysis and a comprehensive evaluation in order to establish a comprehensive evaluation system for superior Vernicia montana trees. The results demonstrated that a three-stage selection process—consisting of pre-selection, primary selection, and re-selection—was conducted using a comprehensive analytical approach. The pre-selection phase relied primarily on sensory evaluation criteria, including fruit count per plant, tree size, tree morphology, and fruit clustering characteristics. Through this rigorous screening process, 60 elite plants were selected. The primary selection was based on phenotypic traits, including single-plant fruit yield, pest and disease resistance, and uniformity of fruit ripening. From this stage, 36 plants were selected. Twenty plants were then selected for re-selection based on key performance indicators, such as fresh fruit weight, fresh fruit seed yield, dry seed kernel yield, and oil content of the seed kernel. Then the re-selected optimal trees were clustered and analyzed into three classes, with 10 plants in class I, 7 plants in class II, and 3 plants in class III. In class I, the top three superior plants exhibited outstanding performance across key traits: their fresh fruit weight per fruit, fresh fruit seed yield, dry seed yield, and seed kernel oil content reached 41.61 g, 42.80%, 62.42%, and 57.72%, respectively. Compared with other groups, these figures showed significant advantages: 1.17, 1.09, 1.12, and 1.02 times the average values of the 20 reselected superior trees; 1.22, 1.19, 1.20, and 1.08 times those of the 36 primary-selected superior trees; and 1.24, 1.25, 1.26, and 1.19 times those of the 60 pre-selected trees. Fruits counts per plant and the number of fruits produced per plant of the best three plants in class I were 885 and 23.38 kg, respectively, which were 1.13 and 1.18 times higher than the average of 20 re-selected superior trees, 1.25 and 1.30 times higher than the average of 36 first-selected superior trees, and 1.51 and 1.58 times higher than the average of 60 pre-selected superior trees. Class I superior trees, especially the top three genotypes, are suitable for use as mother trees for scion collection in grafting. The findings of this study provide a crucial foundation for developing superior clonal varieties of Vernicia montana through selective breeding. Full article

The goal of this study was to build an understanding of the quality of Idesia polycarpa fruit Maxim from different ecotypes and to identify the best cultivars, with a view to providing a reference and theoretical basis for the selection and cultivation of I. polycarpa. In this study, we systematically evaluated the fruit quality characteristics of five seed sources, namely SH, SG1, GG, HX, and SG2, at four developmental stages, M1-M4, through a principal component analysis, a correlation analysis, and a significance test. Comparisons between the ecotype yielded that GG was significantly better than the other ecotype in oil content (28.7%) and fresh weight per cluster (155.56 g), while HX exhibited higher SOD content (278.18 U/g) and soluble protein content (27.50 mg·g⁻¹), suggesting a higher level of stress tolerance. The results of the correlation analysis showed that POD was significantly negatively correlated with oil content (r = −0.633) and SOD (r = −0.617) activities, indicating that the antioxidant enzyme system may affect oil accumulation. The results of the principal component analysis showed that the cumulative contribution of the first four principal components reached 89.72%, of which principal component 1 mainly reflected yield-related traits, and principal component 2 was significantly correlated with oil content and soluble protein. Through the evaluation and screening of the five ecotypes, we determined that GG can be utilized as a good single plant in the selection and improvement of new cultivars; our findings can provide theoretical support for the selection of good cultivars of I. polycarpa seed in the central region of Henan. Full article

As a unique woody oil crop in China, Camellia oleifera Abel. germplasm resources show significant genetic diversity in Ya’an City. This study measured 60 phenotypic traits (32 quantitative, 28 qualitative) of 302 accessions to analyze phenotypic variation, establish a classification system, and screen high-yield, high-oil germplasms. The phenotypic diversity index for fruit (H’ = 1.36–1.44) was significantly higher than for leaf (H’ = 1.31) and flower (H’ < 1), indicating genetic diversity concentrated in reproductive traits, suggesting potential genetic variability in these traits. Fruit quantitative traits (e.g., single fruit weight CV = 35.37%, fresh seed weight CV = 38.93%) showed high genetic dispersion. Principal component analysis confirmed the fruit factor and economic factor as main phenotypic differentiation drivers. Quantitative traits were classified morphologically, and correlation analysis integrated them into 13 key indicators classified using LSD and range methods. Finally, TOPSIS evaluation selected 10 excellent germplasms like TQ122 and TQ49, with fruit weight, fresh seed yield, and kernel oil content significantly exceeding the population average. This study provides data for C. oleifera DUS test guidelines and proposes a multi-trait breeding strategy, supporting high-yield variety selection and germplasm resource protection. Full article

