Search Results (109)

Background/Objectives: A push–pull osmotic pump (PPOP) tablet is a controlled-release delivery system consisting of a bilayer tablet (push layer/pull (drug) layer), coated within a selectively permeable barrier membrane, and containing a laser-drilled orifice in the pull layer side of the barrier. Aqueous media permeates across the barrier membrane and into the bilayer tablet core. The push layer swells axially, creating force and acting like a piston to drive the active pharmaceutical ingredient (API) dosage through the laser-drilled orifice. The primary purpose of this study was to develop a method to quantitate swelling force generated by the PPOP push layer. Methods: A key challenge was devising measurement methodology that most closely represented continuous PPOP push-layer swelling force exerted in the axial dimension over time, so the method setup was adjusted until attaining what closely approximated PPOP push-layer swelling-force dynamics within the delivery system. After some adjustments, swelling forces were measured from PPOP push layers containing various polyethylene oxide (PEO) molecular weight (MW) grades. Results: The most representative configuration was a cylindrical holder fully submerged in water with the push-layer compact situated at the bottom of the holder. This setup constrained the push layer to swell in the axial dimension. The optimal setup also allowed the texture analyzer to continuously measure push-layer swelling force over 24 h, representing typical duration of functionality. Quantitative analysis of the swelling force vs. time profiles demonstrated comparable force generation over 24 h from the 4, 5, and 7,000,000 MW grades of PEO. Swelling force decreased in near-linear fashion, from push layers formulated with PEO 4,000,000 down to PEO 100,000. Conclusions: The optimal setup enabled discriminatory quantification of push-layer swelling force for PEO MW grades spanning from 100,000 up to 4,000,000 Daltons. Furthermore, the method demonstrated why the 4, 5, and 7,000,000 MW grades of PEO are recommended for use in the PPOP push layer, given that these three highest MW grades delivered comparable swelling force over 24 h. Finally, the method provides new quantitative insight into the push-layer swelling force that is necessary, over the duration of the dosing interval, to deliver the active dosage from the PPOP tablet. Full article

Freshwater scarcity and rising wastewater generation have intensified global reliance on desalination and reuse, with reverse osmosis (RO) providing 65–70% of installed desalination capacity and achieving energy reductions from 15 kWhm⁻³ in the 1970s to 1.8–2.5 kWhm⁻³ today. However, fouling caused by organics, inorganics, microorganisms, and colloids remains the major operational challenge, accounting for ≈25% of RO costs and over USD 15 billion annually. This review synthesises fouling mechanisms and mitigation strategies in renewable energy (RE)-driven RO wastewater-treatment systems, where intermittency exacerbates fouling through start–stop cycles and low-shear conditions. Analysis of recent literature highlights that mixed fouling reduces flux by 10–30%, increases transmembrane pressure, and deteriorates permeate quality. Advances in pretreatment (coagulation, MF/UF), antifouling membranes (hydrophilic coatings, zwitterionic surfaces), and cleaning protocols (osmotic backwashing, nanobubbles) have improved performance, yet complete prevention remains elusive. Persistent gaps include predictive fouling models, standardised performance metrics, and scalable green chemistries for silica and combined fouling control. Future directions emphasise integrated solutions combining advanced materials, AI-driven monitoring, and renewable-aware operational strategies, alongside circular economy approaches for brine valorisation. These innovations are critical for achieving sustainable, low-carbon RO systems for global water security. Full article

