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25 pages, 2447 KB  
Article
Niche Differentiation Characteristics of Phytoplankton Functional Groups in Arid Regions of Northwest China Based on Machine Learning
by Long Yun, Fangze Zi, Xuelian Qiu, Qi Liu, Jiaqi Zhang, Liting Yang, Yong Song and Shengao Chen
Biology 2025, 14(11), 1564; https://doi.org/10.3390/biology14111564 - 7 Nov 2025
Viewed by 149
Abstract
This study investigates the distribution patterns, interspecific relationships, and community stability mechanisms of phytoplankton functional groups, aiming to elucidate the ecological processes that drive phytoplankton communities in aquatic ecosystems of arid regions. We conducted seasonal sampling from 2023 to 2024 at four auxiliary [...] Read more.
This study investigates the distribution patterns, interspecific relationships, and community stability mechanisms of phytoplankton functional groups, aiming to elucidate the ecological processes that drive phytoplankton communities in aquatic ecosystems of arid regions. We conducted seasonal sampling from 2023 to 2024 at four auxiliary reservoirs in the Tarim River Basin, namely Shangyou Reservoir (SY), Shengli Reservoir (SL), Duolang Reservoir (DL), and Xinjingzi Reservoir (XJZ). In recent years, researchers have grouped phytoplankton into functional groups based on their shared morphological, physiological, and ecological characteristics—with these three types of traits serving as the core criteria for distinguishing different functional groups. A total of 18 functional groups were identified from the phytoplankton collected across four seasons, among which eight (A, D, H1, L0, M, MP, P, and S1) are dominant. Redundancy Analysis (RDA) indicated that environmental factors such as pH, electrical conductivity (COND), and dissolved oxygen (DO) are key driving factors affecting phytoplankton functional groups. Interspecific association analysis showed that the phytoplankton communities in DL, SL, and XJZ reservoirs were dominated by positive associations, reflecting stable community structures that are less prone to drastic fluctuations under stable environmental conditions. In contrast, the SY Reservoir was dominated by negative associations, indicating that it is in the early stage of succession with an unstable community. This may be related to intense human disturbance to the reservoir and its role in replenishing the Tarim River, which leads to significant water level fluctuations. The results of the Chi-square test and Pearson correlation analysis showed consistent trends but also differences: constrained by the requirement for continuous normal distribution, Pearson correlation analysis identified more pairs of negative associations, reflecting its limitations in analysing clumped-distributed species. Random forest models further indicated that functional groups M, MP, L0, and S1 are the main positive drivers of interspecific relationships. Among them, the increase in S1 can promote the growth of functional groups dominated by Navicula sp. and Chroococcus sp. by reducing resource competition. Conversely, the expansion of functional group H1 inhibits other groups, which is related to its adaptive strategy of resisting photo-oxidation in eutrophic environments. This study reveals the patterns of interspecific interactions and stability mechanisms of phytoplankton functional groups in arid-region reservoirs, providing a scientific basis for the management and conservation of aquatic ecosystems in similar extreme environments. Full article
(This article belongs to the Special Issue Wetland Ecosystems (2nd Edition))
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17 pages, 1980 KB  
Article
Occurrence Characteristics and Ecological Risk Assessment of Microplastics in Aquatic Environments of Cascade Reservoirs Along the Middle-Lower Han River
by Ruining Zhang, Ziwei Guo, Li Lin, Xiong Pan, Yu Gao and Yuqiang Liu
Water 2025, 17(19), 2793; https://doi.org/10.3390/w17192793 - 23 Sep 2025
Cited by 1 | Viewed by 544
Abstract
The presence and accumulation of microplastics (MPs) in riverine waters have been widely documented. The sustained operation of cascade reservoirs has altered the retention characteristics of MPs in the Han River basin. In this study, the composition, sources, and ecological risks of MPs [...] Read more.
