Search Results (3,479)

Trifolium repens L. (white clover) is a widely distributed perennial legume, which is regarded as one of the most important forages for its high protein content and excellent palatability. Low temperature limits the distribution and productivity of white clover, thereby reducing its economic returns. WRKY transcription factors are key regulators in stress defense and are involved in multiple abiotic stress responses in plants. In this study, a cold inducible gene named TrWRKY41 was cloned from white clover. The TrWRKY41 protein is predominantly localized in the nucleus and functions as a hydrophilic, acidic protein. Under cold stress, the overexpression plants had significantly higher chlorophyll (CHL) and proline (Pro) contents, significantly increased activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), and malondialdehyde (MDA) content significantly decreased. Compared to wild-type Arabidopsis thaliana, TrWRKY41-overexpressing plants exhibited better cold tolerance. In addition, target genes downstream of the TrWRKY41 transcription factor were predicted utilizing BLAST alignment and AlphaFold2 (version 0.2.0) software, the expression of six genes, including AtCOR47, AtCOR6.6, and AtABI5, was significantly up-regulated under cold stress. It suggests that TrWRKY41 may enhance cold tolerance in Arabidopsis by activating the ICE-CBF-COR cascade. This study provides candidate genes for research on enhancing the cold tolerance of white clover. Full article

Digestive enzymes play a crucial role in carbohydrate hydrolysis and subsequent glucose absorption, and their inhibition can contribute to improved glycemic regulation. Legumes, with their inherent enzyme-inhibitory properties, offer a natural approach for achieving this. In this study, accessions of M. uniflorum (Lam.) Verdc, an underutilized legume, were evaluated in vitro for their α-amylase and α-glucosidase inhibitory activities, as well as their protein, amylose, and resistant starch contents. The results revealed significant variation among the accessions. PI 174827 01 SD (IC₅₀ = 23.29 ± 0.01 µg/mL) and PI 173901 01 SD (IC₅₀ = 24.60 ± 0.01 µg/mL) demonstrated strong inhibition of α-amylase and α-glucosidase, respectively. Protein content ranged from 13.81 to 27.08%w/w d.w., with PI 180437 01 SD showing the highest percentage. Total starch content ranged from 27.48 to 54.70%w/w d.w., amylose from 27.05 to 48.13%w/w d.w., and resistant starch from 5.89% to 7.09%w/w d.w., with PI 174827 01 SD exhibiting both higher amylose and resistant starch contents. These findings suggest that M. uniflorum accessions possess enzyme-inhibitory and nutritional components that could be harnessed to develop functional foods, nutraceuticals, and pharmaceuticals for the management of diabetes and obesity. Full article

To clarify the effects of hydrogen peroxide (H₂O₂) seed soaking on the germination and stress resistance of different edible bean seeds, seeds of mung bean (Vigna radiata L. ‘Keda Green No. 2’), cowpea (Vigna unguiculata L. ‘Keda Cowpea No. 1’), and red bean (Vigna umbellata Thunb. ‘Jihong 352’) were soaked in a 50 mmol/L H₂O₂ solution. The study examined the germination and growth-related physiological indices of seeds after soaking. The results showed that hydrogen-peroxide-primed seeds of mung bean (GBH), cowpea (CBH), and red bean (RBH) exhibited significant improvements in germination performance and physiological activity compared with their respective controls (GBCK, CBCK, and RBCK). The results indicated that H₂O₂ soaking significantly improved the germination ability of the seeds, with the germination rate of mung beans, cowpeas, and red beans increasing by 48.89%, 21.11%, and 18.89%, respectively, and the germination percentage increasing by 31.11%, 24.45%, and 17.77%. Additionally, H₂O₂ soaking enhanced the activity of α-amylase, protease, and the antioxidant enzymes peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT); increased the soluble sugar and soluble protein content in the seeds; and reduced the malondialdehyde (MDA) content. The experiment demonstrated that H₂O₂ promotes the germination of mung bean, cowpea, and red bean seeds by influencing antioxidant enzyme activity, the breakdown of storage substances, and the regulation of germination-related substances, thereby improving seedling adaptation to environmental stress. This study aims to improve the germination rate of legume seeds using H₂O₂ treatment, providing a theoretical basis for techniques to enhance seed vigor, especially for seeds that perform poorly in germination under normal conditions. Full article

