Search Results (1,387)

In the context of the global response to climate change and the active promotion of energy transformation, a number of low-carbon policies coupled with the development of synergies to help power system transformation is an important initiative. However, the insufficient articulation of the green power market, tradable green certificate (TGC) market, and carbon emission trading (CET) mechanism, and the ambiguous policy boundaries affect the trading decisions made by its market participants. Therefore, this paper systematically analyses the composition of the main players in the electricity-CET-TGC markets and their relationship with each other, and designs the synergistic mechanism of the electricity-CET-TGC markets, based on which, it constructs the optimal profit model of the thermal power plant operators, renewable energy manufacturers, power grid enterprises, power users and load aggregators under the electricity-CET-TGC markets synergy, and analyses the behavioural decision-making of the main players in the electricity-CET-TGC markets as well as the electric power system to optimise the trading strategy of each player. The results of the study show that: (1) The synergistic mechanism of electricity-CET-TGC markets can increase the proportion of green power grid-connected in the new type of power system. (2) In the selection of different environmental rights and benefits products, the direct participation of green power in the market-oriented trading is the main way, followed by applying for conversion of green power into China certified emission reduction (CCER). (3) The development of independent energy storage technology can produce greater economic and environmental benefits. This study provides policy support to promote the synergistic development of the electricity-CET-TGC markets and assist the low-carbon transformation of the power industry. Full article

Wheelchair-mounted assistive robotic manipulators can provide reach and grasp functions for power wheelchair users. This in-lab study evaluated a vision-guided shared control (VGS) system with twelve users completing two multi-step kitchen tasks: a drinking task and a popcorn making task. Using a mixed methods approach participants compared VGS and manual joystick control, providing performance metrics, qualitative insights, and lessons learned. Data collection included demographic questionnaires, the System Usability Scale (SUS), NASA Task Load Index (NASA-TLX), and exit interviews. No significant SUS differences were found between control modes, but NASA-TLX scores revealed VGS control significantly reduced workload during the drinking task and the popcorn task. VGS control reduced operation time and improved task success but was not universally preferred. Six participants preferred VGS, five preferred manual, and one had no preference. In addition, participants expressed interest in robotic arms for daily tasks and described two main operation challenges: distinguishing wrist orientation from rotation modes and managing depth perception. They also shared perspectives on how a personal robotic arm could complement caregiver support in their home. Full article

The Solar Agro Savior (SAS) is an innovative solution that is assisted by drones for the sustainable utilization of water and plant disease observation in the agriculture sector. This system integrates an alerting mechanism for humidity, moisture, and temperature variations, which affect the plants’ health and optimization in water utilization, which enhances plant yield productivity. A significant feature of the system is the efficient monitoring system in a larger region through drones’ high-resolution cameras, which enables real-time, efficient response and alerting for environmental fluctuations to the authorities. The machine learning algorithm, particularly recurrent neural networks, which is a pioneer with agriculture and pest control, is incorporated for intelligent monitoring systems. The proposed system incorporates a specialized form of a recurrent neural network, Long Short-Term Memory (LSTM), which effectively addresses the vanishing gradient problem. It also utilizes an attention-based mechanism that enables the model to assign meaningful weights to the most important parts of the data sequence. This algorithm not only enhances water utilization efficiency but also boosts plant yield and strengthens pest control mechanisms. This system also provides sustainability through the re-utilization of water and the elimination of electric energy through solar panel systems for powering the inbuilt irrigation system. A comparative analysis of variant algorithms in the agriculture sector with a machine learning approach was also illustrated, and the proposed system yielded 99% yield accuracy, a 97.8% precision value, 98.4% recall, and a 98.4% F1 score value. By encompassing solar irrigation and artificial intelligence-driven analysis, the proposed algorithm, Solar Argo Savior, established a sustainable framework in the latest agricultural sectors and promoted sustainability to protect our environment and community. Full article

