Search Results (3,847)

Compressed air energy storage in aquifers presents a promising approach for large-scale energy storage, yet its implementation is complicated by geochemical reactions, such as pyrite oxidation, which can impact reservoir integrity and operational efficiency. This study numerically investigates the evolution of reservoir characteristics and geochemical processes during CAESA operations to address these challenges. Using the TOUGHREACT simulator, we developed one-dimensional and two-dimensional reactive transport models based on the Pittsfield aquifer field test parameters to simulate coupled thermal-hydrological–chemical processes under varying injection rates, temperatures, reservoir depths, and operational cycles. The results demonstrate that higher injection rates induce greater near-well pressure buildup and extended thermal zones, while deeper reservoirs exhibit abrupt declines in pressure and gas saturation due to formation constraints. Geochemical analyses reveal that pyrite oxidation dominates, leading to oxygen depletion, groundwater acidification (pH reduction), and secondary mineral precipitation, such as goethite and hematite. These findings underscore the critical interplay between operational parameters and geochemical reactions, highlighting the need for optimized design to ensure long-term stability and efficiency of aquifer-based energy storage systems. Full article

Background: Therapeutic exercises have gained great prominence due to the benefits shown in the treatment of knee osteoarthritis (OA). However, to date, there is no evidence on the effects of an exercise program combined with balance and gait training with visual feedback. Objective: To evaluate the therapeutic effect of an intervention program combining lower-limb muscle strengthening, balance training, and gait exercises with visual feedback on the chronic pain, functional, and biomechanical aspects of older women with and without OA knee. Methods: Clinical trials study with stratified allocation based on disease status (two-arm, triple-blind—assessor, interventionist, and data manager, parallel-group). In total, 40 older women were recruited: 20 in the OA knee group (OAG, n = 20) and 20 in the control group (CG, n = 20). The intervention included a muscular resistance training program in the lower limbs, and reactive and proactive balance and gait training associated with visual feedback. Both groups received the same intervention. The primary outcomes were pain measured by the Visual Analogue Scale and the questionnaires Western Ontario and McMaster Universities Osteoarthritis Index and Lequesne Algofunctional Index. The secondary outcomes were the six-minute walk test, the Falls Risk Awareness Questionnaire, the Timed Up and Go Test, plantar load distribution during gait, and patients’ acceptability. Results: The intervention was effective in improving pain and increasing functionality in older women with OA knee, as measured pre- and post-intervention, compared to the control, with a moderate to high effect size. Body balance increased in older women with OA, as indicated by perceptions of fall risk and walk-test pre- and post-intervention. During gait, a reduction in plantar load (midfoot and rearfoot areas) was observed pre- and post-intervention in OAG compared to the CG. Both groups showed excellent acceptability, suitability, and feasibility of the intervention program. Conclusions: The intervention protocol was effective over 2 consecutive months in reducing pain and increasing knee functionality, balance, walking distance, and perception of falls in older women with OA of the knee compared with women without the condition. During gait, when visual feedback was combined with the intervention protocol, it promoted a better distribution of plantar load over the midfoot and the medial and lateral rearfoot regions in older women with knee OA. Clinical Trial: ReBEC (RBR-5w67pz4). Ethics Committee approval (number: 4.091.004). Full article

