Search Results (152)

Ensuring the quality, reliability, and efficiency of cold chain logistics for thermolabile pharmaceutical products, particularly vaccines, remains a critical challenge in global health supply chains. These biologics require stringent temperature control throughout storage, transport, and distribution to preserve their efficacy. Persistent issues such as maintaining product integrity, accurately forecasting vaccine demand, and fostering trust among stakeholders often result in inefficiencies, waste, and public mistrust. This study proposes an intelligent digital management framework specifically designed for vaccine cold chains, integrating blockchain, the Internet of Things (IoT), and machine learning (ML) to address these challenges in a holistic and sustainable manner. The main innovation of the study lies in combining secure traceability, real-time cold chain monitoring, and predictive decision support within a unified vaccine cold chain management framework rather than treating these functions as isolated technological solutions. Using WHO immunization coverage data and vaccine-related review data, the framework supports vaccine demand forecasting through the Informer model and stakeholder trust assessment through BERT-based sentiment analysis. In the sentiment analysis task, the BERT model achieved ~80% accuracy on dominant sentiment classes, with a weighted F1-score of 0.6974, demonstrating strong performance on imbalanced datasets. By minimizing vaccine spoilage and enabling more accurate demand planning, the system reduces excess production and distribution, thus lowering resource consumption, carbon emissions, and financial waste. Moreover, trust-informed analytics support better alignment of supply with actual community needs, fostering equity and resilience in vaccine distribution. While this framework has been validated through simulations and experimental evaluation, further real-world testing is needed to assess long-term stability and stakeholder adoption. Nonetheless, it provides a scalable and adaptive foundation for advancing sustainability and transparency in pharmaceutical cold chains. Full article

The mining industry is among the most energy-intensive sectors and remains highly dependent on fossil fuels, particularly in remote, cold-climate regions where access to centralized electricity grids is limited. This dependence poses significant challenges in terms of operating costs, energy security, and greenhouse gas (GHG) emissions. This review provides a system-level analysis of energy consumption patterns, decarbonization pathways, and renewable energy integration strategies in the mining sector. The paper first examines the structure and drivers of energy demand in open-pit and underground mines, identifying transport systems, material handling, ventilation, and comminution processes as major energy consumers. It then analyzes technological and operational decarbonization strategies, including electrification, hybrid energy systems, renewable generation, and energy storage solutions. Particular attention is given to the technical constraints associated with site isolation, extreme climatic conditions, intermittency of renewable energy sources, and mine-life considerations. Case studies from the Canadian mining industry illustrate practical implementation challenges and achievable performance improvements. The analysis shows that while renewable energy technologies and storage systems are increasingly cost-competitive, deep decarbonization of mining operations requires integrated energy management, long-duration storage solutions, and site-specific hybrid system design. The review highlights engineering and strategic pathways that can progressively reduce fossil fuel dependence and support the transition toward low-carbon mining energy systems. Full article

Controlling carbon dioxide (CO₂) emissions remains a central challenge in Indonesia’s energy transition and its commitment to achieving net-zero emission targets. Carbon Capture and Storage (CCS) and Carbon Capture, Utilization, and Storage (CCUS) are widely recognized as important mitigation pathways, particularly for energy and industrial sectors where rapid decarbonization remains difficult. In parallel, cold plasma technology has emerged in the recent scientific literature as an early-stage, non-thermal approach for CO₂ activation under relatively low bulk temperature conditions, attracting interest as a potential long-term research pathway. This paper examines cold plasma technology within the broader CCS/CCUS landscape in Indonesia from a policy and technology perspective. The study adopts a qualitative and descriptive approach, synthesizing the selected academic literature on plasma-based CO₂ conversion, global CCUS development trends, and Indonesia’s regulatory, infrastructural, and energy system context. Rather than assessing techno-economic feasibility, the analysis focuses on identifying structural constraints, performance trade-offs, and policy-relevant considerations. The findings indicate that across plasma configurations, including dielectric barrier discharge, gliding arc, microwave, and radio frequency plasmas, current research outcomes remain constrained by low energy efficiency, limited scalability, and low technology readiness for large-scale applications. Reported performance metrics are largely derived from laboratory-scale studies under controlled conditions and cannot yet be extrapolated to real-world emission sources without a comprehensive system-level evaluation. Compared with established CCS and CCUS pathways, cold plasma technologies remain exploratory and lack the maturity required for near-term deployment. From a policy and research perspective, cold plasma should therefore be regarded as a long-term research option rather than an implementable mitigation solution for Indonesia, with its potential contribution lying in informing future research agendas, technology monitoring, and innovation planning, particularly in relation to CO₂ utilization concepts and decentralized energy systems, contingent upon significant advances in energy performance, system integration, and standardized evaluation frameworks. Full article