Legumes are vital sources of protein, dietary fibre and nutrients, making them crucial for global food security and sustainable agriculture. However, their widespread acceptance and consumption are often limited by undesirable sensory characteristics, such as “a beany flavour”, bitterness or variable textures. Addressing these challenges requires a comprehensive understanding of the complex molecular mechanisms governing appearance, aroma, taste, flavour, texture and palatability in legumes, aiming to enhance their sensory appeal. This review highlights the transformative power of multi-omics approaches in dissecting these intricate biological pathways and facilitating the targeted enhancement of legume sensory qualities. By integrating data from genomics, transcriptomics, proteomics and metabolomics, the genetic and biochemical networks that directly dictate sensory perception can be comprehensively unveiled. The insights gained from these integrated multi-omics studies are proving instrumental in developing strategies for sensory enhancement. They enable the identification of key biomarkers for desirable traits, facilitating more efficient marker-assisted selection (MAS) and genomic selection (GS) in breeding programs. Furthermore, a molecular understanding of sensory pathways opens avenues for precise gene editing (e.g., using CRISPR-Cas9) to modify specific genes, reduce off-flavour compounds or optimise texture. Beyond genetic improvements, multi-omics data also inform the optimisation of post-harvest handling and processing methods (e.g., germination and fermentation) to enhance desirable sensory profiles and mitigate undesirable ones. This holistic approach, spanning from the genetic blueprint to the final sensory experience, will accelerate the development of new legume cultivars and products with enhanced palatability, thereby fostering increased consumption and ultimately contributing to healthier diets and more resilient food systems worldwide. Full article

Surgery remains a healthcare intervention with significant risks for patients. Novel technologies can now enhance the peri-operative workflow, with artificial intelligence (AI) and extended reality (XR) to assist with pre-operative planning. This review focuses on innovation in AI, XR and imaging for hepato-biliary surgery planning. The clinical challenges in hepato-biliary surgery arise from heterogeneity of clinical presentations, the need for multiple imaging modalities and highly variable local anatomy. AI-based models have been developed for risk prediction and multi-disciplinary tumor (MDT) board meetings. The future could involve an on-demand and highly accurate AI-powered decision tool for hepato-biliary surgery, assisting the surgeon to make the most informed decision on the treatment plan, conferring the best possible outcome for individual patients. Advances in AI can also be used to automate image interpretation and 3D modelling, enabling fast and accurate 3D reconstructions of patient anatomy. Surgical navigation systems utilizing XR are already in development, showing an early signal towards improved patient outcomes when used for hepato-biliary surgery. Live visualization of hepato-biliary anatomy in the operating theatre is likely to improve operative safety and performance. The technological advances in AI and XR provide new applications in pre-operative planning with potential for patient benefit. Their use in surgical simulation could accelerate learning curves for surgeons in training. Future research must focus on standardization of AI and XR study reporting, robust databases that are ethically and data protection-compliant, and development of inter-disciplinary tools for various healthcare applications and systems. Full article

Unmanned aerial vehicles (UAVs) have emerged as a promising platform for integrated sensing and communication (ISAC) due to their mobility and deployment flexibility. By adaptively adjusting their flight trajectories, UAVs can maintain favorable line-of-sight (LoS) communication links and sensing angles, thus enhancing overall system performance in dynamic and complex environments. However, ensuring physical layer security (PLS) in such UAV-assisted ISAC systems remains a significant challenge, particularly in the presence of mobile users and potential eavesdroppers. This manuscript proposes a joint optimization framework that simultaneously designs robust transmit beamforming and UAV trajectories to secure downlink communication for multiple ground users. At each time slot, the UAV predicts user positions and maximizes the secrecy sum-rate, subject to constraints on total transmit power, multi-target sensing quality, and UAV mobility. To tackle this non-convex problem, we develop an efficient optimization algorithm based on successive convex approximation (SCA) and constrained optimization by linear approximations (COBYLA). Numerical simulations validate that the proposed framework effectively enhances the secrecy performance while maintaining high-quality sensing, achieving near-optimal performance under realistic system constraints. Full article

