Search Results (1,660)

Background and Objective: This study presents a combined osteometric and biomechanical analysis of a Korean female cadaver exhibiting bilateral lower limb bone asymmetry with abnormal curvature and callus formation on the left femoral midshaft. Methods: To investigate bilateral bone length differences, osteometric measurements were conducted at standardized landmarks. Additionally, we developed three gait models using Meta Motivo, an open-source reinforcement learning platform, to analyze how skeletal asymmetry influences stride dynamics and directional control. Results: Detailed measurements revealed that the left lower limb bones were consistently shorter and narrower than their right counterparts. The calculated lower limb lengths showed a bilateral discrepancy ranging from 39 mm to 42 mm—specifically a 6 mm difference in the femur, 33 mm in the tibia, and 36 mm in the fibula. In the gait pattern analysis, the normal model exhibited a straight-line gait without lateral deviation. In contrast, the unbalanced, non-learned model demonstrated compensatory overuse and increased stride length of the left lower limb and a tendency to veer leftward. The unbalanced, learned model showed partial gait normalization, characterized by reduced limb dominance and improved right stride, although directional control remained compromised. Conclusions: This integrative approach highlights the biomechanical consequences of lower limb bone discrepancy and demonstrates the utility of virtual agent-based modeling in elucidating compensatory gait adaptations. Full article

Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is eroding therapeutic options for urinary tract infections. We isolated a multidrug-resistant strain from the urine of a chronically bacteriuric patient and confirmed its identity by Vitek-2 and MALDI-TOF MS. Initial disk-diffusion profiling against 48 antibiotics revealed susceptibility to only 5 agents. One month later, repeat testing showed that tetracycline alone remained active, highlighting the strain’s rapidly evolving resistome. Given the scarcity of drug options, we performed an “aromatogram” with seven pure essential oils, propolis, and two commercial phytotherapeutic blends. Biomicin Forte^® produced a 30 mm bactericidal halo, while thyme, tea tree, laurel, and palmarosa oils yielded clear inhibition zones of 11–22 mm. These in vitro data demonstrate that carefully selected plant-derived products can target CR-Kp where conventional antibiotics fail. Integrating aromatogram results into One Health’s stewardship plans may therefore help preserve last-line antibiotics and provide adjunctive options for persistent urinary infections. Full article

The stability of shield tunnel segmental linings is highly sensitive to the lateral pressure coefficient, especially under weak, heterogeneous, and variable geological conditions. However, the mechanical behavior of steel plate-reinforced linings under such conditions remains insufficiently characterized. This study aims to investigate the effects of varying lateral pressures on the structural performance of reinforced tunnel linings. To achieve this, a custom-designed full-circumference loading and unloading self-balancing apparatus was developed for scaled-model testing of shield tunnels. The experimental methodology allowed for precise control of loading paths, enabling the simulation of realistic ground stress states and the assessment of internal force distribution, joint response, and load transfer mechanisms during the elastic stage of the structure. Results reveal that increased lateral pressure enhances the stiffness and bearing capacity of the reinforced lining. The presence and orientation of segment joints, as well as the bonding performance between epoxy resin and expansion bolts at the reinforcement interface, significantly influence stress redistribution in steel plate-reinforced zones. These findings not only deepen the understanding of tunnel behavior in complex geological environments but also offer practical guidance for optimizing reinforcement design and improving the durability and safety of shield tunnels. Full article