The pursuit of carbon neutrality demands advanced low-carbon energy processes and their effective integration into building systems. Ground-source heat pumps (GSHPs) offer a key pathway for decarbonizing heating, yet their cold-climate application is compromised by soil thermal imbalance, which degrades their long-term efficiency. This study proposes and evaluates an innovative air-assisted GSHP system that integrates a vegetable greenhouse with a zoned borehole configuration for seasonal thermal storage to achieve carbon neutrality. The system segregates boreholes into core and peripheral zones to establish a controlled soil temperature gradient, enabling cascaded heat storage and thermal optimization. A comprehensive year-long field test was conducted on a residential building in Harbin, China. The results demonstrate that the system reliably maintains comfortable indoor conditions during severe winters, achieving average seasonal COPs of 3.82 for the heat pump unit and 2.85 for the overall system. The zoned operation strategy successfully generated a significant intra-field soil temperature gradient, with a maximum differential of 5.9 °C between the core and peripheral boreholes during charging. The measured heat extraction-to-storage ratio was 0.598, confirming effective cascaded utilization. From an environmental perspective aligned with low-carbon energy technologies, the system achieves annual savings of 8.66 tons of standard coal and a net CO₂ reduction of 1.3 tons when accounting for regional grid carbon intensity. This research provides empirical validation and practical design guidance for implementing efficient GSHP systems in severely cold regions, thereby contributing substantively to building sector decarbonization. Full article

Background: Thermostability remains a key bottleneck for equitable access to mRNA-LNPs vaccines, mainly due to cold-chain requirements. Objectives and methods: Here, we optimized freeze-drying formulations by screening excipients (sugars, sugar alcohols, and proteins) and buffers to preserve mRNA-LNPs as solid formulations under ambient and refrigerated conditions. Physicochemical properties (size, polydispersity index [PDI], and encapsulation efficiency [EE]) and functional integrity, assessed by fluorescence-based in vitro transfection assays, were evaluated during long-term storage of up to six months. Results: Preliminary screening identified 20% sucrose and trehalose with Tris or histidine buffers as optimal for preserving physicochemical properties during freeze-drying, including high encapsulation efficiency (>90%), particle size (~200 nm), and low polydispersity (PDI < 0.2). Mannitol, gelatin, and PBS-based buffers showed adverse effects. At 4 °C, formulations F1–F3 maintained physicochemical stability and functional transfection activity for up to four months. In contrast, 20 °C storage caused progressive destabilization, with increased size, PDI, and encapsulation loss (>60% by six months). Among all formulations, 20% sucrose with 5 mM Tris (F1) showed the most robust preservation of physicochemical integrity and in vitro transfection efficiency under refrigerated and ambient conditions. Conclusions: Sugars outperformed sugar alcohols and gelatin as cryoprotectants. All formulations were stable, including functionally active at 4 °C for up to four months, while a sucrose/Tris formulation retained acceptable stability at 20 °C. Overall, the results demonstrate the feasibility of storing mRNA drug products as solid formulations at non-freezing temperatures. Full article

Injection-molding purges are heterogeneous, bulky residues whose uncertain composition and irregular geometry hinder direct reinsertion, making cold shredding costly and maintenance-intensive. This work develops a low-infrastructure solar-assisted pre-processing route using a PMMA Fresnel lens to induce controlled sub-onset softening and enable clean shear cutting without destructive thermal histories. The sub-onset softening is here defined into a viscoelastically active range (at or above T_g for the amorphous phase) while remaining below the melting onset (T_m, onset) and below the onset of thermal degradation (T_d, onset). The station was engineered via QFD and risk-oriented design tools, while a weighted Pugh matrix selected shear cutting over saw-based alternatives. A screening factorial DOE showed that lens height, angle, and their interaction significantly govern focal-spot diameter and receiver temperature, yielding linear relations for conservative set-point selection. Receiver benchmarking further indicated that copper reaches substantially higher temperatures than graphite under identical exposure conditions, supporting copper as the simplest, rapid-heating receiver. Under DOE-calibrated operation, tear-free shear cutting was achieved across representative purge families (PP–ABS, PC–ABS–PP, PA66, PA66-filler, and POM) without forced convection. From a recycling and waste-management perspective, the approach converts bulky purge scrap into mill-compatible feedstock with reduced mechanical resistance, lowering tool wear and fines generation, accelerating downsizing, and limiting stockpiling that elevates combustible-inventory fire risk. Overall, the proposed DOE-calibrated, operator-friendly framework improves recycling feasibility by enabling safer handling, more stable preprocessing throughput, and reduced reliance on disposal or long-term storage for heterogeneous industrial purges. Full article