The presence and accumulation of microplastics (MPs) in riverine waters have been widely documented. The sustained operation of cascade reservoirs has altered the retention characteristics of MPs in the Han River basin. In this study, the composition, sources, and ecological risks of MPs in the water column and sediments of the Han River mainstream across different periods were investigated. Results showed that the MP abundances in the water column and sediments were higher during the flood season than in the non-flood season. Additionally, MPs in the water column exhibited an increasing trend along the operational sequence of cascade reservoirs. During the flood season, polyamide (PA), polyethylene (PE), and polypropylene (PP) were the dominant MP types in the water column, while polycarbonate (PC) and PP prevailed in sediments. In the non-flood season, polyethylene terephthalate (PET) was the dominant MP type in the water column, whereas PC and PET dominated in sediments. Overall, the distribution characteristics of MPs conformed to the “upstream input-reservoir accumulation-downstream output” pattern. The pollution risk of MPs in both the water column and sediments ranged from low to moderate. These findings provide a basis for exploring the impacts of cascade reservoir operation on the characteristics of MP in water and sediments. Future research will focus on migration mechanisms of MP under the joint operation of cascade reservoirs. Full article
(This article belongs to the Section Water Quality and Contamination)
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22 pages, 6778 KB  
Article
Detection of Antibiotic-Resistant Escherichia coli in the Upper Citarum River Using a β-D-Glucuronidase Method
by Siska Widya Dewi Kusumah, Mochinaga Katsuya, Rifky Rizkullah Fahmi, Peni Astrini Notodarmojo, Ahmad Soleh Setiyawan, Hisashi Satoh and Herto Dwi Ariesyady
Water 2025, 17(18), 2791; https://doi.org/10.3390/w17182791 - 22 Sep 2025
Viewed by 718
Abstract
Background: Polluted rivers may become reservoirs of antibiotic-resistant Escherichia coli (AREc), raising concerns about environmental health. While monitoring is crucial for recognizing their incidence and evaluating mitigation solutions, current approaches are limited due to high costs, labor-intensive methods, and a lack of standardized [...] Read more.
Background: Polluted rivers may become reservoirs of antibiotic-resistant Escherichia coli (AREc), raising concerns about environmental health. While monitoring is crucial for recognizing their incidence and evaluating mitigation solutions, current approaches are limited due to high costs, labor-intensive methods, and a lack of standardized indicators. This study aims to identify the priority AREc as the monitoring target and evaluate the applicability of the β-glucuronidase enzyme detection method (MPR Method) as an alternative rapid method for profiling AREc in the Upper Citarum River. Methods: River water sampling was conducted along the river during two periods with varying rainfall levels. Total Escherichia coli (TEc) and twelve types of antibiotic-resistant Escherichia coli (AREc) were measured simultaneously by the Agar Method and the β-D-Glucuronidase detection (MPR Method). Results: Statistical data analyses indicate that Total Escherichia coli (TEc) concentrations in the Upper Citarum River increase during periods of higher rainfall (𝓍 = 2558 ± 360 CFU/mL). Erythromycin-resistant Escherichia coli dominates in both periods (Period I 𝓍 = 57.6 ± 25.9%, Period II 𝓍 = 49.96 ± 29.5%). However, tetracycline-resistant Escherichia coli and Extended-Spectrum β-lactamase-producing Escherichia coli (ESBL-Ec) are the most suitable indicators for AREc concentration due to their consistency and correlation with other AREc types. The MPR method achieved an accuracy of up to 87.2%, a sensitivity of 67.4%, and a specificity of 94%. Conclusion: The MPR Method was considered a better alternative for the AREc screening method, particularly in a high bacterial load aquatic environment. Full article
(This article belongs to the Section Water Quality and Contamination)
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17 pages, 10795 KB  
Article
Lithofacies Characteristics of Point Bars and Their Control on Incremental Oil Recovery Distribution During Surfactant–Polymer Flooding: A Case Study from the Gudao Oilfield
by Xilei Liu, Changchun Guo, Qi Chen, Minghao Zhao and Yuming Liu
Energies 2025, 18(17), 4703; https://doi.org/10.3390/en18174703 - 4 Sep 2025
Viewed by 773
Abstract
Meandering river point bar sand bodies, serving as critical reservoir units, exhibit significant lithofacies heterogeneity that governs remaining oil distribution patterns. Taking the Guantao Formation in the Gudao Oilfield as an example, this study integrates core observation, pore-throat structure characterization, and numerical simulation [...] Read more.
