Search Results (36)

Light availability in tropical forests varies spatially and temporally, strongly influencing plant acclimation. Understanding variation and covariation among functional traits associated with photoacclimation is essential for predicting plant responses to environmental change. Here, we investigated acclimatory responses of Schinus terebinthifolia Raddi (Anacardiaceae), a widespread Neotropical species adapted to heterogeneous light environments. We evaluated variation and covariation in morphological, anatomical, physiological, and nutritional traits under contrasting light conditions. Under high light, plants invested more resources in palisade parenchyma and subepidermal layers while maintaining water-use efficiency, indicated by higher δ¹³C values. Irregular adaxial cuticles and unstacked thylakoid membranes were also observed under high irradiance. The strongest covariation occurred among anatomical traits, especially spongy parenchyma and adaxial and abaxial cuticles. Overall, the relationship between trait variation and covariation was slightly negative but not significant, although patterns differed among functional groups. These findings demonstrate that photoacclimation in S. terebinthifolia involves coordinated functional strategies that optimize light modulation, water conservation, and photosystem II performance under variable tropical light environments. Full article

In transitional tropical ecosystems such as the Amazonia–Cerrado ecotone, dominant tree species experience strong environmental heterogeneity, requiring coordinated functional strategies to cope with drought, nutrient limitation, and disturbance. However, how these species integrate leaf morphoanatomical traits and wood density to persist in such environments remains poorly understood. We assessed the coordination among leaf anatomical and morphological traits and their relationship with wood density in five dominant tree species across three savanna park sites in the Amazonia–Cerrado transition. Morphological traits included leaf thickness, specific leaf area, leaf dry matter content, and wood density, alongside 17 anatomical leaf traits. We analyzed inter- and intraspecific variation and covariation patterns to identify trait-based ecological strategies along the acquisitive–conservative spectrum. We found strong coordination among traits related to protection (e.g., cuticle thickness and trichomes) and resource use, as well as clear alignment between leaf and wood traits. Species identity explained most trait variation, although leaf thickness showed notable intraspecific plasticity. Species with conservative traits exhibited thicker leaves and higher wood density, whereas species with acquisitive strategy showed higher specific leaf area and lower leaf dry matter content. Overall, trait coordination reflects integrated ecological strategies shaped by environmental heterogeneity, highlighting the role of multi-trait syndromes in driving functional adaptation in ecotonal systems. Full article

Modern changes in natural and anthropogenic conditions in mountain ecosystems highlight the growing need to assess the status of medicinal plant populations and factors influencing their resilience. This study presents findings from a comprehensive investigation of three previously undocumented wild populations of Chelidonium majus L., discovered in the gorges of Kaindy, Shet-Merke, and Kolsai in the eastern part of the Kungey Alatau range. The research included analysis of phytocenotic conditions, population age structure, morphometric and morpho-anatomical traits of plants, along with physicochemical properties of soils. It was found that C. majus is associated with meadow-type moisture regimes and occurs spottily, mainly in moist microhabitats within open and semi-shaded plant communities. Population sizes ranged from 264 to 296 individuals, with average densities between 5.1 and 16.3 individuals per m². All studied populations exhibited complete ontogenetic spectra, dominated by generative stages (56.1–67.2%), indicating preserved reproductive potential despite limited recruitment at early developmental phases. Morpho-anatomical analysis revealed high phenotypic plasticity: under drier, poorer soil conditions, xeromorphic features developed, whereas mesomorphic structures prevailed in more favorable habitats. Soil analysis indicated that Shet-Merke population enjoys optimal growth and regeneration conditions. These results suggest that current C. majus populations in the region remain relatively stable, though strongly dependent on edaphic–hydrological and phytocenotic factors—underscoring the necessity of integrated monitoring to ensure conservation of medicinal plant resources. Full article

Background/Objectives: Along the Tyrrhenian coast of central Italy, multilayered caves have yielded significant Neanderthal-era human remains. Recent excavations at Guattari Cave uncovered hominin fossils dated to approximately 66–65 ka, revealing a population with notable morpho-anatomical variability exhibiting both plesiomorphic (primitive) and autapomorphic (derived) traits. Methods: Here we present detailed morphometric and comparative analyses of cranial, dental, and postcranial remains, demonstrating affinities with Homo erectus (sensu stricto [s.s.] and lato [s.l.]), Proto-Neanderthals, classical Neanderthals, and Homo sapiens. Results: These findings indicate notable morpho-anatomical variability among the Guattari Cave hominin remains, with affinities to multiple hominin lineages during the Middle and Late Pleistocene. Pleistocene. Conclusions: The Guattari Cave assemblage thus contributes to our understanding of Eurasian hominin diversity and evolutionary dynamics, highlighting the Mediterranean as a region of interest for studying the phyletic continuity and diversity preceding modern humans. Full article

