Search Results (173)

This work explores the application of robotic systems in archaeology, highlighting their transformative role in excavation, documentation, and the preservation of cultural heritage. By combining technologies such as LiDAR, GIS, 3D modeling, sonar, and other sensors with autonomous and semi-autonomous platforms, archaeologists can now reach inaccessible sites, automate artifact analysis, and reconstruct fragmented remains with greater precision. The study provides a systematic overview of underwater, aerial, terrestrial, and other robotic systems, drawing on scientific literature that showcases their innovative use in both fieldwork and museum settings. Selected examples illustrate how robotics is being applied to solve key archaeological challenges in new and effective ways. While the paper emphasizes the potential of these technologies, it also addresses their technical, economic, and ethical limitations, concluding that successful adoption depends on interdisciplinary collaboration, careful implementation, and a balanced respect for cultural integrity. Full article

Microneedle arrays (MNAs) are becoming increasingly popular due to their ease of use and effectiveness in drug delivery and diagnostic applications. Improvements in three-dimensional (3D) printing techniques have made it possible to fabricate MNAs with high precision, intricate designs, and customizable properties, expanding their potential in medical applications. While most studies have focused on transdermal applications, non-transdermal uses remain relatively underexplored. This review summarizes recent developments in 3D-printed MNAs intended for non-transdermal drug delivery and diagnostic purposes. It includes a literature review of studies published in the past ten years, organized by the target delivery site—such as the brain and central nervous system (CNS), oral cavity, eyes, gastrointestinal (GI) tract, and cardiovascular and reproductive systems, among other emerging areas. The findings show that 3D-printed MNAs are more adaptable than skin-based delivery, opening up exciting new possibilities for use in a variety of organs and systems. To guarantee the effective incorporation of polymeric non-transdermal MNAs into clinical practice, additional research is necessary to address current issues with materials, manufacturing processes, and regulatory approval. Full article

Accurate analysis of medical videos remains a major challenge in deep learning (DL) due to the need for effective spatiotemporal feature mapping that captures both spatial detail and temporal dynamics. Despite advances in DL, most existing models in medical AI focus on static images, overlooking critical temporal cues present in video data. To bridge this gap, a novel DL-based framework is proposed for spatiotemporal feature extraction from medical video sequences. As a feasibility use case, this study focuses on gastrointestinal (GI) endoscopic video classification. A 3D convolutional neural network (CNN) is developed to classify upper and lower GI endoscopic videos using the hyperKvasir dataset, which contains 314 lower and 60 upper GI videos. To address data imbalance, 60 matched pairs of videos are randomly selected across 20 experimental runs. Videos are resized to 224 × 224, and the 3D CNN captures spatiotemporal information. A 3D version of the parallel spatial and channel squeeze-and-excitation (P-scSE) is implemented, and a new block called the residual with parallel attention (RPA) block is proposed by combining P-scSE3D with a residual block. To reduce computational complexity, a (2 + 1)D convolution is used in place of full 3D convolution. The model achieves an average accuracy of 0.933, precision of 0.932, recall of 0.944, F1-score of 0.935, and AUC of 0.933. It is also observed that the integration of P-scSE3D increased the F1-score by 7%. This preliminary work opens avenues for exploring various GI endoscopic video-based prospective studies. Full article

Rheum tataricum L.fil., known for its high tolerance to drought, salinity, and nutritional deficiency, is the least studied species of wild rhubarb. Extract of roots and rhizomes of R. tataricum has been traditionally used for the treatment of different diseases such as liver, kidney, womb, and bladder diseases and also relapsing fever. An ethanol extract of the roots of R. tataricum was prepared and further successively fractionated by extraction with tert-butyl methyl ether (TBME) and ethyl acetate (EtOAc). The obtained extract fractions were subjected to a series of chromatographic separations on silica gel for the isolation of its individual compounds. A total of 12 individual compounds, 2-O-β-D-glucopyranoside of R-(4-hydroxyphenyl)-2-butanol (rhododendrin) 1, gallic acid 2, 2-O-β-D-glucopyranoside of S-4-(4-hydroxyphenyl)-2-butanol (epi-rhododendrin) 3, their aglycones (-)-(2R)-rhododendrol 4 and (+)-(2S)-rhododendrol 5, gallotannin β-glucogallin 6, chlorogenic acids (3,5-di-O-caffeoylquinic acid 7 and 5-O-caffeoyl-3-O-(p-coumaroyl) quinic acid 8), 4-(4-hydroxyphenyl)-2-butanon (raspberry ketone) 9 and three stilbenes (rhaponticin 10, desoxyrhaponticin 11 and resveratroloside 12), were isolated and characterized. The structure of desoxyrhaponticin 11 was confirmed by X-ray diffraction analyses. The results of in vitro biological assays (the MTT test) showed that ethanol extract Rheum tataricum was non-toxic against the normal epithelial VERO cells. The isolated compounds 1, 4, 11 and 12 exhibited cytotoxicity against a cervical cancer cell line (CaSki), breast adenocarcinoma (MCF7) and glioblastoma cell line (SNB-19) at low micromolar concentrations. Polyhydroxystilbenes 11 and 12 showed the best potency against adenocarcinoma cells (GI₅₀ = 7–8 μM). The inhibition activity towards cancer cells was comparable to those of the standard drug doxorubicin. The available from R. tataricum secondary metabolites may serve as new leads for the discovery of anticancer drugs. Full article

