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17 pages, 4153 KiB  
Article
Spherical Indentation Behavior of DD6 Single-Crystal Nickel-Based Superalloy via Crystal Plasticity Finite Element Simulation
by Xin Hao, Peng Zhang, Hao Xing, Mengchun You, Erqiang Liu, Xuegang Xing, Gesheng Xiao and Yongxi Tian
Materials 2025, 18(15), 3662; https://doi.org/10.3390/ma18153662 - 4 Aug 2025
Abstract
Nickel-based superalloys are widely utilized in critical hot-end components, such as aeroengine turbine blades, owing to their exceptional high-temperature strength, creep resistance, and oxidation resistance. During service, these components are frequently subjected to complex localized loading, leading to non-uniform plastic deformation and microstructure [...] Read more.
Nickel-based superalloys are widely utilized in critical hot-end components, such as aeroengine turbine blades, owing to their exceptional high-temperature strength, creep resistance, and oxidation resistance. During service, these components are frequently subjected to complex localized loading, leading to non-uniform plastic deformation and microstructure evolution within the material. Combining nanoindentation experiments with the crystal plasticity finite element method (CPFEM), this study systematically investigates the effects of loading rate and crystal orientation on the elastoplastic deformation of DD6 alloy under spherical indenter loading. The results indicate that the maximum indentation depth increases and hardness decreases with prolonged loading time, exhibiting a significant strain rate strengthening effect. The CPFEM model incorporating dislocation density effectively simulates the nonlinear characteristics of the nanoindentation process and elucidates the evolution of dislocation density and slip system strength with indentation depth. At low loading rates, both dislocation density and slip system strength increase with loading time. Significant differences in mechanical behavior are observed across different crystal orientations, which correspond to the extent of lattice rotation during texture evolution. For the [111] orientation, crystal rotation is concentrated and highly regular, while the [001] orientation shows uniform texture evolution. This demonstrates that anisotropy governs the deformation mechanism through differential slip system activation and texture evolution. Full article
(This article belongs to the Special Issue Nanoindentation in Materials: Fundamentals and Applications)
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10 pages, 2384 KiB  
Article
Effects of Grain Size on Mechanical Properties of Nanopolycrystalline Fe-Al Alloy
by Xiaoming Liu, Kun Gao, Long Huang, Peng Chen and Jing Yang
Processes 2025, 13(8), 2462; https://doi.org/10.3390/pr13082462 - 4 Aug 2025
Viewed by 11
Abstract
FeAl intermetallic compounds exhibit high application potential in high-voltage transmission lines to withstand external forces such as powerlines’ own gravity and wind force. The ordered crystal structure in FeAl intermetallic compounds endows materials with high strength, but the remarkable brittleness at room temperature [...] Read more.
FeAl intermetallic compounds exhibit high application potential in high-voltage transmission lines to withstand external forces such as powerlines’ own gravity and wind force. The ordered crystal structure in FeAl intermetallic compounds endows materials with high strength, but the remarkable brittleness at room temperature restricts engineering applications. This contradiction is essentially closely related to the deformation mechanism at the nanoscale. Here, we performed molecular dynamics simulations to reveal anomalous grain size effects and deformation mechanisms in nanocrystalline FeAl intermetallic material. Models with grain sizes ranging from 6.2 to 17.4 nm were systematically investigated under uniaxial tensile stress. The study uncovers a distinctive inverse Hall-Petch relationship governing flow stress within the nanoscale regime. This behavior stems from high-density grain boundaries promoting dislocation annihilation over pile-up. Crucially, the material exhibits anomalous ductility at ultra-high strain rates due to stress-induced phase transformation dominating the plastic deformation. The nascent FCC phase accommodates strain through enhanced slip systems and inherent low stacking fault energy with the increasing phase fraction paralleling the stress plateau. Nanoconfinement suppresses the propagation of macroscopic defects while simultaneously suppressing room-temperature brittle fracture and inhibiting the rapid phase transformation pathways at extreme strain rates. These findings provide new theoretical foundations for designing high-strength and high-toughness intermetallic nanocompounds. Full article
(This article belongs to the Section Materials Processes)
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16 pages, 1526 KiB  
Article
Effects of Different Phosphorus Addition Levels on Physiological and Growth Traits of Pinus massoniana (Masson Pine) Seedlings
by Zhenya Yang and Hui Wang
Forests 2025, 16(8), 1265; https://doi.org/10.3390/f16081265 - 2 Aug 2025
Viewed by 140
Abstract
Soil phosphorus (P) availability is an important determinant of productivity in Pinus massoniana (Masson pine) forests. The mechanistic bases governing the physiological and growth responses of Masson pine to varying soil P conditions remain insufficiently characterized. This study aims to decipher the adaptive [...] Read more.
