Search Results (423)

Organoids allow to model healthy and diseased human tissues. and have applications in developmental biology, drug discovery, and cell therapy. Traditionally cultured in immersion/suspension, organoids face issues like lack of standardization, fusion, hypoxia-induced necrosis, continuous agitation, and high media volume requirements. To address these issues, we developed an air–liquid interface (ALi) technology for culturing organoids, termed AirLiwell. It uses non-adhesive microwells for generating and maintaining individualized organoids on an air–liquid interface. This method ensures high standardization, prevents organoid fusion, eliminates the need for agitation, simplifies media changes, reduces media volume, and is compatible with Good Manufacturing Practices. We compared the ALi method to standard immersion culture for midbrain organoids, detailing the process from human pluripotent stem cell (hPSC) culture to organoid maturation and analysis. Air–liquid interface organoids (3D-ALi) showed optimized size and shape standardization. RNA sequencing and immunostaining confirmed neural/dopaminergic specification. Single-cell RNA sequencing revealed that immersion organoids (3D-i) contained 16% fibroblast-like, 23% myeloid-like, and 61% neural cells (49% neurons), whereas 3D-ALi organoids comprised 99% neural cells (86% neurons). Functionally, 3D-ALi organoids showed a striking electrophysiological synchronization, unlike the heterogeneous activity of 3D-i organoids. This standardized organoid platform improves reproducibility and scalability, demonstrated here with midbrain organoids. The use of midbrain organoids is particularly relevant for neuroscience and neurodegenerative diseases, such as Parkinson’s disease, due to their high incidence, opening new perspectives in disease modeling and cell therapy. In addition to hPSC-derived organoids, the method’s versatility extends to cancer organoids and 3D cultures from primary human cells. Full article

Background: The purpose of this study was to evaluate Achilles tendon ruptures (ATR) in NHL players and the effects on return to play and player performance metrics. The incidence, mechanism of injury, management strategy, return to play (RTP), and post-injury were assessed from official online sports databases. Methods: A retrospective review of NHL players who sustained a partial or complete tear of the Achilles tendon from 2008 to 2024 was performed. Data were collected from NHL injury databases and media reports, and included player demographics, injury mechanism, treatment, and post-injury performance metrics. A Wilcoxon signed rank test was used to compare pre-injury and post-injury performance metrics, with significance set at p < 0.05. Results: Here, 15 NHL players with a mean age of 27.8 years were identified, with a prevalence rate of 0.125 injuries per 10,000 athletic exposures. Overall, 73.3% of ATRs were non-contact in nature, with 60.0% of ATRs occurring during off-season training. Fourteen players were managed with non-operative treatment, with no re-ruptures reported. The RTP rate was 93.3%, with players missing a mean number of 45.7 games. However, there was a deterioration in post-injury performance metrics, including games played per season, plus/minus rating, and time on ice per game post-injury. Conclusions: This study found that Achilles tendon ruptures are an uncommon injury in NHL players, with a prevalence rate of 0.125 injuries per 10,000 athletic exposures. A high RTP rate of 93.3% was observed in this cohort. However, there was a deterioration in post-injury performance metrics, including games played per season, plus/minus rating, and time on ice per game post-injury, highlighting the potential devastating sequelae of ATRs in elite NHL athletes. Full article

To meet the requirements for radar echo acquisition and feature extraction from stratified media and rough-surfaced targets, a vehicle was geometrically modelled in CAD. Monte Carlo techniques were applied to generate the rough interfaces at air–snow and snow–soil boundaries and over the vehicle surface. Soil complex permittivity was characterized with a four-component mixture model, while snow permittivity was described using a mixed-media dielectric model. The composite electromagnetic scattering from a rough-surfaced vehicle on snow-covered soil was then analyzed with the finite-difference time-domain (FDTD) method. Parametric studies examined how incident angle and frequency, vehicle orientation, vehicle surface root mean square (RMS) height, snow liquid water content and depth, and soil moisture influence the composite scattering coefficient. Results indicate that the coefficient oscillates with scattering angle, producing specular reflection lobes; it increases monotonically with larger incident angles, higher frequencies, greater vehicle RMS roughness, and higher snow liquid water content. By contrast, its dependence on snow thickness, vehicle orientation, and soil moisture is complex and shows no clear trend. Full article

