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Keywords = plant-derived dye

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24 pages, 22474 KB  
Article
Coreopsis tinctoria Nutt. As a New Raw Material for Natural Hair Coloration: Discovering the Dyeing Potential of Chalcones
by Jule Marie Sauler, Volkmar Vill and Fabian Straske
Cosmetics 2026, 13(3), 127; https://doi.org/10.3390/cosmetics13030127 - 20 May 2026
Viewed by 115
Abstract
This study presents the development of a natural hair dye based on Coreopsis tinctoria Nutt. plant extract with and without subsequent mordanting. The dye molecules behind the color development have been investigated to gain better understanding of the relationship between flavonoid structure and [...] Read more.
This study presents the development of a natural hair dye based on Coreopsis tinctoria Nutt. plant extract with and without subsequent mordanting. The dye molecules behind the color development have been investigated to gain better understanding of the relationship between flavonoid structure and color on hair. Yak hair was dyed under different conditions and wash fastness tests were carried out to evaluate the performance of the new hair dye. Analysis of Coreopsis tinctoria Nutt. plant extract was performed to assess the chemical constitution of hair dye. Coreopsis tinctoria Nutt. extract solely led to yellow colors (as represented within L*a*b* color space: L* = 65.5; a* = 0.7; b* = 46.6), whereas the treatment combined with ferrous lactate led to dark brown colors (L* = 26.4; a* = 2.3; b* = 10.0). Wash fastness demonstrated a very good color stability with a maximum loss in color intensity of ΔE = 12.4 after 24 hair washes. Dyeing experiments using the most abundant flavonoids marein, flavanomarein, okanin, and isookanin gave insight into the responsible flavonoids for color outcome. In combination with ferrous lactate, chalcones led to brown colors and flavanones to gray colors. The chalcone okanin presented itself as the most powerful dye, leading to intense colors in combination with ferrous lactate (ΔE = 56.6), at low dye concentrations of 0.1 mg mL−1. Full article
(This article belongs to the Special Issue Feature Papers in Cosmetics in 2026)
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31 pages, 3692 KB  
Review
Application of Plant Polyphenols in Multifunctional Textiles
by Xi Liang and Yue-Rong Liang
Textiles 2026, 6(2), 53; https://doi.org/10.3390/textiles6020053 - 30 Apr 2026
Viewed by 502
Abstract
This review examines how plant polyphenols enable multifunctional textiles, offering a sustainable alternative to synthetic dyes and nanomaterial-based treatments. A literature search (2001–2025) identified 105 peer-reviewed studies across eight functional areas. Abundant in agricultural and industrial byproducts, plant polyphenols act as natural colorants, [...] Read more.
This review examines how plant polyphenols enable multifunctional textiles, offering a sustainable alternative to synthetic dyes and nanomaterial-based treatments. A literature search (2001–2025) identified 105 peer-reviewed studies across eight functional areas. Abundant in agricultural and industrial byproducts, plant polyphenols act as natural colorants, bio-adhesives, and performance enhancers—providing coloration, antibacterial activity, UV protection, flame retardancy, deodorization, antioxidant capacity, superhydrophobicity, and more. Their catechol and pyrogallol groups bind strongly to natural and synthetic fibers via hydrogen bonding, π–π stacking, and metal chelation, ensuring durable, nontoxic functionality. We analyze structure–function links and scalable methods, including pad-dry-cure and metal–phenolic network (MPN) assembly, which were validated against ISO, ASTM, and AATCC standards. Polyphenol-based textiles match or exceed conventional ones in key metrics, with added benefits: full biodegradability, low ecotoxicity, and skin compatibility. Key advances include enzymatic polymerization for wash-stable color, MPN tuning for customizable functions, and using waste-derived polyphenols. However, major challenges remain: narrow color range (mostly yellow, brown, black) and poor wash/UV resistance, leading to rapid fading and loss of antibacterial/UV protection after laundering. Solving these is a top priority for future work. Overall, this review delivers a practical, science-based roadmap for high-performance, sustainable textiles that align with the Sustainable Development Goals and meet real-world needs in healthcare, sportswear, and smart wearables. Full article
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31 pages, 1178 KB  
Review
Low-Cost, Sustainable Materials and 3D-Printed Systems for Wastewater Treatment and Reuse in Rural Communities: A Critical Review
by Wathsala Benthota Pathiranage, Hunain Alkhateb and Matteo D’Alessio
Water 2026, 18(9), 1002; https://doi.org/10.3390/w18091002 - 23 Apr 2026
Viewed by 511
Abstract
Global water scarcity has intensified over recent decades, with projections suggesting that nearly six billion people may face limited access to clean water by 2050. Water reuse has emerged as a viable strategy to alleviate pressure on freshwater resources, particularly for non-potable applications. [...] Read more.
