Search Results (1,057)

Lentinula edodes (shiitake) is a major edible and medicinal mushroom and a key component of the horticultural mushroom industry in East Asia. During April–June 2024 cropping season, a widespread blue mold outbreak was observed on bag-cultivated shiitake in Xixia County, Henan Province, China. Affected cultivation rooms showed extensive blue-green sporulation on the exposed surfaces of substrate blocks and on developing and mature fruiting bodies, leading to rapid loss of marketability. To clarify the etiology of this disease, we coupled field surveys with morphological, molecular, and pathogenicity analyses. Fifty-five Penicillium isolates were obtained from symptomatic cultivation bags. Three representative isolates (LE06, LE15, and LE26) were characterized in detail. Colonies on PDA produced velutinous to floccose mycelia with blue-green conidial masses and terverticillate penicilli bearing smooth-walled, globose conidia. Sequencing of four loci—the internal transcribed spacer (ITS1-5.8S-ITS2), β-tubulin (benA), calmodulin gene (CaM), and RNA polymerase II second largest subunit (rpb2)—followed by multilocus phylogenetic analysis placed all three isolates in a well-supported clade with the ex-type CBS 227.28 of Penicillium bialowiezense. Inoculation of healthy shiitake cultivation bags with conidial suspensions (1 × 10⁶ conidia mL⁻¹) reproduced typical blue mold symptoms on substrate surfaces and fruiting bodies within 40 days post inoculation, whereas mock-inoculated controls remained symptomless. The pathogen was consistently reisolated from diseased tissues and showed identical ITS and benA sequences to the inoculated strains, thereby fulfilling Koch’s postulates. This is the first confirmed report of P. bialowiezense causing blue mold on shiitake, and it expands the known host range of this species. Our findings highlight the vulnerability of bag cultivation systems to airborne Penicillium contaminants and underscore the need for improved hygiene, environmental management, and targeted diagnostics in commercial shiitake production. Full article

Ganoderma is a genus of medically significant fungi, that is used in traditional medicine and is increasingly incorporated into modern nutraceuticals and pharmaceuticals. Accurate species identification and product standardization remain major challenges due to morphological plasticity and cryptic diversity. This review articulates current advances in Ganoderma systematics, phylogenetics, and metabolomics, with an emphasis on molecular identification strategies and chemical profiling. Internal transcribed spacer (ITS) sequencing has substantially improved species delineation compared with morphology alone, but its resolving power is limited in closely related species complexes, necessitating complementary multilocus approaches. Advances in metabolomics, and LC-MS- and HPLC-based profiling of triterpenes and polysaccharides, have enhanced species discrimination, chemotaxonomic resolution, and quality control of commercial products. Integrating molecular barcoding with metabolomic fingerprints provides a more robust framework for classification, pharmacological evaluation, and standardization. This review also highlights significant geographic knowledge gaps, particularly in Africa, where molecular and metabolomic data remain scarce despite high species diversity. Full article

Sporotrichosis, the most common implantation mycosis worldwide, is caused by dimorphic fungi of several species of the genus Sporothrix. Sporothrix brasiliensis, the most virulent species, has emerged in Latin America as an epi-zoonotic pathogen linked to domestic cats, dogs and humans. This report describes a confirmed human case of lymphocutaneous sporotrichosis caused by S. brasiliensis in Chile, associated with feline exposure in a veterinarian. Diagnosis was supported by morphological and molecular analyses of the internal transcribed spacer (ITS) and β-tubulin gene. The patient responded favorably to itraconazole therapy. This case highlights the growing relevance of S. brasiliensis in Chile and reinforces the need for integrated One Health surveillance strategies. Full article

Concrete is the most widely used construction material worldwide, yet its production and disposal pose significant environmental challenges due to high carbon emissions and limited recyclability. While microbial colonization of concrete is often associated with structural deterioration, recent research has highlighted the potential of microorganisms to contribute positively to concrete recycling and self-healing. In this study, we investigated the bacterial and fungal communities inhabiting urban concrete samples using amplicon-based taxonomic profiling targeting the 16S rRNA gene and internal transcribed spacer (ITS) region. Our analyses revealed a diverse assemblage of microbial taxa capable of surviving the extreme physicochemical conditions of concrete. Several taxa were associated with known metabolic functions relevant to concrete degradation, such as acid and sulphate production, as well as biomineralization processes that may support crack repair and surface sealing. These findings suggest that concrete-associated microbiomes may serve as a reservoir of biological functions with potential applications in sustainable construction, including targeted biodegradation for recycling and biogenic mineral formation for structural healing. This work provides a foundation for developing microbial solutions to reduce the environmental footprint of concrete infrastructure. Full article