To enhance the anti-overturning performance of poles and prevent tilting or collapse, a prefabricated foundation for distribution lines is developed. Field tests are conducted on five groups of foundations. Based on the test results, finite element analysis (FEA) is employed to investigate the influence of different factors—such as pole embedment depth, foundation locations, soil type, and soil parameters—on the anti-overturning performance of pole prefabricated foundations. The results indicate that under ultimate load conditions, the reaction force distribution at the base of the foundation approximates a triangular pattern, and the lateral earth pressure on the pole follows an approximately quadratic parabolic distribution along the depth. When the foundation size increases from 0.8 m to 0.9 m, the bearing capacity of the prefabricated foundation improves by 8%. Furthermore, when the load direction changes from 0° to 45°, the foundation’s bearing capacity increases by 14%. When the foundation is buried at a depth of 1.0 m, compared with the ground position, the ultimate overturning moment of the prefabricated foundation increases by 10%. Based on field test results, finite element simulation results, and limit equilibrium theory, a calculation method for the anti-overturning bearing capacity of prefabricated pole foundations is developed, which can provide a practical reference for the engineering design of distribution line poles and their prefabricated foundations. Full article

Axial truncal dystonia can present as either flexion or extension, often with a tendency toward lateral movement. Flexion dystonia is more common and may represent a clinical spectrum associated with parkinsonism. In contrast, extensor trunk dystonia is less frequent and exhibits a diverse range of causes. In this paper, we reviewed the literature on axial extensor trunk dystonia. We identified 11 studies involving 49 patients, of which only 10 had idiopathic trunk dystonia. Treatment with botulinum neurotoxin A (BoNT/A) emerged as the most effective therapy; however, many studies did not provide detailed descriptions of the treatment (4/11) and follow-up periods were not specified or short term (up to one–two years). We present four new, well-documented patients with the idiopathic form of extensor trunk dystonia who were treated with BoNT/A with moderate to significant effect according to Global Clinical Impression scale (GCI) and Burke-Fahn-Marsden (BFM) dystonia scale. These cases include long-term follow-up for three patients, all without any adverse events. While the diagnostic process and treatment can be challenging, we recommend using BoNT/A with adjusted doses tailored to the appropriate muscle groups as a first-line treatment. Full article

The design and optimization of drive distribution strategies are critical for enhancing the performance of Formula Student electric racing cars, which face demanding operational conditions such as rapid acceleration, tight cornering, and variable track surfaces. Given the increasing complexity of racing environments and the need for adaptive control solutions, a multi-mode adaptive drive distribution strategy for four-wheel-drive Formula Student electric racing cars is proposed in this study to meet specialized operational demands. Based on the dynamic characteristics of standardized test scenarios (e.g., straight-line acceleration and figure-eight loop), two control modes are designed: slip-ratio-based anti-slip control for longitudinal dynamics and direct yaw moment control for lateral stability. A CarSim–Simulink co-simulation platform is established, with test scenarios conforming to competition standards, including variable road adhesion coefficients (μ is 0.3–0.9) and composite curves. Simulation results indicate that, compared to conventional PID control, the proposed strategy reduces the peak slip ratio to the optimal range of 18% during acceleration and enhances lateral stability in the figure-eight loop, maintaining the sideslip angle around −0.3°. These findings demonstrate the potential for significant improvements in both performance and safety, offering a scalable framework for future developments in racing vehicle control systems. Full article