Global water scarcity has intensified over recent decades, with projections suggesting that nearly six billion people may face limited access to clean water by 2050. Water reuse has emerged as a viable strategy to alleviate pressure on freshwater resources, particularly for non-potable applications. However, safe implementation requires wastewater to be treated to meet fit-for-purpose quality standards established through regional and national regulatory frameworks. Despite high levels of basic sanitation coverage in high-income countries such as the United States, persistent gaps remain in affordable and equitable wastewater management, particularly in small and underserved communities. This review focused on current knowledge of sustainable low-cost materials, including plant-based, clay, and clay-based ceramics; animal-derived products; and industrial by-products, used to remove a broad range of contaminants, including heavy metals, dyes, nutrients, emerging contaminants, and pathogens, from wastewater. The mechanisms governing their performance, such as adsorption, coagulation–flocculation, and filtration, were examined alongside contaminant-specific performance. The review further highlights the emerging role of 3D printing in developing customizable, efficient, and scalable treatment units using low-cost or waste-derived materials. Life cycle assessment (LCA) studies were evaluated to highlight their role as a flexible framework for assessing environmental impacts across life-cycle stages and for guiding the selection of sustainable materials and treatment systems. Together, these perspectives provide a comprehensive foundation for developing decentralized, community-oriented wastewater treatment solutions that support safe and effective water reuse, especially in rural and small communities. Full article
(This article belongs to the Section Wastewater Treatment and Reuse)
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24 pages, 7630 KB  
Article
Bio-Based Pigment Water-Based Inks for Piezoelectric Inkjet Printing
by Jéssica Antunes, Agata Nolasco, Beatriz Marques, Marisa Lopes, Philippe Sarra-Bournet, Augusta Silva, Helena Vilaça and Carla J. Silva
Colorants 2026, 5(2), 13; https://doi.org/10.3390/colorants5020013 - 13 Apr 2026
Cited by 1 | Viewed by 346
Abstract
The development of bio-based inks represents a promising strategy to reduce the environmental impact of digital printing technologies. This study investigates the formulation and performance of water-based inks incorporating two renewable pigments: a fermentation-derived indigo pigment and a plant-extracted yellow pigment. Special attention [...] Read more.
The development of bio-based inks represents a promising strategy to reduce the environmental impact of digital printing technologies. This study investigates the formulation and performance of water-based inks incorporating two renewable pigments: a fermentation-derived indigo pigment and a plant-extracted yellow pigment. Special attention was given to dispersion optimization of the poorly water-soluble indigo pigment. Extended mechanical dispersion (115 h in a ball mill) proved critical to achieve colloidal stability, enabling the preparation of inks that met standard rheological and physicochemical criteria for inkjet printing with piezoelectric printheads. Both inks were applied on a variety of substrates, including cotton, polyester, leather, and kraft paper, pre-treated, in the case of the textiles, with either a cationic biopolymer or a synthetic polyurethane-based binder. Colorimetric evaluation confirmed effective deposition and uniformity, with the indigo ink producing deep blue hues and superior overall fastness than the yellow ink, particularly in washing and rubbing tests. The yellow pigment ink showed good stability but once applied to the fabric, the resulting print exhibited poor fastness, particularly against light exposure, indicating limited durability of the coloration on the textile. Shelf-life analysis of the indigo ink revealed a decline in viscosity and surface tension over time, though the colour and particle size remained stable, particularly under room temperature conditions. These findings confirm the potential of fermentation-derived indigo as a robust bio-based alternative to synthetic dyes and its superior performance in relation to other nature extracted pigments, which, although facilitating ink preparation due to their higher water solubility, result in lower-fastness prints. Full article
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40 pages, 2163 KB  
Systematic Review
Plant Extracts as Antibacterial and Antifungal Agents in Medical Textiles: A Systematic Review of Key Components, Efficacy, and Application Techniques
by Praxedes Jeanpierre Merino-Ramirez and Rebeca Salvador-Reyes
Resources 2026, 15(4), 52; https://doi.org/10.3390/resources15040052 - 30 Mar 2026
Viewed by 1443
Abstract
This systematic review examines the use of plant-derived extracts as antibacterial and antifungal agents in medical textiles, with an emphasis on active components, extraction techniques, biological efficacy, target microorganisms, and fabric application methods. This study is framed within the context of natural resource-based [...] Read more.