Background and Objectives: Acute rhinosinusitis is associated with a substantial deterioration in quality of life, including symptoms such as nasal obstruction, facial pressure, cough, and sleep disruption. Growing interest in non-pharmacological management strategies has been observed, particularly among populations in whom systemic treatments may be limited, such as pediatric patients and pregnant individuals. NESOSPRAY HE-G is a nasal device containing a filmogenic glycerol formulation designed to create a protective barrier and induce an osmotic effect, thereby promoting nasal decongestion and mucosal protection. Materials and Methods: A randomized, double-blind, placebo-controlled study was conducted to assess the performance and safety of NESOSPRAY HE-G (n = 20) compared with a saline comparator (n = 20) in patients aged 3 years and older presenting with acute rhinosinusitis and a baseline Rhino-Sinusitis Severity Score (RSSS) of at least 25/50. The investigational product was administered as three sprays per nostril, four times daily, over a maximum period of 15 days or until symptom resolution. Efficacy was primarily evaluated through changes in the total RSSS and in key symptoms, including nasal congestion, facial pain, cough, sleep disturbance, and fever. Safety assessments included monitoring adverse events (AE) and evaluating local tolerability. Results: Baseline characteristics were comparable between groups. Treatment with NESOSPRAY HE-G resulted in a statistically significant reduction in total RSSS from Day 2 onwards, with sustained effects throughout the study period. Improvements were also observed across individual symptoms, including early relief of nasal congestion and reductions in cough and facial pain, with faster onset compared to the comparator. Sleep quality showed progressive improvement. Resolution of fever occurred in both groups, with an earlier effect observed in the NESOSPRAY HE-G arm. No serious adverse events (SAE) or hypersensitivity reactions were reported. The product demonstrated good tolerability. Conclusions: NESOSPRAY HE-G provides rapid and sustained alleviation of symptoms associated with acute rhinosinusitis. Its topical mode of action, based on osmotic activity and barrier formation, represents a suitable non-pharmacological option, particularly for patients seeking alternatives to systemic therapies or presenting contraindications to such treatments. Full article

In the development of carbonate reservoirs via water flooding huff-n-puff, the osmotic pressure effect is frequently overlooked, and existing models inadequately quantify the matrix imbibition and oil expulsion driven by salinity gradients. To address this issue, this study establishes a coupled oil–water two-phase huff-n-puff flow model for carbonate reservoirs that incorporates the interplay between salt concentration and osmotic pressure, which, for the first time, fully couples the van ’t Hoff osmotic pressure equation with solute transport equations for fractured-vuggy carbonate huff-n-puff, filling the gap that prior tight/shale reservoir low-salinity flow models fail to adapt to cyclic injection-soaking production regimes of carbonates. Based on the IMPES (implicit pressure–explicit saturation) numerical simulation method, an equivalent single-nucleus model is adopted to characterize the fractured-vuggy reservoir architecture. The model integrates the osmotic pressure formula, solute transport equation, and two-phase seepage governing equations, enabling a systematic analysis of the mechanisms by which osmotic pressure affects the multi-stage seepage process and the influence of key parameters on development performance. Quantitative simulation reveals three core laws controlled by salinity-induced osmosis: first, osmotic pressure drives water molecules to spontaneously migrate from the high-permeability fracture inner core toward the tight matrix pores, thereby modifying the water saturation distribution, expanding the water sweep region, and smoothing the saturation gradient between the inner and outer cores, which effectively mitigates water channeling in fractured reservoirs. Under the base case (injected water salinity = 1000 mg/L, inner-core permeability = 1000 mD, shut-in time = 80 d), the oil recovery factor with osmotic pressure considered reaches 13.46%, representing a 3.50% increment over the case without osmotic pressure. The recovery factor decreases monotonically with increasing injected water salinity, while it increases with longer shut-in time and higher inner-core permeability, both exhibiting pronounced diminishing marginal returns; the optimal shut-in time is approximately 80 d under the simulated conditions. This work delivers a fully coupled numerical tool and quantitative evaluation standard for osmotic imbibition mechanisms in fractured-vuggy carbonates. The quantified recovery increment and optimal soaking window established herein can directly guide field parameter optimization of injection water salinity, shut-in cycle and fracture reconstruction scale, balancing oil increment revenue and water treatment/well shutdown operation costs for on-site low-salinity huff-n-puff design. Full article