Soybean meal (SBM) is extensively used as a predominant protein source in animal feed. However, raw soybean cannot be directly utilized in animal feed, due to the presence of the Kunitz trypsin inhibitor (KTi) and the Bowman–Birk protease inhibitor (BBi). These antinutritional factors inhibit the digestive enzymes in animals, trypsin and chymotrypsin, resulting in poor animal performance. To inactivate the activity of protease inhibitors, SBM is subjected to heat processing, a procedure that can negatively impact the soybean protein quality. Thus, it would be beneficial to develop soybean varieties with little or no trypsin inhibitors. In this study, we report on the creation of experimental soybean lines with significantly reduced levels of Bowman–Birk protease inhibitors. RNA interference (RNAi) technology was employed to generate several transgenic soybean lines. Some of these BBi knockdown soybean lines showed significantly lower amounts of both trypsin and chymotrypsin inhibitor activities. Western blot analysis revealed the complete absence of BBi in selected RNAi-derived lines. RNA sequencing (RNAseq) analysis demonstrated a drastic reduction in the seed-specific expression of BBi genes in the transgenic soybean lines during seed development. Confocal fluorescence immunolabeling studies showed that the accumulation of BBi was drastically diminished in BBi knockdown lines compared to wild-type soybeans. The absence of BBi in the transgenic soybean did not alter the overall protein, oil, and sulfur amino acid content of the seeds compared to wild-type soybeans. The seed protein from the BBi knockdown lines were more rapidly hydrolyzed by trypsin and chymotrypsin compared to the wild type, indicating that the absence of BBi enhances protein digestibility. Our study suggests that these BBi knockdown lines could be a valuable resource in order for plant breeders to incorporate this trait into commercial soybean cultivars, potentially enabling the use of raw soybeans in animal feed. Full article

Background: The regulation of oil and protein contents in cottonseed is governed by a complex genetic network. Gaining insight into the mechanisms controlling these traits is necessary for dissecting the formation patterns of cottonseed quality. Method: In this study, Xinluzhong 37 (P₁) and Xinluzhong 51 (P₂) were selected as parental lines for two reciprocal crosses: P₁ × P₂ (F₁) and its reciprocal P₂ × P₁ (F₁′). Each F1 was selfed and backcrossed to both parents to generate the F₂ (F₂′), B₁ (B₁′), and B₂ (B₂′) generations. To assess nutritional traits in hairy (non-delinted) and lint-free (delinted) seeds, two indicators, oil content and protein content, were measured in both seed types. Joint segregation analysis was employed to analyze the inheritance of these traits, based on a major gene plus polygene model. Results: In the orthogonal crosses, the CVs for the four nutritional traits ranged at 2.710–7.879%, 4.086–11.070%, 2.724–6.727%, and 3.717–9.602%. In the reciprocal crosses, CVs ranged at 2.710–8.053%, 4.086–9.572%, 2.724–6.376%, and 3.717–8.845%. All traits exhibited normal or skewed-normal distributions. For oil content in undelinted/delinted seeds, polygenic heritabilities in the orthogonal cross were 0.64/0.52, and 0.40/0.36 in the reciprocal cross. For protein content, major-gene heritabilities in the orthogonal cross were 0.79 (undelinted) and 0.78 (delinted), while those in the reciprocal cross were both 0.62. Conclusions: Oil and protein contents in cottonseeds are quantitative traits. In both orthogonal and reciprocal crosses, oil content is controlled by multiple genes and is shaped by additive, dominance, and epistatic effects. Protein content, in contrast, is largely controlled by two major genes along with minor genes. In the P₁ × P₂ combination, major genes act through additive, dominance, and epistatic effects, while in the P₂ × P₁ combination, their effects are additive only. In both combinations, minor genes contribute through additive and dominance effects. In summary, the oil content in cottonseed is mainly regulated by polygenes, whereas the protein content is primarily determined by major genes. These genetic features in both linted, and lint-free seeds may offer a theoretical foundation for molecular breeding aimed at improving cottonseed oil and protein quality. Full article