Unbalanced accelerations occurring during tram travel have a significant impact on passenger comfort and safety, as well as on the rate of wear and tear on infrastructure and rolling stock. Ideally, these dynamic forces should be monitored continuously in real-time; however, traditional systems require high-precision accelerometers and proprietary software—investments often beyond the reach of municipally funded tram operators. To this end, as part of the research project “Accelerometer Measurements in Rail Passenger Transport Vehicles”, pilot measurement campaigns were conducted in Poland on tram lines in Gdańsk, Toruń, Bydgoszcz, and Olsztyn. Off-the-shelf smartphones equipped with MEMS accelerometers and GPS modules, running the Physics Toolbox Sensor Suite Pro app, were used. Although the research employs widely known methods, this paper addresses part of the gap in affordable real-time monitoring by demonstrating that, in the future, equipment equipped solely with consumer-grade MEMS accelerometers can deliver sufficiently accurate data in applications where high precision is not critical. This paper presents an analysis of a subset of results from the Gdańsk tram network. Lateral (x) and vertical (z) accelerations were recorded at three fixed points inside two tram models (Pesa 128NG Jazz Duo and Düwag N8C), while longitudinal accelerations were deliberately omitted at this stage due to their strong dependence on driver behavior. Raw data were exported as CSV files, processed and analyzed in R version 4.2.2, and then mapped spatially using ArcGIS cartograms. Vehicle speed was calculated both via the haversine formula—accounting for Earth’s curvature—and via a Cartesian approximation. Over the ~7 km route, both methods yielded virtually identical results, validating the simpler approach for short distances. Acceleration histograms approximated Gaussian distributions, with most values between 0.05 and 0.15 m/s², and extreme values approaching 1 m/s². The results demonstrate that low-cost mobile devices, after future calibration against certified accelerometers, can provide sufficiently rich data for ride-comfort assessment and show promise for cost-effective condition monitoring of both track and rolling stock. Future work will focus on optimizing the app’s data collection pipeline, refining standard-based analysis algorithms, and validating smartphone measurements against benchmark sensors. Full article

Hydraulic fracturing is a crucial technology for developing unconventional oil and gas resources. However, conventional geophysical methods struggle to efficiently and accurately image proppant-connected channels created by hydraulic fracturing. The borehole-to-surface electromagnetic imaging method (BSEM) overcomes this limitation by utilizing a controlled cased well source. Placing the source close to the target reservoir and deploying multi-component receivers on the surface enable high-precision lateral monitoring, providing an effective approach for dynamic monitoring of hydraulic fracturing operations. This study focuses on key aspects of forward modeling for BSEM. A three-dimensional finite-volume method based on the Yee grid was used to simulate the borehole-to-surface electromagnetic system incorporating metal casings, validating the method of simulating metal casing using multiple line sources. The simulation of the observation system and the frequency-domain electromagnetic monitoring simulation based on actual well data confirm BSEM’s high sensitivity for monitoring deep subsurface formations. Critically, well casing exerts a substantial influence on surface electromagnetic responses, while the electromagnetic contribution from line sources emulating perforation zones necessitates explicit incorporation within data processing workflows. Full article

This study aimed to investigate the tyrosine kinase inhibitor Nintedanib and its potential role in reversing epithelial–mesenchymal transition (EMT) induced by transforming growth factor beta 2 (TGF-β2) in retinal pigment epithelial (RPE) cells, along with its therapeutic potential using a mouse model of subretinal fibrosis. We hypothesized that the blockade of angiogenesis promoting and fibrosis inducing signaling using the receptor tyrosine kinase inhibitor Nintedanib (OfevTM) can prevent or reverse EMT both in vitro and in our in vivo model of subretinal fibrosis. Primary human retinal pigment epithelial cells (phRPE) and adult retinal pigment epithelial cell line (ARPE-19) cells were treated with TGF-β210 ng/mL for two days followed by four days of Nintedanib (1 µM) incubation. Epithelial and mesenchymal phenotypes were assessed by morphological examination, quantitative real-time polymerase chain reaction(qPCR) (ZO-1, Acta2, FN, and Vim), and immunocytochemistry (ZO-1, vimentin, fibronectin, and αSMA). Metabolites were measured using luciferase-based assays. Extracellular acidification and oxygen consumption rates were measured using the Seahorse XF system. Metabolic-related genes (GLUT1, HK2, PFKFB3, CS, LDHA, LDHB) were evaluated by qPCR. A model of subretinal fibrosis using the two-stage laser-induced method in C57BL/6J mice assessed Nintedanib’s therapeutic potential. Fibro-vascular lesions were examined 10 days later via fluorescence angiography and immunohistochemistry. Both primary and ARPE-19 RPE stimulated with TGF-β2 upregulated expression of fibronectin, αSMA, and vimentin, and downregulation of ZO-1, consistent with morphological changes (i.e., elongation). Glucose consumption, lactate production, and glycolytic reserve were significantly increased in TGF-β2-treated cells, with upregulation of glycolysis-related genes (GLUT1, HK2, PFKFB3, CS). Nintedanib treatment reversed TGF-β2-induced EMT signatures, down-regulated glycolytic-related genes, and normalized glycolysis. Nintedanib intravitreal injection significantly reduced collagen-1+ fibrotic lesion size and Isolectin B4+ neovascularization and reduced vascular leakage in the two-stage laser-induced model of subretinal fibrosis. Nintedanib can induce Mesenchymal-to-Epithelial Transition (MET) in RPE cells and reduce subretinal fibrosis through metabolic reprogramming. Nintedanib can therefore potentially be repurposed to treat retinal fibrosis. Full article