Background: Serum albumin is a well-known marker of nutritional and inflammatory status and has been associated with adverse outcomes in heart failure (HF). However, its predictive value for length of hospital-stay and short-term mortality in elderly HF patients remains underexplored. Objectives: To investigate the association between serum albumin levels at hospital admission and length of stay, as well as post-admission mortality, in a cohort of elderly patients hospitalized for HF. Methods: We conducted a retrospective analysis of 56 consecutive patients aged ≥65 years admitted for HF. Comorbidities were assessed using the Cumulative Illness Rating Scale for Geriatrics (CIRS-G), and inflammatory status was measured via C-reactive protein (CRP). Negative binomial regression with robust confidence intervals was employed to evaluate the relationship between serum albumin and length of hospital-stay, adjusting for age, comorbidity burden, and CRP. Cox proportional hazards models were used to assess mortality at 6 months and 1 year, adjusting for age, comorbidity, CRP, and HF subtype, with Kaplan–Meier curves illustrating unadjusted survival differences according to albumin levels and HF subtype. Results: Mean age was 78.6 ± 7.5 years, with 69.6% female patients. Mean serum albumin at admission was 3.58 ± 0.60 g/dL, and mean length of stay was 14.8 ± 10.1 days. Each 1 g/dL increase in albumin was associated with a 32% reduction in length of stay (adjusted IRR = 0.68; 95% CI: 0.54–0.85; p = 0.01), independently by age, inflammatory status and comorbidity. Serum albumin was independently associated with reduced risk of death at 6 months (HR 0.30; 95% CI: 0.11–0.82; p = 0.019) and 1 year (HR = 0.41; 95% CI: 0.17–0.96; p = 0.041). Conclusions: Serum albumin at hospital admission independently predicts length of stay and short-term mortality in elderly patients with HF. Albumin measurement, simple, cheap and universally available biomarker, is helpful for early risk stratification and may guide clinical management in this vulnerable population. Full article

Given the pressing need to strengthen operational reliability in electrical distribution networks, this study proposes an optimization methodology based on the Teaching–Learning-Based Optimization (TLBO) algorithm for the strategic location of redundant lines. The model is validated on the “MV Distribution Network—Base Model” test system, considering the combination of the MTBF (Mean Time Between Failures) and MTTR (Mean Time To Repair) indicators as the objective function. After 500 independent runs, it is determined that the configuration with three redundant lines identified as LN_1011, LN_1058, and LN_0871 offers the most stable solution. Specifically, this topology increases the MTBF from 403.64 h to 409.42 h and reduces the MTTR from 2.351 h to 2.306 h. In addition, significant improvements are observed in the voltage profile and angle, along with a more balanced redistribution of active and reactive power, more efficient use of existing lines, and an overall reduction in energy losses. Full article

Conventional and electronic cigarette (e-cig) users face an increased risk of cardiorespiratory diseases, driven by well-characterized pathways involving inflammation and oxidative stress (OS). Conventional cigarettes contain numerous harmful chemicals, such as nicotine and non-nicotine compounds, which produce reactive oxygen species. Although initially considered a safer alternative, the e-cig still generates toxic aldehydes that are capable of triggering oxidative responses. Heart rate variability (HRV) is an important tool for assessing autonomic function and predicting prognosis. Cardiac autonomic dysfunction, indicated by reduced HRV, has emerged as a critical cardiovascular risk factor associated with several diseases. Clinical and experimental studies show that increased OS is directly associated with heightened sympathetic activity and inversely with parasympathetic modulation. This review demonstrates that exposure to conventional cigarettes smoking and e-cigs adversely affects cardiac autonomic function, detectable by a global reduction in HRV that reflects a shift toward sympathetic dominance and a consequent increase in cardiovascular risk. These changes are associated with increased OS due to nicotine and non-nicotine compounds maintaining sympathovagal imbalance in smokers. Thus, we suggest that autonomic dysfunction, detected by HRV, correlates with oxidative responses and may be used as a modifiable risk factor in longitudinal studies involving both smoking modalities. Full article