Background/Objectives: The global syndemic of obesity, undernutrition, and climate change highlight the complex health and environmental challenges faced by young adults. These challenges may intensify during the transition to university. As a matter of fact, limited budgets, time constraints, and insufficient culinary skills often lead to unbalanced diets and increased risk of obesity. University cafeterias, serving large numbers of students, represent an ideal setting to promote healthier and more sustainable eating behaviors. The FOOD-HACK Project aimed to design and implement a cafeteria-based intervention using nudging strategies to promote healthier and more sustainable lunch choices among university students. Methods: This pilot study employed a pre–post design with two independent phases in the Polo Cravino cafeteria at the University of Pavia. Food consumption was assessed over 12 non-consecutive days across four weeks. During the intervention, three nudging strategies were implemented: (1) choice architecture, (2) salient labeling, highlighting healthy and sustainable options, and (3) educational prompts. Results: Across both phases, 2400 tray photographs were collected. Post-intervention, the proportion of trays aligned with the Harvard Healthy Eating Plate and EAT-Lancet Planetary Diet models increased, reflecting higher consumption of vegetables and fruit. Legume-based first courses increased; however, legumes did not substantially replace animal proteins as the main protein source, and meat remained predominant in second courses. Reductions in trays containing multiple carbohydrate sources were also observed. Conclusions: The nudging intervention improved overall meal quality, demonstrating that subtle environmental modifications can guide students toward healthier dietary choices, particularly by increasing fruit and vegetable intake. However, the persistent preference for animal proteins highlights the challenge of shifting protein consumption toward more sustainable sources. These findings suggest that nudging can be an effective tool to promote healthier and more balanced eating behaviors in university settings, though complementary strategies may be needed to foster substantial changes in protein choices. Full article

The introduction of living mulches into an orchard can be considered an agroecological practice that can provide several ecosystem services related to integrated crop protection, also in relation to the impact on soil microbiome. In this study, the introduction in an organic apple orchard of two plant mixtures designed as multifunctional living mulches to reduce weed competition and increase shelter for beneficial arthropods was evaluated in relation to their impact on soil nutrient content and bacterial activity indices. One mixture was composed of Trifolium repens (20%) and Festuca ovina (80%), the second made of 40 different plant species including legumes, flowering species and grasses. Both living mulches increased N-nitrate levels in spring, and the two-component plant mixture was also able to improve P and K levels in soil at the same time, in comparison to the natural cover (control). The two mixtures induced an increase in bacterial activity in the beginning (40 plant species mix) or middle of the growing season (two-component plant mix), without major effects on bacterial biodiversity at the phyla level, showing a high share of Proteobacteria and Actinobacteriota among treatments. Nevertheless, both plant mixtures modified the phenotypic profile of the bacterial population, measured with the Biolog method, of different classes of C sources including carbohydrates, amino acids and carboxylic acid. The results are pointing to possible benefits of the practice on soil microbial activity, which will have to be confirmed by longer studies. Full article

Background: Dietary fiber plays a crucial role in body weight regulation and metabolic health. Despite benefits, fiber intake remains suboptimal. This study investigated the relationship between dietary fiber intake, BMI, and fiber sources among young Austrian adults in higher education. Methods: A cross-sectional study was conducted using a validated screening tool (18-item FiberScreen) to assess total daily fiber intake. Self-reported anthropometric data were used to calculate BMI, and participants were categorized into normal-weight and overweight groups. Differences in fiber intake and sources were analyzed by sex and BMI category. Correlations between fiber intake, BMI, and food group contributions were assessed. Results: The mean daily fiber intake among participants (n = 813) was 15.72 g/day. Overweight individuals consumed significantly less total fiber (13.18 ± 0.44 g/day) compared with those of normal weight (16.09 ± 0.22 g/day). Normal-weight participants obtained fiber mainly from fruits, vegetables, and legumes, while the overweight group relied more on potatoes and white bread. Fiber intake was also negatively correlated with BMI (p < 0.001). Conclusions: Low fiber intake is widespread among young adults and associated with higher BMI. The findings suggest that not only the quantity but also the quality of fiber sources play a role in weight status. Interventions aimed at improving dietary fiber intake through targeted education and healthier food environments would be a good strategy to support better health outcomes in young adults. Full article