Sunflower is one of the four most important oilseed crops in the world, and its edible oil is of high nutritional quality. However, the molecular regulatory mechanism of oil accumulation in sunflowers is still unclear. In this study, we selected two inbred lines with significant differences in oleic acid content and similar agronomic traits: the high oleic acid content (82.5%) inbred line 227 and the low oleic acid content (30.8%) inbred line 228. Sunflower seeds were selected for transcriptome experiments at 10, 20, and 30 days after full bloom (DAFB). There were 21, 225, and 632 differentially expressed genes (DEGs) identified at the three times, respectively. The Gene Ontology (GO) analysis showed that DEGs from two sunflower cultivars at three stages were significantly enriched in the activities of omega-6 fatty acid desaturase and glucosyltransferase. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis found that at 10, 20, and 30 DAFB, DEGs were significantly enriched in the unsaturated fatty acid synthesis pathway, glutathione metabolism pathway, and pyruvate metabolism pathway. Through mapping analysis of GO in the KEGG pathway, it was found that the omega-6 fatty acid desaturase gene FAD6/FAD2, diacylglyceroyltransferase gene DGAT, glycerol-3-phosphate acyltransferase gene GPAT, and long-chain acyl-CoA synthase gene LACS may play important roles in regulating sunflower oleic acid content. Our research provides candidate genes and a research basis for breeding high oleic sunflowers. Full article

In the current context of environmental concerns and the search for sustainable food solutions, this study investigated the valorization of Luffa cylindrica seed press cake, a waste byproduct from oil extraction, as a functional ingredient for meat substitute formulations. The research systematically characterized the functional and bioactive properties of L. cylindrica seed press cake powder (LP) and its blends with tapioca flour (TF) at ratios of 30–70%. Techno-functional analyses included: hydration properties (water holding capacity, water absorption capacity, water absorption index, water solubility index, swelling power, oil absorption capacity); rheological characteristics; bioactive profiling through antioxidant assays (DPPH, ABTS, FRAP); and reducing sugar content determination. Meat substitute formulations were developed using an LP30/TF70 blend combined with coral lentils, red beet powder, and water, followed by a sensory evaluation and storage stability assessment. Pure L. cylindrica powder exhibited the highest water holding capacity (3.62 g H₂O/g) and reducing sugar content (8.05 mg GE/g), while tapioca flour showed superior swelling properties. The blends demonstrated complementary functional characteristics, with the LP30/TF70 formulation selected for meat substitute development based on optimal textural properties. The sensory evaluation revealed significant gender differences in acceptance, with women rating the product substantially higher than men across all attributes. The study successfully demonstrated the feasibility of transforming agricultural waste into a valuable functional ingredient, contributing to sustainable food production and representing the first comprehensive evaluation of L. cylindrica seed press cake for food applications. However, the study revealed limitations, including significant antioxidant loss during thermal processing (80–85% reduction); a preliminary sensory evaluation with limited participants showing gender-dependent acceptance; and a reliance on locally available tapioca flour, which may limit global applicability. Future research should focus on processing optimization to preserve bioactive compounds, comprehensive sensory studies with diverse populations, and an investigation of alternative starch sources to enhance the worldwide implementation of this valorization approach. Full article

Brassica napus is one of the most important oil crops. Rapid breeding of excellent genotypes is an important aspect of breeding. As a cutting-edge and reliable technique, genome-wide selection (GS) has been widely used and is influenced by many factors. In this study, ten phenotypic traits of two populations were studied, including oleic acid (C18:1), linoleic acid (C18:2), linolenic acid (C18:3), glucosinolate (GSL), seed oil content (SOC), and seed protein content (SPC), silique length (SL), days to initial flowering (DIF), days to final flowering (DFF), and the flowering period (FP). The effects of different GS models, marker densities, population designs, and the inclusion of nonadditive effects, trait-specific SNPs, and deleterious mutations on GS were evaluated. The results highlight the superior prediction accuracy (PA) under the RF model. Among the ten traits, the PA of glucosinolate was the highest, and that of linolenic acid was the lowest. At the same time, 5000 markers and a population of 400 samples, or a training population three times the size of an applied breeding population, can achieve optimal performance for most traits. The application of nonadditive effects and deleterious mutations had a weak effect on the improvement of traits with high PA but was effective for traits with low PA. The use of trait-specific SNPs can effectively improve the PA, especially when using markers with p-values less than 0.1. In addition, we found that the PA of traits was significantly and positively correlated with the number of markers, according to p-values less than 0.01. In general, based on the associated population, a GS system suitable for B. napus was established in this study, which can provide a reference for the improvement of GS and is conducive to the subsequent application of GS in B. napus, especially in the aspects of model selection of GS, the application of markers, and the setting of population sizes. Full article

