Search Results (557)

Enhancing crop diversity is a key pillar of the EU’s 2023–2027 CAP eco-schemes, yet Poland’s long-standing crop simplification raises doubts about the policy’s effectiveness. This study assesses the determinants of crop structure and crop diversity in Poland and evaluates whether eco-schemes generate measurable improvements at farm and municipal scales. A multilevel approach integrates municipality-level saturation with ecological interventions (eco-schemes, organic farming, and agri-environment–climate measures) with longitudinal data from 192 purposively selected farms across all 16 voivodeships for 2016, 2021, and 2024. Crop diversity is measured using the Shannon–Wiener index (H′), combined with indicators of specialization, farm size, and participation in CAP instruments. Spatial and temporal comparisons reveal that farms engaged in commercial animal production maintain simplified crop structures, which is associated with specialization as a dominant factor in low diversity. Maize share increased steadily from 12.4% to 16.7%, signalling ongoing homogenization, particularly in livestock-intensive regions. Contrary to earlier assumptions, smaller farms did not exhibit higher diversity, suggesting organizational constraints. At the municipal level, greater saturation with eco-schemes and organic farming was associated with higher H′ values, while areas with strong agri-environment–climate presence—often mountainous—showed lower diversity due to biophysical limits. The findings highlight structural barriers that may limit eco-scheme impacts. Full article

Livestock and poultry manure is an important recyclable nutrient resource in Chinese agriculture, and heap composting, a low-input static method, is the most common treatment approach on farms. However, most studies have focused on aerobic composting, whereas systematic evaluations of physicochemical evolution and maturity/quality development during heap composting remain limited, hampering reliable assessment of compost performance and land-application readiness. Here, we compared heap and turned composting of chicken manure amended with rice bran under natural aeration. Five treatments were applied: manure alone (CM), manure with rice bran (CM+RB), covered heap compost (CM+RB+C), single-turned compost (CM+RB+ST), and multi-turned compost (CM+RB+MT), monitored for 66 days. Rice-bran addition rapidly induced the thermophilic phase and substantially enhanced organic decomposition, while turning further prolonged the thermophilic phase. Humic acid content increased in all rice-bran treatments, indicating clear humification, with only slight variation among aeration intensities. Nitrogen transformation also differed: turned piles showed faster nitrification, suggesting enhanced aerobic nitrogen conversion under stronger aeration. Compost maturity improved across treatments, and all rice-bran treatments except CM+RB+C achieved a germination index > 70%. Overall, heap composting largely achieved stability, humification, and maturity close to those of aerobic turning, while markedly reducing labor and energy inputs, supporting its suitability for small-scale manure recycling. Full article

The quality of cow’s milk is critical for human nutrition; thus, it is important to develop rapid, sensitive, and cost-effective methods to monitor milk quality. Volatile Organic Compounds (VOCs) from milk are odorant molecules that can be used as key indicators of milk quality, since their presence is influenced by important factors such as animal metabolism, animal diet, and farming practices. In this work, we used the porcine odorant-binding protein (pOBP) and the bovine odorant-binding protein (bOBP) as molecular recognition elements (MREs) of an innovative fluorescence biosensor to detect the presence of odorant molecules in (a) milk produced by intensive livestock farming and (b) milk produced by extensive livestock farming. For biosensors, it is important to use proteins that are stable under operative conditions; therefore, we used fluorescence spectroscopy for a biophysical characterization of the pOBP and of the bOBP at different temperatures. The proposed biosensor employs a system to capture the odorant molecules from milk, which are then transferred to a liquid phase for quantitative and qualitative analyses. The binding of the odorant molecules to the OBPs triggers a Förster Resonance Energy Transfer (FRET) signal, allowing for real-time VOC quantification. The performance of the assays was evaluated by Headspace Solid-Phase Microextraction coupled with Gas Chromatography–Mass Spectrometry (HS-SPME/GC-MS) experiments. The experimental approach used for the development of the biosensor demonstrated high sensitivity and specificity, enabling the differentiation of milk from intensive and extensive farming systems. The results indicate the potential of this method for the real-time monitoring of VOCs in milk samples for food traceability and quality control. Full article