Black crusts and biological colonisation are among the most common types of ‘diseases’, with diverse aetiologies and presentations, affecting masonry architectural heritage. Over the past decades, there has been an increase in the incidence of this degradation phenomena due to the increase in pollution and climate change, especially on the urban walls of ancient cities. In particular, the present research examines the state of conservation of the city walls of Perugia, which are divided into two main city walls dating back to the Etruscan and Medieval periods and are recognised as historical heritage of high identity and cultural value. The degradation reflects, in the mentioned cases, on the liminal public and green areas. A view is also reflected in local journalism and social media, where residents and visitors have framed the spontaneous growth of herbs and medicinal shrubs within the stone joints of historic walls as an apparently benign and aesthetically pleasing occurrence. This misleading interpretation, while rooted in a superficial aesthetic appreciation, nevertheless draws attention to a real and urgent issue: the pressing need for systematic maintenance and intervention strategies—coordinated between academics, students, designers and stakeholders—which are able to reposition the city walls as central agents of urban and cultural regeneration, rather than peripheral remnants of the past. Full article

Background and Objectives: Visceral arterial pseudoaneurysms (VAPAs) are rare vascular lesions characterized by the disruption of partial disruption of the arterial wall, most commonly involving the intima and media. They have an estimated incidence of 0.1–0.2%, with the splenic artery most commonly affected. Their management poses unique challenges due to the high risk of rupture. Timely recognition is crucial, as unmanaged pseudoaneurysms have a mortality rate of 90%. This narrative review aims to synthesize current knowledge regarding the epidemiology, etiology, clinical presentation, diagnostic methods, and management strategies for VAPAs. Materials and Methods: A literature search was performed across Pubmed for articles reporting on VAPAs, including case reports, review articles, and cohort studies, with inclusion of manuscripts that were up to (date). VAPAs are grouped by embryological origin—foregut, midgut, and hindgut. Results: Chronic pancreatitis is a primary cause of VAPAs, with the splenic artery being involved in 60–65% of cases. Other causes include acute pancreatitis, as well as iatrogenic trauma from surgeries, trauma, infections, drug use, and vascular diseases. VAPAs often present as abdominal pain upon rupture, with symptoms like nausea, vomiting, and gastrointestinal hemorrhage. Unruptured pseudoaneurysms may manifest as pulsatile masses or bruits but are frequently asymptomatic and discovered incidentally. Diagnosis relies on both non-invasive imaging techniques, such as CT angiography and Doppler ultrasound, and invasive methods like digital subtraction angiography, which remains the gold standard for detailed evaluation and treatment. A range of management options exists that are tailored to individual cases based on the aneurysm’s characteristics and patient-specific factors. This encompasses both surgical and endovascular approaches, with a growing preference for minimally invasive techniques due to lower associated morbidity. Conclusions: VAPAs are a critical condition requiring prompt early recognition and intervention. This review highlights the need for ongoing research to improve diagnostic accuracy and refine treatment protocols, enhancing patient outcomes in this challenging domain of vascular surgery. Full article

Ischemic stroke following percutaneous coronary intervention (PCI) is a rare complication, with an overall incidence of 0.56%. Most embolic strokes result from the dislodgement of atherosclerotic plaques, thrombi formed on catheter surfaces, procedural maneuvers, or, less commonly, air or metallic emboli originating from fractured guidewires. We present a unique case of stroke following PCI due to a previously unreported mechanism—arterial tunica media embolization associated with arterial access. A 57-year-old female presented with chest pain at rest and with exertion, accompanied by episodes of anxiety and fluctuating blood pressure, for which coronary angiography was performed, revealing 90–99% stenosis of the left anterior descending artery and necessitating PCI. During the procedure, the patient developed an eye deviation, aphasia, and left-sided hemiparesis. Cerebral angiography identified a M2 segment occlusion of the right middle cerebral artery (MCA) and a subocclusion of the right anterior cerebral artery (ACA). Thrombectomy was performed, retrieving two white, tubular emboli resembling fragments of a vessel wall, histologically confirmed to be arterial tunica media. While PCI is associated with a low complication rate, its increasing frequency necessitates awareness of emerging complications. This case underscores a previously undocumented potential embolic complication arising from the performance of PCI. Full article