Phase change materials (PCMs) have emerged as a key enabler of high-performance, low-carbon buildings through latent heat-based thermal energy storage. This paper presents a systematic and critical synthesis of advances in PCM technologies for building applications published between 2022 and 2025, analyzing over 300 peer-reviewed studies to evaluate thermal performance, economic viability, environmental impact, and climate adaptability across three integration approaches: passive, active, and hybrid systems. The studies analyzed show that passive envelope integration employing macroencapsulated or form-stable PCMs in walls, roofs, and glazing is reported to deliver 15–45% energy savings with payback periods of 8–15 years, primarily through enhanced thermal inertia and indoor temperature stabilization. Active systems, which couple PCMs with HVAC, heat pumps, or air handling units, are found to achieve 20–40% energy reductions and shorter payback periods (3–8 years) by enabling load shifting, peak shaving, and improved coefficient of performance (COP). Hybrid configurations integrating passive and active strategies with AI-driven control demonstrate, in the literature, the highest potential, with reported energy savings of up to 50%, though they entail greater complexity and capital cost. The review further highlights material-level innovations, including ternary composite PCMs, bio-based alternatives, and nano-enhanced formulations that address intrinsic limitations such as low thermal conductivity (0.1–0.3 W/m·K for organics) and cycling instability. Despite significant progress, critical gaps persist in standardized testing protocols, long-term field validation, comprehensive lifecycle assessments, and real-world scalability, particularly in tropical and cold climates. By bridging material science, building physics, and energy system engineering, this work provides a forward-looking roadmap to accelerate the deployment of PCM-based solutions in the global decarbonization of the built environment. Full article

Background/Objectives: Rapid diagnostic tests (RDTs) for human immunodeficiency virus (HIV) are widely used, but most kits lack standardized internal quality control (IQC) materials. In this study, we aimed to develop and evaluate a plasma-based IQC compatible with five HIV RDT brands and with proven long-term stability. Methods: Control samples at three reactivity levels were tested with five HIV RDT kits in lyophilized and liquid forms. Lyophilized samples were produced with and without trehalose, whereas liquid samples were prepared with and without StabilZyme™ SELECT Stabilizer (Stabilizer). Accelerated stability testing was performed at 37 °C and 45 °C for 28 days, and the most stable formulation was selected for long-term storage at 4 ± 2 °C and 25 ± 5 °C. Stability was assessed based on test-line visibility and signal intensity. Signal-intensity trends were analyzed using simple linear regression with a t-test on the slope; samples were considered stable when no significant trend was detected (p > 0.05). Results: Reactivity measured using the Elecsys HIV combi PT assay yielded cutoff index (COI) values of 772.65 (1:8) for the strong-positive control and 269.95 (1:25) for the weak-positive control. Trehalose-containing lyophilized samples maintained reactivity under accelerated testing at 37 and 45 °C and for 6 months at 4 ± 2 °C and 25 ± 5 °C, with no significant change in signal intensity (p > 0.05). Conclusions: The plasma-based IQC materials were compatible with all five HIV RDTs, and trehalose-stabilized lyophilized plasma showed high stability, supporting transport and storage without strict cold-chain requirements. Full article

Background: The long-term stability of mRNA-lipid nanoparticles (LNPs), essential for mRNA vaccines and gene therapies, relies on managing physicochemical properties to preserve their integrity and effectiveness through optimized formulation components. This study systematically evaluated LNP formulations with varied compositions, e.g., Dlin-MC3-DMA and ALC-0315 as ionizable lipids, and DMG-PEG2k or ALC-0159 as polyethylene glycol (PEG)-lipids, stored at −80 °C, −20 °C, 5 °C, and 25 °C in Tris buffer (pH 7.4) for 12 months. Methods: Sixteen quality attributes were analyzed, including particle size, mRNA encapsulation, lipid oxidation, and transfection efficiency over different formulations and storage temperatures to mechanistically evaluate the long-term stabilities. Results: Formulations stored at −80 °C and −20 °C retained acceptable stability, while storage at 5 °C caused aggregation, reduced in vivo expression, and mRNA degradation. Storage at 25 °C led to complete loss of transfection within six months. Mechanistic studies identified oxidative and hydrolytic lipid degradation (e.g., DSPC) in ALC-0315 formulations and MC3 N-oxidation with subvisible particulates in MC3-containing LNPs as primary failure modes. Increasing Tris buffer concentration accelerated 5′-cap hydrolysis, emphasizing the importance of a low-ionic-strength buffer for LNP formulations. Conclusions: Findings re-emphasize the necessity of deep-cold storage (≤−20 °C) and optimized formulation components to preserve mRNA–LNP integrity, offering insights for designing next-generation LNPs with improved shelf-life. Full article