Intelligent reflecting surfaces (IRSs) have attracted extensive attention in the design of future communication networks. However, their large number of reflecting elements still results in non-negligible power consumption and hardware costs. To address this issue, we previously proposed a green heterogeneous IRS (HE-IRS) consisting of both dynamically tunable elements (DTEs) and statically tunable elements (STEs). Compared to conventional IRSs with only DTEs, the unique DTE–STE integrated structure introduces new challenges in optimizing the positions and phase shifts of the two types of elements. In this paper, we investigate the element position and phase shift optimization problems in HE-IRS-assisted millimeter-wave systems. We first propose a particle swarm optimization algorithm to determine the specific positions of the DTEs and STEs. Then, by decomposing the phase shift optimization of the two types of elements into two subproblems, we utilize the manifold optimization method to optimize the phase shifts of the STEs, followed by deriving a closed-form solution for those of the DTEs. Furthermore, we propose a low-complexity phase shift optimization algorithm for both DTEs and STEs based on the Cauchy–Schwarz bound. The simulation results show that with the tailored element position and phase shift optimization algorithms, the HE-IRS can achieve a competitive performance compared to that of the conventional IRS, but with much lower power consumption. Full article

Conventional methods for generating knock-out or knock-in mammalian cell models using CRISPR-Cas9 genome editing often require tedious single-cell clone selection and expansion. In this study, we develop and optimise rapid and robust strategies to engineer homozygous fluorescent reporter knock-in cell pools with precise genome editing, circumventing clonal variability inherent to traditional approaches. To reduce false-positive cells associated with random integration, we optimise the design of donor DNA by removing the start codon of the fluorescent reporter and incorporating a self-cleaving T2A peptide system. Using fluorescence-assisted cell sorting (FACS), we efficiently identify and isolate the desired homozygous fluorescent knock-in clones, establishing stable cell pools that preserve parental cell line heterogeneity and faithfully reflect endogenous transcriptional regulation of the target gene. We evaluate the knock-in efficiency and rate of undesired random integration in the electroporation method with either a dual-plasmid system (sgRNA and donor DNA in two separate vectors) or a single-plasmid system (sgRNA and donor DNA combined in one vector). We further demonstrate that coupling our single-plasmid construct with an integrase-deficient lentivirus vector (IDLV) packaging system efficiently generates fluorescent knock-in reporter cell pools, offering flexibility between electroporation and lentivirus transduction methods. Notably, compared to the electroporation methods, the IDLV system significantly minimises random integration. Moreover, the resulting reporter cell lines are compatible with most of the available genome-wide sgRNA libraries, enabling unbiased CRISPR screens to identify key transcriptional regulators of a gene of interest. Overall, our methodologies provide a powerful genetic tool for rapid and robust generation of fluorescent reporter knock-in cell pools with precise genome editing by CRISPR-Cas9 for various research purposes. Full article

Background/Objectives: The demand for accessible and reliable digital health services has increased significantly in recent years, particularly in regions facing physician shortages. HomeDOCtor, a conversational AI platform developed in Slovenia, addresses this need with a nationally adapted architecture that combines retrieval-augmented generation (RAG) and a Redis-based vector database of curated medical guidelines. The objective of this study was to assess the performance and impact of HomeDOCtor in providing AI-powered healthcare assistance. Methods: HomeDOCtor is designed for human-centered communication and clinical relevance, supporting multilingual and multimedia citizen inputs while being available 24/7. It was tested using a set of 100 international clinical vignettes and 150 internal medicine exam questions from the University of Ljubljana to validate its clinical performance. Results: During its six-month nationwide deployment, HomeDOCtor received overwhelmingly positive user feedback with minimal criticism, and exceeded initial expectations, especially in light of widespread media narratives warning about the risks of AI. HomeDOCtor autonomously delivered localized, evidence-based guidance, including self-care instructions and referral suggestions, with average response times under three seconds. On international benchmarks, the system achieved ≥95% Top-1 diagnostic accuracy, comparable to leading medical AI platforms, and significantly outperformed stand-alone ChatGPT-4o in the national context (90.7% vs. 80.7%, p = 0.0135). Conclusions: Practically, HomeDOCtor eases the burden on healthcare professionals by providing citizens with 24/7 autonomous, personalized triage and self-care guidance for less complex medical issues, ensuring that these cases are self-managed efficiently. The system also identifies more serious cases that might otherwise be neglected, directing them to professionals for appropriate care. Theoretically, HomeDOCtor demonstrates that domain-specific, nationally adapted large language models can outperform general-purpose models. Methodologically, it offers a framework for integrating GDPR-compliant AI solutions in healthcare. These findings emphasize the value of localization in conversational AI and telemedicine solutions across diverse national contexts. Full article