This systematic review examines the use of plant-derived extracts as antibacterial and antifungal agents in medical textiles, with an emphasis on active components, extraction techniques, biological efficacy, target microorganisms, and fabric application methods. This study is framed within the context of natural resource-based plant biomass and agro-industrial residues as a sustainable source of high-value functional compounds for resource valorization. Searches in Scopus and Web of Science followed the PIOC framework and PRISMA protocol. From an initial 389 records, 38 studies met the eligibility criteria. We identified a sustained growth in publications between 2020 and 2025, and six predominant thematic lines related to medical textiles, sustainability, antimicrobial assessment, structural characterization, natural dyeing optimization, and antioxidant functionalization. Among the most studied species, Aloe barbadensis and Salvia officinalis were prominent. Leaves were the most frequently used plant organ, highlighting their relevance as readily available renewable biomass resources. Maceration was the most common extraction method, although ultrasound-assisted extraction yielded a broader metabolite profile and better preserved thermolabile compounds, demonstrating the impact of biomass conversion techniques on resource efficiency and extract quality. Cotton 100% (plain weave) was the most widely used substrate, and the exhaustion method (immersion/exhaust dyeing) was the preferred application technique. Overall, plant extracts obtained through the sustainable management and valorization of plant resources achieved high inhibition against pathogenic bacteria, including resistant strains, and consistent antifungal activity, supporting their potential for developing functional and sustainable medical textiles. These findings align with the goals for responsible production and good health and well-being and reinforce the role of biomass-based resource systems within a circular bioeconomy, opening avenues to optimize formulations, standardize methodologies, and evaluate post-laundering performance and in vivo biocompatibility. Full article
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12 pages, 581 KB  
Article
Paediatric Dermatology Insights for Functional Fashion Textile Design
by Diana Santiago, Sofia Moreira, Isabel Cabral, Paulo Mendes and Joana Cunha
Textiles 2026, 6(2), 38; https://doi.org/10.3390/textiles6020038 - 24 Mar 2026
Viewed by 481
Abstract
Children’s skin is uniquely vulnerable, requiring specialised design solutions that transcend traditional aesthetics. This exploratory study investigates the importance of paediatric dermatology in informing functional fashion design through expert medical perspectives. Using a qualitative approach, data were gathered from a purposive cohort of [...] Read more.
Children’s skin is uniquely vulnerable, requiring specialised design solutions that transcend traditional aesthetics. This exploratory study investigates the importance of paediatric dermatology in informing functional fashion design through expert medical perspectives. Using a qualitative approach, data were gathered from a purposive cohort of paediatric dermatologists and immunoallergologists and analysed through inductive thematic analysis. Findings identify four core themes: the physiological immaturity of children’s skin (notably the prevalence of atopic dermatitis), clothing’s role as a symptomatic aggravator rather than a primary aetiology, the clinical risks posed by chemical additives in synthetic textile processes, and the therapeutic potential of natural fibres and biofunctional agents. The data also highlights significant diagnostic constraints in paediatric patch testing, emphasising the necessity of proactive material safety. The findings suggest that integrating healthcare expertise into human-centred design may support the development of safer paediatric clothing solutions, ensuring that fashion industry innovation meets the physiological requirements of children. By transitioning from hazardous synthetic processes to biocompatible textiles, such as undyed natural fibres and medicinal plant-derived dyes, the industry can transform apparel from a potential irritant into a secondary protective barrier. This provides initial insights for developing clothing that safeguards the skin barrier and improves the overall wellbeing of vulnerable populations. Full article
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63 pages, 12604 KB  
Review
A Comprehensive Review on Green Synthesis and Characterization of Plant-Based Nanoparticles for Water Treatment Applications: Adsorption and Photodegradation of Organic and Inorganic Pollutants
by Marouane El Alouani, Hamid Saufi, Badr Aouan, Rajaa Bassam, Mariem Ben Tourtit, Amal Bassam, Wafaa Ahmina, Younes Rachdi, Said Belaaouad and Saliha Alehyen
Sustainability 2026, 18(6), 2721; https://doi.org/10.3390/su18062721 - 11 Mar 2026
Cited by 1 | Viewed by 1088
Abstract
Growing concerns about environmental pollution and the sustainability of conventional nanomaterial synthesis have accelerated interest in plant-based routes for nanoparticle production. This review provides an in-depth analysis of more than 290 peer-reviewed research and review articles published between 2010 and 2025, extracted from [...] Read more.
