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29 pages, 2673 KiB  
Review
Integrating Large Language Models into Digital Manufacturing: A Systematic Review and Research Agenda
by Chourouk Ouerghemmi and Myriam Ertz
Computers 2025, 14(8), 318; https://doi.org/10.3390/computers14080318 (registering DOI) - 7 Aug 2025
Abstract
Industries 4.0 and 5.0 are based on technological advances, notably large language models (LLMs), which are making a significant contribution to the transition to smart factories. Although considerable research has explored this phenomenon, the literature remains fragmented and lacks an integrative framework that [...] Read more.
Industries 4.0 and 5.0 are based on technological advances, notably large language models (LLMs), which are making a significant contribution to the transition to smart factories. Although considerable research has explored this phenomenon, the literature remains fragmented and lacks an integrative framework that highlights the multifaceted implications of using LLMs in the context of digital manufacturing. To address this limitation, we conducted a systematic literature review, analyzing 53 papers selected according to predefined inclusion and exclusion criteria. Our descriptive and thematic analyses, respectively, mapped new trends and identified emerging themes, classified into three axes: (1) manufacturing process optimization, (2) data structuring and innovation, and (3) human–machine interaction and ethical challenges. Our results revealed that LLMs can enhance operational performance and foster innovation while redistributing human roles. Our research offers an in-depth understanding of the implications of LLMs. Finally, we propose a future research agenda to guide future studies. Full article
(This article belongs to the Special Issue AI in Complex Engineering Systems)
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16 pages, 5519 KiB  
Article
The Performance of a Novel Automated Algorithm in Estimating Truckload Volume Based on LiDAR Data
by Mihai Daniel Niţă, Cătălin Cucu-Dumitrescu, Bogdan Candrea, Bogdan Grama, Iulian Iuga and Stelian Alexandru Borz
Forests 2025, 16(8), 1281; https://doi.org/10.3390/f16081281 - 5 Aug 2025
Abstract
Significant improvements in the forest-based industrial sector are expected due to increased digitalization; however, examples of practical implementations remain limited. This study explores the use of an automated algorithm to estimate truckload volumes based on 3D point cloud data acquired using two different [...] Read more.
Significant improvements in the forest-based industrial sector are expected due to increased digitalization; however, examples of practical implementations remain limited. This study explores the use of an automated algorithm to estimate truckload volumes based on 3D point cloud data acquired using two different LiDAR scanning platforms. This research compares the performance of a professional mobile laser scanning (MLS GeoSLAM) platform and a smartphone-based iPhone LiDAR system. A total of 48 truckloads were measured using a combination of manual, factory-based, and digital approaches. Accuracy was evaluated using standard error metrics, including the mean absolute error (MAE) and root mean square error (RMSE), with manual or factory references used as benchmarks. The results showed a strong correlation and no significant differences between the algorithmic and manual measurements when using the MLS platform (MAE = 2.06 m3; RMSE = 2.46 m3). For the iPhone platform, the results showed higher deviations and significant overestimation compared to the factory reference (MAE = 3.29 m3; RMSE = 3.60 m3). Despite these differences, the iPhone platform offers real-time acquisition and low-cost deployment. These findings highlight the trade-offs between precision and operational efficiency and support the adoption of automated measurement tools in timber supply chains. Full article
(This article belongs to the Section Forest Operations and Engineering)
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13 pages, 1841 KiB  
Article
Valorizing Biomass Waste: Hydrothermal Carbonization and Chemical Activation for Activated Carbon Production
by Fidel Vallejo, Diana Yánez, Luis Díaz-Robles, Marcelo Oyaneder, Serguei Alejandro-Martín, Rasa Zalakeviciute and Tamara Romero
Biomass 2025, 5(3), 45; https://doi.org/10.3390/biomass5030045 - 5 Aug 2025
Viewed by 20
Abstract
This study optimizes the production of activated carbons from hydrothermally carbonized (HTC) biomass using potassium hydroxide (KOH) and phosphoric acid (H3PO4) as activating agents. A 23 factorial experimental design evaluated the effects of agent-to-precursor ratio, dry impregnation time, [...] Read more.