Meandering river point bar sand bodies, serving as critical reservoir units, exhibit significant lithofacies heterogeneity that governs remaining oil distribution patterns. Taking the Guantao Formation in the Gudao Oilfield as an example, this study integrates core observation, pore-throat structure characterization, and numerical simulation to reveal lithofacies characteristics of point bar sand bodies and their controlling mechanisms on incremental oil recovery distribution during surfactant–polymer (SP) flooding. The results demonstrate that point bar lithofacies display planar grain-size fining from concave to convex banks, with vertical upward-fining sequences (point bar medium sandstone facies → fine sandstone facies → siltstone facies). Physical property variations among lithofacies lead to remaining oil enrichment in relatively low-permeability portions of fine sandstone facies and low-permeability siltstone facies after waterflooding. SP flooding significantly enhances remaining oil mobilization through a “lithofacies-controlled percolation—chemical synergy” coupling mechanisms. The petrophysical heterogeneity formed by vertical lithofacies assemblages in the reservoir directly governs the targeted zones of chemical agent action (with interfacial tension reduction preferentially occurring in high-permeability lithofacies, while viscosity control dominates sweep enhancement in low-permeability lithofacies). This results in a distinct spatial differentiation of the incremental oil recovery, characterized by a spindle-shaped sweep improvement zone and a dam-type displacement efficiency enhancement zone. Full article
(This article belongs to the Special Issue Enhanced Oil Recovery: Numerical Simulation and Deep Machine Learning)
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23 pages, 9816 KB  
Article
Improving Recovery Mechanism Through Multi-Well Water and Gas Injection in Underground River Reservoirs
by Shenghui Yue, Wanjiang Guo, Mingshan Ding and Aifen Li
Processes 2025, 13(9), 2743; https://doi.org/10.3390/pr13092743 - 27 Aug 2025
Viewed by 607
Abstract
Underground river reservoirs are dominated by large-scale elongated caves and are typical fractured–vuggy carbonate reservoirs. This paper established physical models of underground river reservoirs with different filling modes. We first conducted bottom water flooding experiments and then studied multi-well, alternating water flooding and [...] Read more.
Underground river reservoirs are dominated by large-scale elongated caves and are typical fractured–vuggy carbonate reservoirs. This paper established physical models of underground river reservoirs with different filling modes. We first conducted bottom water flooding experiments and then studied multi-well, alternating water flooding and gas injection. The remaining oil distribution patterns and key factors under different filling modes and well locations were studied to clarify the recovery-improvement mechanisms of multi-well water and gas injection. The results show that the remaining oil after bottom water flooding can be categorized into the following five types: “insufficient well control remaining oil”, “attic remaining oil”, “bypass remaining oil”, “residual oil in filling medium”, and “shielded oil in filling medium”. Early water injection effectively recovers “insufficient well control remaining oil”, “bypass remaining oil”, and “residual oil in filling medium”. Gas injection targets included “attic remaining oil”. Late water injection can further improve recovery. When the cave is partially filled, there exists a large amount of “shielded oil in filling medium” that is difficult to recover, reducing recovery by 27% compared to unfilled cases. This study clarified the remaining oil distribution laws and water–gas flooding mechanisms for underground river reservoirs, providing guidance for efficient development. Full article
(This article belongs to the Special Issue Advances in Unconventional Reservoir Development and CO2 Storage)
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29 pages, 11185 KB  
Article
Assessment of the Volume, Spatial Diversity, Functioning, and Structure of Sediments in Water Bodies Within the Słubia River Catchment (Myślibórz Lakeland, Poland)
by Witold Jucha, Aleksandra Bobrek, Weronika Ceglarek, Piotr Cybul, Izabela Grabiec, Nikola Kachnowicz, Michał Kijowski, Natalia Konderak, Paulina Mareczka, Daniel Okupny, Zofia Sotek, Izabela Rysak and Piotr Trzepla
Water 2025, 17(17), 2530; https://doi.org/10.3390/w17172530 - 26 Aug 2025
Viewed by 1222
Abstract
Water reservoirs play a crucial role in the environment in many aspects: hydrology, geochemistry, sediment lithology, geo- and biodiversity, landscape, etc. First of all, it is necessary to have accurate information about the spatial distribution of these objects in a given area to [...] Read more.