Transforming traditional membrane bioreactors (MBRs) into forward osmosis membrane bioreactors (OMBRs) is a highly challenging yet promising technological upgrade. Although both combine biological treatment and membrane separation, their core driving forces and operating mechanisms are completely different (an MBR is pressure-driven, while an OMBR is osmosis-driven). In this paper, a novel OMBR with an integrated fixed biofilm (BF-OMBR) was tested for the treatment of synthetic rural wastewater using fertilizer potassium chloride (KCl) as the draw solution (DS) and a commercial aquaporin Inside^TM forward osmosis (FO) membrane. A bench-scale investigation was conducted to compare the BF-OMBR with traditional OMBRs and MBRs. The experimental data suggested that the reactor with aquaporin membranes contributed to a higher water flux than traditional TFC membranes, while immobilized biofilms improved the total nitrogen removal rate compared to normal OMBRs. The integration of these two technologies in the BF-OMBR system appears to leverage these individual benefits. Its TOC and ammonia nitrogen removal efficiencies were also better than those of the other two bioreactors. Meanwhile, the BF-OMBR successfully controlled the salinity build-up to a level not exceeding 2.5 mS/cm over 90 days of operation. This novel osmotic bioreactor may represent a possible alternative approach to overcoming the challenges of low-temperature and low-C/N-ratio rural sewage treatment. Full article

Mediterranean coastal groundwater-dependent ecosystems are among the most vulnerable environments to the combined effects of climate change and local anthropogenic pressures, yet long-term quantitative assessments disentangling these drivers remain limited. The 41-year hydro-ecological dynamics (1984–2025) of “Le Soglitelle”, a transitional man-made coastal GDE located in the Campania Plain (southern Italy), were reconstructed across three management regimes: illegal hunting via electric pumps augmentation of flooded areas (1984–2004), post-seizure transition (2005–2015), and fenced natural reserve sustained by artesian wells flow (2016–2025). A monthly multi-sensor time series of seven spectral indices was derived from cross-calibrated Landsat program Surface Reflectance products via Google Earth Engine. Spectral indices were then combined with climatic variables (precipitation, reference evapotranspiration, air temperature) and then integrated in a statistical framework including Mann–Kendall test, Pettitt test, and Principal Component Analysis. Significant breakpoints were identified for the water fraction (2007; mean decrease from 0.18 to 0.09) and the Normalized Difference Vegetation Index (2009; mean increase from 0.30 to 0.42), consistent with a hydrological regime shift following the interruption of anthropogenic pressures. The relationship between the water fraction and the Vegetation Soil Salinity Index was 2.7 times steeper in the last period than the first one, indicating that, for an equivalent flooded extent, osmotic stress on vegetation is substantially higher under the artesian flow alone, likely due to reduced dilution of saline inputs combined with the effect of ongoing climate change. PCA showed that PC1 reflected the transition from anthropogenic to more natural system conditions, whereas PC2 was associated with increasing ET₀, became more prominent during the last period of management, suggesting a shift toward stronger climate-driven control. Long-term satellite monitoring provides a quantitative baseline for designing targeted management interventions aimed at sustaining ecosystem functioning under ongoing Mediterranean warming. Full article

Studies have shown that oceanic surface-water salinity varies across the globe and changes over time, while atmospheric water-vapor levels have also increased in recent decades. Evaporation from ocean and inland waters is controlled primarily by meteorological forcing, but the thermodynamic state of the water body also matters. In saline waters, dissolved solutes reduce water activity and thereby reduce the equilibrium tendency of water molecules to enter the vapor phase. In this study, the authors’ coefficient-less aqueous osmotic potential equation was used to examine the thermodynamic effect of representative oceanic salinity differences on evaporative tendency. Calculations were made for recorded surface-water salinities ranging from 31 to 38 kg·m⁻³ of dissolved solutes at an average temperature of 20 °C. Computed osmotic potentials ranged from −2.257 to −2.708 MPa. The corresponding semi-permeable membrane interface pressures ranged from 8.935 to 8.484 MPa, indicating an approximately 5% difference across the selected oceanic salinity range. The interface pressure calculated for solute-free water (11.192 MPa) was more than 24% higher than for the seawater cases considered. These results suggest that salinity acts as a secondary thermodynamic modifier of evaporation potential, whereas radiative, aerodynamic, humidity, and temperature controls remain dominant in determining actual evaporation fluxes. The results also indicate that freshwater bodies and changing land-based evaporative sources may contribute differently to atmospheric water vapor than saline ocean waters. The framework presented here is intended to complement, rather than replace, established evaporation formulations by clarifying how salinity-related osmotic effects can modify the water-side boundary condition. Full article