Background/Objectives: Bcr-Abl inhibitors such as imatinib have been used to treat chronic myeloid leukemia (CML). However, the efficacy of these drugs has diminished due to mutations in the kinase domain, notably the T315I mutation. Therefore, in this study, new purine derivatives were designed as Bcr-Abl inhibitors based on 3D-QSAR studies. Methods: A database of 58 purines that inhibit Bcr-Abl was used to construct 3D-QSAR models. Using chemical information from these models, a small group of new purines was designed, synthesized, and evaluated in Bcr-Abl. Viability assays were conducted on imatinib-sensitive CML cells (K562 and KCL22) and imatinib-resistant cells (KCL22-B8). In silico analyses were performed to confirm the results. Results: Seven purines were easily synthesized (7a–g). Compounds 7a and 7c demonstrated the highest inhibition activity on Bcr-Abl (IC₅₀ = 0.13 and 0.19 μM), surpassing the potency of imatinib (IC₅₀ = 0.33 μM). 7c exhibited the highest potency, with GI₅₀ = 0.30 μM on K562 cells and 1.54 μM on KCL22 cells. The GI₅₀ values obtained for non-neoplastic HEK293T cells indicated that 7c was less toxic than imatinib. Interestingly, KCL22-B8 cells (expressing Bcr-Abl^T315I) showed greater sensitivity to 7e and 7f than to imatinib (GI₅₀ = 13.80 and 15.43 vs. >20 μM, respectively). In silico analyses, including docking and molecular dynamics studies of Bcr-Abl^T315I, were conducted to elucidate the enhanced potency of 7e and 7f. Thus, this study provides in silico models to identify novel inhibitors that target a kinase of significance in CML. Full article

Unmanned systems provide significant prospects for improving the efficiency of electromagnetic geophysical exploration in mineral prospecting and geological mapping, as they can significantly increase the productivity of field surveys by accelerating the movement of the measuring system along the site, as well as minimizing problems in cases where the pedestrian walkability of the site is a challenge. Lightweight and cheap UAV systems with a take-off weight in the low tens of kilograms are unable to carry a powerful current source; therefore, semi-airborne systems with a ground transmitter (an ungrounded loop or grounded at the ends of the line) and a measuring system towed on a UAV are becoming more and more widespread. This paper presents the results for a new generation of semi-airborne technology SibGIS UAV-TEMs belonging to the “line-loop” type and capable of realizing the transient/time-domain (TEM) electromagnetics method used for studying a uranium object of the paleovalley type. Objects of this type are characterized by a low resistivity of the ore zone located in relatively high-resistivity host rocks and, from the position of the geoelectric structure, can be considered a good benchmark for assessing the capabilities of different electrical exploration technologies in general. The aeromobile part of the geophysical system created is implemented on the basis of a hexacopter carrying a measuring system with an inductive sensor, an analog of a 50 × 50 m loop, an 18-bit ADC with satellite synchronization, and a transmitter. The ground part consists of a galvanically grounded supply line and a current source with a transmitter creating multipolar pulses of quasi-DC current in the line. The survey is carried out with a terrain drape based on a satellite digital terrain model. The article presents the results obtained from the electromagnetic soundings in comparison with the reference (drilled) profile, convincingly proving the high efficiency of UAV-TEM. This approach to pre-processing UAV–electrospecting data is described with the aim of improving data quality by taking into account the movement and swaying of the measuring system’s sensor. On the basis of the real data obtained, the sensitivity of the created semi-airborne system was modeled by solving a direct problem in the class of 3D models, which allowed us to evaluate the effectiveness of the method in relation to other geological cases. Full article