Background/Objectives: Apical periodontitis is a prevalent dental condition associated with systemic inflammation. C-reactive protein (CRP) is a sensitive biomarker of inflammatory status. While previous studies have examined CRP changes after endodontic treatment, limited evidence exists on short-term systemic effects following clinically indicated endodontic therapy in healthy individuals. This prospective observational cohort study aimed to evaluate the impact of endodontic treatment or retreatment on serum CRP levels in patients with symptomatic and asymptomatic apical periodontitis and assess demographic influences. No control group was included. Methods: A prospective observational cohort study was conducted in a private endodontic clinic in Jeddah, Saudi Arabia (2021–2023). Three hundred ASA I patients were enrolled and categorized into symptomatic apical periodontitis (SAP) and asymptomatic apical periodontitis (AAP) groups. Blood samples were collected before treatment and two weeks post-treatment. CRP levels were measured using a high-sensitivity assay. Statistical analyses included paired t-tests, Mann–Whitney U tests, and multiple regression. Laboratory personnel were blinded to patient grouping. Results: CRP levels significantly decreased after treatment in both groups (SAP: 6.99 to 2.01 mg/L; AAP: 5.40 to 1.64 mg/L; p < 0.001). Reduction was greater in SAP (mean difference = 4.98 mg/L) than AAP (3.76 mg/L; p < 0.001). Paired t-test showed a very large effect size (Cohen’s d = 3.51). Age and sex were not significant predictors of CRP changes (R² < 0.02). Conclusions: Endodontic treatment or retreatment significantly reduces systemic inflammation in patients with apical periodontitis. These findings reinforce the oral–systemic health link and highlight the clinical relevance of managing apical infections. Longer-term studies and inclusion of additional biomarkers are recommended. Full article

 This paper proposes a novel fractional-order African vulture optimization algorithm (FO-AVOA) for solving the optimal reactive power dispatch (ORPD) problem. By integrating fractional calculus into the conventional AVOA framework, the proposed method enhances the exploration–exploitation balance, accelerates convergence, and improves solution robustness. The ORPD problem is formulated as a constrained optimization task with the objective of minimizing real power losses while satisfying generator voltage limits, transformer tap ratios, and reactive power compensator constraints. The general optimization capability of the FO-AVOA is verified using the CEC 2017, 2020, and 2022 benchmark functions. In addition, the method is applied to the IEEE 30-bus and IEEE 57-bus test systems. The results demonstrate significant power loss reductions of up to 15.888% and 24.39% for the IEEE 30-bus and IEEE 57-bus systems, respectively, compared with the conventional AVOA and other state-of-the-art optimization algorithms, along with strong robustness and stability across independent runs. These findings confirm the effectiveness of the FO-AVOA as a reliable optimization tool for modern power system applications.  Full article

The growing demand for concrete in tropical regions faces two unresolved challenges: the high carbon footprint of ordinary Portland cement (OPC) and limited understanding of how supplementary cementitious materials affect the mechanical performance of laterite rock aggregates concrete. Although metakaolin (MK) is a highly reactive pozzolan, its combined use with laterite rock aggregates concrete and its influence on strength development and microstructure have not been sufficiently clarified. This study investigates the mechanical behavior and sustainability potential of laterite rock aggregate concrete in which OPC is partially replaced by MK at 0%, 5%, 10%, 15%, and 20% by weight. All mixes were prepared at a constant water–binder ratio of 0.50 and tested for workability, compressive strength, split-tensile strength, and flexural strength at 7, 14, and 28 days, with and without a polycarboxylate-based superplasticizer. The results show that MK significantly enhances the mechanical performance of laterite rock concrete, with an optimum at 10% replacement: the 28-day compressive strength increased from 35.6 MPa (control) to 53.9 MPa in the superplasticized mix, accompanied by corresponding gains in tensile and flexural strengths. SEM–EDS analyses revealed microstructural densification, reduced portlandite, and a refined interfacial transition zone, explaining the improved strength and cracking resistance. From an environmental perspective, a 10% MK replacement corresponds to an approximate 10% reduction in clinker-related CO₂ emissions, while the use of locally available laterite rock reduces the dependence on quarried granite and transportation impacts. The findings demonstrate that MK-modified laterite rock concrete is a viable and eco-efficient option for structural applications in tropical regions. The study concludes that MK-enhanced laterite rock aggregate concrete can deliver higher structural performance and improved sustainability without altering conventional mix design and curing practices. Full article