Non-orthogonal multiple access (NOMA) technology can effectively improve spectrum efficiency, unmanned aerial vehicle (UAV) communication has the advantage of flexible deployment, and reconfigurable intelligent surface (RIS) can intelligently control the wireless transmission environment. Traditional communication systems have problems such as limited coverage and low spectrum efficiency in complex scenarios. However, a key challenge in deploying RIS-assisted NOMA-UAV communication systems lies in how to jointly optimize the UAV flight trajectory, power allocation strategy, and RIS phase offset to achieve the maximum average achievable rate for users. The non-convex nature of the optimization complicates the problem, making it challenging to find an efficient solution. Based on this, this paper presents a RIS-assisted NOMA-UAV communication system consisting of one UAV, one RIS, and two ground users. To achieve the maximum average rate for users, the UAV flight trajectory, power allocation strategy, and RIS phase offset are jointly optimized. For the non-convex problem, we decompose it into three sub-problems based on its inherent structural characteristics and use an alternating iterative approach to gradually converge to a feasible solution. The simulation results demonstrate that the proposed scheme offers significant advantages in the application scenario. Compared to other benchmark schemes, it delivers superior performance improvements to the communication system and offers higher practical value. Full article

This paper proposes a novel Twin Delayed Deep Deterministic Policy Gradient (TDDPG)-based joint optimization algorithm for hybrid reconfigurable intelligent surface (RIS)-assisted integrated sensing and communication (ISAC) systems in Internet of Vehicles (IoV) scenarios. The proposed system model achieves deep integration of sensing and communication by superimposing the communication and sensing signals within the same waveform. To decouple the complex joint design problem, a dual-DDPG architecture is introduced, in which one agent optimizes the transmit beamforming vector and the other adjusts the RIS phase shift matrix. Both agents share a unified reward function that comprehensively considers multi-user interference (MUI), total transmit power, RIS noise power, and sensing accuracy via the CRLB constraint. Simulation results demonstrate that the proposed TDDPG algorithm significantly outperforms conventional DDPG in terms of sum rate and interference suppression. Moreover, the adoption of a hybrid RIS enables an effective trade-off between communication performance and system energy efficiency, highlighting its practical deployment potential in dynamic IoV environments. Full article

Bacteriophages (phages), the most abundant biological entities on Earth, have long served as both model systems and therapeutic tools. Recent advances in synthetic biology and genetic engineering have revolutionized the capacity to tailor phages with enhanced functionality beyond their natural capabilities. This review outlines the current landscape of synthetic and functional engineering of phages, encompassing both in-vivo and in-vitro strategies. We describe in-vivo approaches such as phage recombineering systems, CRISPR-Cas-assisted editing, and bacterial retron-based methods, as well as synthetic assembly platforms including yeast-based artificial chromosomes, Gibson, Golden Gate, and iPac assemblies. In addition, we explore in-vitro rebooting using TXTL (transcription–translation) systems, which offer a flexible alternative to cell-based rebooting but are less effective for large genomes or structurally complex phages. Special focus is given to the design of customized phages for targeted applications, including host range expansion via receptor-binding protein modifications, delivery of antimicrobial proteins or CRISPR payloads, and the construction of biocontained, non-replicative capsid systems for safe clinical use. Through illustrative examples, we highlight how these technologies enable the transformation of phages into programmable bactericidal agents, precision diagnostic tools, and drug delivery vehicles. Together, these advances establish a powerful foundation for next-generation antimicrobial platforms and synthetic microbiology. Full article