Soil phosphorus (P) availability is an important determinant of productivity in Pinus massoniana (Masson pine) forests. The mechanistic bases governing the physiological and growth responses of Masson pine to varying soil P conditions remain insufficiently characterized. This study aims to decipher the adaptive strategies of Masson pine to different soil P levels, focusing on root morphological–architectural plasticity and the allocation dynamics of nutrient elements and photosynthetic assimilates. One-year-old potted Masson pine seedlings were exposed to four P addition treatments for one year: P0 (0 mg kg−1), P1 (25 mg kg−1), P2 (50 mg·kg−1), and P3 (100 mg kg−1). In July and December, measurements were conducted on seedling organ biomass, root morphological indices [root length (RL), root surface area (RSA), root diameter (RD), specific root length (SRL), and root length ratio (RLR) for each diameter grade], root architectural indices [number of root tips (RTs), fractal dimension (FD), root branching angle (RBA), and root topological index (TI)], as well as the content of nitrogen (N), phosphorus (P), carbon (C), and non-structural carbohydrates (NSCs) in roots, stems, and leaves. Compared with the P0 treatment, P2 and P3 significantly increased root biomass, root–shoot ratio, RL, RSA, RTs, RLR of finer roots (diameter ≤ 0.4 mm), nutrient accumulation ratio in roots, and starch (ST) content in roots, stems and leaves. Meanwhile, they decreased soluble sugar (SS) content, SS/ST ratio, C and N content, and N/P and C/P ratios in stems and leaves, as well as nutrient accumulation ratio in leaves. The P3 treatment significantly reduced RBA and increased FD and SRL. Our results indicated that Masson pine adapts to low P by developing shallower roots with a reduced branching intensity and promoting the conversion of ST to SS. P’s addition effectively alleviates growth limitations imposed by low P, stimulating root growth, branching, and gravitropism. Although a sole P addition promotes short-term growth and P uptake, it triggers a substantial consumption of N, C, and SS, leading to significant decreases in N/P and C/P ratios and exacerbating N’s limitation, which is detrimental to long-term growth. Under high-P conditions, Masson pine strategically prioritizes allocating limited N and SS to roots, facilitating the formation of thinner roots with low C costs. Full article
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14 pages, 5622 KiB  
Article
Molecular Dynamics Simulations on the Deformation Behaviors and Mechanical Properties of the γ/γ′ Superalloy with Different Phase Volume Fractions
by Xinmao Qin, Wanjun Yan, Yilong Liang and Fei Li
Crystals 2025, 15(8), 706; https://doi.org/10.3390/cryst15080706 - 31 Jul 2025
Viewed by 161
Abstract
Based on molecular dynamics simulation, we conducted a comprehensive study on the tensile behaviors and properties of the γ(Ni)/γ(Ni3Al) superalloy with varying γ(Ni3Al) phase volume fractions (Vγ) under high-temperature, [...] Read more.