Nipah virus (NiV) and Hendra virus (HeV), which both belong to the genus henipavirus, are zoonotic pathogens that cause severe systemic, neurological, and/or respiratory disease in humans and a variety of mammals. Therefore, monitoring viral prevalence in natural reservoirs and rapidly diagnosing cases of henipavirus infection are critical to limiting the spread of these viruses. Current laboratory methods for detecting NiV and HeV include virus isolation, reverse transcription quantitative real-time PCR (RT-qPCR), and antigen detection via an enzyme-linked immunosorbent assay (ELISA), all of which require highly trained personnel and specialized equipment. Here, we describe the development of a point-of-care customized immunochromatographic lateral flow (ILF) assay that uses recombinant human ephrin B2 as a capture ligand on the test line and a NiV-specific monoclonal antibody (mAb) on the conjugate pad to detect NiV and HeV. The ILF assay detects NiV and HeV with a diagnostic specificity of 94.4% and has no cross-reactivity with other viruses. This rapid test may be suitable for field testing and in countries with limited laboratory resources. Full article

Background/Objective: Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) has affected more than 1700 women with textured breast implants. About 80% of patients present with fluid (seroma) around their implant. BIA-ALCL can be cured by surgery alone when confined to the seroma and lining of the peri-implant capsule. To address the need for early detection, we developed a rapid point of care (POC) lateral flow assay (LFA) to identify lymphoma in seromas. Methods: We compared 28 malignant seromas to 23 benign seromas using both ELISA and LFA. LFA test lines (TL) and control lines (CL) were visualized and measured with imaging software and the TL/CL ratio for each sample was calculated. Results: By visual exam, the sensitivity for detection of CA9 was 93% and specificity 78%, while the positive predictive value was 84% and negative predictive value 90%. Quantitative image analysis increased the positive predictive value to 96% while the negative predictive value reduced to 79%. Conclusions: We conclude that CA9 is a sensitive biomarker for detection and screening of patients for BIA-ALCL in patients who present with seromas of unknown etiology. The CA9 LFA can potentially replace ELISA, flow cytometry and other tests requiring specialized equipment, highly trained personnel, larger amounts of fluid and delay in diagnosis of BIA-ALCL. Full article

To investigate how severe wear at No. 12 turnout frogs in an urban rail transit line operating at speeds over 120 km/h on the dynamic performance of the vehicle, a vehicle–frog coupled dynamic model was established by employing the 2021 version of SIMPACK software. Profiles of No. 12 alloy steel frogs and metro wheel rims were measured to simulate wheel–rail interactions as the vehicle traverses the turnout, using both brand-new and worn frog conditions. The experimental results indicate that increased service life deepens frog wear, raises equivalent conicity, and intensifies wheel–rail forces. When a vehicle passes through the frog serviced for over 17 months at the speed of 120 km/h, the maximum derailment coefficient, lateral acceleration of the car body, and lateral and vertical wheel–rail forces increased by 0.14, 0.17 m/s², 9.52 kN, and 105.76 kN, respectively. The maximum contact patch area grew by 35.73%, while peak contact pressure rose by 236 MPa. To prevent dynamic indicators from exceeding safety thresholds and ensure train operational safety, it is recommended that the frog maintenance cycle be limited to 12 to 16 months. Full article