This study explores the impact of shelterbelt forest plantations on agricultural productivity in Ukraine. The purpose of this article is to investigate how forest belts and land use patterns affect crop yields and agricultural land use in Ukraine, and to compare these patterns with factors contributing to forest cover loss in EU countries in order to develop practical management recommendations. Using geoinformation modeling and correlation analysis, we examined the relationship between shelterbelt coverage and agricultural indicators, including land leasing, crop yields and the planted area under annual and biennial crops. The total area of agricultural land protected by these plantations amounted to 51.66 thousand hectares, generating an additional 206.64 thousand centners of grain annually. Given the average price of 12.23 euros per centner for cereals and legumes, the total economic effect was estimated at approximately 2.53 million euros per year. The study also presents theoretical and methodological approaches for mathematically modeling economic indicators of forestry land use, drawing on successful practices from the European Union regarding sustainable development under significant anthropogenic, economic, and climatic pressures. The results highlight that shelterbelt plantations, once established, are among the most cost-effective agronomic practices, offering long-term environmental and economic benefits for sustainable agricultural development. Full article

This study evaluated the individual and combined effects of different bio-inputs—traditional filter cake, filter cake composted with ash, and a microbial inoculant—on the growth and physiological performance of Mucuna pruriens cultivated in soil contaminated with the herbicide tebuthiuron. The experiment followed a completely randomized design with twelve treatments and five evaluation periods (7, 21, 35, 49, and 70 days after sowing). Morphophysiological variables such as plant height, root length, dry biomass, and chlorophyll content were assessed. The results showed that the addition of traditional filter cake promoted significant growth in tebuthiuron-contaminated soil, while, in uncontaminated conditions, both organic residues and the microbial inoculant enhanced plant development, particularly at later stages. Initial phytotoxicity was observed in treatments with organic residues (up to 67% of samples before 35 days), but these effects decreased over time. The microbial inoculant performed better in the absence of organic amendments, suggesting possible antagonistic interactions. Tebuthiuron reduced chlorophyll content by inhibiting photosystem II, but this effect was mitigated by the addition of filter cake. Overall, the findings highlight the potential of integrating Mucuna pruriens cultivation with organic residues and microbial inoculants as an effective phytomanagement strategy for tebuthiuron-affected soils. This approach provides a sustainable model for improving soil health, supporting legume-based rehabilitation, and advancing biological alternatives to conventional remediation practices. Full article

Alfalfa (Medicago sativa L.), a drought-tolerant legume, significantly influences carbon and nitrogen cycling in arid and semi-arid regions. This study investigated carbon and nitrogen storage and sequestration dynamics in alfalfa pastureland cultivated for 2–7 years under temperate continental arid climate conditions (110–190 mm annual precipitation). Overall, the biomass, carbon and nitrogen sequestration in alfalfa pasture, and carbon and nitrogen storage and sequestration in soil exhibited a quadratic pattern with planting years. The above-ground biomass peaked at 19.28 t·hm⁻², with carbon and nitrogen sequestration reaching the highest level at 10.18 t·hm⁻² and 0.511 t·hm⁻², respectively, in year 5. Both annual carbon and nitrogen sequestration of the below-ground vegetation exhibited an increase, reaching a peak before decreasing with planting year, and from Y3 to Y7, the sequestration values were consistently higher than those in Y2. Soil carbon and nitrogen sequestration peaked in year 3. Compared to the adjacent fallow lands, alfalfa pasturelands maintained positive soil carbon sequestration until year 6 but became negative (−8.03 t·hm⁻²) by year 7. From years 2–6, alfalfa pasture fixed carbon and nitrogen at comparable rates but returned disproportionately less carbon than nitrogen to the soil. To optimize sustainability, we recommend (1) rotating alfalfa after 6 years to prevent soil nutrient depletion and (2) applying carbon-rich fertilizers post-year 3 to balance nutrients and prolong productivity in arid climates. Full article