Conductive core–shell superabsorbent polymers (SAPs) are emerging as multifunctional additives for cementitious materials, combining moisture management with electrical functionality. In cement-based systems, a swellable polymeric core enables internal curing and crack-sealing through controlled water uptake and release, while a conductive shell introduces ionic and/or electronic charge transport, addressing key limitations of conventional non-conductive SAPs. This dual functionality provides a pathway toward smart cementitious composites with enhanced durability, self-sensing capability, and moisture-responsive behavior. This review focuses on the physical chemistry mechanisms governing conductive core–shell SAPs in cementitious environments, with emphasis on swelling thermodynamics, water transport kinetics, interfacial phenomena, and charge transport mechanisms. The roles of osmotic pressure, elastic network constraints, ionic effects, and pore solution chemistry are critically discussed, together with their impact on conductivity, hydration processes, microstructure development, and long-term performance. The relative contributions of ionic and electronic conduction are examined in relation to hydration state, shell morphology, and percolation of conductive networks. In addition, the relevance of core–shell SAP architectures to sustainable packaging is briefly discussed as a secondary application, illustrating how similar physicochemical principles—such as moisture buffering and functional coatings—apply beyond construction materials. Finally, key knowledge gaps are identified, including long-term stability in highly alkaline environments, trade-offs between swelling capacity and conductivity, environmental impacts of conductive phases, and the need for integrated experimental and modeling approaches. Addressing these challenges is essential for the rational design and practical implementation of conductive core–shell SAPs in next-generation cementitious materials. Full article

Gas condensate banking can significantly reduce near-well gas productivity by as much as ~60% in tight gas reservoirs. Existing treatment techniques are resource demanding and could alter the reservoir structure permanently. This study investigates the effectiveness of enhanced electrokinetic oil recovery (EK-EOR) as a low-impact alternative for treating condensate banks. Using compositional reservoir simulation (CMG GEM), the influence of key reservoir and operational parameters—porosity, permeability, producer well location (i, j), injection rate, and injection pressure—on cumulative gas production (CGP) was examined. A Box–Behnken design of experiments was employed to generate 62 simulation runs, and a proxy model was developed to approximate full-field responses. Statistical validation showed strong model fidelity (R² = 0.99, AAPE = 2.2%). The proxy was then optimized using a genetic algorithm (GA) to identify conditions that maximize gas recovery. Results indicate that lower injection rates and lower injection pressures maximize CGP through enhanced electro-osmotic flow and reduced water blocking, achieving a peak cumulative gas of 4.06 × 10⁸ ft³. A secondary optimum at high injection pressure could be attributed to re-pressurization and partial re-vaporization of condensate near the wellbore. Reservoir quality also exerted a strong control: higher permeability and moderate porosity favoured gas yield, while optimal producer placement near the reservoir boundary increased drainage efficiency. This study demonstrates a systematic optimization framework combining design of experiments, proxy modelling, and evolutionary algorithms to evaluate EK-EOR performance. Full article

Transforming growth factor-beta 1 (TGF-β1)-stimulated clone 22 domain (TSC22D) family genes (including TSC22D1-TSC22D4) were identified as transcription factors. It has been demonstrated that they display multiple functions due to proteins’ isoforms, redundancy, and other factors. Formerly, researchers mainly focused on its functions, like controlling cell growth and development, cell apoptosis, and balance of osmotic pressure in vivo. Nowadays, growing evidence indicates that they also play an important role in metabolic regulation and the immune system and are expected to be a new potential target for the treatment of diabetes or obesity. Despite this, it has been shown that TSC22D family genes have an inhibitory effect in multiple tumors. In this review, we significantly synthesized advances in metabolism, showing that TSC22D3 could control lipid accumulation via modulating adipogenesis and adipose differentiation, while TSC22D4 could regulate insulin sensitivity and gluconeogenesis by affecting Akt (serine/threonine kinase, also known as protein kinase B, or PKB) phosphorylation. Moreover, we provide novel insights, including the fact that TSC22D family genes function as a double-edged sword in cancer due to the type of tumor and tumor microenvironment (TME). Full article