Growing concerns about environmental pollution and the sustainability of conventional nanomaterial synthesis have accelerated interest in plant-based routes for nanoparticle production. This review provides an in-depth analysis of more than 290 peer-reviewed research and review articles published between 2010 and 2025, extracted from the Web of Science and Scopus databases, on the green synthesis of metallic and metal oxide nanoparticles using plant extracts, with particular emphasis on their characterization and application in water treatment. Plant-derived phytochemicals serve as natural reducing and stabilizing agents, enabling nanoparticle formation without hazardous reagents. Key physicochemical characterization techniques, including UV–Visible spectroscopy, X-ray diffraction, Fourier Transform Infrared spectroscopy, scanning and transmission electron microscopy, and energy-dispersive X-ray analysis, are evaluated for their roles in confirming nanoparticle structure, morphology, surface chemistry, and optical behavior. The review focuses on water purification applications, highlighting adsorption and photocatalytic degradation as the most extensively investigated removal pathways. Particular attention is given to widely studied material classes such as silver, zinc oxide, titanium dioxide, and iron-based nanoparticles, which demonstrate effective removal of heavy metals, synthetic dyes, pesticides, and pharmaceutical residues. Current limitations related to synthesis reproducibility, mechanistic understanding, stability, and scalability are critically discussed. The review concludes by identifying priority research directions, including standardized synthesis protocols, deeper chemical analysis of plant extracts, and the integration of green nanoparticles into immobilized and membrane-based systems to advance their practical implementation in sustainable water treatment technologies. Full article
(This article belongs to the Section Sustainable Water Management)
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23 pages, 4004 KB  
Article
Biocompound and Lake Pigment Extraction from Invasive Alien Plant Biomass for Sustainable Ink Applications
by Antía Iglesias, Ángeles Cancela, Anne Heyvaert and Ángel Sánchez
Appl. Sci. 2026, 16(6), 2635; https://doi.org/10.3390/app16062635 - 10 Mar 2026
Viewed by 437
Abstract
The management of invasive alien species (IAS) generates large amounts of plant waste biomass that is commonly disposed of by burning or destruction, leading to environmental and economic drawbacks. At the same time, the production of synthetic dyes and pigments used in printing [...] Read more.