Geographic Information System (GIS)-based noise management is crucial in urban environments as it provides precise spatial analysis, helping to identify noise hotspots and optimize noise control measures. By integrating noise propagation models with GIS technology, dynamic simulation and visualization of noise distribution can be achieved, offering scientific support for urban planning and noise management. Most existing noise prediction models fail to fully account for three-dimensional (3D) spatial information and a wide range of environmental factors. As a result, there are often discrepancies between the actual noise measurements at monitoring points and the predicted values generated by these models. Furthermore, there is a lack of a system that can effectively integrate noise data with three-dimensional scenes for simulation. This paper proposes a new method to simulate urban noise propagation, aiming to achieve more accurate noise prediction and visualization in a three-dimensional environment. First, we computed the preliminary noise propagation based on a traffic noise model. Next, machine learning techniques were applied to analyze the relationship between noise discrepancies and multi-modal factors, thereby improving the accuracy of environmental noise level estimation. Based on this, we developed an urban noise simulation system. The system integrates functions such as noise simulation, traffic simulation, and weather changes, enabling accurate noise visualization within a three-dimensional virtual environment. Experimental results demonstrate that this method enhances the accuracy of urban noise prediction and visualization, providing users with a more comprehensive understanding of the spatial distribution of urban noise. Full article

The integration of new technologies across scientific disciplines, including rock slope engineering, is increasingly essential. The use of drones and advanced software tools for mapping and analyzing large rocky slopes in detail has become indispensable and highly beneficial. In this context, this paper examines the process and key parameters involved in generating a high-resolution 3D terrain model and evaluating rock-mass stability using advanced software tools (UgCS version 5.5.0, ArcGIS version 3.4.3, Drone2Map version 2024.2.1, DSE version 3.02, and Rocfall3D version 1.014). These technologies facilitate the identification of hazard zones on rocky slopes in monuments of archaeological interest. The evaluation of this modeling approach is conducted at the monolithic rock of Ancient Corinth (Acrocorinth), one of Greece’s most significant archaeological sites. This study focuses on assessing its vulnerability to rockfalls and identifying hazard zones. This methodology involves the development of a 3D rockfall analysis system and the implementation of a specialized hazard matrix for the quantitative assessment of rockfall risk. This approach enables the development of a decisive model for mitigating rockfall hazards, ensuring the safety of visitors in high-traffic areas such as major archaeological sites. Full article

Background/Objectives: D4R antagonists have recently been suggested as potential therapeutic alternatives to the standard treatments of glioblastoma (GBM). In this study, new piperidine-based ligands, analogs of the potent and selective D4R compounds 77-LH-28-1 (7) and its 4-benzyl analog 8, were synthesized and studied to investigate the effects produced by variations in the distances between the pharmacophoric features on the D4R affinity and selectivity. Methods: All the new compounds 9–20 were evaluated for their radioligand binding affinity at D2-like receptor subtypes and the results were rationalized by docking studies and molecular dynamics (MD) simulations. The functional profiles of the most interesting derivatives were assessed at D4R Go and Gi protein and β-arrestin by BRET assay and their potential anticancer activity was determined in GBM cell lines. Results: Radioligand binding results highlighted that the derivatives bearing a terminal butyl chain showed structure–activity relationships different from those with a benzyl terminal. From functional studies performed on the best derivatives 12 and 16, the response profiles of both compounds were more robust in antagonist mode, with derivative 16 showing higher antagonist potency than 12 across all three transducers. Interestingly, 12 and 16 dose-dependently decreased the cell viability of GBM cells, inducing cell death and cell cycle arrest, promoting an increase in ROS production, causing mitochondrial dysfunction, and significantly inhibiting colony formation. Conclusions: The promising biological profiles of 12 and 16 make them new lead candidates that warrant further investigation to gain a better understanding of the mechanism behind their antitumor activity and better evaluate their potential for GBM treatment. Full article