Potato tubers can be dormant for 1–15 weeks after harvest, and the germination of tuber eye-buds is suppressed by cold. Genetic mechanisms regulating bud dormancy at low temperatures are not completely understood. We performed RNA-seq to compare gene expression in tubers after 0.5, 3.5, and 6.5 months of cold storage. Differentially expressed genes (DEGs) in non-dormant (3.5/6.5 months) vs. dormant (0.5 months) tubers were associated with transcriptional and translational activation, cell growth, metabolism, hormonal signaling, meristem development, dormancy break, and reproduction, confirming the non-dormant state of the meristem in the middle of storage. DEGs encoding transcription factors (TFs) (CBF1, FLC, SVP, HY5, GI, CO, FT, SOC1, CDF1, POTM1) were associated with the regulation of dormancy, flowering, and tuberization. TF DEGs upregulated (78) or downregulated (224) over four times in non-dormant vs. dormant tubers are considered potential coordinators of the endo- to non-dormancy transition and upcoming tuber sprouting during cold storage. RT-qPCR analysis of non-dormant and germinating buds revealed the upregulation of tuberigens (StSP3D, StFTL1-1, StFTL1-2), anti-tuberigens (StSP5G-like), and TF genes positively associated with tuberization or flowering/germination (StFDL1, StFDL, StCDF1, StCO-like). Our results should further investigation of the mechanisms underlying tuber meristem dormancy release and sprouting during long-term cold storage. Full article

Esteya vermicola is a nematophagous fungus with strong parasitic ability against the pinewood nematode (Bursaphelenchus xylophilus) and shows great potential for the biological control of pine wilt disease. However, this fungus is highly sensitive to environmental stress factors and often exhibits early necrosis when cultured on conventional nutrient-rich media, limiting its large-scale application. In this study, we optimized the long-term cultivation and conidiation conditions of E. vermicola CBS115803 by supplementing minimal medium (MM) with amino acids, and evaluated its stress tolerance and infectivity against the pinewood nematode. Among 20 tested amino acids, histidine significantly increased total conidia production, while arginine, glutamine, and proline markedly promoted the formation of lunate conidia. The combination of arginine, histidine, glutamine, and proline (AHGP) produced the highest overall conidia yield and lunate conidia proportion. The MM + AHGP medium maintained long-term colony viability, whereas colonies on PDA and CM media showed obvious degeneration. This formulation also improved mycelial growth, total conidiation, and the proportion of lunate conidia. Moreover, conidia produced on MM + AHGP exhibited the highest germination rates and infectivity under various stress conditions, including cold, heat, oxidative, osmotic, and UV stresses. Conidia germination was significantly enhanced following treatment at 0 °C, suggesting that low temperatures may activate dormancy-breaking pathways. This amino acid-optimized medium offers an effective technical foundation for stable large-scale production and storage of E. vermicola conidia, providing a new avenue for the biocontrol of pine wilt disease. Full article

Kidney transplantation remains the ultimate treatment option for patients with end-stage renal disease. However, the global shortage in donor kidneys, exacerbated by challenges such as ischemia–reperfusion injury (IRI), reduces renal graft viability and negatively impacts post-transplant outcomes. Static cold storage, the gold standard of organ preservation, reduces metabolic demand but increases the risk of cold-induced mitochondrial dysfunction and IRI, especially in marginal kidneys. The introduction of machine perfusion techniques allows renal grafts and other solid organ grafts to be preserved at a wider range of temperatures. Organ preservation temperatures play an important role in determining post-transplant outcomes in the transplantation of the kidney and other transplantable solid organs. Therefore, determining the optimal preservation temperature may help increase organ utilization by avoiding unnecessary graft discards and increasing the safe use of marginal organs. This review discusses the impact of various preservation temperatures and methods of preservation on post-transplant outcomes in renal grafts and other organ grafts. Drawing from preclinical, clinical, and meta-analytic studies, we compare hypothermic (0–4 °C), moderate hypothermic (10 °C), subnormothermic (20–32 °C), normothermic (35–37 °C), and subzero preservation strategies, and cellular and molecular changes that occur in renal grafts and other solid organ grafts during preservation at these temperatures. Overall, temperature-controlled machine perfusion outperforms static preservation of renal grafts and other solid organ grafts from marginal and deceased donors, potentially expanding donor pools and improving long-term graft survival, and suggests the need for future research to determine optimal preservation temperature for renal grafts and other solid organ grafts to improve viability and post-transplant outcomes. Full article