The management of invasive alien species (IAS) generates large amounts of plant waste biomass that is commonly disposed of by burning or destruction, leading to environmental and economic drawbacks. At the same time, the production of synthetic dyes and pigments used in printing and graphic applications remains a significant source of pollution. In this context, the valorization of IAS biomass as a source of natural colorants represents a sustainable alternative aligned with circular economy principles. Here, biocompounds and natural dyes were extracted from four invasive or non-native plant species—Arundo donax, Phytolacca americana, Tradescantia fluminensis, and Eucalyptus globulus—using five solid–liquid extraction methods: infusion, infusion with heat, thermal agitation, Soxhlet extraction, and ultrasonic-assisted extraction. Extraction efficiency and color preservation were comparatively evaluated. Although Soxhlet extraction provided the highest extraction yield (up to 30.5%), infusion with heat proved to be the most suitable method for preserving color integrity and minimizing oxidation. Liquid dyes obtained by the selected extraction method were converted into solid pigments through a lake pigment precipitation process using aluminum potassium sulfate and sodium bicarbonate. The resulting pigments were characterized in terms of chemical composition, particle size, and chromatic properties, and subsequently formulated into oil-based inks using linseed oil as binder. Scanning electron microscopy revealed pigment particle sizes ranging from approximately 2.1 to 8.3 µm, depending on the plant source, and confirmed adequate ink penetration and distribution on commercial printmaking paper. The obtained pigments exhibited color tones ranging from yellow to brown and grey, mainly associated with the phenolic and tannin content of the original biomass. Printing tests demonstrated the suitability of the developed inks for manual printmaking techniques, highlighting the potential of IAS-derived pigments as sustainable alternatives for artistic and printing applications. Full article
(This article belongs to the Section Chemical and Molecular Sciences)
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48 pages, 7674 KB  
Review
Textile Microplastics in Wastewater: A Critical Review of Removal and Carbonization Technologies
by Azam Ali and Muhammad Zaman Khan
C 2026, 12(1), 24; https://doi.org/10.3390/c12010024 - 9 Mar 2026
Viewed by 2379
Abstract
The rapid growth of synthetic textile production has intensified the release of micro- and nanoplastics (MPs/NPs) into aquatic environments, primarily through industrial effluents and domestic laundering. Textile-derived microplastics, especially polyester fibers and polymeric coating fragments, constitute a significant fraction of plastic contamination in [...] Read more.
The rapid growth of synthetic textile production has intensified the release of micro- and nanoplastics (MPs/NPs) into aquatic environments, primarily through industrial effluents and domestic laundering. Textile-derived microplastics, especially polyester fibers and polymeric coating fragments, constitute a significant fraction of plastic contamination in wastewater systems. Although wastewater treatment plants (WWTPs) can remove a large proportion of MPs, substantial quantities accumulate in sewage sludge, raising concerns about long-term environmental persistence and secondary release pathways. This review critically examines the sources, classification, and release mechanisms of textile-based micro- and nanoplastics, including fibrous debris and coating-derived fragments. Then it focuses on current identification and removal technologies, such as sedimentation, coagulation/flocculation, electrocoagulation, flotation, membrane filtration, adsorption, and biodegradation, and on the emerging strategy of converting recovered microplastics into value-added porous carbon materials via hydrothermal treatment and pyrolysis. Carbonized microplastics exhibit high surface area and adsorption capacity for dyes, heavy metals, and organic pollutants, offering a circular approach that simultaneously mitigates plastic pollution and enhances wastewater treatment efficiency. By integrating source control, optimized removal technologies, and carbonization-based valorization, this review proposes a dual-benefit framework that transforms textile-derived microplastic waste from an environmental liability into a functional resource for sustainable water purification. Full article
(This article belongs to the Section Carbon Materials and Carbon Allotropes)
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24 pages, 4365 KB  
Article
Traditional Medicine Extracts of Gnidia sericocephala and Product Nkabinde in HIV-1 Latency Reversal: Insights from J-Lat Subtype B and J-Lat Subtype C Models
by Khanyisile Mngomezulu, Samukelisiwe Pretty Khathi, Siphathimandla Authority Nkabinde, Magugu Nkabinde, Mlungisi Ngcobo and Nceba Gqaleni
Int. J. Mol. Sci. 2026, 27(3), 1581; https://doi.org/10.3390/ijms27031581 - 5 Feb 2026
Viewed by 838
Abstract
The persistence of latent HIV-1 reservoirs in individuals on antiretroviral therapy (ART) remains a major barrier to cure, necessitating strategies such as “shock and kill” using latency-reversing agents (LRAs). However, current LRAs show limited clinical efficacy, highlighting the need for novel interventions. This [...] Read more.