Rabbit hemorrhagic disease virus (RHDV) is a very virulent virus of the genus Lagovirus causing severe and fatal hepatitis in the European rabbit (Oryctolagus cuniculus). RHDV has two distinct genotypes: GI.1 (RHDV) and GI.2 (RHDV2). The first RHDV2/GI.2 strain was identified as a recombinant virus between a non-pathogenic (GI.3P) and a pathogenic (GI.2) lagovirus, and the recombination is thought to have been a key mechanism in the emergence and evolution of RHDV2. Here, a new variant of RHDV2 was identified affecting domestic rabbits on Chinese farms, with a mortality rate of 70–80%. Phylogenetic analysis indicated that the nonstructural portion of this newly identified strain’s genome clustered with the GI.1a variants. In contrast, the capsid gene shared the highest nucleotide identity of 97.9% with the North American GI.2 strains, suggesting a possible introduction in China of North American strains and recombination with the GI.1a strains circulating in China. We have produced a recombinant vaccine using the first Chinese GI.2 strain, SC2020/0401, by cloning the vp60 gene into a baculovirus expression vector. Virus-like particles (VLPs) were then produced in Sf9 insect cells, and a challenge study was performed. Rabbits immunized with the VLP vaccine survived 7 d after being challenged with the new virus. The results indicate that commercial vaccines are urgently required in China to control the circulation of RHDV2 variants. Full article

Aquisinenins G–I (1–3), three new 2-(2-phenylethyl)chromone-sesquiterpene hybrids, were isolated from the ethanol extract of Hainan agarwood derived from Aquilaria sinensis. Spectroscopic techniques, such as ¹D and ²D NMR and HRESIMS, were used to determine their structures. Experimental and computed ECD data were compared to confirm their absolute configurations. Compounds 1–3 are uncommon dimeric derivatives of 2-(2-phenylethyl)chromone-sesquiterpene, characterized by the fusion of 5,6,7,8-tetrahydro-2-(2-phenylethyl)chromone with agarofuran or agarospirane-type sesquiterpene units by an ester linkage. Compound 1 inhibited nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells, showing an IC₅₀ value of 22.31 ± 0.42 μM. The neuroprotective effects of compounds 1 and 3 against H₂O₂-induced apoptosis were assessed in human neuroblastoma SH-SY5Y cells. Compound 1 demonstrated cytotoxicity with IC₅₀ values of 72.37 ± 0.20 μM against K562 and 61.47 ± 0.22 μM against BEL-7402, while compounds 2 and 3 showed cytotoxicity across all five tested human cancer cell lines. Full article

Urban stormwater management systems are increasingly strained by rapid urbanization and climate change, yet existing planning approaches often lack holistic optimization frameworks that account for both green and grey infrastructure (GREI) under uncertain future conditions. This study introduces a multi–objective optimization framework for Grey–Green Infrastructure (GGI), which integrates green infrastructure (GI) with GREI to enhance urban flood resilience, cost efficiency, and adaptability. The framework addresses life cycle cost (LCC), technological resilience (Tech-R), and operational resilience (Oper-R), offering a comprehensive approach to navigating the complexities of urban stormwater management. Key findings reveal that: (1) GGI systems optimized for resilience achieve a 33% improvement in Oper-R, with only a marginal increase in LCC of less than 9%, highlighting their robustness under GREI failure scenarios; (2) the integration of bioretention cells (BCs) and porous pavements (PPs) into GGI increases Tech-R by 7.1%, enhancing soil water retention and permeability, particularly in densely urbanized contexts; and (3) decentralized GGI systems exhibit superior adaptability to extreme weather events, with Design D reducing LCC to USD 53.9 M while maintaining no overflow under a 5–year rainfall event. The framework was validated in Zhujiang New Town, Guangzhou, where optimized GGI designs reduced average pipe diameters and manhole depths by 0.2–0.3 m compared to GREI–only systems, demonstrating both cost and resilience advantages. These findings provide decision–makers with a robust tool for evaluating trade–offs in stormwater infrastructure planning, advancing sustainable urban water management. Full article