Tocopherols, due to their antioxidant properties, are valuable compounds in the food and pharmaceutical industries. The present study compares the glyceride oil content and tocopherol profile of seeds of 49 plant species from 39 families of Bulgarian wild flora to identify their potential industrial and nutritional applications. The oils were extracted using the Soxhlet apparatus, and the tocopherol and tocotrienol composition was established by high-performance liquid chromatography (HPLC). Hierarchical cluster analysis was performed to group the plants based on their tocopherol profile. The results show high variability in the content of glyceride oils (ranging from 0.5% to 40.6%) and tocopherol profiles among species, even among plants within the same family. Four clusters were identified, each of which was characterized by the dominance of one of the tocopherol or tocotrienol isomers, i.e., α-tocopherol or γ-tocopherol, and reflected the chemical diversity of the examined plants. The statistical analysis confirmed that tocopherols and tocotrienols are significant factors influencing cluster grouping. The results reflect natural variability among species grown under field conditions, influenced by both genetic and environmental factors. This study provides valuable preliminary information for identifying wild species with promising tocopherol profiles for future functional research. Full article

Phytosterols (PS) have specific oxidation rules in different lipid media. After oxidation, PS will form oxidation products, which has potential physiological toxicity to the human body. Camellia seed oil (CSO) is a unique emerging edible oil in China. This oil has a fatty acid composition similar to olive oil, in which oleic acid is dominant. In order to solve the thermal oxidation of PS in CSO at high temperature (180 °C), we studied its antioxidant strategy by evaluating different antioxidants. Four antioxidants—BHA, TBHQ, epigallocatechin gallate (EGCG), and α-tocopherol (VE)—along with one synergist, citric acid (CA), were selected and used in this study. The antioxidant effects of different combinations (single antioxidant, single antioxidant + CA, mixed antioxidant, mixed antioxidant + CA) were compared. After 180 min of heating, the PS and phytosterols oxidation products (7α-hydroxy-, 7β-hydroxy-, 5α,6α-epoxy-, 5β,6β-epoxy-, 7-keto-, and trihydroxy-PS) were estimated by GC-MS. Through comparative analysis, the results showed that the combination of mixed antioxidants and CA had the best antioxidant effect, and the inhibition rate of VE + TBHQ +CA was as high as 42%, which had a breakthrough significance for stabilizing the thermal oxidation of PS in camellia seed oil. At the same time, it also provides a valuable reference for ensuring the edible safety of camellia seed oil in Chinese food heating habits. Full article

The aqueous enzymatic extraction (AEE) of oil from Thevetia peruviana seeds was studied. The oil extraction yields obtained by treatment with cellulase, Alcalase, and Viscozyme L were compared, finding that the treatment with Alcalase at pH 6 and 50 °C gave the highest yield (63.07%), so it was selected for the optimization of the oil extraction process through a central composite design and a response surface methodology. An oil extraction yield of 78.22% was achieved under the optimum conditions (2% of Alcalase, 4 h of incubation, 250 rpm, and a solid-to-liquid ratio of 1:4.5). The predominant fatty acids in the oil obtained by both AEE and standard solvent extraction (SE) were oleic, linoleic, stearic, and palmitic acids. However, AEE produced an oil presenting lower acidity and peroxide values and greater oxidative stability, indicating that this method produces an oil with better quality. Scanning electron microscopy images of Thevetia peruviana seeds showed a structural alteration of their cell wall caused by AEE, which allowed the release of oil from this seed in the aqueous medium. The results suggest that AEE could be an excellent alternative in terms of efficiency, oil quality, and environmental friendliness for the extraction of this oil. Full article

Food security remains one of the most critical global challenges of the 21st century. Traditional tree crops domesticated by indigenous people have the potential to increase food security and improve the livelihoods of smallholders in developing countries. However, the nut characteristics of many traditional tree crop species in the tropics are poorly understood. In particular, physical and chemical characteristics are important to explore when selecting trees to commercialise. Three species, Barringtonia procera, B. edulis, and B. nova-hiberniae, have a long history of traditional use and domestication in Pacific Island countries. The aim of this study was to explore the physical and chemical characteristics of Barringtonia spp. in three Pacific countries: Solomon Islands, Vanuatu, and Fiji. There were significant differences in kernel weight, oil concentration, and fatty acid concentration among the countries. The kernel weight was significantly higher in Solomon Islands compared with those in Vanuatu and Fiji (9.65 g, 7.61 g, and 5.64 g, respectively). Average kernel weight in Fiji was well above 3 g, which indicated that processing could be commercially viable. The total oil concentration was significantly higher in Vanuatu and Solomon Islands than Fiji, with average concentrations of 38.96% in Solomon Islands, 47.11% in Vanuatu, and 26.20% in Fiji. Barringtonia spp. exhibited high concentrations of unsaturated fatty acids, similar to other tropical nuts, which suggests that it may be a potential healthy oil for human consumption. Notably, kernel size, oil concentration, and fatty acid composition varied geographically, potentially due to climatic differences and historical seed transfer. Our study demonstrated the potential of Barringtonia to be commercialised to enhance food and nutrition security and provide a guide for cultivar selection. Full article