The persistence of latent HIV-1 reservoirs in individuals on antiretroviral therapy (ART) remains a major barrier to cure, necessitating strategies such as “shock and kill” using latency-reversing agents (LRAs). However, current LRAs show limited clinical efficacy, highlighting the need for novel interventions. This study evaluated the in vitro latency-reversing potential of Product Nkabinde (PN) and Gnidia sericocephala using J-Lat A2 (subtype B) and J-Lat C clones T66 and T17 (subtype C) cells. Cell viability was assessed using flow cytometry with Live/Dead dye. Reactivation potential was further tested in combination with established LRAs: panobinostat, SAHA, and TNF-α. G. sericocephala induced dose-dependent latency reversal, with 26.1% of J-Lat A2 and 15.8% of J-Lat T66 cells GFP-positive at 106 µg/mL (p = 0.0001). Co-treatment with LRAs enhanced reactivation—34.6% with SAHA and 87.2% with TNF-α in J-Lat A2 cells, and 56.9% with SAHA and 65.4% with TNF-α in J-Lat T66 cells (p = 0.0001)—while maintaining cell viability above 90%. PN showed minimal activity (≤1.3% GFP-positive) and no effect in combination assays. Fractional inhibitory concentration index analysis revealed no synergistic interactions. Ex vivo, PN and G. sericocephala induced limited increases in HIV-1 gag RNA without substantial cytotoxicity. These findings demonstrate that G. sericocephala effectively reverses HIV-1 latency and potentiates TNF-α-induced reactivation, supporting its potential as a plant-derived LRA for future “shock and kill” HIV-1 cure strategies. Full article
(This article belongs to the Section Molecular Immunology)
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28 pages, 4312 KB  
Review
From Biomass to Adsorbent: A Comprehensive Review on Bio-Derived Carbons for Dye Removal
by Buvaneswari Kuppusamy, Fathima Rigana Mohamed Ismail, Preethi Balakrishnan, Seong-Cheol Kim, Shakila Parveen Asrafali and Thirukumaran Periyasamy
Polymers 2026, 18(2), 180; https://doi.org/10.3390/polym18020180 - 9 Jan 2026
Cited by 9 | Viewed by 1731
Abstract
The escalating release of synthetic dyes from textile and allied industries has become a pressing global environmental issue due to their toxicity, persistence, and resistance to biodegradation. Among the various treatment strategies, adsorption has emerged as one of the most efficient, economical, and [...] Read more.
The escalating release of synthetic dyes from textile and allied industries has become a pressing global environmental issue due to their toxicity, persistence, and resistance to biodegradation. Among the various treatment strategies, adsorption has emerged as one of the most efficient, economical, and sustainable techniques for dye removal from aqueous environments. This review highlights recent advances in bio-derived adsorbents—particularly raw biomass powders, biochars, and activated carbons—developed from renewable waste sources such as agricultural residues, fruit peels, shells, and plant fibers. It systematically discusses adsorption mechanisms, the influence of process parameters, kinetic and thermodynamic models, and regeneration performance. Furthermore, the review emphasizes the superior adsorption efficiency and cost-effectiveness of biomass-derived carbons compared to conventional adsorbents. The integration of surface modification, magnetization, and nanocomposite formation has further enhanced dye uptake and reusability. Overall, this study underscores the potential of biomass-derived materials as sustainable alternatives for wastewater treatment and environmental remediation. Full article
(This article belongs to the Topic Advances in Carbon-Based Materials)
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21 pages, 2750 KB  
Article
Eco-Friendly Synthesis of ZnO-Based Nanocomposites Using Haloxylon and Calligonum Extracts for Enhanced Photocatalytic Degradation of Methylene Blue
by Elham A. Alzahrani, Sabri Ouni, Mohamed Bouzidi, Abdullah S. Alshammari, Ahlam F. Alshammari, Rizwan Ali, Odeh A. O. Alshammari, Naim Belhaj Mohamed and Noureddine Chaaben
J. Compos. Sci. 2026, 10(1), 18; https://doi.org/10.3390/jcs10010018 - 4 Jan 2026
Cited by 1 | Viewed by 1016
Abstract
This study presents a green synthesis of zinc oxide (ZnO) nanoparticles (NPs) capped with Haloxylon (P1) and Calligonum (P2) extracts. The use of plant-derived biomolecules as natural capping agents offers an environmentally friendly strategy to tune surface chemistry and to enhance the photocatalytic [...] Read more.