In Romania’s wild flora, several Iris species exhibit important ornamental characteristics, such as early spring flowering and resilience to abiotic stress. This study assessed the behavior to new ecological conditions, the ornamental potential, and the antioxidant capacity of the wild species of Iris brandzae using morpho-anatomical, physiological, and biochemical biomarkers. The study of phenotypic characteristics (number and size of leaves on sterile and fertile shoots, size of flowering stems, bracts protecting the flowers, and perianth-segments) aimed to confirm and supplement existing information in the literature, as well as to evaluate the ornamental potential of this species. Morphological analyses revealed clear differences between fertile and sterile shoots, while photosynthetic activity across phenophases showed values within normal parameters, with the maximum recorded during flowering and with the chlorophyll a/chlorophyll b ratio maintained at values close to 3:1, indicating favorable cultivation conditions. Biochemical investigations (total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity) demonstrated that dried plant material, particularly roots, contained higher levels of phenolic and flavonoid compounds and exhibited stronger antioxidant activity compared to fresh material. By integrating morpho-anatomical, physiological, and biochemical data, this research provides the first comprehensive characterization of I. brandzae beyond taxonomic and ecological descriptions. Our findings emphasize the species behavior under cultivation conditions, its ornamental value, and its potential as a source of bioactive compounds relevant to pharmaceutical applications. Full article

We evaluated the effects of suspensions of magnetite–maghemite nanocomposites functionalized with quaternized chitosan and phosphate groups on morpho-anatomical and physiological traits of yellow maize (Zea mays) and chili pepper (Capsicum annuum) seeds. A phytotoxicity assay was first conducted by applying aqueous suspensions of these nanocomposites to maize seeds at iron concentrations up to 100 ppm, using deionized water as the control under humid chamber conditions. After incubation, seeds treated with concentrations above 100 ppm exhibited reductions in biomass and root length compared with the control, suggesting phytotoxicity at high levels. Based on these results, suspensions containing 25, 35, and 45 ppm of iron, with corresponding phosphorus concentrations of 2.9, 4.0, and 5.2 ppm, were selected for a second in vitro assay using both maize and chili seeds. No statistically significant differences between treatments and control were detected for the variables measured. Germinated seeds from this assay were further evaluated under greenhouse conditions, where measured parameters also showed no significant differences between treatments and control in either crop. Overall, the findings indicate that aqueous suspensions of magnetite–maghemite nanocomposites with iron concentrations below 100 ppm do not produce phytotoxic effects on seed germination or morpho-anatomical and physiological traits measured. Full article

Global climate change has intensified the frequency, intensity, and spatial heterogeneity of drought events, posing severe threats to the stability of terrestrial ecosystems. Plant drought resilience, which encompasses a plant’s capacity for drought resistance, post-stress recovery, and long-term adaptation and transformation to sustain ecosystem functionality, has emerged as a central focus in botanical and ecological research. This review synthesizes the conceptual evolution of plant drought resilience, from early emphasis on resistance and recovery to the current multi-dimensional framework integrating adaptation and transformation, and synthesizes advances in understanding multi-scale drought resilience in terrestrial plants—spanning molecular, physiological, individual, community, and ecosystem levels. Key mechanisms include molecular/physiological adaptations (osmotic adjustment, antioxidant defense, hydraulic regulation, carbon–water reallocation via gene networks and aquaporins), morpho-anatomical traits (root architectural plasticity, leaf structural modifications, and hydraulic vulnerability segmentation), community/ecosystem drivers (biodiversity effects, microbial symbioses, and soil–plant–feedback dynamics). We critically evaluate quantitative metrics and expose critical gaps, including neglect of stress legacy effects, oversimplified spatiotemporal heterogeneity, and limited integration of concurrent stressors. Future research should prioritize multi-scale and multi-dimensional integrated analysis, long-term multi-scenario simulations with field validation, and harnessing plant–microbe interactions to enhance drought resilience, providing a theoretical basis for ecosystem sustainability and agricultural production under climate change. Full article

(1) Background: High temperatures and water scarcity, intensified by climate change, present major challenges to Mediterranean viticulture. In this study morphological, anatomical, and physiological responses of leaves at two developmental stages of Vitis vinifera L. cv. Assyrtiko were examined. (2) Methods: Young expanding and fully expanded leaves of two-year-old grapevines grown in pots in a controlled growth chamber, grown in soil in an open-air vineyard, and an adjacent greenhouse on Aegina Island (Greece), were used. The plants were subjected to water deficit (WD), elevated temperature (ET), and combined water deficit and elevated temperature (WD_ET) conditions for four months, and compared with control plants (C). (3) Results: Stress conditions caused contrasting effects on specific leaf area (SLA), which declined in young expanding leaves, except for a significant increase under elevated temperatures, while it increased in fully expanded leaves exposed to stress treatments. Concerning the anatomical traits, the thickness of spongy and palisade parenchyma did not change in young expanding leaves exposed to the three stress treatments, but increased in fully expanded leaves exposed to WD_ET. Metabolic traits (phenolic compounds, soluble sugars, and starch content) further highlighted these differences. (4) Conclusions: The findings reveal distinct stress responses between young expanding and fully expanded leaves of the grapevine Assyrtiko, underscoring the influence of water deficit and elevated temperatures on leaf developmental stage. Full article