Background/Objectives: The economic impact of adverse events following COVID-19 immunisation (AEFIs) in Australia is underexplored. This study aimed to assess the economic burden of AEFIs on both healthcare systems and societal productivity. Methods: A cross-sectional survey was conducted in Queensland residents aged ≥18 years who had received at least one dose of a COVID-19 vaccine in the preceding 12 months. Overall, 6964 participants were recruited from July to September 2022 via email and broad social media campaigns. The survey collected data on the incidence, type and duration of AEFIs; healthcare utilisation; and work-related absenteeism. Healthcare costs were estimated using national healthcare reimbursement data, and productivity costs were estimated using Australian Bureau of Statistics Average Weekly Earnings. Results: Of the 6797 eligible respondents (predominantly female [62%]; median age: 52 years), AEFIs were reported by 53.4%, 44.1%, 40.7%, and 40.9% following doses 1 to 4, respectively. Pain and tenderness were predominant local AEFIs, while tiredness and headaches were the most frequent systemic AEFIs, generally resolving within three days. Relatively few participants reporting AEFIs consulted medical professionals: 7.0%, 7.3%, 5.1%, and 1.9% following each dose, respectively. The mean healthcare cost per person reporting AEFIs was AUD 24, AUD 88, AUD 22, and AUD 4 following each respective dose. Work absenteeism was recorded in 16.5%, 18.2%, 15.2%, and 11.2% following each dose with mean absenteeism days per person of 4.7, 7.4, 3.6 and 2.1, respectively, and mean productivity costs per person reporting AEFIs amounting to AUD 1494, AUD 2388, AUD 1136, and AUD 690, respectively. Conclusions: Participants reported mostly mild AEFIs with only a small proportion of individuals seeking medical services. Productivity costs attributable to these AEFIs exceeded direct healthcare expenses incurred. Full article

This paper investigates crash severity in single-vehicle road crashes involving collisions with roadside light poles in Croatia. Due to the absence of detailed object-type classifications in the official crash database, media reports were used to identify relevant incidents in combination with the official state database, resulting in 38 crashes identified between 2016 and March 2025. Descriptive analysis and crosstabulation were applied to explore patterns in crash outcomes. A CHAID decision tree analysis was then applied in an exploratory capacity to highlight possible predictors of injury or fatal outcomes, acknowledging the limitations of the small sample size. Results showed that the speed limit was the only variable significantly associated with crash severity, with all crashes above 50 km/h resulting in injuries or fatalities. The findings highlight the importance of speed management and support the potential for implementing passively safe poles to reduce the consequences of such crashes. The study also discusses the performance of different pole types in line with EN 12767:2019, defines risk zones, and proposes solutions for the example locations. The results offer future research implications and valuable insights for road safety improvement, especially in areas with frequent pole collisions. Full article

Violence against people with albinism (PWAs) in Tanzania continues nearly two decades after mass media reported the first incidents in the mid-2000s. The violence is linked to organ trafficking for use in “magical rituals” that allegedly help politicians and businesspeople to succeed in their endeavors. Over time, as societal awareness grows, the attacks become increasingly clandestine and complex. PWAs themselves, the public, and gray literature frequently relate the violence to the increased political and economic activity and participation following Tanzania’s adoption of political and economic liberalization. However, scholarly research is either silent or mentions the occult practices only in passing. This paper, therefore, explores Tanzania’s institutional arrangements both driving the violence and crippling the efforts at promoting the rights and welfare of PWAs in the wake of increasing political and economic participation in the country. It discusses the ways in which violence against PWAs has evolved alongside political and economic dynamics from the time such incidents came to public attention until the present. I argue that the current approach, whereby advocacy about the rights of PWAs relies on appeasing the state, appears to perpetuate the very beliefs and practices driving the violence. The exploration makes use of first-hand experience through my participation in numerous formal and informal interactions with PWAs, internal and external meetings within the Tanzania Albinism Society (TAS), interviews, and gray literature on the subject. Full article