To address trajectory tracking of underactuated unmanned surface vessels (USVs) under disturbances and model uncertainty, we propose a hierarchical control framework that combines model predictive control (MPC) with proximal policy optimization (PPO). The outer loop runs in the inertial reference frame, where an MPC planner based on a kinematic model enforces velocity and safety constraints and generates feasible body–fixed velocity references. The inner loop runs in the body–fixed reference frame, where a PPO policy learns the nonlinear inverse mapping from velocity to multi–thruster thrust, compensating hydrodynamic modeling errors and external disturbances. On top of this framework, we design a Proactive–Reactive Adaptive Reward (PRAR) that uses the MPC prediction sequence and real–time pose errors to adaptively reweight the reward across surge, sway and yaw, improving robustness and cross–model generalization. Simulation studies on circular and curvilinear trajectories compare the proposed PRAR–driven dual–loop controller (PRAR–DLC) with MPC–PID, PPO–Only, MPC–PPO and PPO variants. On the curvilinear trajectory, PRAR–DLC reduces surge MAE and maximum tracking error from 0.269 m and 0.963 m (MPC–PID) to 0.138 m and 0.337 m, respectively; on the circular trajectory it achieves about an 8.5% reduction in surge MAE while maintaining comparable sway and yaw accuracy to the baseline controllers. Real–time profiling further shows that the average MPC and PPO evaluation times remain below the control sampling period, indicating that the proposed architecture is compatible with real–time onboard implementation and physical deployment. Full article

Emerging pollutants such as pharmaceuticals and synthetic dyes increasingly enter agricultural soils through irrigation with treated or untreated wastewater and via biosolid amendments, raising concerns for plant health, soil functionality, and food chain safety. Their environmental behavior is governed by complex interactions between compound physicochemistry, soil properties, and plant physiology, leading to variable persistence, mobility, and ecotoxicological outcomes. This review synthesizes current evidence on the fate, uptake, and phytotoxic effects of drug and dye contaminants in plant–soil systems, and provides a comparative assessment of ecological risks before and after photocatalytic wastewater treatment. The analysis integrates findings from soil- and hydroponic-based studies addressing pollutant sorption–desorption dynamics, leaching, microbial transformations, and plant responses ranging from germination impairment and biomass reduction to oxidative stress and genotoxicity. Special emphasis is given to the formation and behavior of transformation products generated during photocatalytic degradation, which may display altered mobility or toxicity relative to parent compounds. Comparative evaluation reveals that photocatalysis substantially reduces contaminant loads and toxicity in many cases, although incomplete mineralization or the formation of reactive intermediates can sustain or enhance adverse effects under certain conditions. By linking pollutant fate mechanisms with plant and soil responses, this review highlights both the potential and the limitations of photocatalysis as a sustainable strategy for safeguarding agroecosystems in the context of expanding wastewater reuse. Full article

E-cigarettes (E-cigs) are increasing in popularity and are considered a potentially safer alternative to traditional cigarettes. However, prior studies have demonstrated that inhalation of nicotine-containing e-cigs can cause substantial pathophysiologic changes, and “vaping” of some substances has led to severe lung damage. Our group recently described the role of cortactin (CTTN), a cytoskeletal actin-binding regulatory protein, in mediating cigarette smoke (CS) and E-cig-induced lung endothelial apoptosis and mitochondrial dysfunction. In the current study, we advance this work by characterizing the effects of E-cig on lung endothelial nanomechanical properties and barrier function. Lung EC exposure to E-cig extract (50 µg/mL) resulted in disruption of endothelial barrier properties as assessed by Electric Cell–Substrate Impedance Sensing (ECIS). Since excess mitochondrial reactive oxygen species (mitoROS) is an important marker of mitochondrial dysfunction, we next assessed the effect of Mito-TEMPO (10 µM, 3 h), a cell-permeable antioxidant, on E-cig-induced endothelial permeability. Pretreatment with Mito-TEMPO provided EC barrier protection after E-cig challenge, suggesting a key role of mitoROS in E-cig-induced EC permeability. E-cig exposure induces cytoskeleton rearrangement, leading to gap formation in lung EC, and significantly alters EC elastic properties as assessed by atomic force microscopy (AFM). Reduction in CTTN expression by siRNA further augmented the injurious effects of E-cig on EC permeability and elastic properties. This is the first study to explore the role of CTTN in evaluating the effect of E-cigarette exposure on the lung endothelium using AFM and provides novel mitochondrial and biophysical characterization of the effects of E-cig exposure on human lung EC. This work advances our understanding of the pathophysiologic effects of E-cig exposure. Full article