Based on molecular dynamics simulation, we conducted a comprehensive study on the tensile behaviors and properties of the γ(Ni)/γ(Ni3Al) superalloy with varying γ(Ni3Al) phase volume fractions (Vγ) under high-temperature, high-strain-rate service environments. Our investigation revealed that the tensile behavior of the superalloy depends critically on the Vγ. When the Vγ increased from 13.5 to 67%, the system’s tensile strength exhibited a non-monotonic response, peaking at Vγ = 40.3% before progressively decreasing. Conversely, the maximum uniform plastic strain decreased linearly and significantly when Vγ increased. These results establish an atomistically informed framework that elucidates the composition–microstructure–property relationships in γ(Ni)/γ(Ni3Al) superalloys, specifically addressing how Vγ governs variations in deformation mechanisms and mechanical performance. Furthermore, this work provides quantitative design paradigm for optimizing γ(Ni3Al) precipitate architecture and compositional tuning in the Ni-based γ(Ni)/γ(Ni3Al) superalloy. Full article
(This article belongs to the Special Issue Advances in High-Performance Alloys)
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4 pages, 269 KiB  
Editorial
Beyond a Simple Switch: Decoding the Multifactorial Phenotypic Plasticity of Vascular Smooth Muscle Cells
by Francisca Muñoz, Claire M. Holden and Alejandra San Martin
Cells 2025, 14(15), 1171; https://doi.org/10.3390/cells14151171 - 30 Jul 2025
Viewed by 227
Abstract
Vascular smooth muscle cells (VSMCs) are central to the maintenance of vascular homeostasis and the progression of cardiovascular diseases (CVDs), owing to their remarkable phenotypic plasticity. This editorial introduces a Special Issue of Cells that compiles recent advances in our understanding of the [...] Read more.
Vascular smooth muscle cells (VSMCs) are central to the maintenance of vascular homeostasis and the progression of cardiovascular diseases (CVDs), owing to their remarkable phenotypic plasticity. This editorial introduces a Special Issue of Cells that compiles recent advances in our understanding of the molecular, epigenetic, metabolic, and mechanical mechanisms that govern VSMC behavior. Highlighted contributions explore the roles of RNA modifications, chromatin remodeling, lipid metabolism, and mechanotransduction in VSMC phenotypic switching, revealing new therapeutic targets and diagnostic opportunities. Together, these studies emphasize the multifactorial regulation of VSMC plasticity and its dual role in vascular repair and disease pathogenesis. Full article
(This article belongs to the Special Issue Role of Vascular Smooth Muscle Cells in Cardiovascular Disease)
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15 pages, 9051 KiB  
Article
Mechanical Properties and Fatigue Life Estimation of Selective-Laser-Manufactured Ti6Al4V Alloys in a Comparison Between Annealing Treatment and Hot Isostatic Pressing
by Xiangxi Gao, Xubin Ye, Yuhuai He, Siqi Ma and Pengpeng Liu
Materials 2025, 18(15), 3475; https://doi.org/10.3390/ma18153475 - 24 Jul 2025
Viewed by 167
Abstract
Selective laser melting (SLM) offers a novel approach for manufacturing intricate structures, broadening the application of titanium alloy parts in the aerospace industry. After the build period, heat treatments of annealing (AT) and hot isostatic pressing (HIP) are often implemented, but a comparison [...] Read more.