Salt stress causes harm to plants through multiple aspects, such as osmotic pressure, ion poisoning, nutrient imbalance, and oxidative damage. Zinc finger proteins harboring two B-box domains, known as double B-box (DBB) proteins, constitute the DBB family. While DBB genes have been implicated in regulating circadian rhythms and stress responses in various plant species, their functions in cotton remain largely unexplored. The present study characterized the DBB gene family across the genomes of Gossypium hirsutum L., Gossypium raimondii L., and Gossypium arboreum L., revealing a complement of 58 members. These DBB genes were assigned to three separate clades based on phylogenetic analysis. Members possessing close phylogenetic relationships have similar conserved protein motifs and gene structures. All DBB proteins were predicted to be nuclear-localized, consistent with their roles as transcription factors. Furthermore, the presence of multiple cis-acting elements related to light, hormone, and stress responses in the promoters implies that GhDBBs are integral to cotton’s environmental stress adaptation. Expression pattern analysis indicated that the expression of GhDBB genes was associated with the plant’s response to multiple abiotic stresses, such as salt, drought, heat (37 °C), and cold (4 °C). The reliability of the expression data was confirmed by qPCR analysis of eight selected GhDBBs. Under 200 mM NaCl, Arabidopsis plants overexpressing GhDBB22 displayed longer roots and healthier true leaves than the wild-type controls. Conversely, VIGS-mediated silencing of GhDBB22 in G. hirsutum led to significantly reduced salt tolerance, accompanied by exacerbated oxidative damage. Taken together, the findings from our integrated genomic and functional analyses provide a foundational understanding of the molecular mechanisms through which proteins encoded by DBB genes are involved in the plant’s response to salt stress. Full article

Industrial whey dewatering via membrane processes remains challenging due to the rapid increase in viscosity, strong fouling tendencies from proteins and minerals, and the steep rise in osmotic pressure during concentration. These effects restrict operating windows and complicate energy-efficient process control. This study addresses the application of forward osmosis (FO) technology for industrial-scale dewatering of sweet whey using an Aquaporin Inside^® HFFO14 module. Various feed- and draw-side cross flow velocities (0.0397 to 0.0524 m s⁻¹ and 0.0127 to 0.0190 m s⁻¹, respectively) and draw solution (DS) osmotic pressures of 20 bar and 60 bar were investigated using a production-scale prototype plant. Sweet whey had an initial osmotic pressure of 7 bar and an electrical conductivity of 5.7 mS cm⁻¹. DS pressures of 20 bar and 60 bar resulted in a total recovery of 50% and over 80%, respectively. Water flux rates initially ranged from 10.1 to 11.6 L m⁻² h⁻¹ (LMH) and ceased at 3.3 LMH. Specific energy demand ranged from 0.15 to 1.1 kWh m⁻³. These findings support the feasibility of industrial-scale FO technology and underscore the potential of FO as an energy-efficient, sustainable solution for the dairy industry. However, frequent rinsing and cleaning routines are crucial to maintain membrane performance. Full article

Staphylococcus aureus is an important pathogen that can cause widespread infections as well as severe outbreaks of food poisoning. Recent studies have drawn attention to foodborne pathogens such as S. aureus endowed with the ability to form biofilms and increase resistance to antimicrobial agents as well as environmental stress, posing challenges to food safety. The Clp (caseinolytic protease) protein complex plays a crucial role in energy-dependent protein hydrolysis processes. This mechanism is a common way to maintain intracellular homeostasis and regulation in both prokaryotic and eukaryotic cells, especially under stress conditions. In S. aureus, multiple genes encoding Clp ATPase homologues have been identified: clpC, clpB, clpY, clpX, and clpL. This study investigated the roles of clpC in stress tolerance and biofilm formation of foodborne S. aureus RMSA24 isolated from raw milk. Our results showed that the deletion of the clpC gene significantly reduced the bacterium’s tolerance to heat, desiccation, hydrogen peroxide, and high osmotic pressure compared to wild type (WT). Furthermore, the clpC knockout mutant also exhibited a marked decrease in biofilm formation using Crystal Violet Staining (CVS) and Scanning Electron Microscopy (SEM). Finally, compared to WT, there was a total of 102 DEGs (differentially expressed genes), with a significant downregulation of genes related to biofilm formation (isaA and spa) and heat-shock response (clpP and danJ). These findings suggest that clpC regulates environmental tolerance in S. aureus by modulating the expression of stress- and biofilm-related genes, positioning it as a potential biomarker and a novel target for controlling contamination in the food industry. Full article