Water reservoirs play a crucial role in the environment in many aspects: hydrology, geochemistry, sediment lithology, geo- and biodiversity, landscape, etc. First of all, it is necessary to have accurate information about the spatial distribution of these objects in a given area to assess their size and functioning. Maps and contemporary spatial databases are often incomplete or outdated, especially in regard to small objects, of variable surface area and condition. This article uses the following approach: high-resolution terrain models derived from airborne laser scanning (ALS) were used for visual interpretation of extensive, flat depressions representing water body basins, thus determining the total number of objects, and classifying them as kettle holes, lakes, ponds, and other types of reservoirs (e.g., overbank basins, oxbow lakes). Using an aerial orthophotomap, the objects were subsequently verified as to how many basins are currently occupied by water bodies. The next step was to determine a number of topographic and morphometric parameters for each object in order to assess their functioning conditions. For selected objects, the assessment was expanded to include a geochemical and lithological analysis of the sediments. The study was conducted in the catchment of the Słubia River (136 km2), located in Central Europe, in northwestern Poland. In the Słubia catchment, a total of 931 water body basins were mapped. The dominant forms are kettle holes (<1 ha), representing nearly 80% of all objects. At present, kettle holes are largely devoid of water bodies and subject to a strong human impact. In addition to those, 118 lake basins were identified (>1 ha, the largest being Lake Morzycko, 360 ha), half of which are occupied by water reservoirs. Ponds and other reservoirs were represented by 37 and 47 objects, respectively. From the perspective of contemporary sediment-forming processes in the documented sedimentary basins, the most favorable conditions for biogenous sediment accumulation exist in the catchments of the upper and medium courses of the Słubia River valley. Although the lithological diversity and thickness of individual sediment types in the Słubia catchment represent local features, they corroborate the results of previous telmatologic research conducted in Myślibórz Lakeland. Full article
(This article belongs to the Section Water Erosion and Sediment Transport)
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21 pages, 3366 KB  
Article
Patterns of Genetic and Clonal Diversity in Myriophyllum spicatum in Streams and Reservoirs of Republic of Korea
by Eun-Hye Kim, Kang-Rae Kim, Mi-Hwa Lee, Jaeduk Goh and Jeong-Nam Yu
Plants 2025, 14(17), 2648; https://doi.org/10.3390/plants14172648 - 26 Aug 2025
Viewed by 727
Abstract
Myriophyllum spicatum is a globally distributed aquatic plant capable of sexual and clonal reproduction. Despite its ecological importance and biochemical potential, studies on its genetic and clonal structure in freshwater systems throughout South Korea remain limited. We investigated the genetic and clonal diversity [...] Read more.
Myriophyllum spicatum is a globally distributed aquatic plant capable of sexual and clonal reproduction. Despite its ecological importance and biochemical potential, studies on its genetic and clonal structure in freshwater systems throughout South Korea remain limited. We investigated the genetic and clonal diversity of M. spicatum using 30 newly developed microsatellite markers across 120 individuals from six freshwater systems in South Korea. Overall, 148 alleles were identified, with an average polymorphism information content value of 0.530. Clonal diversity differed among populations, with the genotypes to individuals (G/N) ratio ranging from 0.200 to 1.000. Bottlenecks and clonal dominance were observed in riverine populations. High genetic differentiation (mean FST = 0.556) indicated limited gene flow, and STRUCTURE analysis revealed six distinct genetic clusters. No significant correlation was found between genetic and geographic distance, suggesting possible seed dispersal by waterfowl, particularly between adjacent populations. Genetic structure was shaped by habitat type, disturbance intensity, and reproductive strategy. Stable reservoir habitats favored sexual reproduction and higher genetic diversity, whereas disturbed river systems showed clonal dominance and reduced variation. These findings provide essential genetic insights for conservation planning and sustainable management of aquatic plant resources. Full article
(This article belongs to the Special Issue Plant Genetic Diversity and Molecular Evolution)
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20 pages, 11244 KB  
Article
Pore Structure Characteristics and Genesis of Low-Permeability Sandstone Reservoirs in the Eocene Wenchang Formation, Huizhou Sag, Pearl River Mouth Basin, Northern South China Sea
by Guanliang Zhang, Jiancheng Niu, Zhiling Yang, Qibiao Zang, Qingyu Zhang, Haoxian Liu, Qamar Yasin and Mengdi Sun
J. Mar. Sci. Eng. 2025, 13(9), 1620; https://doi.org/10.3390/jmse13091620 - 25 Aug 2025
Viewed by 609
Abstract
Porosity and permeability are critical parameters in petroleum exploration and development. The relationship between pore structure and permeability in near-source reservoirs is more closely correlated than in other types of reservoirs. This study investigates the pore structure and formation processes of low-permeability sandstone [...] Read more.