This study optimizes the production of activated carbons from hydrothermally carbonized (HTC) biomass using potassium hydroxide (KOH) and phosphoric acid (H3PO4) as activating agents. A 23 factorial experimental design evaluated the effects of agent-to-precursor ratio, dry impregnation time, and activation duration on mass yield and iodine adsorption capacity. KOH-activated carbons achieved superior iodine numbers (up to 1289 mg/g) but lower mass yields (18–35%), reflecting enhanced porosity at the cost of material loss. Conversely, H3PO4 activation yielded higher mass retention (up to 54.86%) with moderate iodine numbers (up to 1117.3 mg/g), balancing porosity and yield. HTC pretreatment at 190 °C reduced the ash content, thereby enhancing the stability of hydrochar. These findings highlight the trade-offs between adsorption performance and process efficiency, with KOH suited for high-porosity applications (e.g., water purification) and H3PO4 for industrial scalability. The study advances biomass waste valorization, aligning with circular economy principles and offering sustainable solutions for environmental and industrial applications, such as water purification and energy storage. Full article
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34 pages, 1227 KiB  
Review
Beyond Cutting: CRISPR-Driven Synthetic Biology Toolkit for Next-Generation Microalgal Metabolic Engineering
by Limin Yang and Qian Lu
Int. J. Mol. Sci. 2025, 26(15), 7470; https://doi.org/10.3390/ijms26157470 - 2 Aug 2025
Viewed by 345
Abstract
Microalgae, with their unparalleled capabilities for sunlight-driven growth, CO2 fixation, and synthesis of diverse high-value compounds, represent sustainable cell factories for a circular bioeconomy. However, industrial deployment has been hindered by biological constraints and the inadequacy of conventional genetic tools. The advent [...] Read more.
Microalgae, with their unparalleled capabilities for sunlight-driven growth, CO2 fixation, and synthesis of diverse high-value compounds, represent sustainable cell factories for a circular bioeconomy. However, industrial deployment has been hindered by biological constraints and the inadequacy of conventional genetic tools. The advent of CRISPR-Cas systems initially provided precise gene editing via targeted DNA cleavage. This review argues that the true transformative potential lies in moving decisively beyond cutting to harness CRISPR as a versatile synthetic biology “Swiss Army Knife”. We synthesize the rapid evolution of CRISPR-derived tools—including transcriptional modulators (CRISPRa/i), epigenome editors, base/prime editors, multiplexed systems, and biosensor-integrated logic gates—and their revolutionary applications in microalgal engineering. These tools enable tunable gene expression, stable epigenetic reprogramming, DSB-free nucleotide-level precision editing, coordinated rewiring of complex metabolic networks, and dynamic, autonomous control in response to environmental cues. We critically evaluate their deployment to enhance photosynthesis, boost lipid/biofuel production, engineer high-value compound pathways (carotenoids, PUFAs, proteins), improve stress resilience, and optimize carbon utilization. Persistent challenges—species-specific tool optimization, delivery efficiency, genetic stability, scalability, and biosafety—are analyzed, alongside emerging solutions and future directions integrating AI, automation, and multi-omics. The strategic integration of this CRISPR toolkit unlocks the potential to engineer robust, high-productivity microalgal cell factories, finally realizing their promise as sustainable platforms for next-generation biomanufacturing. Full article
(This article belongs to the Special Issue Developing Methods and Molecular Basis in Plant Biotechnology)
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14 pages, 1863 KiB  
Article
Advancements in Hole Quality for AISI 1045 Steel Using Helical Milling
by Pedro Mendes Silva, António José da Fonseca Festas, Robson Bruno Dutra Pereira and João Paulo Davim
J. Manuf. Mater. Process. 2025, 9(8), 256; https://doi.org/10.3390/jmmp9080256 - 31 Jul 2025
Viewed by 187
Abstract
Helical milling presents a promising alternative to conventional drilling for hole production, offering superior surface quality and improved production efficiency. While this technique has been extensively applied in the aerospace industry, its potential for machining common engineering materials, such as AISI 1045 steel, [...] Read more.