Yeast-derived biomolecules are redefining the boundaries of green nanotechnology. Biosurfactants, exopolysaccharides, enzymes, pigments, proteins, and organic acids—when sourced from carbohydrate-rich lignocellulosic hydrolysates—offer a molecular toolbox capable of directing, stabilizing, and functionalizing nanoparticles (NPs) with unprecedented precision. Beyond their structural diversity and intrinsic biocompatibility, these biomolecules anchor a paradigm shift: the convergence of biorefineries with nanotechnology to deliver multifunctional materials for the circular bioeconomy. This review explores: (i) the expanding portfolio of metallic and metal oxide NPs synthesized through yeast biomolecules; (ii) molecular-level mechanisms of reduction, capping, and surface tailoring that dictate NP morphology, stability, and reactivity; (iii) synergistic roles in intensifying lignocellulosic processes—from enhanced hydrolysis to catalytic upgrading; and (iv) frontier applications spanning antimicrobial coatings, regenerative packaging, precision agriculture, and environmental remediation. We highlight structure–function relationships, where amphiphilicity, charge distribution, and redox activity govern resilience under saline, acidic, and thermally harsh industrial matrices. Yet, critical bottlenecks remain: inconsistent yields, limited comparative studies, downstream recovery hurdles, and the absence of comprehensive life-cycle and toxicological evaluations. To bridge this gap, we propose a translational roadmap coupling standardized characterization with real hydrolysate testing, molecular libraries linking biomolecule chemistry to NP performance, and integrated techno-economic and environmental assessments. By aligning yeast biotechnology with nanoscience, we argue that yeast-biomolecule-driven nanoplatforms are not merely sustainable alternatives but transformative solutions for next-generation lignocellulosic biorefineries. Full article

Colorectal cancer (CRC) incidence is a significant cancer globally, and radiotherapy resistance is a serious problem. Cucurbitacin D (CBD), extracted from many plants such as the tubers of Trichosanthes kirilowii and the fruits of Ecballium elaterium (squirting cucumber), has various therapeutic effects, such as anti-cancer, -inflammation, -diabetes, and -viral infection effects. Since reports have indicated that CBD exhibits effective anti-cancer activity across various cancer types, our hypothesis is that CBD will overcome radioresistance in CRC radiotherapy. In the present study, we identified that CBD, a triterpenoid compound isolated from Trichosanthes kirilowii and Ecballium elaterium, has an anti-cancer and anti-inflammatory effect in vivo and in vitro. In LPS-induced murine models, CBD suppresses LPS-mediated cytokines, including TNFα, IL-6, IL-1β, and COX-2. In CRC xenograft mouse models, CBD treatment results in significantly smaller tumor volumes than the control. In HCT116 and HT29 cells, CBD treatment suppresses cell viability and increases LDH cytotoxicity and caspase-3 activity and cleavage. However, combined treatment of CBD and Z-VAD-FMK inhibits caspase-dependent apoptosis and cell death. Since CBD induces intracellular calcium (Ca²⁺) and reactive oxygen species (ROS) generation, it mediates ER stress-induced apoptotic cell death through the PERK-ATF4-CHOP axis. Moreover, ER stress inducer thapsigargin (TG) mediates synergistic apoptotic cell death in CBD-treated HCT116 and HT29 cells. However, PERK or CHOP knockdown suppresses ER stress-mediated apoptosis in CBD-treated HCT116 and HT29 cells. CBD treatment induces oxidative stress through the NADPH Oxidase 4 (NOX4) and also increases ROS generation. However, NOX4 knockdown and ROS inhibitor NAC or DPI block ER stress-induced apoptotic cell death by inhibiting the suppression of cell viability and the elevation of caspase-3 activity, LDH cytotoxicity, and intracellular ROS activity in CBD-mediated HCT116 and HT29 cells. We established radioresistant CRC models (HCT116R and HT29R); subsequently, radiation (2 Gy) in combination with CBD treatment overcame radioresistance via the modulation of the epithelial–mesenchymal transition (EMT) phenomenon, including the increase in N-cadherin and vimentin and the reduction in E-cadherin. Thus, these results show that CBD may be a new powerful therapeutic approach for CRC radiotherapy. Full article