This paper investigated the issues of unstable data collection links and low efficiency in IoT data collection for smart cities by combining active STAR-RIS with UAVs to enhance channel quality, achieving efficient data collection in complex environments. To this end, we propose an active simultaneously transmitting and reflecting reconfigurable intelligent surface (STAR-RIS)-assisted UAV-enabled NOMA data collection system that jointly optimizes active STAR-RIS beamforming, SN power allocation, and UAV trajectory to maximize the system energy efficiency (EE) and the data complete collection rate (DCCR). We apply block coordinate ascent (BCA) to decompose the non-convex problem into three alternating subproblems: combined beamforming optimization of phase shift and amplification gain matrices, power allocation, and trajectory optimization, which are iteratively processed through successive convex approximation (SCA) and fractional programming (FP) methods, respectively. Simulation results demonstrate the proposed algorithm’s rapid convergence and significant advantages over conventional NOMA and OMA schemes in both throughput rate and DCCR. Full article

Respiratory diseases represent a persistent global health challenge, underscoring the need for intelligent, accurate, and personalized diagnostic and therapeutic systems. Existing methods frequently suffer from limitations in diagnostic precision, lack of individualized treatment, and constrained adaptability to complex clinical scenarios. To address these challenges, our study introduces a modular AI-powered framework that integrates an audio-based disease classification model with simulated molecular biomarker profiles to evaluate the feasibility of future multimodal diagnostic extensions, alongside a synthetic-data-driven prescription recommendation engine. The disease classification model analyzes respiratory sound recordings and accurately distinguishes among eight clinical classes: bronchiectasis, pneumonia, upper respiratory tract infection (URTI), lower respiratory tract infection (LRTI), asthma, chronic obstructive pulmonary disease (COPD), bronchiolitis, and healthy respiratory state. The proposed model achieved a classification accuracy of 99.99% on a holdout test set, including 94.2% accuracy on pediatric samples. In parallel, the prescription module provides individualized treatment recommendations comprising drug, dosage, and frequency trained on a carefully constructed synthetic dataset designed to emulate real-world prescribing logic.The model achieved over 99% accuracy in medication prediction tasks, outperforming baseline models such as those discussed in research. Minimal misclassification in the confusion matrix and strong clinician agreement on 200 prescriptions (Cohen’s $\kappa$ = 0.91 [0.87–0.94] for drug selection, 0.78 [0.74–0.81] for dosage, 0.96 [0.93–0.98] for frequency) further affirm the system’s reliability. Adjusted clinician disagreement rates were 2.7% (drug), 6.4% (dosage), and 1.5% (frequency). SHAP analysis identified age and smoking as key predictors, enhancing model explainability. Dosage accuracy was 91.3%, and most disagreements occurred in renal-impaired and pediatric cases. However, our study is presented strictly as a proof-of-concept. The use of synthetic data and the absence of access to real patient records constitute key limitations. A trialed clinical deployment was conducted under a controlled environment with a positive rate of satisfaction from experts and users, but the proposed system must undergo extensive validation with de-identified electronic medical records (EMRs) and regulatory scrutiny before it can be considered for practical application. Nonetheless, the findings offer a promising foundation for the future development of clinically viable AI-assisted respiratory care tools. Full article

The transition towards a power system characterized by a reduced presence of synchronous generators (SGs) and an increased reliance on inverter-based resources (IBRs), including wind, solar photovoltaics (PV), and battery storage, presents new operational challenges, particularly when these sources exceed 50–60% of the system’s demand. While current grid-following (GFL) IBRs, which are equipped with fast and rigid control systems, continue to dominate the inverter landscape, there has been a notable surge in research focused on grid-forming (GFM) inverters in recent years. This study conducts a comparative analysis of the practicality and control methodologies of GFM inverters relative to traditional GFL inverters from a system planning perspective. A comprehensive framework aimed at assisting system developers and consulting engineers in the grid-integration of wide-scale renewable energy sources (RESs), incorporating strategies for the deployment of inverters, capacitor banks, and harmonic filters, is proposed in this paper. The discussion includes an examination of the reactive power capabilities of the plant’s inverters and the provision of additional reactive power to ensure compliance with grid interconnection standards. Furthermore, the paper outlines a practical approach to assess the necessity for enhanced filtering measures to mitigate potential resonant conditions and achieve harmonic compliance at the installation site. The objective of this work is to offer useful guidelines and insights for the effective addition of RES into contemporary power systems. Full article