Helical milling presents a promising alternative to conventional drilling for hole production, offering superior surface quality and improved production efficiency. While this technique has been extensively applied in the aerospace industry, its potential for machining common engineering materials, such as AISI 1045 steel, remains underexplored in the literature. This study addresses this gap by systematically evaluating the influence of key process parameters—cutting speed (Vc), axial depth of cut (ap), and tool diameter (Dt)—on hole quality attributes, including surface roughness, burr formation, and nominal diameter accuracy. A full factorial experimental design (23) was employed, coupled with analysis of variance (ANOVA), to quantify the effects and interactions of these parameters. The results reveal that, with a higher Vc, it is possible to reduce surface roughness (Ra) by 30% to 40%, while an increased ap leads to a 50% increase in Ra. Additionally, Dt emerged as the most critical factor for nominal diameter accuracy, reducing geometrical errors by 1% with a larger Dt. Burr formation was predominantly observed at the lower end of the hole, highlighting challenges specific to this technique. These findings provide valuable insights into optimizing helical milling for low-carbon steels, offering a foundation for broader industrial adoption and further research. Full article
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30 pages, 924 KiB  
Review
Wood-Based Panels and Volatile Organic Compounds (VOCs): An Overview on Production, Emission Sources and Analysis
by Fátima Daniela Gonçalves, Luísa Hora Carvalho, José António Rodrigues and Rui Miguel Ramos
Molecules 2025, 30(15), 3195; https://doi.org/10.3390/molecules30153195 - 30 Jul 2025
Viewed by 347
Abstract
The emission and presence of volatile organic compounds (VOCs) in the indoor air of houses and factories has been a growing topic of debate in the industry and related research fields. Given the extended times people in modern society spend indoors, monitoring VOCs [...] Read more.
The emission and presence of volatile organic compounds (VOCs) in the indoor air of houses and factories has been a growing topic of debate in the industry and related research fields. Given the extended times people in modern society spend indoors, monitoring VOCs is crucial due to the associated potential health hazards, with formaldehyde being particularly noteworthy. Wood and wood-based panels (WBPs) (the latter constituting a significant segment of the wood-transforming industry, being widely used in furniture, construction, and other applications) are known sources for the emission of VOCs to indoor air. In the case of the WBPs, the emission of VOCs depends on the type and species of wood, together with industrial processing and addition of additives. This review integrates perspectives on the production processes associated with WBPs, together with the evolving global regulations, and thoroughly examines VOC sources associated with WBPs, health risks from exposure, and current analytical methods utilized for VOC detection. It comprises an overview of the WBP industry, providing relevant definitions, descriptions of manufacturing processes and adhesive use, analysis of legal constraints, and explanations of VOC source identification and describing analysis techniques utilized for VOCs in WBPs. Full article
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16 pages, 5655 KiB  
Article
A Multi-Branch Deep Learning Framework with Frequency–Channel Attention for Liquid-State Recognition
by Minghao Wu, Jiajun Zhou, Shuaiyu Yang, Hao Wang, Xiaomin Wang, Haigang Gong and Ming Liu
Electronics 2025, 14(15), 3028; https://doi.org/10.3390/electronics14153028 - 29 Jul 2025
Viewed by 200
Abstract
In the industrial production of polytetrafluoroethylene (PTFE), accurately recognizing the liquid state within the coagulation vessel is critical to achieving better product quality and higher production efficiency. However, the complex and subtle changes in the coagulation process pose significant challenges for traditional sensing [...] Read more.