Porosity and permeability are critical parameters in petroleum exploration and development. The relationship between pore structure and permeability in near-source reservoirs is more closely correlated than in other types of reservoirs. This study investigates the pore structure and formation processes of low-permeability sandstone reservoirs in the Wenchang Formation, Huizhou Depression, Pearl River Mouth Basin (Northern South China Sea). We collected ten core samples of low-permeability sandstone reservoirs at various depths from the key well (A). Multiple analytical techniques were employed, including mercury intrusion capillary pressure (MICP), constant velocity mercury injection (CMI), Wood’s metal impregnation (WM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and quantitative evaluation of minerals via scanning electron microscopy (QEMSCAN). Pore-throat types were classified using fractal theory, followed by analyzing the physical and structural characteristics of interconnected pore-throat reservoir systems. This study examined the impact of various pore types on the physical properties of reservoirs, providing a comprehensive classification and characterization of pore structures in low-permeability sandstone reservoirs. Our findings provide significant insights and recommendations for future developmental initiatives in this region. Full article
(This article belongs to the Special Issue Advances in Offshore Oil and Gas Exploration and Development)
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20 pages, 2584 KB  
Article
Remote Sensing Assessment of Trophic State in Reservoir Tributary Embayments Based on Multi-Source Data Fusion
by Yangjie Shi, Jingqiao Mao, Xinbo Liu, Dinghua Meng, Jianing Zhu, Huan Gao and Kang Wang
Remote Sens. 2025, 17(16), 2886; https://doi.org/10.3390/rs17162886 - 19 Aug 2025
Viewed by 856
Abstract
Monitoring water quality in narrow tributary bays of large river-type reservoirs is hindered by sparse sampling and cloud-limited imagery. This study develops a Trophic State Index (TSI) inversion for Xiangxi Bay, a major tributary bay of the Three Gorges Reservoir, using [...] Read more.
Monitoring water quality in narrow tributary bays of large river-type reservoirs is hindered by sparse sampling and cloud-limited imagery. This study develops a Trophic State Index (TSI) inversion for Xiangxi Bay, a major tributary bay of the Three Gorges Reservoir, using Landsat data and a backpropagation (BP) neural network, with hyperparameters tuned via a grid search algorithm (GSA). Environmental drivers such as water temperature, solar radiation, and photosynthetically active radiation were combined with Landsat spectral bands. Eleven sites measured monthly in 2009 yielded 98 samples after preprocessing, and training achieved R2 = 0.94. Predictions for 2009 show clear spatiotemporal heterogeneity: those for April and September (TSI = 48–59) exceeded those for July and October (46–56), with mid–lower reaches (52–59) being higher than mid–upper reaches (47–54). Out-of-period predictions for April/June 2019 and August/November 2020 were consistent with seasonal expectations, with higher spring–summer TSIs (2019: 50–57; 2020 August: 45–55) than in November 2020 (37–47). Key limitations include the small sample size, cloud-related data gaps, and sensitivity to evolving reservoir operations. This framework demonstrates a practical route to the satellite-based monitoring and mapping of trophic status in narrow reservoir tributaries. Full article
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15 pages, 7282 KB  
Article
Spatiotemporal Patterns and Atmospheric Drivers of Anomalous Precipitation in the Taihu Basin, Eastern China
by Jingwen Hu, Jian Zhang, Abhishek, Wenpeng Zhao, Chuanqiao Zhou, Shuoyuan Liang, Biao Long, Ying Xu and Shuping Ma
Water 2025, 17(16), 2442; https://doi.org/10.3390/w17162442 - 18 Aug 2025
Viewed by 983
Abstract
This study investigates anomalous precipitation patterns in the Taihu Basin, located in the Yangtze River Delta of eastern China, using high-resolution daily data from 1960 to 2019. Leveraging a deep learning autoencoder and self-organizing map, three spatially distinct types are identified—north type (72%), [...] Read more.