Background/Objectives: Type 2 diabetes mellitus (T2DM) represents a growing public health burden in the European Union (EU), largely driven by modifiable lifestyle factors such as diet. This systematic review aimed to synthesize observational evidence on the associations between nutritional exposures and incident T2DM across the EU-28, with a focus on regional heterogeneity and implications for EU-level nutrition policy. Methods: The review followed PRISMA 2020 guidelines and was registered in PROSPERO (CRD42020219994). Searches were conducted in different databases (PubMed, Embase, Scopus, Web of Science) identifying 23,437 records, from which 104 observational studies were included. Eligible studies involved adults (≥18 years) without T2DM at baseline and assessed dietary exposures in relation to T2DM incidence. Data extraction and methodological quality assessment were performed in duplicate using the NHLBI tool in Covidence. Results: Most included studies were cohort studies (77.9%), primarily conducted in Western, Northern, and Southern Europe. Diets characterized by high consumption of whole and minimally processed foods, such as fruits, vegetables, legumes, whole grains, and fermented dairy, consistently showed associations with lower T2DM risk. In contrast, high intake of red and processed meats, sugar-sweetened beverages, and ultra-processed foods was linked to higher risk. Adherence to Mediterranean or plant-based dietary patterns was associated with lower risk, whereas high animal-protein dietary patterns were detrimental. Conclusions: Nutritional determinants play a decisive role in shaping T2DM risk in the EU. Evidence supports prioritizing dietary patterns rich in plant-based and minimally processed foods while reducing ultra-processed and meat-based products. Tailored region-specific policies are needed to address the T2DM epidemic and guide effective prevention strategies. Full article

Sustainability of animal production requires reducing reliance on soybean meal by identifying viable alternative protein sources. Within the framework of the Italian Agritech National Research Center, seven Italian research groups collaborated to evaluate unconventional feed ingredients and their effects on animal performance and product quality. Alternative legume seeds (peas, chickpeas, faba bean, and lupins) can partially or completely replace soybean meal without impairing productivity, while enhancing product health value and shelf-life through bioactive compounds. Microalgae (Chlorella, Spirulina) improved carotenoid content, antioxidant activity, fatty acid profile, and cholesterol levels in poultry products, with limited effects in pigs. Insects supported optimal growth in fish at 25–30% inclusion, whereas maximum recommended levels are 15% in broilers and 24% in laying hens to sustain growth, egg production, and quality. Camelina by-products are suitable for poultry diets at up to 5–10%, beyond which performance declines. Whole-plant soybean silage, tef (Eragrostis tef), and triticale–lupin intercropping represent promising protein-rich resources for ruminants, provided diets maintain balanced protein-to-energy ratios, adequate fibre characteristics, and appropriate harvest timing under drought-prone conditions. Collectively, these findings highlight the potential of diverse protein sources to improve the sustainability of livestock systems while preserving productivity and enhancing the nutritional quality of animal-derived foods. Full article

The integration of 3D printers into food production represents an unprecedented innovation, envisaging applications from the industry to missions in space to home cooking, with no geographical or sectoral limits. Extrusion food 3D printers are designed to use ‘food inks’ that must be produced from raw materials possessing a range of suitable characteristics (viscosity, elasticity, and others) that make them printable. Not all food matrices possess such characteristics, and additives are often needed to formulate food inks, which must also adapt to the complexity of the 3D model to be printed. Initially, mainly food matrices such as potatoes, chocolate, cereal, and legume flours and soluble-fiber-rich additives were tested with this new technology, with promising results. In recent years, alternative food matrices (e.g., based on insects, algae, cultured meat, and food waste) have begun to be experimented with, as 3D printing appears to be a suitable way to exploit their potential. This review aims to highlight recent studies that have investigated the development of innovative food ink formulations and trace a picture of the new food raw materials that are being tested for 3D food printing, the opportunities they represent, their nutritional properties, safety, and technological challenges. This review considered a total of 46 papers, selected from 330 papers published in the last 8 years (2018–2025) on the generic subject of 3D food printing. Full article