Selective laser melting (SLM) offers a novel approach for manufacturing intricate structures, broadening the application of titanium alloy parts in the aerospace industry. After the build period, heat treatments of annealing (AT) and hot isostatic pressing (HIP) are often implemented, but a comparison of their mechanical performances based on the specimen orientation is still lacking. In this study, horizontally and vertically built Ti6Al4V SLM specimens that underwent the aforementioned treatments, together with their microstructural and defect characteristics, were, respectively, investigated using metallography and X-ray imaging. The mechanical properties and failure mechanism, via fracture analysis, were obtained. The critical factors influencing the mechanical properties and the correlation of the fatigue lives and failure origins were also estimated. The results demonstrate that the mechanical performances were determined by the α-phase morphology and defects, which included micropores and fewer large lack-of-fusion defects. Following the coarsening of the α phase, the strength decreased while the plasticity remained stable. With the discrepancy in the defect occurrence, anisotropy and scatter of the mechanical performances were introduced, which was significantly alleviated with HIP treatment. The fatigue failure origins were governed by defects and the α colony, which was composed of parallel α phases. Approximately linear relationships correlating fatigue lives with the X-parameter and maximum stress amplitude were, respectively, established in the AT and HIP states. The results provide an understanding of the technological significance of the evaluation of mechanical properties. Full article
(This article belongs to the Section Metals and Alloys)
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29 pages, 7048 KiB  
Article
Research on Synergistic Control Technology for Composite Roofs in Mining Roadways
by Lei Wang, Gang Liu, Dali Lin, Yue Song and Yongtao Zhu
Processes 2025, 13(8), 2342; https://doi.org/10.3390/pr13082342 - 23 Jul 2025
Viewed by 202
Abstract
Addressing the stability control challenges of roadways with composite roofs in the No. 34 coal seam of Donghai Mine under high-strength mining conditions, this study employed integrated methodologies including laboratory experiments, numerical modeling, and field trials. It investigated the mechanical response characteristics of [...] Read more.
Addressing the stability control challenges of roadways with composite roofs in the No. 34 coal seam of Donghai Mine under high-strength mining conditions, this study employed integrated methodologies including laboratory experiments, numerical modeling, and field trials. It investigated the mechanical response characteristics of the composite roof and developed a synergistic control system, validated through industrial application. Key findings indicate significant differences in mechanical behavior and failure mechanisms between individual rock specimens and composite rock masses. A theoretical “elastic-plastic-fractured” zoning model for the composite roof was established based on the theory of surrounding rock deterioration, elucidating the mechanical mechanism where the cohesive strength of hard rock governs the load-bearing capacity of the outer shell, while the cohesive strength of soft rock controls plastic flow. The influence of in situ stress and support resistance on the evolution of the surrounding rock zone radii was quantitatively determined. The FLAC3D strain-softening model accurately simulated the post-peak behavior of the surrounding rock. Analysis demonstrated specific inherent patterns in the magnitude, ratio, and orientation of principal stresses within the composite roof under mining influence. A high differential stress zone (σ1/σ3 = 6–7) formed within 20 m of the working face, accompanied by a deflection of the maximum principal stress direction by 53, triggering the expansion of a butterfly-shaped plastic zone. Based on these insights, we proposed and implemented a synergistic control system integrating high-pressure grouting, pre-stressed cables, and energy-absorbing bolts. Field tests demonstrated significant improvements: roof-to-floor convergence reduced by 48.4%, rib-to-rib convergence decreased by 39.3%, microseismic events declined by 61%, and the self-stabilization period of the surrounding rock shortened by 11%. Consequently, this research establishes a holistic “theoretical modeling-evolution diagnosis-synergistic control” solution chain, providing a validated theoretical foundation and engineering paradigm for composite roof support design. Full article
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26 pages, 1698 KiB  
Review
Research Progress on the Functional Regulation Mechanisms of ZKSCAN3
by Jianxiong Xu, Xinzhe Li, Jingjing Xia, Wenfang Li and Zhengding Su
Biomolecules 2025, 15(7), 1016; https://doi.org/10.3390/biom15071016 - 14 Jul 2025
Viewed by 492
Abstract
The zinc finger protein with KRAB and SCAN domains 3 (ZKSCAN3) has emerged as a critical regulator of diverse cellular processes, including autophagy, cell cycle progression, and tumorigenesis. Structurally, ZKSCAN3 is characterized by its conserved DNA-binding zinc finger motifs, a SCAN domain mediating [...] Read more.