In the industrial production of polytetrafluoroethylene (PTFE), accurately recognizing the liquid state within the coagulation vessel is critical to achieving better product quality and higher production efficiency. However, the complex and subtle changes in the coagulation process pose significant challenges for traditional sensing methods, calling for more reliable visual approaches that can handle varying scales and dynamic state changes. This study proposes a multi-branch deep learning framework for classifying the liquid state of PTFE emulsions based on high-resolution images captured in real-world factory conditions. The framework incorporates multi-scale feature extraction through a three-branch network and introduces a frequency–channel attention module to enhance feature discrimination. To address optimization challenges across branches, contrastive learning is employed for deep supervision, encouraging consistent and informative feature learning. The experimental results show that the proposed method significantly improves classification accuracy, achieving a mean F1-score of 94.3% across key production states. This work demonstrates the potential of deep learning-based visual classification methods for improving automation and reliability in industrial production. Full article
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36 pages, 1411 KiB  
Review
A Critical Analysis and Roadmap for the Development of Industry 4-Oriented Facilities for Education, Training, and Research in Academia
by Ziyue Jin, Romeo M. Marian and Javaan S. Chahl
Appl. Syst. Innov. 2025, 8(4), 106; https://doi.org/10.3390/asi8040106 - 29 Jul 2025
Viewed by 537
Abstract
The development of Industry 4-oriented facilities in academia for training and research purposes is playing a significant role in pushing forward the Fourth Industrial Revolution. This study can serve academic staff who are intending to build their Industry 4 facilities, to better understand [...] Read more.
The development of Industry 4-oriented facilities in academia for training and research purposes is playing a significant role in pushing forward the Fourth Industrial Revolution. This study can serve academic staff who are intending to build their Industry 4 facilities, to better understand the key features, constraints, and opportunities. This paper presents a systematic literature review of 145 peer-reviewed studies published between 2011 and 2023, which are identified across Scopus, SpringerLink, and Web of Science. As a result, we emphasise the significance of developing Industry 4 learning facilities in academia and outline the main design principles of the Industry 4 ecosystems. We also investigate and discuss the key Industry 4-related technologies that have been extensively used and represented in the reviewed literature, and summarise the challenges and roadblocks that current participants are facing. From these insights, we identify research gaps, outline technology mapping and maturity level, and propose a strategic roadmap for future implementation of Industry 4 facilities. The results of the research are expected to support current and future participants in increasing their awareness of the significance of the development, clarifying the research scope and objectives, and preparing them to deal with inherent complexity and skills issues. Full article
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27 pages, 3211 KiB  
Article
Hybrid Deep Learning-Reinforcement Learning for Adaptive Human-Robot Task Allocation in Industry 5.0
by Claudio Urrea
Systems 2025, 13(8), 631; https://doi.org/10.3390/systems13080631 - 26 Jul 2025
Viewed by 530
Abstract
Human-Robot Collaboration (HRC) is pivotal for flexible, worker-centric manufacturing in Industry 5.0, yet dynamic task allocation remains difficult because operator states—fatigue and skill—fluctuate abruptly. I address this gap with a hybrid framework that couples real-time perception and double-estimating reinforcement learning. A Convolutional Neural [...] Read more.
Human-Robot Collaboration (HRC) is pivotal for flexible, worker-centric manufacturing in Industry 5.0, yet dynamic task allocation remains difficult because operator states—fatigue and skill—fluctuate abruptly. I address this gap with a hybrid framework that couples real-time perception and double-estimating reinforcement learning. A Convolutional Neural Network (CNN) classifies nine fatigue–skill combinations from synthetic physiological cues (heart-rate, blink rate, posture, wrist acceleration); its outputs feed a Double Deep Q-Network (DDQN) whose state vector also includes task-queue and robot-status features. The DDQN optimises a multi-objective reward balancing throughput, workload and safety and executes at 10 Hz within a closed-loop pipeline implemented in MATLAB R2025a and RoboDK v5.9. Benchmarking on a 1000-episode HRC dataset (2500 allocations·episode−1) shows the hybrid CNN+DDQN controller raises throughput to 60.48 ± 0.08 tasks·min−1 (+21% vs. rule-based, +12% vs. SARSA, +8% vs. Dueling DQN, +5% vs. PPO), trims operator fatigue by 7% and sustains 99.9% collision-free operation (one-way ANOVA, p < 0.05; post-hoc power 1 − β = 0.87). Visual analyses confirm responsive task reallocation as fatigue rises or skill varies. The approach outperforms strong baselines (PPO, A3C, Dueling DQN) by mitigating Q-value over-estimation through double learning, providing robust policies under stochastic human states and offering a reproducible blueprint for multi-robot, Industry 5.0 factories. Future work will validate the controller on a physical Doosan H2017 cell and incorporate fairness constraints to avoid workload bias across multiple operators. Full article
(This article belongs to the Section Systems Engineering)
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26 pages, 5395 KiB  
Article
Understanding Urban Growth and Shrinkage: A Study of the Modern Manufacturing City of Dongguan, China
by Tingting Chen, Zhoutong Wu and Wei Lang
Land 2025, 14(8), 1507; https://doi.org/10.3390/land14081507 - 22 Jul 2025
Viewed by 511
Abstract
Since the early 21st century, urban shrinkage has become a significant global phenomenon. Dongguan, in Guangdong Province, China, is known as a “world factory”. It experienced notable urban shrinkage following the 2008 financial crisis. However, the city demonstrated remarkable recovery and ongoing development [...] Read more.