Isousnic acid (isoUA) has been detected in a few usnic acid (UA)-producing lichens with chemotaxonomic values. IsoUA was first isolated from a specimen belonging to Cladonia arbuscula s.l. (referred to as C. mitis in the publication). However, the isolation and detection of isoUA in this Cladonia species have not been reproduced and confirmed with clear evidence. This study focused on C. arbuscula s.l. collected in Iceland and aimed to (1) identify the lichen specimen using DNA barcoding and (2) investigate whether isoUA is produced using a series of chromatographic methods. The fungal nuclear ribosomal internal transcribed spacer (nrITS) barcode was sequenced, and the specimen was identified as C. arbuscula, following recent circumscription recommendations. Routine metabolite profiling did not detect isoUA, and it could only be identified after vigorous chromatographic purification and concentration steps using flash chromatography and preparative high-performance liquid chromatography. IsoUA was found in trace quantities (~24 µg/g dry weight), which likely explains its absence in routine metabolite profiling. A rapid ultra-high-performance liquid chromatography (UHPLC) method using a pentafluorophenyl column was developed to separate UA and isoUA. Our study highlights the importance of an integrative approach combining DNA barcoding and detailed chromatographic analyses for lichen chemistry research. Full article

The genus Satyrium (Orchidaceae) is a large, mostly sub-Saharan genus with a single species reported from Madagascar and Asia. Taxonomical complexity and high morphological diversity make the classification within the genus difficult to handle. In this study, we attempted to solve this problem using a comprehensive approach based on data from multiple sources. We combined morphological data from vegetative parts with data on flower structure using timescale phylogenetics conducted for both nuclear internal transcribed spacer (ITS) and plastid markers (matK, trnS-trnG, trnL, trnL-trnF). Phylogenetic studies confirmed most of the results of previous studies and led to the identification of six potential hybridization events within the genus. Morphological diversity often does not correspond to phylogenetic relationships within the genus, and many evolutionary lineages began to diverge only at the end of the early Miocene and in the late Miocene. The development of similar characteristics is the result of this diversification under the influence of similar environmental pressures. Reconstruction of the historical geographical range of Satyrium showed that the regions of South Africa and the mountainous areas of Eastern Africa played the most important role in the diversification of the genus. Full article

Cornus officinalis is a valuable traditional Chinese medicinal (TCM) plant species with both therapeutic and ornamental attributes. It is widely used in TCM prescriptions to nourish the liver and kidneys and constitutes a critical component of numerous classical formulas. In recent years, the large-scale cultivation of this medicinal plant has been expanded in Xixia County, Henan Province, China. Field investigations have revealed widespread brown leaf spot, accompanied by reductions in yield and quality. In this study, symptomatic leaves were collected for pathogen isolation. Tissue isolations consistently yielded a Colletotrichum fungus, and morphology combined with multi-locus phylogenetic analyses (the internal transcribed spacer, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase, actin, and β-tubulin) identified the pathogen as Colletotrichum siamense. Pathogenicity assays (conducted by either wounding and inoculating detached leaves with a mycelium plug or spraying a conidium suspension on healthy potted plants) reproduced field symptoms, and the pathogen was re-isolated, thereby fulfilling Koch’s postulates. In vitro fungicide assays showed that carbendazim, tebuconazole, and prochloraz were highly effective against the pathogen, providing preliminary information for chemical management. This is the first documentation of C. siamense causing leaf anthracnose on C. officinalis and provides a basis for developing targeted control strategies to mitigate disease impacts and preserve yield and quality. Full article

The plant microbiome plays important roles in plant growth and resistance, but its assembly and affecting factors have not been fully studied for most of the agricultural plants. In this study, the endophytic mycobiota of the leaves and roots and the rhizosphere soils of five pummelo varieties were profiled based on the amplicon sequencing of the fungal internal transcribed spacer (ITS). The fungal richness and diversity were significantly different among the compartments, but not among the pummelo varieties. The composition and structure of the endophytic mycobiota of the compartments were significantly different across all five pummelo varieties. These suggest that the variety effect is weaker than the compartment effect, but still significant in shaping the pummelo mycobiota. Specifically, the dominant leaf endophytic fungal taxa (e.g., Fusarium and Zasmidium), and the root selection of fungal genera from the rhizosphere soils, were significantly different among the varieties. And also, the variety effect is more significant in shaping the leaf endophytic mycobiota than those of the roots. Finally, the pummelo varieties also showed some consistent alterations on the endophytic mycobiota, such as the root enrichment of Exophiala species. Our study indicates that the endophytic mycobiota of pummelos is significantly and interactively affected by plant variety and compartment effects, and suggests some fungi of interest for further tests. Full article