Aiming at the problem that the aggravation of the wheel tread wear of high-speed trains leads to the deterioration of train operation performance and an increase in re-profiling times, a multi-objective data-driven optimization design method for the wheel profile is proposed. Firstly, the chaotic map is introduced into the population initialization process of the golden jackal algorithm. In the later stage of the algorithm iteration, random disturbance is introduced with optimization algebra as the switching condition to obtain an improved optimization algorithm, and the performance index of the optimization algorithm is verified to be superior to other algorithms. Secondly, the improved multi-objective optimization algorithm and data-driven model are used to optimize the tread coordinates and obtain an optimized profile. The vehicle dynamics performance of the optimized profile and the wheel wear evolution after long-term service are compared. The results show that the tread wear index of the left and right wheels in a straight line is reduced by 62.4% and 62.6%, respectively, and the wear index of the left and right wheels in a curved line is reduced by 26.5% and 5.5%, respectively. The stability and curve passing performance of the optimized profile are improved. Under the long-term service conditions of the train, the wear amount of the optimized profile is greatly reduced. After the wear prediction of 200,000 km, the wear amount of the optimized profile is reduced by 60.1%, and it has better curve-passing performance. Full article

Rectangular box jacking is widely used in densely developed urban areas. However, when conducted with limited clear distance near existing metro tunnels, it introduces considerable structural safety risks. This study investigates a large-section rectangular box jacking project in Suzhou that crosses a double-line metro tunnel with minimal vertical clear distance. Integrated field monitoring and finite element simulations were conducted to analyze the tunnel’s deformation behavior during various jacking phases. The results show that the upline tunnel experienced greater uplift than the downline tunnel, with maximum vertical displacement occurring directly beneath the jacking axis. The affected zone extended approximately 20 m beyond the pipe gallery boundaries. Both the tunnel vault and ballast bed exhibited vertical uplift, while the hance displaced laterally toward the launching shaft. These deformations showed clear stage-dependent patterns strongly influenced by the relative position of the jacking machine. Numerical simulations demonstrated that doubling the pipe–tunnel clearance reduced the vault displacement by 58.87% (upline) and 51.95% (downline). Increasing the pipe–slurry friction coefficient from 0.1 to 0.3 caused the hance displacement difference to rise from 0.12 mm to 0.36 mm. Further sensitivity analysis reveals that when the jacking machine is positioned directly above the tunnel, grouting pressure is the greatest influence on the structural response and must be carefully controlled. The proposed methodology and findings offer valuable insights for future applications in similar tunnelling projects. Full article

Sarcomas are heterogeneous mesenchymal tumors, and their pharmacological treatment remains challenging due to the high toxicity and poor efficacy of current therapies. This study aimed to identify common overexpressed kinases in the four most frequent sarcoma subtypes to establish novel therapeutic targets. A bioinformatics approach using patient-derived gene expression data sets identified overexpressed kinases shared across these sarcoma types. Later, BUB1 was determined as the kinase consistently overexpressed across the osteosarcoma, liposarcoma, leiomyosarcoma, and synovial sarcoma. Moreover, the role of this kinase was further validated through molecular and functional assays, including pharmacological inhibition in cell lines derived from the four sarcoma subtypes. BUB1 inhibition reduced the phosphorylation of AKT and H2A proteins, precluded cell proliferation, and inhibited colony formation in sarcoma cells. Finally, overall survival analysis highlighted a strong correlation between high BUB1 expression and poorer survival rates in sarcoma patients. Altogether, these findings underscore the potential of BUB1 as a therapeutic target and prognostic marker in sarcomas. Targeted inhibition of BUB1 may provide a novel strategy to reduce tumor growth and improve outcomes for patients with bone and soft tissue sarcomas. Full article