Since the early 21st century, urban shrinkage has become a significant global phenomenon. Dongguan, in Guangdong Province, China, is known as a “world factory”. It experienced notable urban shrinkage following the 2008 financial crisis. However, the city demonstrated remarkable recovery and ongoing development in subsequent years. On that basis, this study focuses on the following three points: (1) identifying the spatiotemporal factors contributing to the growth and shrinkage of manufacturing cities, taking Dongguan as an example; (2) explaining the influencing factors of the growth and shrinkage of Dongguan City during three critical periods, 2008–2014 (post-crisis), 2015–2019 (as machinery replaced human work), and 2020–2023 (the COVID-19 pandemic and recovery); and (3) selecting representative towns and streets for on-site observation and investigation, analyzing the measures they have taken to cope with growth and shrinkage during different periods. The key findings include the following: (1) The spatial dynamics of growth and shrinkage in Dongguan show significant temporal patterns, with traditional manufacturing areas shrinking from 2008 to 2014, central urban areas recovering from 2015 to 2019, and renewed shrinkage from 2020 to 2023. However, some regions maintained stability through strategic innovations. (2) Various factors, particularly industrial upgrading and technological innovation, drove the urban dynamics, enhancing economic resilience. (3) The case study of Houjie Town revealed successful adaptive mechanisms supported by policy while facing challenges like labor mismatches and inadequate R&D investment. This research offers insights for improving urban resilience and promoting sustainable development in Dongguan. Full article
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8 pages, 934 KiB  
Proceeding Paper
Optimizing Order Scheduling in Morocco’s Garment Industry for Fast Fashion: A K-Means Clustering-Driven Approach
by Abdelfattah Mouloud, Yasmine El Belghiti, Samir Tetouani, Omar Cherkaoui and Aziz Soulhi
Eng. Proc. 2025, 97(1), 50; https://doi.org/10.3390/engproc2025097050 - 21 Jul 2025
Viewed by 189
Abstract
The Moroccan garment industry faces challenges in scheduling small order batches, often hindered by traditional product family-based methods that increase downtime by 15–20%. This study proposes a clustering-based scheduling approach, grouping garments by technological times rather than product families to reduce changeovers and [...] Read more.
The Moroccan garment industry faces challenges in scheduling small order batches, often hindered by traditional product family-based methods that increase downtime by 15–20%. This study proposes a clustering-based scheduling approach, grouping garments by technological times rather than product families to reduce changeovers and downtime by 30–35%. A case study in a Moroccan factory with 50–100-unit batches showed a 20% lead time reduction and a 15% productivity boost. Using methods like K-Means, the approach enhances planning flexibility and resource use. This methodology offers a scalable solution for optimizing production and maintaining competitiveness in fast fashion markets. Full article
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19 pages, 2785 KiB  
Article
Implementing an AI-Based Digital Twin Analysis System for Real-Time Decision Support in a Custom-Made Sportswear SME
by Tõnis Raamets, Kristo Karjust, Jüri Majak and Aigar Hermaste
Appl. Sci. 2025, 15(14), 7952; https://doi.org/10.3390/app15147952 - 17 Jul 2025
Viewed by 325
Abstract
Small and medium-sized enterprises (SMEs) in the manufacturing sector often struggle to make effective use of production data due to fragmented systems and limited digital infrastructure. This paper presents a case study of implementing an AI-enhanced digital twin in a custom sportswear manufacturing [...] Read more.