This collection presents the accepted abstracts for the 3rd International Online Conference on Agriculture (IOCAG2025), organized by the MDPI journal Agriculture, and held online from 22 to 24 October 2025. The event highlighted integrative solutions and data-driven innovations for building resilient agricultural systems, spanning topics from climate-smart practices, precision water management, and AI-enhanced smart farming to sustainable crop protection, soil health, and advanced crop genetics. Discussions emphasized translating research into practical applications that balance productivity with environmental stewardship. Collectively, IOCAG2025 showcased forward-looking approaches and collaborative insights aimed at advancing sustainable, efficient, and climate-adapted agriculture on a global scale. Full article

Unmanned aerial vehicle (UAV)-based multispectral monitoring has become an increasingly important tool for assessing crop vigor and stress under commercial agricultural conditions. However, most UAV-based studies using the normalized difference vegetation index (NDVI) in citrus systems have focused on yield estimation, disease detection, or canopy characterization during active growth phases, while the immediate post-harvest recovery period remains poorly documented. In this study, UAV-derived NDVI products were used to evaluate the canopy response in a commercial ‘Washington Navel’ orange orchard located in La Yarada Los Palos district (Tacna, Peru) following harvest. The study specifically assessed the effect of an on-farm, residue-based organic biostimulant produced from local organic wastes within a circular economy framework. The results indicate that treated plots exhibited a faster and more pronounced recovery of canopy vigor compared to untreated controls during the early post-harvest period. By integrating high-resolution UAV-based multispectral monitoring with a residue-derived biostimulant strategy, this work advances current NDVI-based applications in citrus by shifting the analytical focus from productive stages to post-harvest physiological recovery. The proposed approach provides a scalable and non-invasive framework for evaluating post-harvest canopy dynamics under water-limited, hyper-arid conditions and highlights the potential of locally sourced biostimulants as complementary management tools in precision agriculture systems. Full article

Seven disease control products produced at a grapevine farm in Mała Wieś (Masovian County, Poland) were tested and compared with standard chemical control and no control. The following substances were used as disease control agents: lavender, lemon, and orange oils; Saccharomyces cerevisiae HDT18 yeast fermentation liquid; mint; and Habanero Orange hot pepper decoction. Results were compared with treatments using standard fungicides, a control without control, and two commercial products containing tansy extract and chili and garlic extract. The tested products were generally less effective in protecting against fungal diseases than the standard chemical control. However, they improved total yield, yield used for vinification, total acidity, sugar content, and pH of harvested grapes compared to untreated grapes. Our own preparations (HDT-18, lemon oil, mint decoction, and hot pepper) reduced total yields and simultaneously reduced the number of grapes infected with diseases, which is particularly important in mechanical harvesting without sorting. Must parameters (sugar, pH, TA) enabled wine production from each of the experimental combinations tested. These approaches may be useful in organic farming programs in cold climates. Although they may reduce total yields, they also reduce the number of grapes unsuitable for vinification. Full article