Background: Since 2011, re-emerging pseudorabies virus (PRV) variant strains have been widespread in swine herds immunized with the classical PRV vaccine in China, suggesting that it is necessary to develop a new vaccine against these PRV variant strains. Methods: Here, based on a PRV mutant strain isolated in Jinmen (JM), two recombinant strains were constructed using CRISPR/Cas9 technology, including PRV-JM-ΔEK with the deletion of the gE and TK genes and PRV-JM-ΔEI92K with the deletion of the gE, gI, US2, US9, and TK genes. Results: A one-step growth curve and plaque assay revealed that the cell-to-cell transmission ability of PRV-JM-ΔEI92K was lower than that of PRV-JM-ΔEK. However, the replication ability of PRV-JM-ΔEI92K was approximately 10 times higher than that of PRV-JM-ΔEK, similar to wild-type PRV-JM. The intramuscular injection of 10⁶ TCID₅₀ of PRV-JM-ΔEK or PRV-JM-ΔEI92K could not cause death in mice, and both could produce specific antibodies against gB and gD. The survival rate of mice immunized with both recombinant viruses was 100% when the mice were challenged by the PRV-JM strain. Histopathological sections from the PRV-JM-ΔEK group showed milder pathological changes compared to the PRV-JM-ΔEI92K group, proving that PRV-JM-ΔEK provided more effective protection. In pigs injected with 10⁶ TCID₅₀ of PRV-JM-ΔEK or PRV-JM-ΔEI92K, their body temperature did not rise, and their weight gain was not affected. Both recombinant viruses could induce the production of gB- and gD-specific antibodies and neutralizing antibodies. After the challenge of the PRV-JM virus, neutralizing antibody production was rapidly induced and lasted for at least 3 weeks. Pigs immunized with both PRV-JM-ΔEI92K and PRV-JM-ΔEK had a 100% survival rate, demonstrating that both recombinant viruses could provide effective protection. Conclusions: Compared with PRV-JM-ΔEK, PRV-JM-ΔEI92K had better safety. In conclusion, we constructed two PRV recombinant viruses, which have the potential to be used as a live carrier vaccine. Full article

Four new kaurane-type diterpenoids, geliboluols A–D (1–4), along with one known analog (5), were isolated from the culture broth of the marine-derived rare actinomycete Actinomadura geliboluensis. The structures of compounds 1–4 were determined by spectroscopic analysis (HR-ESIMS, 1D, and 2D NMR), the MPA method, and by comparing their optical rotation values with those in the literature. The new compounds were evaluated for their cytotoxicity against seven blood cancer cell lines by a CellTiter-Glo (CTG) assay and six solid cancer cell lines by a sulforhodamine B (SRB) assay. Among the new compounds, compound 4 exhibited moderate cytotoxic activity against some blood cancer cell lines, with GI₅₀ values ranging from 2.59 to 19.64 µM, and against solid cancer cell lines with GI₅₀ values ranging from 4.34 to 7.23 µM. Full article

The salinity of water and soil is a constraint that has an extreme effect on germination and the establishment of crops. Therefore, it is pivotal to boost crop salt tolerance in global semi-arid regions. By mixing Si in an ME medium, a new complex of nanoparticles (Si-CTS-HPC-ME NPs) was synthesized, and we investigated the role of Si-CTS-HPC-ME NPs on Cyamopsis tetragonoloba germination and tolerance against salinity stress. Thus, this study examined the influence of Si-CTS-HPC-ME NPs at different concentrations (N₁: 0, N₂: 40 and N₃: 80 mg L⁻¹) on some germination and seedling growth parameters and the ion homeostasis of Cyamopsis tetragonoloba L. (cluster bean) seedlings under three salinity levels (S₁: 0, S₂: 6 and S₃: 12 dS m⁻¹). With increasing salinity, the energy of germination (GE), index of germination (GI), index of vitality (VI), seedling vigor index (SVI), fresh weight (SFW) and dry (SDW) weight of seedlings, plumule length (PL), and radicle length (RL) parameters gradually decreased, while the mean germination time (MGT) and coefficient of velocity of germination (CVG) increased in salt-stressed cluster bean seedlings in comparison to the control. However, the usage of Si-CTS-HPC-ME NPs was effective in enhancing cluster bean tolerance to salinity by enhancing total phenols and flavonoids and improving K⁺, Si, and Ca²⁺ uptake, thus reducing lipid peroxidation, decreasing sodium ion uptake and potassium leakage, and promoting germination parameters compared with non-NP-treated seedlings. Meanwhile, 40 mg L⁻¹ Si-CTS-HPC-ME NPs exhibited an effective response in saline conditions compared with the other NP treatments. Consequently, the application of Si-CTS-HPC-ME NPs in salt-stressed cluster bean seedlings can serve as an effective technique to enhance salinity tolerance in saline conditions under arid and semi-arid climatic conditions. Full article