The zinc finger protein with KRAB and SCAN domains 3 (ZKSCAN3) has emerged as a critical regulator of diverse cellular processes, including autophagy, cell cycle progression, and tumorigenesis. Structurally, ZKSCAN3 is characterized by its conserved DNA-binding zinc finger motifs, a SCAN domain mediating protein–protein interaction, and a KRAB repression domain implicated in transcriptional regulation. Post-translational modifications, such as phosphorylation and ubiquitination, dynamically modulate its subcellular localization and activity, enabling context-dependent functional plasticity. Functionally, ZKSCAN3 acts as a master switch in autophagy by repressing the transcription of autophagy-related genes under nutrient-replete conditions, while its nuclear-cytoplasmic shuttling under stress conditions links metabolic reprogramming to cellular survival. Emerging evidence also underscores its paradoxical roles in cancer: it suppresses tumor initiation by maintaining genomic stability yet promotes metastasis through epithelial–mesenchymal transition induction. Furthermore, epigenetic mechanisms, including promoter methylation and non-coding RNA regulation, fine-tune ZKSCAN3 expression, contributing to tissue-specific outcomes. Despite these insights, gaps remain in understanding the structural determinants governing its interaction with chromatin-remodeling complexes and the therapeutic potential of targeting ZKSCAN3 in diseases. Future investigations should prioritize integrating multi-omics approaches to unravel context-specific regulatory networks and explore small-molecule modulators for translational applications. This comprehensive analysis provides a framework for advancing our mechanistic understanding of ZKSCAN3 and its implications in human health and disease. This review synthesizes recent advances in elucidating the regulatory networks and functional complexity of ZKSCAN3, highlighting its dual roles in physiological and pathological contexts. Full article
(This article belongs to the Special Issue Spotlight on Hot Cancer Biological Biomarkers)
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28 pages, 17257 KiB  
Article
A Crystal Plasticity Phase-Field Study on the Effects of Grain Boundary Degradation on the Fatigue Behavior of a Nickel-Based Superalloy
by Pengfei Liu, Zhanghua Chen, Xiao Zhao, Jianxin Dong and He Jiang
Materials 2025, 18(14), 3309; https://doi.org/10.3390/ma18143309 - 14 Jul 2025
Viewed by 374
Abstract
Grain boundary weakening in high-temperature environments significantly influences the fatigue crack growth mechanisms of nickel-based superalloys, introducing challenges in accurately predicting fatigue life. In this study, a dislocation-density-based crystal plasticity phase-field (CP–PF) model is developed to simulate the fatigue crack growth behavior of [...] Read more.
Grain boundary weakening in high-temperature environments significantly influences the fatigue crack growth mechanisms of nickel-based superalloys, introducing challenges in accurately predicting fatigue life. In this study, a dislocation-density-based crystal plasticity phase-field (CP–PF) model is developed to simulate the fatigue crack growth behavior of the GH4169 alloy under both room and elevated temperatures. Grain boundaries are explicitly modeled, enabling the competition between transgranular and intergranular cracking to be accurately captured. The grain boundary separation energy and surface energy, calculated via molecular dynamics simulations, are employed as failure criteria for grain boundary and intragranular material points, respectively. The simulation results reveal that under oxygen-free conditions, fatigue crack propagation at both room and high temperatures is governed by sustained shear slip, with crack advancement hindered by grains exhibiting low Schmid factors. When grain boundary oxidation is introduced, increasing oxidation levels progressively degrade grain boundary strength and reduce overall fatigue resistance. Specifically, at room temperature, oxidation shortens the duration of crack arrest near grain boundaries. At elevated service temperatures, intensified grain boundary degradation facilitates a transition in crack growth mode from transgranular to intergranular, thereby accelerating crack propagation and exacerbating fatigue damage. Full article
(This article belongs to the Section Metals and Alloys)
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19 pages, 1302 KiB  
Article
Low-Carbon, Low-Shrinkage Concrete Design Based on Paste–Aggregate Binary Model
by Chunming Lian, Xiong Zhang, Lu Han, Weijun Wen, Wenbiao Lin and Lifang Han
Materials 2025, 18(14), 3292; https://doi.org/10.3390/ma18143292 - 12 Jul 2025
Viewed by 368
Abstract
This study presents a performance-based concrete mix design methodology rooted in the paste–aggregate binary framework, aiming to reduce binder content while ensuring optimal workability and strength. We found that inter-particle spacing (SPT) and paste rheology jointly govern fresh concrete behavior, with slump increasing [...] Read more.