Salinity stress represents a critical environmental constraint that significantly limits the development of tilapia aquaculture in brackish water environments. Its substantial impacts on fundamental physiological processes in fish, particularly osmotic balance, energy metabolism, and antioxidant defense mechanisms, have become a major scientific concern in aquaculture research. To systematically elucidate the molecular mechanisms underlying the response of genetically improved farmed tilapia (Oreochromis niloticus) to salinity stress and to test the hypothesis that it adapts through metabolic reprogramming for energy reallocation under such conditions, this study employed an integrated transcriptomic and metabolomic approach. Through a rigorously controlled experimental design with freshwater (0‰) as the control group and brackish water (24‰) as the experimental group, we conducted a comprehensive analysis of dynamic changes in gene expression profiles and metabolite spectra in the liver tissues of experimental fish. The study yielded the following key findings: First, salinity stress significantly suppressed growth performance indicators, including body weight and length, while simultaneously inducing extensive transcriptomic restructuring and profound metabolic remodeling in liver tissue. A total of 1529 differentially expressed genes (including 399 up-regulated and 1130 down-regulated genes) and 127 significantly differential metabolites were identified. Second, the organism achieved strategic reallocation of energy resources through coordinated suppression of multiple energy-consuming anabolic pathways, particularly steroid biosynthesis and fatty acid metabolism, with the remarkable down-regulation of Fasn, a key gene in the fatty acid synthesis pathway, being especially prominent. Energy-sensing and metabolic homeostasis regulatory networks played a central coordinating role in this process, guiding the organism through metabolic reprogramming by regulating downstream metabolic nodes. From a multi-omics integrative perspective, this study provides in-depth insights into the sophisticated metabolic remodeling and energy allocation strategies employed by GIFT to cope with salinity stress. These findings, particularly the suppression of fatty acid biosynthesis and the reprogramming of glycolysis/gluconeogenesis pathways, not only elucidate the molecular mechanisms by which teleosts achieve environmental adaptation through energy reallocation, but also provide actionable molecular targets for the selective breeding of salinity-resilient tilapia strains. Full article

Smallholder farmers in semi-arid regions worldwide face persistent water scarcity, declining soil fertility, and increasing climate variability, which constrain food production. This study investigated soil and water management practices and their effects on soil health, crop productivity, and adoption among smallholder vegetable farmers in a semi-arid area in Kenya. A mixed-methods approach was employed, combining survey data from 397 farmers with a randomized field experiment. Results showed that hand watering (88.7%) and manure application (95.5%) were prevalent, while only 5.7% of farmers used drip irrigation. Compost and mulch treatments significantly improved soil organic carbon (p = 0.03), available water capacity (p = 0.01), and gravimetric moisture content (p = 0.02), with soil moisture conservation practices strongly correlated with higher yields in leafy green vegetables (R = 0.62). Despite these benefits, adoption was hindered by high water costs (42.6%) and unreliable sources (25.7%). Encouragingly, 96.2% of respondents expressed willingness to pay for improved water systems if affordable and dependable. The findings stress the need for integrated water–soil strategies supported by inclusive policy, infrastructure investment, and gender-responsive training to enhance resilience and productivity in smallholder farming under water-scarce conditions across sub-Saharan Africa and other regions globally, contributing to global sustainability targets such as SDG 6, 12 and 15. Full article

Campylobacter is a leading foodborne bacterial pathogen, and poultry production is a major reservoir contributing to human exposure. Reducing Campylobacter at farm level is therefore critical to limit downstream contamination. This systematic review and meta-analysis aimed to identify and quantitively summarise the current interventions and control measures applied in poultry farms to control the contamination and bird colonisation by Campylobacter. The Scopus electronic database was accessed to collect primary research articles that focused on observational studies and in vivo experiments, reporting results on Campylobacter concentrations or prevalence in both non-intervened and intervened groups. A total of 4080 studies were reviewed, from which 112 were selected and included in the meta-analysis according to predefined criteria, yielding 1467 observations. Meta-regression models were adjusted to the full data set and by intervention strategy based on the type of outcome measure (i.e., concentration and prevalence). In general terms, the results reveal that the effectiveness to reduce Campylobacter colonisation vary among interventions. A highly significant effect (p < 0.001) was observed in interventions such as organic acids, bacteriophages, plant extracts, probiotics, and organic iron complexes added to feed or drinking water; although drinking water was proven to be a more effective means of administration than feed for extracts and organic acids. In contrast, interventions such as chemical treatments, routine cleaning and disinfection, and vaccination showed both lower and more heterogeneous effects on Campylobacter loads. Vaccination effects were demonstrated to be driven by route and schedule, with intramuscular administration, longer vaccination periods and sufficient time before slaughter linked to greater reduction in Campylobacter colonisation. Probiotics, plant extracts and routine cleaning and disinfection were associated with lower Campylobacter prevalence in flocks. Meta-regression models consistently showed that the interventions were proven more effective when the sample analysed was caecal contents in comparison to faeces (p < 0.001). Overall, the findings of this meta-analysis study emphasise the application of a multi-barrier approach that combines targeted interventions with robust biosecurity and hygiene measures in order to reduce Campylobacter levels in poultry farms. Full article