Accurate, real-time monitoring of meat freshness is essential for reducing food waste and safeguarding consumer health, yet conventional methods rely on costly, laboratory-grade spectroscopy or destructive analyses. This work presents a low-cost electronic-nose platform that integrates a compact array of metal-oxide gas sensors (Figaro TGS2602, TGS2603, and Sensirion SGP30) with a Gaussian Process Regression (GPR) model to estimate a continuous freshness index under refrigerated storage. The pipeline includes headspace sensing, baseline normalization and smoothing, history-window feature construction, and probabilistic prediction with uncertainty. Using factorial analysis and response-surface optimization, we identify history length and sampling interval as key design variables; longer temporal windows and faster sampling consistently improve accuracy and stability. The optimized configuration (≈143-min history, ≈3-min sampling) reduces mean absolute error from ~0.51 to ~0.05 on the normalized freshness scale and shifts the error distribution within specification limits, with marked gains in process capability and yield. Although it does not match the analytical precision or long-term robustness of spectrometric approaches, the proposed system offers an interpretable and energy-efficient option for short-term, laboratory-scale monitoring under controlled refrigeration conditions. By enabling probabilistic freshness estimation from low-cost sensors, this GPR-driven e-nose demonstrates a proof-of-concept pathway that could, after further validation under realistic cyclic loads and operational disturbances, support more sustainable meat management in future smart refrigeration and cold-chain applications. This study should be regarded as a methodological, laboratory-scale proof-of-concept that does not demonstrate real-world performance or operational deployment. The technical implications described herein are hypothetical and require extensive validation under realistic refrigeration conditions. Full article

Cold-pressed chia oil (Salvia hispanica L.) is highly susceptible to oxidative deterioration due to its exceptional α-linolenic acid content. This study evaluated the effect of increasing α-tocopherol concentrations (0–0.10% w/w) on its oxidative stability through accelerated oxidation testing (Oxitest) and long-term refrigerated storage. α-Tocopherol was selected because it is a widely accepted antioxidant in edible oils according to the Codex Alimentarius and FAO/WHO guidelines. A randomized block design (n = 3 independent extraction batches) was used to determine the induction period (IP) at 80 °C, followed by a 15-month evaluation at 15 °C of the control and the most promising treatment. α-Tocopherol increased oxidative resistance in a dose-dependent manner, but concentrations above 0.05% offered no additional benefits. The 0.05% treatment significantly prolonged the IP and effectively limited increases in peroxide and acidity values, keeping all parameters within Ecuadorian regulatory limits and consistent with international quality standards. Fatty-acid profiling confirmed that this antioxidant level slowed α-linolenic acid degradation, preserving the PUFA-rich profile of chia oil. These findings show that low-level α-tocopherol supplementation is a practical strategy to improve long-term stability of cold-pressed chia oil without altering its nutritional properties, providing valuable evidence for the formulation and commercialization of premium functional oils. Full article

In vitro germplasm conservation provides an alternative method for preserving plant species that are vulnerable to natural hazards or for which in situ conservation is costly and challenging to manage. This review examines the significance and challenges associated with various in vitro conservation methods. It also provides an overview of the current advances in cryopreservation technology for oil palm. In vitro conservation approaches include two strategies: medium-term conservation, in which plants are maintained through the slow growth of explants, facilitated by the gradual release of nutrients, and low-temperature storage. The second approach involves long-term preservation via cryopreservation in liquid nitrogen. Cryopreservation enables the storage of pollen, calli, somatic embryos, and zygotic embryos. Significant progress has been made in cryopreservation, which was initially limited to cold-tolerant species. New techniques focus on conserving sensitive species, such as oil palm, through rapid dehydration and vitrification procedures using various plant materials, particularly polyembryoids and zygotic embryos. Additionally, hardening of plant material is to be induced through pre-culture techniques to enhance their survival under osmotic stress and ultralow temperature. The mechanisms underlying the adaptability of various plant materials, i.e., somatic embryoids and zygotic embryos under cryopreservation, need to be understood. Full article