Camelina sativa (L.) Crantz, a native European oilseed crop of the Brassicaceae family, is notable for its short life cycle, making it well-suited for crop rotation and diversification. Its seeds contain a high content of oil (36–47%) that is rich in polyunsaturated fatty acids (PUFAs) such as alpha-linolenic acid (ALA, C18:3, Ω-3) and linoleic acid (LA, C18:2, Ω-6). This oil has diverse industrial applications, including low-emission biofuels, animal feed, pharmaceuticals, biolubricants, bioplastics, and cosmetics. We analyzed the expression of seven key enzymes involved in fatty acid biosynthesis across nine C. sativa accessions at three stages of silique development using highly efficient qRT-PCR assays designed for the target genes and a normalizing control. Our detailed expression analysis revealed significant variation across varieties, with only the gene FAB2c exhibiting genotype-independent expression, indicating a constitutive and essential role in monounsaturated fatty acid (MUFA) biosynthesis. Other genes showed significant interactions between the variety and developmental stage, highlighting the combined influences of genetic background and silique maturation on gene regulation. V18 emerges as particularly promising, exhibiting elevated expression of genes linked to PUFA and VLCFA biosynthesis—traits of significance for food, biofuel, and industrial applications. These findings, together with the developed qRT-PCR assays, provide valuable tools for selecting Camelina varieties with optimized genetic profiles, highlighting the potential of harnessing natural transcriptional diversity for crop improvement. Full article

Skin aging is a complex process influenced by several factors, including UV exposure, environmental stressors, and lifestyle choices. The demand for effective, natural skincare products has driven research into plant-based oils rich in bioactive compounds. Rosehip oil has garnered attention for its high content of carotenoids, phenolics, and antioxidants, which are known for their anti-aging, photoprotective, and skin-rejuvenating properties. Despite the growing interest in rosehip oil, limited studies have investigated its efficacy on human skin using advanced imaging technologies. This study aims to fill this gap by evaluating the efficacy of cold-pressed Rosa canina seed oil on facial skin characteristics, specifically wrinkles, ultraviolet (UV) spot reduction, and erythema mitigation, using imaging technologies (the VISIA analysis system). Seed oil pressed from R. canina collected from the Băișoara area of Cluj County has been selected for this study due to its high carotenoid, phenolic, and antioxidant contents. The oil has also been analyzed for the content of individual carotenoids (i.e., lutein, lycopene, β Carotene, and zeaxanthin) using HPLC-DAD (High-Performance Liquid Chromatography—Diode Array Detector), along with lutein and zeaxanthin esters and diesters. After the preliminary screening of multiple Rosa species for carotenoid, phenolic, and antioxidant contents, the R. canina sample with the highest therapeutic potential was selected. A cohort of 27 volunteers (aged 30–65) underwent a five-week treatment protocol, wherein three drops of the selected rosehip oil were topically applied to the face daily. The VISIA imaging was conducted before and after the treatment to evaluate changes in skin parameters, including the wrinkle depth, UV-induced spots, porphyrins, and texture. Regarding the bioactivities, rosehip oil showed a significant total carotenoids content (28.398 μg/mL), with the highest levels in the case of the β-carotene (4.49 μg/mL), lutein (4.33 μg/mL), and zexanthin (10.88 μg/mL) contents. Results indicated a significant reduction in mean wrinkle scores across several age groups, with notable improvements in individuals with deeper baseline wrinkles. UV spots also showed visible declines, suggesting ideal photoprotective and anti-pigmentary effects attributable to the oil’s high vitamin A and carotenoid content. Porphyrin levels, often correlated with bacterial activity, decreased in most subjects, hinting at an additional antimicrobial or microbiome-modulatory property. However, skin responses varied, possibly due to individual differences in skin sensitivity, environmental factors, or compliance with sun protection. Overall, the topical application of R. canina oil appeared to improve the facial skin quality, reduce the appearance of age-related markers, and support skin health. These findings reinforce the potential use of rosehip oil in anti-aging skincare formulations. Further long-term, large-scale studies are warranted to refine dosing regimens, investigate mechanisms of action, and explore synergistic effects with other bioactive compounds. Full article