Exploring the neurogenic potential of extraneural stem cells under the actions of proneurogenic biomolecules may enhance the success of autologous cell therapy for neurodegenerative diseases such as Parkinson’s. Neural stem and progenitor cells (NSPCs) from extraneural tissues have emerged as potential sources of functional dopaminergic (DA) neurons. Background/Objectives: This study aimed to generate DA neurons from ovarian cortical cells (OCC)-derived NSPCs to elucidate whether follicle-stimulating hormone (FSH) can enhance this process and to evaluate the electrophysiological functionality of differentiated neural cells using the patch-clamp technique. Methods: OCC-NSPCs were differentiated towards the DA pathway during the neurosphere (NS) assay after two culture periods for cell expansion (CEP-1, CEP-2) with one of these media: M1 (positive control with epidermal growth factor, EGF, and fibroblast growth factor2, FGF2), M2 (control), and M3 (M2 with FSH, 50 ng/mL). Image analysis, morphometric evaluation, cell proliferation assays, and gene expression analysis of NSPC-specific transcripts were performed. After CEP-2, NS cells were cultured for 30 days in a serum-free medium containing Sonic-Hedgehog, FGF2, FGF8, and brain-derived neurotrophic factor (BDNF) for differentiation. At the end of culture, expression, and immunolocalization of GFAP, Olig2, NeuN, and tyrosine hydroxylase (TH) were analyzed in cells, along with patch-clamp recordings in differentiated neurons. Results: Cell proliferation and NS development were larger in OCC-NSPCs from groups M1 and M3 than in M2. Expression of NSPC-related transcripts was higher in M2; however, M1 and M3 cultures showed greater expression of differentiation markers NeuN, GFAP, Olig2, and TH. NeuN, GFAP, and TH were immunolocalized in differentiated cells and NS that were generated during differentiation. TH was localized in neural precursor cells, some neurons, core cells of small-, medium-, and large-sized NS, and in cells close to the outer cell layer of large NS, with greatest immunolocalization percentages in NS primed with FSH during CEP-1/2 (M3). Electrophysiological recordings revealed a major incidence of plateau potentials and a significant proportion of complete action potentials, reflecting successful functional neuronal differentiation. Conclusions: DA precursors and functional neurons can be successfully obtained after OCC-NSPCs-directed differentiation. FSH priming during the expansion period enhances the neurogenic potential of these cells towards the DA pathway. Future research will explore the eventual therapeutic use of these findings for neurodegenerative diseases. Full article

The female reproductive tract (FRT) serves as the core of human reproduction, and its health is directly related to population quantity and family happiness. The high incidence rate of female reproductive tract diseases globally poses a severe threat to women’s health. Nevertheless, the exploration of its physiological functions and pathological mechanisms still lacks satisfactory research models. Organoids, as an emerging technology, not only circumvent numerous ethical issues existing in in vivo experiments but also precisely replicate the morphological structure and characteristics of the simulated tissues. The purpose of this article is to summarize the basic paradigms of organoid establishment and their applications in female reproductive research. Specifically, this article summarizes the cell sources, extracellular scaffolds, and culture media used in the establishment of organoids. It also describes the applications and future development prospects of female reproductive tract organoids established in current research in physiological and pathological studies. The importance of organoid technology in the female reproductive tract research cannot be ignored. It has opened up new avenues for research in this field and greatly promoted the exploration of female reproductive health and disease mechanisms. Full article

Background: Health and safety are critical pillars of functional higher education systems. In South African universities, recurring incidents have highlighted vulnerabilities, particularly concerning gender-based violence (GBV), student housing conditions, infrastructure safety, and campus crime. Methods: This study employed a document-based qualitative approach, analysing public records, police reports, campus press statements, and national media coverage of incidents reported at South African universities (2015 to 2024). The study is guided by public health and victimology frameworks to examine systemic risk factors and institutional responses. Results: The findings indicate increased reports of GBV, multiple student deaths related to substandard residence conditions, infrastructure-related fatalities, and a lack of consistent institutional safety policies. A pattern of poor infrastructure management, weak inter-institutional communication, and failure to implement recommendations following major incidents emerged across the dataset. Conclusions: South African universities remain exposed to preventable student risks. Targeted interventions, mandatory safety audits, emergency response units, and accountability structures are urgently needed to address systemic failings and protect student welfare. The study supports Sustainable Development Goals 3 and 4 by linking campus safety to student well-being and equitable access to higher education. Full article