This study presents a performance-based concrete mix design methodology rooted in the paste–aggregate binary framework, aiming to reduce binder content while ensuring optimal workability and strength. We found that inter-particle spacing (SPT) and paste rheology jointly govern fresh concrete behavior, with slump increasing nonlinearly with SPT and a critical transition zone around 20–35 µm; paste yield stress controls slump, while plastic viscosity governs segregation resistance. A two-level strength model was developed to predict concrete strength from paste properties based on compactness and hydration (R2 = 0.90). Fixing SPT at 25 µm was identified as optimal for achieving balanced flowability with minimal paste volume. This approach effectively decouples aggregate packing optimization from paste calibration, offering a physically interpretable and practical framework for designing sustainable, low-carbon, and low-shrinkage concrete. Full article
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20 pages, 10945 KiB  
Article
Temperature-Dependent Deformation Mechanisms in Ti65 Alloy: An In Situ Tensile Study
by Haitao Li, Chenxu Li, Dongmei Chen, Yujing Liu, Zibo Zhao, Bohua Zhang, Meng Qi, Jianrong Liu and Qingjiang Wang
Materials 2025, 18(14), 3270; https://doi.org/10.3390/ma18143270 - 11 Jul 2025
Viewed by 336
Abstract
Understanding the relationship between deformation behavior and mechanisms at elevated temperatures is of great significance for applications of high-temperature titanium alloys. This study systematically investigates the plastic deformation behavior of Ti65 alloy under both room-temperature and high-temperature conditions through in situ tensile testing, [...] Read more.
Understanding the relationship between deformation behavior and mechanisms at elevated temperatures is of great significance for applications of high-temperature titanium alloys. This study systematically investigates the plastic deformation behavior of Ti65 alloy under both room-temperature and high-temperature conditions through in situ tensile testing, combined with slip trace analysis, crystal orientation analysis, and geometrical compatibility factor evaluation. TEM observations and molecular dynamics simulations reveal that plastic deformation is predominantly accommodated by basal and prismatic slip systems with minimal pyramidal slip contribution at room temperature. However, elevated temperatures significantly promote pyramidal <a> and <c+a> slip due to thermal activation. This transition stems from a shift in deformation mechanisms: while room-temperature deformation relies on multi-slip and grain rotation to accommodate strain, high-temperature deformation is governed by efficient slip transfer across grain boundaries enabled by enhanced geometrical compatibility. Consistent with this, thermal activation at elevated temperatures reduces the critical resolved shear stress (CRSS), preferentially activating 1/3<11–23> dislocations and thereby substantially improving plastic deformation capability. These findings provide critical insights into the temperature-dependent deformation mechanisms of Ti65 alloy, offering valuable guidance for performance optimization in high-temperature applications. Full article
(This article belongs to the Section Metals and Alloys)
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24 pages, 2179 KiB  
Article
Time-Dependent Rheological Behavior and MPS Simulation of Cement–Bentonite Slurries with Hydration Accelerators for Borehole Backfilling Applications
by Shinya Inazumi, Kazuhiko Tazuke and Seiya Kashima
J. Compos. Sci. 2025, 9(7), 361; https://doi.org/10.3390/jcs9070361 - 10 Jul 2025
Viewed by 578
Abstract
This study investigates cement–bentonite slurries with hydration accelerators for borehole backfilling applications in infrastructure reconstruction projects. Two formulations with different accelerator dosages (5 and 10 kg/m3) were evaluated through combined experimental testing and Moving Particle Semi-implicit (MPS) numerical modeling to optimize [...] Read more.