This study investigates anomalous precipitation patterns in the Taihu Basin, located in the Yangtze River Delta of eastern China, using high-resolution daily data from 1960 to 2019. Leveraging a deep learning autoencoder and self-organizing map, three spatially distinct types are identified—north type (72%), south type (19.7%), and center type (8.3%). The north type exhibits a pronounced upward trend (+0.11 days/year, p < 0.05), indicating intensifying extreme rainfall under climate warming, while the south type displays a bimodal temporal structure, peaking in early summer and autumn. Composite analyses reveal that these patterns are closely associated with the westward extension of the Western North Pacific Subtropical High (WNPSH), meridional shifts of the East Asian Westerly Jet (EAJ), low-level moisture convergence, and SST–OLR anomalies. For instance, north-type events often coincide with strong anticyclonic anomalies and enhanced moisture transport from the Northwest Pacific and South China Sea, forming favorable convergence zones over the basin. For flood management in the Taihu Basin, the identified spatial patterns, particularly the bimodal south type, have clear implications. Their strong link to specific circulation features enables certain flood-prone scenarios to be anticipated 1–2 seasons in advance, supporting proactive measures such as reservoir scheduling. Overall, this classification framework deepens the understanding of atmospheric patterns associated with flood risk and provides practical guidance for storm design and adaptive flood risk management under a changing climate. Full article
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18 pages, 6368 KB  
Article
Research on the Genesis Mechanism of Hot Springs in the Middle Reaches of the Wenhe River
by Cheng Xue, Nan Xing, Zongjun Gao, Yiru Niu and Dongdong Yang
Water 2025, 17(16), 2431; https://doi.org/10.3390/w17162431 - 17 Aug 2025
Viewed by 876
Abstract
This study investigates geothermal clusters in the middle reaches of the Dawen River Basin, focusing on the developmental characteristics and genetic mechanisms of typical geothermal water exposures at key sites, including Daidaoan (Taishan), Qiaogou (Culai Town), and Anjiazhuang (Feicheng). Utilizing hydrogeochemical and environmental [...] Read more.
This study investigates geothermal clusters in the middle reaches of the Dawen River Basin, focusing on the developmental characteristics and genetic mechanisms of typical geothermal water exposures at key sites, including Daidaoan (Taishan), Qiaogou (Culai Town), and Anjiazhuang (Feicheng). Utilizing hydrogeochemical and environmental isotope analyses, we identify a dual groundwater recharge mechanism: (1) rapid infiltration via preferential flow through fissure media and (2) slow seepage with evaporative loss along gas-bearing zones. Ion sources are influenced by water–rock interactions and positive cation exchange. The hydrochemical types of surface water and geothermal water can be divided into five categories, with little difference within the same geothermal area. The thermal reservoir temperatures range from 53.54 to 101.49 °C, with the Anjiazhuang and Qiaogou geothermal areas displaying higher temperatures than the Daidaoan area. Isotope calculations indicate that the recharge elevation ranges from 2865.76 to 4126.69 m. The proportion of cold water mixed in the shallow part is relatively large. A comparative analysis of the genetic models of the three geothermal water groups shows that they share the common feature of being controlled by fault zones. However, they differ in that the Daidao’an geothermal area in Mount Tai is of the karst spring type with a relatively low geothermal water temperature, whereas the Qiaogou geothermal area in Culai Town and the Anjiazhuang geothermal area in Feicheng are of the gravel or sandy shale spring types with a relatively high geothermal water temperature. Full article
(This article belongs to the Topic Human Impact on Groundwater Environment, 2nd Edition)
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24 pages, 3176 KB  
Article
Influence of Seasonality and Pollution on the Presence of Antibiotic Resistance Genes and Potentially Pathogenic Bacteria in a Tropical Urban River
by Kenia Barrantes-Jiménez, Bradd Mendoza-Guido, Eric Morales-Mora, Luis Rivera-Montero, José Montiel-Mora, Luz Chacón-Jiménez, Keilor Rojas-Jiménez and María Arias-Andrés
Antibiotics 2025, 14(8), 798; https://doi.org/10.3390/antibiotics14080798 - 5 Aug 2025
Viewed by 1873
Abstract
Background/Objectives: This study examines how seasonality, pollution, and sample type (water and sediment) influence the presence and distribution of antibiotic resistance genes (ARGs), with a focus on antibiotic resistance genes (ARGs) located on plasmids (the complete set of plasmid-derived sequences, including ARGs) in [...] Read more.