The present research aimed to design innovative wheat cultivation systems that are less resource-intensive, promote biodiversity, and show greater resilience to both biotic and abiotic stress. It was focused on the cultivation and characterization of two evolutionary populations (EPs) of common wheat, namely EP_Floriddia and EP_Li Rosi, grown in Italy, over two growing seasons. The EPs were cultivated in organic management under legume or wheat precessions. Physico-chemical analyses included thousand kernel weight (TKW), test weight (TW), and ash content. Location and genotype mostly influenced TKW; TW, instead, was affected only by year. Wholemeal flour from each sample was assessed for protein content (PC), total starch (TS), total antioxidant capacity (TAC), and total dietary fiber (TDF). Protein content was higher on leguminous precessions than on wheat; the opposite behavior was observed for TS. The growing season predominantly impacted on TAC and TDF values. Technological and rheological parameters such as alveograph W and P/L value, SDS sedimentation test, farinograph quality, gluten index, and falling number revealed EP_Li Rosi as the best for baking aptitude, although both EPs were characterized by weak gluten. These findings support the use of EPs under legume precession as an agroecological approach to pursue agrifood biodiversity, quality, and sustainability. Full article

Leguminous plants are critical global crops for food security, animal feed, and ecological sustainability due to their ability to establish nitrogen-fixing symbioses with rhizobia and their high nutritional value. Autophagy, a highly conserved eukaryotic catabolic process, mediates the degradation and recycling of cytoplasmic components through the fusion of autophagosome with vacuole/lysosome and plays essential roles in plant growth, stress adaptation, and cellular homeostasis. This review systematically summarizes current knowledge of autophagy in both Arabidopsis and leguminous plants. We first outline the conserved molecular machinery of autophagy, focusing on core autophagy-related (ATG) genes in Arabidopsis and key legume species such as Glycine max, Arachis hypogaea, Pisum sativum, Cicer arietinum, and Medicago truncatula. Furthermore, the review dissects the intricate molecular regulatory networks controlling autophagy, with an emphasis on the roles of phytohormones, transcription factors, and epigenetic modifications. We then highlight the multifaceted physiological functions of autophagy in these plants. Additionally, a preliminary analysis of the ATG8 gene family in peanut indicates that its members may be involved in seed development, biological nitrogen fixation, and drought resistance. Finally, it highlights key unresolved challenges in legume autophagy research and proposes future research directions. This review aims to provide a comprehensive theoretical framework for understanding the unique regulatory mechanisms of autophagy in legumes and to provide insights for molecular breeding aimed at developing stress-resilient, high-yielding, and high-quality legume cultivars. Full article

Effective implementation and optimization of organic amendments and other management practices is essential for sustainable organic vegetable production, yet needed information is lacking on the effects and benefits of different organic matter amendments and pest management approaches under Northeastern USA production conditions. The impacts of soil amendments of biochar or humus (soluble humate complex) in conjunction with compost, as well as the presence or absence of an early-season insect netting row cover (mesotunnels), were evaluated on soil chemical and biological properties, crop development and yield, and disease and pest issues in organic vegetable production, as represented by legume (green snap bean), cucurbit (green zucchini squash), and amaranth (garden beet) vegetable crops, in a three-year field trial in Maine. Composted cow manure and a commercial organic fertilizer alone were included as controls. All plots were either covered or not covered with a permeable insect netting row cover from the time of planting until flowering. All compost-based amendments increased soil pH; organic matter; microbial activity; crop yields; and K, Mg, and Ca content relative to a fertilizer-only treatment. Biochar amendments further increased soil pH, CEC, and Ca content above those of compost alone and also resulted in the overall highest yields of bean and zucchini but were not significantly greater than with compost amendment alone. Humus amendments did not improve soil characteristics, with some indications of potential reductions in emergence and yield. Insect netting substantially improved yield of zucchini (by 59%) and somewhat improved bean yield (by 11%), in addition to improving plant emergence and reducing insect leaf damage, but it did not reduce powdery mildew on zucchini or provide any significant benefits for beets. These results help define specific management practices to improve organic vegetable production and provide useful information and options for growers. Full article