Background: Acute Respiratory Infections are a common reason for Pediatric Emergency Department (PED) visits. Differentiating bacterial and viral infections may be challenging and might result in incorrect antibiotic prescriptions and exacerbation of antimicrobial resistance. This study evaluated the impact of new diagnostic tests in PED. Methods: A retrospective cohort of 4882 acute febrile respiratory infection cases presenting to the PED was analyzed, comparing two periods: Period 1 (October 2016–March 2017, n = 2181) and Period 2 (October 2023–March 2024, n = 2701). During Period 1, Group A Streptococcus and Respiratory Syncytial Virus rapid antigen detection tests were available. During Period 2, new point-of-care tests (POCTs), including rapid C-reactive protein and rapid antigen detection for Influenza A, Influenza B, and SARS-CoV-2, and a multiplex PCR nasal swab, were introduced. Results: In Period 2, antibiotic prescriptions decreased by 28.4%, along with a reduction in broad-spectrum antibiotic use. A significant correlation was observed between reduced antibiotic prescription and the use of new POCTs and multiplex PCR tests. Performance of blood tests and chest radiographs also decreased. Conclusions: Implementing novel diagnostic tests in PED helps clinicians select more appropriate management options with an impact on reduced stress and radiation exposure and antibiotic prescription. Full article

Failure of the endodontic treatment is often associated with persistent polymicrobial biofilms, particularly those involving Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans), which display synergistic pathogenicity and resistance to standard disinfection methods. This in vitro study compared the antimicrobial activity and oxidative damage induced by indocyanine green (ICG)–mediated laser ablation (LA) with that produced by antimicrobial photodynamic therapy (aPDT) using methylene blue (MB) or curcumin (CUR) in root canals infected with dual-species biofilms. The samples were divided into five experimental groups (n = 20): Group A—Methylene Blue + Red Laser (RL), Group B—Curcumin + Blue LED (BL), Group C—Indocyanine Green + Infrared Diode Laser (DL), Group D—saline solution (Negative Control—NC), Group E—2.5% sodium hypochlorite (Positive Control—PC). One hundred treated bovine incisors (20 per group) were analyzed for microbial viability (colony-forming unit (CFU/mL)), the metabolic functionality of biofilms was assessed through the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) based reduction method, and oxidative stress markers, including Thiobarbituric Acid Reactive Substances (TBARS), protein carbonyl content, total oxidant capacity (TOC), and total protein levels. All experimental treatments significantly reduced microbial load compared to the negative control (p < 0.05), with ICG achieving the greatest reduction. ICG also induced the highest levels of oxidative stress across all parameters (p < 0.05). These findings suggest that LA with ICG is more effective than aPDT with MB or CUR, achieving disinfection outcomes comparable to those of 2.5% sodium hypochlorite, and warrant further investigation in complex clinical models. Full article