The aim of this research is to elaborate on the activities of non-formal experiential learning in agritourism developed by an organic family farm in Thessaloniki, Greece. Based on a qualitative approach, in-depth interviews with the owners of the farm and a Business Model Canvas (BMC) approach to this case study, the results indicate that workshops, seminars on nutrition, environmental conservation activities, plant identification, hands-on activities for children and cooking lessons with chefs are some of the non-formal learning tools. The aforementioned activities, on the one hand, raise gate sales for the family and, on the other hand, promote knowledge and awareness towards the contemporary environmental challenges that the rural areas and our food chain are facing. Future development strategies were also identified through the BMC, such as the adoption of digital educational tools, and ‘Do It Yourself’ kits for growing microgreens at home, while the need for official certification and support of multifunctional farms by the Ministry of Rural Development was also highlighted. Full article

Antimicrobial resistance (AMR) represents a significant global concern, undermining the efficacy of treatments in both human and veterinary medicine. Livestock production plays a major role in the emergence and dissemination of AMR, primarily due to the extensive use of antibiotics for therapeutic, prophylactic, and metaphylactic purposes. Addressing this multifaceted issue necessitates a One Health approach. At the international level, regulatory frameworks are predominantly non-binding, relying on soft-law instruments developed by the World Health Organization (WHO), the Food and Agriculture Organization (FAO), and the World Organization for Animal Health (WOAH, formerly OIE), which advocate for harmonized guidelines and national action plans. In contrast, the European Union has implemented binding regulations, including Regulation (EU) 2019/6 and Regulation (EU) 2019/4, which restrict non-essential antimicrobial use (AMU) and reinforce veterinary accountability. Initiatives such as the Farm to Fork Strategy and platforms like ClassyFarm further advance antimicrobial stewardship by integrating animal welfare, sustainability, and access to EU funding. Achieving substantial reductions in AMR within livestock systems requires coordinated, cross-disciplinary, and multi-level governance efforts. The EU model illustrates how enforceable legal frameworks, combined with science-based monitoring and welfare incentives, can facilitate prudent antibiotic use and promote sustainable animal production. This review aims to provide an integrated overview of international and European strategies for regulating antibiotic use in food-producing animals, focusing on how scientific, veterinary and legal perspectives contribute to combating AMR and promoting animal welfare by emphasizing prevention, and a prudent and responsible AMU. Full article

Aquatic environments have been a critical source of nutrition for millennia, with wild fisheries supplying protein and nutrients to populations worldwide. A notable shift has occurred in recent decades with the expansion of aquaculture, now representing a fast-growing sector in food production. Aquaculture plays a key role in mitigating the depletion of wild fish stocks and addressing issues related to overfishing. Despite its potential benefits, the sustainability of both wild and farmed aquatic food systems is challenged by anthropogenic pollution. Contaminants from agricultural runoff, industrial discharges, and domestic effluents enter freshwater systems and eventually reach marine environments, where they may be transported globally through ocean currents. Maintaining water quality is paramount to food safety, environmental integrity, and long-term food security. In addition to conventional seafood products such as fish and shellfish, foods such as those derived from microalgae are gaining attention in Western markets for their high nutritional value and potential functional properties. These organisms have been consumed in Asia for generations and are now being explored as sustainable foods and ingredients as an alternative source of protein. Contaminants in aquatic food products include residues of agrochemicals, persistent organic pollutants (POPs) such as dioxins, polychlorinated biphenyls (PCBs), and per- and polyfluoroalkyl substances (PFASs), as well as brominated flame retardants and heavy metals. Public and scientific attention has intensified around plastic pollution, particularly microplastics and nanoplastics, which are increasingly detected in aquatic organisms and are the subject of ongoing toxicological and ecological risk assessments. While the presence of these hazards necessitates robust risk assessment and regulatory oversight, it is important to balance these concerns against the health benefits of aquatic foods, which are rich in omega-3 fatty acids, high-quality proteins, vitamins, and trace elements. Furthermore, beyond direct human health implications, the environmental impact of pollutant sources must be addressed through integrated management approaches to ensure the long-term sustainability of aquatic ecosystems and the food systems they support. This review covers regulatory frameworks, risk assessments, and management issues relating to aquatic environments, including the impact of climate change. It aims to serve as a comprehensive resource for researchers, policymakers, food businesses who harvest food from aquatic systems and other stakeholders. Full article