Recent sampling efforts in northern Algeria and the investigation of some collection material has revealed the presence of taxa with conchological and genital morphological similarities to the “Candidula-like” group. A detailed investigation of the anatomy of the genitalia combined with the analysis of two mitochondrial and two nuclear markers of representatives of the known genera as well as two Algerian taxa has been conducted. The result confirms the affiliation of the latter in the “Candidula-like” complex. Comparative anatomical analysis led to the description of a new genus, Kherattolactea gen. nov., endemic to northern Algeria with Xerophila keratae Kobelt 1892 as the type species. The anatomical distinction corroborates the result of the molecular analysis that reveals apomorphic nucleotide substitution. Moreover, the result of the combined analysis provides the first confirmed record of the genus Orexana Chueca, Gómez-Moliner, Madeira & Pfenninger, 2018, in north Africa, represented by the species Orexana lemoinei (Kobelt, 1882) nov. comb. A new Xerogyra species, Xerogyra hallgassi nov. sp., from the Apennine Mountain has been described based on the combination of morphoanatomical and molecular traits. For the first time, the morphoanatomical description of the known Xerogyra species has been presented. Full article

The necessity of optimizing the nutrient and water efficiency in conventional hydroponics and enhancing their sustainability has given rise to the concept of cascade cropping systems. These achieve high water and resource use efficiencies, together with a lower environmental footprint, which is especially important for Mediterranean areas. However, scientific questions about the mechanisms that drive productivity in this system remain to be answered. This study aimed at a comprehensive evaluation of crop performance in cascade systems in terms of morphoanatomical and functional responses, also including product quality parameters, which influence the marketability of the fruit. In a three-month experiment, the dynamics of melon’s photosynthetic light use efficiency, pigment contents, growth parameters, and leaf compactness were assessed in a cascade system using drainage of tomato cultivation in comparison to classic hydroponic melon. The fruits’ chroma, hardness, total soluble solids, and pH were also measured. Comparable plant functional responses in the control and cascade melon plants resulted in similar growth and morphoanatomical traits. The fruit quality attributes were also found to be almost identical. It is proposed that the cascade system is both effective and sustainable in regions facing climatic and water scarcity pressures, such as those that are prevalent around the Mediterranean basin. Full article

Picea omorika (Pančić) Purk., (Serbian spruce) is a relic, endemic, and vulnerable conifer that remains insufficiently studied to date. To the best of our knowledge, this is the first report on the morpho-anatomical and phytochemical diversity of needles from three populations in Bosnia and Herzegovina. The length of two-year-old needles was measured with a digital caliper. The next six properties were measured based on cross-sections of the needles using a light microscope. An analysis of volatile compounds was carried out using gas chromatography coupled with mass spectrometry (GC-MS) and flame ionization detection (GC-FID). The highest values of needle traits were found in the Viogor population, with the lowest in the Tisovljak population, which was statistically confirmed. There was also a significant difference between needles from Bosnia and Herzegovina and those from Serbia. Bornyl acetate, camphene, limonene, and α-pinene were identified as the major terpene compounds. Multivariate analyses also suggested a tendency toward the separation of the Tisovljak population. A statistical comparison of three Bosnian and Herzegovinian and four Serbian populations (previously studied and published) revealed two distinct groups: (1) three Bosnian populations and the Vranjak population from Serbia, and (2) three populations from Serbia—Štula, Zmajevački Potok, and Mileševka Canyon. The general conclusions are that divergence in needle morpho-anatomy aligns with divergence in needle chemistry and that Bosnian and Herzegovinian populations are distinct from nearly all Serbian populations. Full article