Although best known as a major parasite of sheep and goats, the blood-feeding abomasal nematode Haemonchus contortus can also infect cattle and buffaloes under the mixed-grazing Mediterranean conditions prevalent in Greece. The objectives of this study were as follows: (i) to determine the prevalence of H. contortus infections in dairy and beef cattle and buffaloes in Greece through an abattoir survey, (ii) to evaluate potential host- and farm-related risk factors including age, sex, management system, cattle productive orientation, and the co-existence of cattle and buffaloes on the occurrence of haemonchosis, and (iii) to assess the likelihood of detecting homozygous benzimidazole (BZ)-resistant H. contortus in large ruminant populations in relation to these determinants. A total of 213 abomasa (115, 55, and 43 from dairy, beef cattle, and buffaloes, respectively) were examined. A structured questionnaire provided additional animal- and farm-level information. Haemonchus-like helminths were collected and molecularly identified at the species level by amplifying a 321 bp fragment of the internal transcribed spacer 2 region of nuclear DNA. An allele-specific multiplex PCR, targeting codon 200 of the β-tubulin gene, was applied to detect BZ-resistant alleles. The prevalence of H. contortus infection was 21.2% in cattle and 69.8% in buffaloes. In cattle, multivariable analysis revealed that mixed-species farming (i.e., farms where cattle were the primary species and buffaloes were kept in smaller numbers), productive orientation, and slaughter age were significant predictors of increased H. contortus infection. Controversially, none of these factors were significantly associated with infection in buffaloes. Finally, multivariable modelling suggested that resistance patterns varied by host species, being more prevalent in intensively managed, older cattle, yet less common among older buffaloes and in herds where both species coexisted. Full article

Phytomycobiomes refer to the fungal consortia that inhabit plant tissues and the rhizosphere. Their documented functions include nutrient mobilization, carbon retention, stress mitigation and pathogen suppression, although measurable effects often depend on plant and soil conditions. In this review, we examine the current evidence for their ecological relevance and assess the molecular approaches most commonly used to characterize them. Arbuscular Mycorrhizal (AM) fungi, endophytes and saprotrophic taxa indicate measurable gains in nutrient acquisition, disease resistance and soil aggregation, although long-term consistency is rarely evaluated. Each function appears to have an explicit mechanistic attribution, with direct links between fungal groups, enzymatic pathways and measurable ecosystem outcomes. Several sequencing-based techniques are available, yet none offer complete accuracy. Internal Transcribed Spacer (ITS) amplicon surveys provide rapid taxonomic coverage but suffer from primer bias; shotgun metagenomics offers functional insight but at significant financial cost; and quantitative polymerase chain reaction (qPCR) assays remain useful for targeted quantification, whereas long-read technologies show promise but still lack widespread adoption. The field faces a number of unresolved constraints, including limited knowledge of host range, inconsistent performance under fluctuating environmental conditions and the absence of a standardized bioinformatic pipeline. Despite these limitations, we regard phytomycobiomes as viable candidates for replacing or reducing synthetic inputs, provided their application is guided by context-specific evidence rather than broad generalization. Full article

Honeyberry plants (Lonicera caerulea L., family Caprifoliaceae), which produce small, highly nutritious berries, have recently been subject to an outbreak of root rot, resulting in a drastic decrease in fruit yield. In this study, we isolated the fungal community from the roots of diseased plants and analyzed the mechanisms of interaction between key fungi and honeyberry plants. Nine fungal morphotypes were identified via observation of cultures and internal transcribed spacer gene sequence analysis. Pathogenicity assays showed that infection with isolates from the genus Fusarium reproduced the typical root rot symptoms, and Fusarium foetens caused the most severe inhibition of root growth. Transcriptomic analysis of infected plants and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the biosynthesis of secondary metabolites was enriched in roots of honeyberry plants infected with F. foetens. This study elucidates that when honeyberry is affected by root rot disease, it produces secondary metabolites to defend against pathogenic invasion, providing a theoretical basis and molecular targets for the green management of this disease. Full article