This study presents a green synthesis of zinc oxide (ZnO) nanoparticles (NPs) capped with Haloxylon (P1) and Calligonum (P2) extracts. The use of plant-derived biomolecules as natural capping agents offers an environmentally friendly strategy to tune surface chemistry and to enhance the photocatalytic behavior of ZnO NPs. ZnO/plant extracts nanocomposites were prepared via a hydrothermal route and systematically characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), UV–Vis spectroscopy, and photoluminescence (PL), followed by evaluation of their photocatalytic performance against methylene blue (MB) under UV irradiation. XRD confirmed a wurtzite structure with crystallite sizes ranging from 8.95 to 10.93 nm, while PL spectra indicated an improved charge carrier separation in extract-capped ZnO. The characteristics and pollutant removal performance of the greenly synthesized ZnO composites were compared with those of a chemically synthesized ZnO nanoparticles reference sample. Adsorption tests under dark conditions revealed a strong difference between the materials: ZnO-P1 removed 48% of MB, whereas ZnO-P2 adsorbed only 7%, demonstrating a much higher affinity of the Haloxylon-derived surface groups toward MB. In comparison, the chemically synthesized ZnO exhibited an adsorption capacity of 54%, confirming that the Haloxylon-mediated surface provides a comparable efficient dye uptake prior to irradiation. After UV irradiation, all samples exhibited a photocatalytic activity with a total MB removal reached ~59% for the reference ZnO sample and ~53% for ZnO-P1 compared to about 13% for the ZnO-P2. Kinetic analysis also confirmed that ZnO-P1 possessed a high degradation rate constant, indicating a better intrinsic photocatalytic efficiency in addition to the strong adsorption contribution. The enhanced performance of plant-capped ZnO is attributed to phytochemical-induced surface defects, which facilitated charge separation and boosted the generation of reactive oxygen species (ROS). Overall, these results demonstrate that Haloxylon and Calligonum extracts are effective and sustainable capping agents, providing a low-cost, eco-friendly approach for designing ZnO nanocatalysts composites with promising applications in wastewater treatment and environmental remediation. Full article
(This article belongs to the Special Issue Composites: A Sustainable Material Solution, 2nd Edition)
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14 pages, 2120 KB  
Article
Novel Method for Characterizing Humic Substances Using Fluorescent Solvatochromism
by Kazuto Sazawa, Hanae Koyama, Yusuke Yamazaki, Yoshiki Hara, Nozomi Kohama, Yustiawati Yustiawati and Hideki Kuramitz
Sensors 2026, 26(1), 107; https://doi.org/10.3390/s26010107 - 23 Dec 2025
Viewed by 881
Abstract
Charge-transfer-type fluorochromes, which exhibit shifts in fluorescence intensity and emission wavelength in response to solvent polarity changes, have been widely employed to investigate solute–solvent interactions. Humic substances (HSs) are naturally occurring macromolecular organic acids derived from plant residues, with structural properties that vary [...] Read more.
Charge-transfer-type fluorochromes, which exhibit shifts in fluorescence intensity and emission wavelength in response to solvent polarity changes, have been widely employed to investigate solute–solvent interactions. Humic substances (HSs) are naturally occurring macromolecular organic acids derived from plant residues, with structural properties that vary depending on their origin and environmental conditions. The polarity of HSs is closely associated with the mobility and toxicity of environmental pollutants, making their chemical characterization essential. In this study, we developed a rapid and straightforward method to characterize HS polarity using fluorescent solvatochromism. The fluorescence peak shifts of four dyes—8-anilino-1-naphthalenesulfonic acid (ANS), acridine orange (AO), methylene blue (MB), and Rhodamine B (RhB)—were evaluated in the presence of humic acids (HAs), a major component of HSs. To assess environmental variability, a total of twelve HS samples were tested, including HSs derived from soils of different origins, compost, commercial reagents, and standard reference materials. Among these, AO and MB exhibited distinct spectral shifts without overlapping with the intrinsic fluorescence of HAs. Notably, MB displayed a consistent blue shift dependent on HA concentration, with the most stable response observed at 5 mg/L. The magnitude of this shift was significantly correlated with UV–Vis parameters associated with the aromaticity, humification degree, and polarity of HSs. Overall, this study demonstrates that MB-based fluorescent solvatochromism can function as an empirical and facile indicator for assessing the structural and microenvironmental characteristics of HSs, providing a rapid and complementary screening approach for HSs extracted and purified from environmental samples. Full article
(This article belongs to the Special Issue Colorimetric and Fluorescent Sensors and Their Application)
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17 pages, 3986 KB  
Article
Polyphenols from Inula oculus-christi L. Induced Cell-Specific Membrane and Cytoskeleton Reorganization
by Ralitsa Veleva, Aneliya Kostadinova, Antoaneta Trendafilova, Viktoria Ivanova, Veselina Moskova-Doumanova, Kirilka Mladenova, Jordan Doumanov, Dayana Benkova, Galya Staneva and Tanya Topouzova-Hristova
Membranes 2025, 15(12), 357; https://doi.org/10.3390/membranes15120357 - 26 Nov 2025
Viewed by 852
Abstract
Interrelations between the plasma membrane and cytoskeleton are of crucial importance for essential cellular processes such as endocytosis, formation of intercellular junctions, cell morphology, etc. Many studies validate the beneficial effects of polyphenols as antioxidant and protective agents, but a molecular mechanism of [...] Read more.