Bread wheat variety development suited to organic farming conditions remains a major challenge mainly because of the high breeding costs involved and the few cultivars adapted to low-input systems. The present work explores whether early generation selection needs to take place under organic conditions for subsequent adaptation or whether conventional testing at an early stage could be adequate. A diverse set of crosses involving Greek landraces and commercial cultivars were developed and advanced by honeycomb pedigree selection under both organic and conventional environments. Subsequently, F₄ progenies and an upgraded landrace were evaluated over two years in neighboring organic and conventional trials. Both statistical and GGE biplot analyses revealed significant genotype × environment interactions. The results clearly indicate that early selection under organic conditions did not provide a consistent advantage for subsequent performance under organic management compared with conventional early selection. Genotypes derived from the Africa × Atheras cross consistently showed the highest and most stable yields across the two environments, irrespective of the early selection environment. These results indicate that genetic background and landrace-derived diversity are more important than the early selection environment for the expression of performance. A staged breeding strategy involving initial selection in conventional management followed by multi-environment testing in organic conditions can provide a cost-effective approach to developing resilient, high-yielding wheat cultivars suitable for organic farming systems, which are typically characterized by low-input management practices, and in tune with the EU targets for expanded organic farming. Full article

Modern agricultural operations generate high-volume and diverse data (historical and stream) from various sources, including IoT devices, robots, and drones. This paper presents a novel smart farming architecture specifically designed to efficiently manage and process this complex data landscape.The proposed architecture comprises five distinct, interconnected layers: The Source Layer, the Ingestion Layer, the Batch Layer, the Speed Layer, and the Governance Layer. The Source Layer serves as the unified entry point, accommodating structured, spatial, and image data from sensors, Drones, and ROS-equipped robots. The Ingestion Layer uses a hybrid fog/cloud architecture with Kafka for real-time streams and for batch processing of historical data. Data is then segregated for processing: The cloud-deployed Batch Layer employs a Hadoop cluster, Spark, Hive, and Drill for large-scale historical analysis, while the Speed Layer utilizes Geoflink and PostGIS for low-latency, real-time geovisualization. Finally, the Governance Layer guarantees data quality, lineage, and organization across all components using Open Metadata. This layered, hybrid approach provides a scalable and resilient framework capable of transforming raw agricultural data into timely, actionable insights, addressing the critical need for advanced data management in smart farming. Full article

In northwestern Ecuador, where more than 90% of the original forest cover has been lost, it is unknown how soil chemistry influences bat diversity. This study evaluated bat diversity, non-herbaceous plant community structure, and soil nutrients in 30 plots distributed across crops on two farms separated by 32 km. Soil analyses revealed variations in organic matter and nutrients, identifying calcium, magnesium, zinc and iron as the most influential. A total of 1662 individuals of 24 non-herbaceous plant species and 193 individuals of 16 bat species were recorded, dominated by frugivorous and nectarivorous guilds. Generalized linear mixed models showed significant relationships between bat diversity indices and soil nutrients. These elements improve tree growth, fruiting, and flowering, which increases the quality and availability of food resources for bats. In return, these mammals provide key ecosystem services such as pollination, seed dispersal, and insect control. Our findings highlight that soil chemistry indirectly regulates bat communities by influencing vegetation structure and resource availability. This integrated approach underscores the importance of soil–plant–animal interactions in tropical agricultural landscapes, offering practical guidance. Full article