This study investigates cement–bentonite slurries with hydration accelerators for borehole backfilling applications in infrastructure reconstruction projects. Two formulations with different accelerator dosages (5 and 10 kg/m3) were evaluated through combined experimental testing and Moving Particle Semi-implicit (MPS) numerical modeling to optimize material performance. The research focuses on time-dependent rheological evolution and its impact on construction performance, particularly bleeding resistance and workability retention. Experimental flow tests revealed that both formulations maintained similar initial flowability (240–245 mm spread diameter), but the higher accelerator dosage resulted in 33% flow reduction after 60 min compared to 12% for the lower dosage. Bleeding tests demonstrated significant improvement in phase stability, with bleeding rates reduced from 2.5% to 1.5% when accelerator content was doubled. The MPS framework successfully reproduced experimental behavior with prediction accuracies within 3%, enabling quantitative analysis of time-dependent rheological parameters through inverse analysis. The study revealed that yield stress evolution governs both flow characteristics and bleeding resistance, with increases several hundred percent over 60 min while plastic viscosity remained relatively constant. Critically, simulations incorporating time-dependent viscosity changes accurately predicted bleeding behavior, while constant-viscosity models overestimated bleeding rates by 60–130%. The higher accelerator formulation (10 kg/m3) provided an optimal balance between initial workability and long-term stability for typical borehole backfilling operations. This integrated experimental–numerical approach provides practical insights for material optimization in infrastructure reconstruction projects, particularly relevant for aging infrastructure requiring proper foundation treatment. The methodology offers construction practitioners a robust framework for material selection and performance prediction in borehole backfilling applications, contributing to improved construction quality and reduced project risks. Full article
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24 pages, 24243 KiB  
Article
Seismic Damage Mechanism of Five-Story and Three-Span Underground Complex in Soft Soil Site
by Yikun Liu, Qingjun Chen, Xi Chen and Cong Liao
Buildings 2025, 15(14), 2380; https://doi.org/10.3390/buildings15142380 - 8 Jul 2025
Viewed by 313
Abstract
Investigating the seismic damage mechanism of large underground complexes is essential for the safe development of urban underground space. This paper examines a five-story and three-span underground complex situated in a soft soil site. Shaking table tests were designed and conducted on both [...] Read more.
Investigating the seismic damage mechanism of large underground complexes is essential for the safe development of urban underground space. This paper examines a five-story and three-span underground complex situated in a soft soil site. Shaking table tests were designed and conducted on both the free field and the soil–underground complex interaction system. The time–frequency evolution of the free field under various seismic motions was investigated. A combined experimental and numerical simulation approach was employed to examine the seismic response of the soil–underground complex interaction system. The structural deformation evolution, stress distribution, and development process of plastic damage under different seismic motions were analyzed. The results reveal that soft soil exhibits a significant energy amplification effect under far-field long-period ground motions. Structural deformation is mainly governed by horizontal shear. Under strong seismic excitation, plastic damage first initiates at the end of the bottom-story columns and extends to column-to-slab and wall-to-slab connections, where abrupt stiffness changes occur. Under the far-field long-period ground motion, the structural deformation, stress distribution, and plastic damage are significantly greater than those under the Shanghai artificial wave. These findings provide valuable insights for the seismic design of large underground complexes in soft soil sites. Full article
(This article belongs to the Section Building Structures)
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18 pages, 6872 KiB  
Article
Evolutionary Dynamics and Functional Conservation of amh Signaling in Teleost Lineages
by Lingqun Zhang, Qingke Zhang, Kai Hu, Wei Lu, Weigang Li, Fengchi Wang and Jie Cheng
Fishes 2025, 10(7), 327; https://doi.org/10.3390/fishes10070327 - 3 Jul 2025
Viewed by 325
Abstract
The anti-Müllerian hormone (amh) and its receptor, amhr2, along with the downstream bone morphogenetic protein receptors (bmprs), have been recognized as the central regulators in teleost sex determination (SD) and differentiation. However, their evolution and function in reproduction [...] Read more.