Small and medium-sized enterprises (SMEs) in the manufacturing sector often struggle to make effective use of production data due to fragmented systems and limited digital infrastructure. This paper presents a case study of implementing an AI-enhanced digital twin in a custom sportswear manufacturing SME developed under the AI and Robotics Estonia (AIRE) initiative. The solution integrates real-time production data collection using the Digital Manufacturing Support Application (DIMUSA); data processing and control; clustering-based data analysis; and virtual simulation for evaluating improvement scenarios. The framework was applied in a live production environment to analyze workstation-level performance, identify recurring bottlenecks, and provide interpretable visual insights for decision-makers. K-means clustering and DBSCAN were used to group operational states and detect process anomalies, while simulation was employed to model production flow and assess potential interventions. The results demonstrate how even a lightweight AI-driven system can support human-centered decision-making, improve process transparency, and serve as a scalable foundation for Industry 5.0-aligned digital transformation in SMEs. Full article
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27 pages, 2260 KiB  
Article
Machine Learning for Industrial Optimization and Predictive Control: A Patent-Based Perspective with a Focus on Taiwan’s High-Tech Manufacturing
by Chien-Chih Wang and Chun-Hua Chien
Processes 2025, 13(7), 2256; https://doi.org/10.3390/pr13072256 - 15 Jul 2025
Viewed by 768
Abstract
The global trend toward Industry 4.0 has intensified the demand for intelligent, adaptive, and energy-efficient manufacturing systems. Machine learning (ML) has emerged as a crucial enabler of this transformation, particularly in high-mix, high-precision environments. This review examines the integration of machine learning techniques, [...] Read more.
The global trend toward Industry 4.0 has intensified the demand for intelligent, adaptive, and energy-efficient manufacturing systems. Machine learning (ML) has emerged as a crucial enabler of this transformation, particularly in high-mix, high-precision environments. This review examines the integration of machine learning techniques, such as convolutional neural networks (CNNs), reinforcement learning (RL), and federated learning (FL), within Taiwan’s advanced manufacturing sectors, including semiconductor fabrication, smart assembly, and industrial energy optimization. The present study draws on patent data and industrial case studies from leading firms, such as TSMC, Foxconn, and Delta Electronics, to trace the evolution from classical optimization to hybrid, data-driven frameworks. A critical analysis of key challenges is provided, including data heterogeneity, limited model interpretability, and integration with legacy systems. A comprehensive framework is proposed to address these issues, incorporating data-centric learning, explainable artificial intelligence (XAI), and cyber–physical architectures. These components align with industrial standards, including the Reference Architecture Model Industrie 4.0 (RAMI 4.0) and the Industrial Internet Reference Architecture (IIRA). The paper concludes by outlining prospective research directions, with a focus on cross-factory learning, causal inference, and scalable industrial AI deployment. This work provides an in-depth examination of the potential of machine learning to transform manufacturing into a more transparent, resilient, and responsive ecosystem. Additionally, this review highlights Taiwan’s distinctive position in the global high-tech manufacturing landscape and provides an in-depth analysis of patent trends from 2015 to 2025. Notably, this study adopts a patent-centered perspective to capture practical innovation trends and technological maturity specific to Taiwan’s globally competitive high-tech sector. Full article
(This article belongs to the Special Issue Machine Learning for Industrial Optimization and Predictive Control)
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24 pages, 1605 KiB  
Article
Quantum-Secure Coherent Optical Networking for Advanced Infrastructures in Industry 4.0
by Ofir Joseph and Itzhak Aviv
Information 2025, 16(7), 609; https://doi.org/10.3390/info16070609 - 15 Jul 2025
Viewed by 459
Abstract
Modern industrial ecosystems, particularly those embracing Industry 4.0, increasingly depend on coherent optical networks operating at 400 Gbps and beyond. These high-capacity infrastructures, coupled with advanced digital signal processing and phase-sensitive detection, enable real-time data exchange for automated manufacturing, robotics, and interconnected factory [...] Read more.