Background/Objectives: This study examines how seasonality, pollution, and sample type (water and sediment) influence the presence and distribution of antibiotic resistance genes (ARGs), with a focus on antibiotic resistance genes (ARGs) located on plasmids (the complete set of plasmid-derived sequences, including ARGs) in a tropical urban river. Methods: Samples were collected from three sites along a pollution gradient in the Virilla River, Costa Rica, during three seasonal campaigns (wet 2021, dry 2022, and wet 2022). ARGs in water and sediment were quantified by qPCR, and metagenomic sequencing was applied to analyze chromosomal and plasmid-associated resistance profiles in sediments. Tobit and linear regression models, along with multivariate ordination, were used to assess spatial and seasonal trends. Results: During the wet season of 2021, the abundance of antibiotic resistance genes (ARGs) such as sul-1, intI-1, and tetA in water samples decreased significantly, likely due to dilution, while intI-1 and tetQ increased in sediments, suggesting particle-bound accumulation. In the wet season 2022, intI-1 remained low in water, qnrS increased, and sediments showed significant increases in tetQ, tetA, and qnrS, along with decreases in sul-1 and sul-2. Metagenomic analysis revealed spatial differences in plasmid-associated ARGs, with the highest abundance at the most polluted site (Site 3). Bacterial taxa also showed spatial differences, with greater plasmidome diversity and a higher representation of potential pathogens in the most contaminated site. Conclusions: Seasonality and pollution gradients jointly shape ARG dynamics in this tropical river. Plasmid-mediated resistance responds rapidly to environmental change and is enriched at polluted sites, while sediments serve as long-term reservoirs. These findings support the use of plasmid-based monitoring for antimicrobial resistance surveillance in aquatic systems. Full article
(This article belongs to the Special Issue Origins and Evolution of Antibiotic Resistance in the Environment)
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27 pages, 18859 KB  
Article
Application of a Hierarchical Approach for Architectural Classification and Stratigraphic Evolution in Braided River Systems, Quaternary Strata, Songliao Basin, NE China
by Zhiwen Dong, Zongbao Liu, Yanjia Wu, Yiyao Zhang, Jiacheng Huang and Zekun Li
Appl. Sci. 2025, 15(15), 8597; https://doi.org/10.3390/app15158597 - 2 Aug 2025
Viewed by 483
Abstract
The description and assessment of braided river architecture are usually limited by the paucity of real geological datasets from field observations; due to the complexity and diversity of rivers, traditional evaluation models are difficult to apply to braided river systems in different climatic [...] Read more.
The description and assessment of braided river architecture are usually limited by the paucity of real geological datasets from field observations; due to the complexity and diversity of rivers, traditional evaluation models are difficult to apply to braided river systems in different climatic and tectonic settings. This study aims to establish an architectural model suitable for the study area setting by introducing a hierarchical analysis approach through well-exposed three-dimensional outcrops along the Second Songhua River. A micro–macro four-level hierarchical framework is adopted to obtain a detailed anatomy of sedimentary outcrops: lithofacies, elements, element associations, and archetypes. Fourteen lithofacies are identified: three conglomerates, seven sandstones, and four mudstones. Five elements provide the basic components of the river system framework: fluvial channel, laterally accreting bar, downstream accreting bar, abandoned channel, and floodplain. Four combinations of adjacent elements are determined: fluvial channel and downstream accreting bar, fluvial channel and laterally accreting bar, erosionally based fluvial channel and laterally accreting bar, and abandoned channel and floodplain. Considering the sedimentary evolution process, the braided river prototype, which is an element-based channel filling unit, is established by documenting three contact combinations between different elements and six types of fine-grained deposits’ preservation positions in the elements. Empirical relationships are developed among the bankfull channel depth, mean bankfull channel depth, and bankfull channel width. For the braided river systems, the establishment of the model promotes understanding of the architecture and evolution, and the application of the hierarchical analysis approach provides a basis for outcrop, underground reservoir, and tank experiments. Full article
(This article belongs to the Section Earth Sciences)
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22 pages, 9790 KB  
Article
Assessing the Hazard of Flooding from Breaching of the Alacranes Dam in Villa Clara, Cuba
by Victor Manuel Carvajal González, Carlos Lázaro Castillo García, Lisdelys González-Rodriguez, Luciana Silva and Jorge Jiménez
Sustainability 2025, 17(15), 6864; https://doi.org/10.3390/su17156864 - 28 Jul 2025
Viewed by 3308
Abstract
Flooding due to dam failures is a critical issue with significant impacts on human safety, infrastructure, and the environment. This study assessed the potential flood hazard that could be generated from breaching of the Alacranes dam in Villa Clara, Cuba. Thirteen reservoir breaching [...] Read more.