The anti-Müllerian hormone (amh) and its receptor, amhr2, along with the downstream bone morphogenetic protein receptors (bmprs), have been recognized as the central regulators in teleost sex determination (SD) and differentiation. However, their evolution and function in reproduction among diverse teleost lineages may represent species-specific patterns and still need more explanation. In this study, systematic investigations of amh signaling genes, including amh, amhy (Y-linked paralog of amh), amhr2, bmpr1, and bmpr2, were conducted among teleost species. The results revealed generally conserved gene copy number, phylogeny, structure, and synteny, among teleost amh signaling genes. Notably, significantly accelerated evolutionary rates (dN/dS) were found in teleost amhy compared to amh, and amh exhibited faster molecular evolution in amhy-SD teleosts than in non-amhy-SD teleosts, suggesting their enhanced evolutionary plasticity in teleosts. Expression profiling identified testis-biased expression of the most amh signaling genes in fish species with different SD genes and mechanisms, including Lateolabrax maculatus and Dicentrarchus labrax from Order Perciformes, Cynoglossus semilaevis and Paralichthys olivaceus from Order Pleuronectiformes, and Salmo salar and Oncorhynchus mykiss from Order Salmoniformes, with ovary-biased expression also found in Salmoniformes. A weighted gene co-expression network analysis further uncovered strong species-specific functional interactions between amh signaling components and genes of germ-cell development, the meiotic process, etc. Collectively, the integrated evidence from this study supports the hypothesis that amh signaling provides the key molecules governing sex differentiation in a species-specific manner in diverse teleost lineages, independent of its SD role, and interacts with functions of both testis and ovary development. Full article
(This article belongs to the Special Issue Sex and Reproductive Regulation in Marine Animals)
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16 pages, 6077 KiB  
Review
Evolutionary and Structural Analysis of the Aquaporin Gene Family in Rice
by Tao Tong, Fanrong Zeng, Shuzhen Ye, Zhijuan Ji, Yanli Wang, Zhong-Hua Chen and Younan Ouyang
Plants 2025, 14(13), 2035; https://doi.org/10.3390/plants14132035 - 3 Jul 2025
Viewed by 504
Abstract
Aquaporins in rice (Oryza sativa L.) represent a pivotal class of transmembrane channel proteins that mediate the bidirectional transport of water and small solutes, which have critical functions in cellular osmoregulation and ion homeostasis maintenance. Their evolutionary diversity and functional plasticity constitute [...] Read more.
Aquaporins in rice (Oryza sativa L.) represent a pivotal class of transmembrane channel proteins that mediate the bidirectional transport of water and small solutes, which have critical functions in cellular osmoregulation and ion homeostasis maintenance. Their evolutionary diversity and functional plasticity constitute fundamental mechanisms underlying the adaptive responses to diversified environmental challenges. This review systematically summarizes rice AQPs’ evolutionary origins, structural characteristics, and spatiotemporal expression patterns under both physiological and stress conditions, highlighting the high conservation of their key functional domains across evolution and their environment-driven functional diversification. The molecular mechanisms governing AQPs in water utilization, nutrient uptake, and stress responses are unraveled. Furthermore, the potential of precision gene editing and multi-omics integration is discussed to decipher the intricate relationships between AQP evolutionary history, environmental adaptability, and functional specialization, thereby providing a theoretical basis for advancing crop stress resistance and high-quality breeding. Full article
(This article belongs to the Special Issue Cell Physiology and Stress Adaptation of Crops)
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