Modern industrial ecosystems, particularly those embracing Industry 4.0, increasingly depend on coherent optical networks operating at 400 Gbps and beyond. These high-capacity infrastructures, coupled with advanced digital signal processing and phase-sensitive detection, enable real-time data exchange for automated manufacturing, robotics, and interconnected factory systems. However, they introduce multilayer security challenges—ranging from hardware synchronization gaps to protocol overhead manipulation. Moreover, the rise of large-scale quantum computing intensifies these threats by potentially breaking classical key exchange protocols and enabling the future decryption of stored ciphertext. In this paper, we present a systematic vulnerability analysis of coherent optical networks that use OTU4 framing, Media Access Control Security (MACsec), and 400G ZR+ transceivers. Guided by established risk assessment methodologies, we uncover critical weaknesses affecting management plane interfaces (e.g., MDIO and I2C) and overhead fields (e.g., Trail Trace Identifier, Bit Interleaved Parity). To mitigate these risks while preserving the robust data throughput and low-latency demands of industrial automation, we propose a post-quantum security framework that merges spectral phase masking with multi-homodyne coherent detection, strengthened by quantum key distribution for key management. This layered approach maintains backward compatibility with existing infrastructure and ensures forward secrecy against quantum-enabled adversaries. The evaluation results show a substantial reduction in exposure to timing-based exploits, overhead field abuses, and cryptographic compromise. By integrating quantum-safe measures at the optical layer, our solution provides a future-proof roadmap for network operators, hardware vendors, and Industry 4.0 stakeholders tasked with safeguarding next-generation manufacturing and engineering processes. Full article
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21 pages, 4047 KiB  
Article
Valorization of Spent Coffee Grounds as a Substrate for Fungal Laccase Production and Biosorbents for Textile Dye Decolorization
by Eduardo da Silva França, Adriana Ferreira de Souza, Dayana Montero Rodríguez, Nazareth Zimiani de Paula, Anna Gabrielly Duarte Neves, Kethylen Barbara Barbosa Cardoso, Galba Maria de Campos-Takaki, Marcos Antonio Barbosa de Lima and Ana Lucia Figueiredo Porto
Fermentation 2025, 11(7), 396; https://doi.org/10.3390/fermentation11070396 - 10 Jul 2025
Viewed by 484
Abstract
Spent coffee grounds (SCG) are a widely available agro-industrial residue rich in carbon and phenolic compounds, presenting significant potential for biotechnological valorization. This study evaluated the use of SCG as a suitable substrate for fungal laccase production and the application of the resulting [...] Read more.
Spent coffee grounds (SCG) are a widely available agro-industrial residue rich in carbon and phenolic compounds, presenting significant potential for biotechnological valorization. This study evaluated the use of SCG as a suitable substrate for fungal laccase production and the application of the resulting fermented biomass (RFB), a mixture of fermented SCG and fungal biomass as a biosorbent for textile dye removal. Two fungal strains, namely Lentinus crinitus UCP 1206 and Trametes sp. UCP 1244, were evaluated in both submerged (SmF) and solid-state fermentation (SSF) using SCG. L. crinitus showed superior performance in SSF, reaching 14.62 U/g of laccase activity. Factorial design revealed that a lower SCG amount (5 g) and higher moisture (80%) and temperature (30 °C ± 0.2) favored enzyme production. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) analyses confirmed significant structural degradation of SCG after fermentation, especially in SSF. Furthermore, SCG and RFB were chemically activated and evaluated as biosorbents. The activated carbon from SCG (ACSCG) and RFB (ACRFB) exhibited high removal efficiencies for Remazol dyes, comparable to commercial activated carbon. These findings highlight the potential of SCG as a low-cost, sustainable resource for enzyme production and wastewater treatment, contributing to circular bioeconomy strategies. Full article
(This article belongs to the Special Issue Application and Research of Solid State Fermentation, 2nd Edition)
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