This case report describes a male bottlenose dolphin (Tursiops truncatus) from a republic aquarium in Quanzhou City, Fujian Province, China, in 2024. The dolphin exhibited prolonged vomiting that did not improve despite extended antibiotic treatment, followed by progressive deterioration in physical condition until death. Antemortem biochemical analyses indicated hepatic dysfunction (ALT: 269.8 IU/L, AST: 1357.5 IU/L, LDH: 2913.3 IU/L) and renal impairment (TBIL: 55.84 μmol/L, BUN: 31.93 mmol/L, Cr: 200.2 μmol/L). Necropsy showed atrophy of coronary fat in the heart, hepatomegaly with extensive yellow discoloration, splenomegaly with congestion, diffuse dark-red discoloration of the lungs, renal atrophy, segmental dark-red discoloration of the intestines, and dark-red enlargement of intestinal lymph nodes. Histopathological examination revealed hepatic steatosis with necrosis, extensive pulmonary hemorrhage with foreign bodies in the trachea and alveoli, intestinal necrosis with visible fungus, and congestion and necrosis of intestinal lymph nodes with visible fungus present; the fungus hyphae were periodic acid–Schiff (PAS)-positive. Fungal PCR targeting the fungus internal transcribed spacer (ITS) region identified the intestine fungus as Cladosporium. Infection with Cladosporium is extremely rare, and this report highlights the potential risks of emerging infectious diseases in marine mammals. Full article

Surveys conducted in a nursery located in eastern Sicily, southern Italy, revealed the presence of plants of Vachellia nilotica (syn. Acacia arabica), V. farnesiana (syn. A. farnesiana) and Pithecellobium dulce showing symptoms of trunk and branch canker, shoot dieback and general decline. Laboratory fungal isolation from wood tissues showed high percentage of Diaporthe-like (60–62%) and Botryosphaeriaceae-like fungi (21–85%) constantly associated with the diseased samples. Subsequent molecular characterization of recovered isolates was based on sequencing of the complete internally transcribed spacer region (ITS), the translation elongation factor 1-alpha (tef1) and the beta-tubulin (tub2) regions, followed by multi-locus phylogenetic analyses. The isolates collected from symptomatic tissues were phylogenetically characterized as Diaporthe foeniculina and Neofusicoccum parvum. Pathogenicity tests were conducted on Acacia and P. dulce plants and results showed that both species were pathogenic, being able to induce necrotic lesions on the stem. To our knowledge this is the first report worldwide of D. foeniculina and N. parvum infecting A. arabica, A. farnesiana and P. dulce. Full article

Acer saccharum Marsh., valued for its ornamental, material, and edible uses, is an important temperate tree species in the Northern Hemisphere. A blight disease affecting branches of A. saccharum was first identified in 2023 in Shandong, China. The causal agent was identified as Botryosphaeria wangensis G.Q. Li & S.F. Chen based on cultural and morphometric characteristics. Phylogenetic analysis was performed by amplifying and sequencing the internal transcribed spacer (ITS) region of rDNA, the translation elongation factor 1α (tef1) partial gene, the β-tubulin (tub2) partial gene, and the second largest subunit of RNA polymerase II (rpb2), in combination with morphological data. Symptoms observed in the field were replicated in a pathogenicity test through inoculation of A. saccharum branches, thus satisfying Koch’s postulates. To our knowledge, this is the first report worldwide of B. wangensis infecting A. saccharum. Full article

Members of the genus Termitomyces frequently grow in association with termites. During the monsoon season of 2022 and 2023, a total number of 13 Termitomyces samples were collected from the Chiang Mai University campus, Thailand. The objective of this study was to identify the collected samples. Based on morphological and molecular investigations, six distinct species were identified from the collected specimens. Five species (T. griseobulbus, T. griseobrunneus, T. planiperforatorius, T. pseudoheimii, and T. salmonicolor) are described herein as new to science, while one species (T. acriumbonatus) represents a new record for Thailand. The multi-gene phylogenetic analysis of the large subunit (nrLSU) of nuclear ribosomal DNA, the small subunit of mitochondrial DNA (mtSSU), and the nuclear internal transcribed spacer (nrITS) sequences confirmed that all six species belong to the genus Termitomyces. Full morphological descriptions, colour photographs, illustrations, and comparisons with phylogenetically and morphologically related species are provided. Full article