Background/Objectives: Gallstone formation (cholelithiasis) is a common and important consequence following bariatric surgery, though regional data from the Northern Border Region are limited. This study aimed to investigate the incidence and risk factors of gallstones in this population, with the goal of optimizing postoperative treatment and reducing morbidity. Methods: We conducted a cross-sectional study using a non-probability convenience sampling technique to recruit 509 participants with varying degrees of obesity. Four hundred and ten study participants underwent bariatric surgery, of whom 73 were excluded for preoperative cholelithiasis and/or cholecystectomy. Data were collected through a self-administered, pre-validated questionnaire distributed via various social media platforms. These data included demographics, type/timing of surgery, pre/postoperative BMI, medical history, use of gallstone prophylaxis, and gallstone outcomes. Logistic regression analysis was used to identify independent predictors of gallstone formation. Results: Postoperative cholelithiasis developed in 60.8% of patients, most commonly within the first postoperative year, with risk peaking between 7 and 12 months after surgery. Rapid and substantial postoperative weight loss, as reflected in a lower current BMI and a transition to normal or overweight status within one year, was significantly associated with an increased incidence of gallstones. Female sex (OR: 2.62, 95% CI: 1.38–4.98, p = 0.003) and non-use of gallstone prevention medication (OR: 4.12, 95% CI: 1.34–12.64, p = 0.013) were independent predictors of gallstone formation. A longer time since surgery (OR: 0.76, 95% CI: 0.63–0.91, p = 0.004) and a lower current BMI (OR: 0.48, 95% CI: 0.28–0.83, p = 0.008) were associated with a reduced risk. Smoking status and comorbidities were not significantly related to the risk of gallstones. Conclusions: Gallstone formation after bariatric surgery in this population is influenced by female sex, rapid postoperative weight loss, and lack of prophylactic medication, while the type of surgical procedure does not significantly affect risk. Focused monitoring and preventive strategies, particularly in high-risk groups, are recommended to reduce gallstone-related complications following bariatric surgery. Full article

(1) Background: Detecting long-lie incidents—where individuals remain immobile after a fall—is essential for timely intervention and preventing severe health consequences. However, most existing systems focus only on fall detection, neglect post-fall monitoring, and raise privacy concerns, especially in real-time, non-invasive applications; (2) Methods: This study proposes a lightweight, privacy-preserving, long-lie detection system utilizing thermal imaging and a soft-voting ensemble classifier. A low-resolution thermal camera captured simulated falls and activities of daily living (ADL) performed by ten healthy participants. Human pose keypoints were extracted using MediaPipe, followed by the computation of five handcrafted postural features. The top three classifiers—automatically selected based on cross-validation performance—formed the soft-voting ensemble. Long-lie conditions were identified through post-fall immobility monitoring over a defined period, using rule-based logic on posture stability and duration; (3) Results: The ensemble model achieved high classification performance with accuracy, precision, recall, and an F1 score of 0.98. Real-time deployment on a Raspberry Pi 5 demonstrated the system is capable of accurately detecting long-lie incidents based on continuous monitoring over 15 min, with minimal posture variation; (4) Conclusion: The proposed system introduces a novel approach to long-lie detection by integrating privacy-aware sensing, interpretable posture-based features, and efficient edge computing. It demonstrates strong potential for deployment in homecare settings. Future work includes validation with older adults and integration of vital sign monitoring for comprehensive assessment. Full article

There has been a well-documented increase in the incidence of colorectal cancer in patients under 50 years of age. Additionally, these patients present with later-stage cancer at diagnosis compared to their over-50 counterparts. However, there is limited consensus on how the impact of this evolving epidemiology should impact existing prevention and screening tools. Recently proposed strategies include increased genetic testing, improved young patient awareness through targeted media campaigns, and initiatives to increase clinical suspicion in primary care providers. Prevention is further complicated by nuances of treating colorectal cancer in the younger population, with underexplored concerns regarding fertility, sexual health, financial impact, and extended post-treatment surveillance. This review aims to summarize the changing epidemiology of colorectal cancer in young patients, overview existing screening guidelines, and discuss challenges and opportunities surrounding prevention of early-onset colon cancer. Full article