The effect of soil water restriction on the root structure and morpho-anatomical attributes of Lolium perenne L. (Lp) and Bromus valdivianus Phil. (Bv) was investigated. The anatomical structure of roots from plants grown under two water restriction conditions (20–25% and 80–85% field capacity (FC)) were assessed using paraffin embedding and thin sections. These sections were examined to assess anatomical traits, including root diameter (root D), stele diameter (stele D) and cortex thickness (cortex T), and xylem vessel of Lp and Bv roots. Tiller population, shoot herbage mass, and the shoot-to-root ratio were also determined. Under water restriction, biomass and tillers were significantly decreased (p < 0.001), while the root-to-shoot ratio significantly increased, indicating a higher proportion of Bv roots than shoots when compared to Lp. The root D and stele D, and cortex T, were larger in Bv than in Lp (p < 0.001), indicating a greater adaptation of Bv for water uptake and storage compared to Lp. Xylem vessels were wider in Lp when compared to Bv (p < 0.01), indicating greater water flow within the plant. Water restriction generated a decrease in root D, stele D, and cortex T (p < 0.01). Canonical variate analysis showed that the pith cell wall had a strong positive relationship with water restriction in both Bv and Lp; lignified xylem and the endodermis wall had a close relationship with Lp under water restriction. The findings demonstrate that Lp and Bv have individual structural and morpho-anatomical response strategies to increasing water restriction. Full article

Chenopodium hircinum, the putative wild ancestor of quinoa, is a source of traits that could improve the tolerance of crop quinoa to high temperatures. However, seeds of C. hircinum have physiological dormancy (PD), which is an obstacle for plant propagation and use in breeding programs. We studied the intraspecific variability in morpho-anatomical traits of embryo covering structures and their association with PD. We also evaluated the effects of different dormancy-breaking treatments on PD alleviation and germination. Seeds were dispersed with a remnant perianth and a persistent pericarp that could be removed by scraping. The seed coat was formed by palisade cells impregnated with tannins, and the seed contained a thin layer of peripheral endosperm surrounding the embryo. In our investigation, the thickness of the pericarp (P) and/or seed coat (SC) varied among populations. Populations with higher P and/or SC thickness showed lower percentages of germination and water absorption. The combined dormancy-breaking treatment (bleach + perforated coverings + gibberellic acid) promoted dormancy release and increased germination. C. hircinum seeds showed non-deep physiological dormancy. Based on previous knowledge about quinoa, and our results, we conclude that embryo coverings, especially the seed coat, have an important role in dormancy control, imposing a mechanical restraint on radicle emergence. Full article

Studies on the genetic diversity of coffee trees are important, considering their role in the maximization of productivity and quality. However, the success of a breeding program depends on the existence of genetic variability in the population under study. Our study aimed to evaluate the genetic diversity of the morpho-agronomic and anatomical leaf traits of different cultivars of Coffea arabica grown in the Gorongosa mountain region, in the area of Gorongosa National Park, Mozambique. The experiment assessed nine coffee cultivars based on their morpho-agronomic and anatomical traits. The plagiotropic branch diameter, leaf dry mass, leaf mass per area, number of epidermal cells + stomata, and stomatal form indicated differences among the studied cultivars. Among the nine C. arabica cultivars grown in the mountainous region of Gorongosa National Park, Mozambique, low genetic variability in morpho-agronomic traits was detected, while the genetic variability in leaf anatomical traits was higher. The nine cultivars were segregated into two groups, one consisting of Catucaí Amarelo 2SL and Catuaí Vermelho IAC 81, and the second consisting of Catuaí Amarelo IAC 39, Catucaí Vermelho 19/8, Acauã, Catucaí 785-15, Costa Rica, Catimor 128, and Catuaí Vermelho IAC 44. The cultivar segregation into the two groups indicated that the morpho-anatomical traits can be considered during the selection stages in breeding programs. Full article

Cold acclimation before winter has been shown to enhance the cold tolerance of evergreen conifers, including Pinus massoniana Lamb., a characteristic heteroblastic foliage tree in the conifer. In the initial growing season of P. massoniana, both primary needle seedlings (PNSs) and secondary needle seedlings (SNSs) are generated. While previous research has highlighted differences in the morphological structure and photosynthetic physiological functions of primary and secondary needles, their response to cold acclimation remains poorly understood. This study aimed to investigate the changes in morpho-anatomical structure, starch grain accumulation, and lignin deposition in the roots, stems, and leaves of PNSs and SNSs during cold acclimation using solid potassium iodide and hydrochloric acid phloroglucinol double-staining techniques. The results revealed that, during cold acclimation, the leaves and stems of PNSs exhibited sensitivity to low-temperature stress, resulting in noticeable shrinkage and fracture of mesophyll and cortical parenchyma cells. Furthermore, the early stages of cold acclimation promoted the accumulation of starch grains and lignin in the seedling tissues. In contrast to PNSs, the leaves and stems of SNSs exhibited a shorter cold acclimation period, attributed to the hydrolysis of starch grains in the epidermal cell walls and the transformation of xylem lignin, which supports cell structure stability and enhances cold resistance. In conclusion, these findings suggest that SNSs displayed a superior cold resistance potential compared to PNSs following cold acclimation, providing a significant theoretical basis for the further screening of cold-tolerant germplasm resources of P. massoniana and the analysis of cold resistance traits in heteroblastic foliage. Full article