The blood–brain barrier (BBB) restricts the entry of therapeutic agents into the brain cardiovascular regulatory region, potentially contributing to drug-resistant hypertension. Objective: The objective of this study was to overcome this limitation by modifying PEGylated liposomes with transferrin (Tf) to facilitate Tf receptor binding at the BBB and penetratin (Pen), a cell-penetrating peptide, to enhance neuronal uptake. Methods: This study evaluated the efficacy of Tf-Pen-liposomes in delivering angiotensin-converting enzyme 2 (ACE2) or EGFP (control) genes across the BBB in rats. In addition, the therapeutic effect of intravenous administration of Tf-Pen-Lip carrying plasmid DNA encoding ACE2 (Tf-Pen-Lip-pACE2) was tested in a neurogenic hypertension model induced by intracerebroventricular (ICV) infusion of angiotensin II (Ang II) via osmotic pump implantation and brain cannulation. Results: Conjugation with Tf and Pen significantly enhanced liposome-mediated gene transfection in cultured cells and increased transport across an in vitro BBB model. In vivo, intravenous administration of Tf-Pen-Lip-pACE2 or Tf-Pen-Lip-pGFP successfully elevated ACE2 or EGFP expression, respectively, in the hypothalamic paraventricular nucleus (PVN). Chronic ICV infusion of Ang II produced a sustained increase in blood pressure and heart rate, accompanied by sympathetic overactivation and elevated arginine vasopressin (AVP) secretion, hallmarks of neurogenic hypertension. Notably, intravenous Tf-Pen-Lip-pACE2 treatment dramatically attenuated Ang II–induced neurogenic hypertension, whereas Tf-Pen-Lip-pGFP had no effect on pressor responses, sympathetic activity, or AVP secretion. Conclusions: This dual-functionalized liposomal delivery system effectively transported the ACE2 gene across the BBB into the brain, increased ACE2 expression, and markedly attenuated neurogenic hypertension following systemic administration. Full article

Hypertension is a major risk factor for heart failure. Acetylation of p53 is known to regulate its activities. We have previously identified that p53 acetylation is required for cardiac remodeling in a mouse model of pressure overload-induced heart failure. Acetylation mutant p53 (p53aceKO) mice have been shown to have the ability to regulate SIRT3 KO-induced cardiac fibrosis. In the present study, we hypothesized that p53aceKO mice would exhibit cardiac protection and blunt cardiac fibrosis when subjected to Ang-II-induced hypertension. Control and p53aceKO mice received either a micro-osmotic pump implant administering Ang-II for 28 days or a sham procedure. Blood pressure was measured weekly, and echocardiography was performed every two weeks. Mice were euthanized and hearts were processed for histological analysis. While both control and p53aceKO mice receiving Ang-II exhibit increased systolic and diastolic blood pressures, control mice also demonstrate increases in ejection fraction and fractional shortening compared to the sham, while p53aceKO mice do not. Furthermore, control mice receiving Ang-II exhibit decreased left ventricular diameter and volume at end-systole and end-diastole, as well as thickening of both the anterior and posterior walls, while p53aceKO mice exhibit no significant changes in any of these parameters. Additionally, p53aceKO mice do not exhibit the Ang-II infusion-induced cardiac fibrosis seen in control mice treated with Ang-II. Mutation of p53 acetylation is protective against Ang-II infusion-induced cardiac fibrosis and dysfunction in mice. Acetylated p53 may, therefore, be a novel therapeutic target to address complications in the heart associated with hypertension. Full article