Flooding due to dam failures is a critical issue with significant impacts on human safety, infrastructure, and the environment. This study assessed the potential flood hazard that could be generated from breaching of the Alacranes dam in Villa Clara, Cuba. Thirteen reservoir breaching scenarios were simulated under several criteria for modeling the flood wave through the 2D Saint Venant equations using the Hydrologic Engineering Center’s River Analysis System (HEC-RAS). A sensitivity analysis was performed on Manning’s roughness coefficient, demonstrating a low variability of the model outputs for these events. The results show that, for all modeled scenarios, the terrain topography of the coastal plain expands the flood wave, reaching a maximum width of up to 105,057 km. The most critical scenario included a 350 m breach in just 0.67 h. Flood, velocity, and hazard maps were generated, identifying populated areas potentially affected by the flooding events. The reported depths, velocities, and maximum flows could pose extreme danger to infrastructure and populated areas downstream. These types of studies are crucial for both risk assessment and emergency planning in the event of a potential dam breach. Full article
(This article belongs to the Section Hazards and Sustainability)
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36 pages, 25831 KB  
Article
Identification of Cultural Landscapes and Spatial Distribution Characteristics in Traditional Villages of Three Gorges Reservoir Area
by Jia Jiang, Zhiliang Yu and Ende Yang
Buildings 2025, 15(15), 2663; https://doi.org/10.3390/buildings15152663 - 28 Jul 2025
Viewed by 1043
Abstract
The Three Gorges Reservoir Area (TGRA) is an important ecological barrier and cultural intermingling zone in the upper reaches of the Yangtze River, and its traditional villages carry unique information about natural changes and civilisational development, but face the challenges of conservation and [...] Read more.
The Three Gorges Reservoir Area (TGRA) is an important ecological barrier and cultural intermingling zone in the upper reaches of the Yangtze River, and its traditional villages carry unique information about natural changes and civilisational development, but face the challenges of conservation and development under the impact of modernisation and ecological pressure. This study takes 112 traditional villages in the TGRA that have been included in the protection list as the research objects, aiming to construct a cultural landscape identification framework for the traditional villages in the TGRA. Through field surveys, landscape feature assessments, GIS spatial analysis, and multi-source data analysis, we systematically analyse their cultural landscape type systems and spatial differentiation characteristics, and then reveal their cultural landscape types and spatial differentiation patterns. (1) The results of the study show that the spatial distribution of traditional villages exhibits significant altitude gradient differentiation—the low-altitude area is dominated by traffic and trade villages, the middle-altitude area is dominated by patriarchal manor villages and mountain farming villages, and the high-altitude area is dominated by ethno-cultural and ecologically dependent villages. (2) Slope and direction analyses further reveal that the gently sloping areas are conducive to the development of commercial and agricultural settlements, while the steeply sloping areas strengthen the function of ethnic and cultural defence. The results indicate that topographic conditions drive the synergistic evolution of the human–land system in traditional villages through the mechanisms of agricultural optimisation, trade networks, cultural defence, and ecological adaptation. The study provides a paradigm of “nature–humanities” interaction analysis for the conservation and development of traditional villages in mountainous areas, which is of practical value in coordinating the construction of ecological barriers and the revitalisation of villages in the reservoir area. Full article
(This article belongs to the Section Architectural Design, Urban Science, and Real Estate)
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