Interrelations between the plasma membrane and cytoskeleton are of crucial importance for essential cellular processes such as endocytosis, formation of intercellular junctions, cell morphology, etc. Many studies validate the beneficial effects of polyphenols as antioxidant and protective agents, but a molecular mechanism of their interaction and transition through the plasma membranes of different cell lines is still missing. In this study, we examined the affinity of fractions enriched in flavonoid glycosides (FGs) and caffeoylquinic acids (CQAs), obtained from the methanol extract of the medicinal plant Inula oculus-christi L., to reorganize the plasma membrane structure and actin cytoskeleton by using confocal microscopy. Assessment of the degree of membrane ordering aiming to distinguish the ordered from disordered regions of the cellular membranes was performed using the fluorescent dye Di-4-ANEPPDHQ, and visualization of F-actin was by TRITC-phalloidin. Two epithelial cell lines with clear differences in their origin and plasma membrane organization were chosen: the non-malignant MDCK II and the cancerous A549. Our results showed that flavonoid glycosides exhibited an ordering effect on plasma membranes of cancerous cells and fluidized one on non-malignant cells. Different patterns of actin reorganization were observed for both cell lines after treatment. Our results indicate the potential of plant-derived polyphenols as modulators of the membrane’s structural organization, offering valuable insights for the development of membrane-targeted therapeutic strategies. Full article
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22 pages, 3940 KB  
Article
Rapid Detection of Animal-Derived Components in Plant-Based Meat Alternatives Using Recombinase Polymerase Amplification
by Yifei Sun, Han Li, Tianqi Ma, Tingting Xie, Yuqin Ni, Yu Chen, Xinya Chen, Wenke Ding and Zhuqing Xing
Foods 2025, 14(23), 3992; https://doi.org/10.3390/foods14233992 - 21 Nov 2025
Viewed by 904
Abstract
Plant-based meat alternatives (PMAs), as an emerging food category gaining increasing popularity, face potential food safety risks and ethical concerns for vegetarians due to the illegal adulteration of animal-derived components. To address these challenges and enhance regulatory oversight, the development of a rapid, [...] Read more.
Plant-based meat alternatives (PMAs), as an emerging food category gaining increasing popularity, face potential food safety risks and ethical concerns for vegetarians due to the illegal adulteration of animal-derived components. To address these challenges and enhance regulatory oversight, the development of a rapid, sensitive, and highly specific detection method is essential. In this study, five DNA extraction methods were evaluated and optimized to identify the most effective approach for PMA products. The optimal conditions were determined to be 60 mmol/L NaCl, 10 mmol/L Tris HCl, and a centrifugation speed of 12,000× g. Additionally, specific primers targeting four common animal-derived adulterants, namely pork, chicken, duck, and beef, were designed and screened for targeted amplification. To establish a rapid and visually interpretable detection system, the recombinant polymerase amplification conditions were optimized. The final protocol used 0.4 µmol/L primer and isothermal amplification at 39 °C for 25 min, with the incorporation of SYBR Green I dye enabling the rapid and specific visualization of animal-derived DNA. This optimized method is characterized by its simplicity, sensitivity (capable of detecting beef-derived components as low as 0.0514% w/w), and rapidity, significantly reducing detection time and providing a reliable tool for the identification of animal-derived adulteration in PMA products. Full article
(This article belongs to the Section Food Analytical Methods)
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