Search Results (659)

Temperature fluctuations caused by climate change and global warming pose a threat to fish. The burbot (lota lota) population is particularly sensitive to increased water temperature, but the systematic impacts of high-temperature exposure on their liver and intestinal health remain unclear. In January of 2025, we collected wild adult burbot individuals from the Ussuri River (water temperature: about 2 °C), China. The burbot were exposed to 2 °C, 7 °C, 12 °C, 17 °C, and 22 °C environments for 96 h; then, the liver and intestinal contents were subsequently collected for histopathology observation, immunohistochemistry, biochemical index assessment, and transcriptome/16S rDNA sequencing analysis. There was obvious liver damage including hepatocyte necrosis, fat vacuoles, and cellular peripheral nuclei. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities were elevated and subsequently decreased. Additionally, the malondialdehyde (MDA) level significantly increased with increasing temperature. These results indicate that 7 °C (heat stress temperature), 12 °C (tipping point for normal physiological metabolism status), 17 °C (tipping point for individual deaths), and 22 °C (thermal limit) are critical temperatures in terms of the physiological response of burbot during their breeding period. In the hepatic transcriptome profiling, 6538 differentially expressed genes (DEGs) were identified, while KEGG enrichment analysis showed that high-temperature stress could affect normal liver function by regulating energy metabolism, immune, and apoptosis-related pathways. Microbiomics also revealed that acute heat stress could change the intestinal microbe community structure. Additionally, correlation analysis suggested potential regulatory relationships between intestinal microbe taxa and immune/apoptosis-related DEGs in the liver. This study revealed the potential impact of environmental water temperature changes in cold habitats in winter on the physiological adaptability of burbot during the breeding period and provides new insights for the ecological protection of burbot in the context of global climate change and habitat warming. Full article

High-temperature stress has become an important factor that has restricted the aquaculture industry. Huso dauricus is a high-economic-value fish that has faced the threat of thermal stress. Based on this point, our investigation aimed to explore the detailed mechanism of the negative impacts of heat stress on the liver metabolism functions in Huso dauricus. In this study, we set one control group (19 °C) and four high-temperature treatment groups (22 °C, 25 °C, 28 °C, 31 °C) with 40 fish in each group for continuous 53-day heat exposure. Histological analysis, biochemical detection, and transcriptome technology were used to explore the effects of heat stress on the liver structure and functions of juvenile Huso dauricus. It suggested heat-stress-induced obvious liver injury and reactive oxygen species accumulation in Huso dauricus with a time/temperature-dependent manner. Serum total protein, transaminase, and alkaline phosphatase activities showed significant changes under heat stress (p < 0.05). In addition, 6433 differentially expressed genes (DEGs) were identified based on the RNA-seq project. Gene Ontology enrichment analysis showed that various DEGs could be mapped to the lipid-metabolism-related terms. KEGG enrichment and immunohistochemistry analysis showed that ferroptosis and FoxO signaling pathways were significantly enriched (p < 0.05). These results demonstrated that thermal stress induced oxidative stress damage in the liver of juvenile Huso dauricus, which triggered lipid metabolism disorder and hepatocyte ferroptosis to disrupt normal liver functions. In conclusion, chronic thermal stress can cause antioxidant capacity imbalance in the liver of Huso dauricus to mediate the ferroptosis process, which would finally disturb the lipid metabolism homeostasis. In further research, it will be necessary to verify the detailed cellular signaling pathways that are involved in the heat-stress-induced liver function disorder response based on the in vitro experiment, while the multi-organ crosswalk mode under the thermal stress status is also essential for understanding the comprehensive mechanism of heat-stress-mediated negative effects on fish species. Full article

The shape, size, and interfacial transition zone (ITZ) of aggregates significantly impact the nonlinear mechanical behavior of concrete. This study investigates concrete’s mechanical response and damage mechanisms by developing a three-dimensional, three-phase mesoscale model comprising coarse aggregates, mortar, and ITZ to explore the compressive performance of concrete. A method for simulating the random distribution of aggregates based on three-dimensional grid partitioning is proposed, where the value of each grid point represents the maximum aggregate radius that can be accommodated if the point serves as the aggregate center. Aggregates are generated by randomly selecting grid points that meet specific conditions, avoiding overlapping distributions and significantly improving computational efficiency as the generation progresses. This model effectively enhances the precision and efficiency of aggregate distribution and provides a reliable tool for studying the random distribution characteristics of aggregates in concrete. Additionally, an efficient discrete element model (DEM) was established based on this mesoscale model to simulate the compressive behavior of concrete, including failure modes and stress–strain curves. The effects of aggregate shape and maximum aggregate size on the uniaxial compressive failure behavior of concrete specimens were investigated. Aggregate shape has a particular influence on the compressive strength of concrete, and the compressive strength decreases with an increase in maximum aggregate size. Combined with existing experimental results, the proposed mesoscale model demonstrates high reliability in analyzing the compressive performance of concrete, providing valuable insights for further research on the mechanical properties of concrete. Full article

In recent years, the number of fake news stories has significantly increased in the world of media, especially with the widespread use of social media. It has impacted several industries, including tourism. From a tourism point of view, the spread of fake news can contribute to the reduction of the popularity of a destination. It may influence travel decisions by discouraging tourists from visiting certain places and thus damage the reputation of the destination, contributing to economic loss. After a literature review on the communication aspect of fake news and a general introduction of fake news in the tourism and hospitality industry, we conducted a systematic literature review (SLR), a research methodology to collect, identify, and analyse available research studies through a systematic procedure. The current SLR is based on the Scopus, Web of Science, and Google Scholar databases of existing literature on the topic of fake news in the tourism and hospitality industry. The study identifies, lists, and examines existing papers and conference proceedings from a vast array of disciplines, in order to give a well-rounded view on the issue of fake news in the tourism and hospitality industry. After selecting a total of 54 previous studies from more than 20 thousand results for the keywords ‘fake news’ and ‘tourism,’ we have analysed 39 papers in total. The SLR aimed to highlight existing gaps in the literature and areas that may require further exploration in future primary research. We have found that there is relatively limited academic literature available on the subject of fake news affecting tourism destinations, compared to studies focused on hospitality services. Full article

Blasting is one of the most widely used and cost-effective techniques for rock excavation and fragmentation in open-pit mining, particularly for large-scale operations. However, repeated or poorly controlled blasting can generate excessive ground vibrations that threaten slope stability by causing structural damage, fracturing of the rock mass, and potential failure. Evaluating the effects of blast-induced vibrations is essential to ensure safe and sustainable mining operations. This study investigates the impact of blasting-induced vibrations on slope stability at the Saindak Copper-Gold Open-Pit Mine in Pakistan. A comprehensive dataset was compiled, including field-monitored ground vibration measurements—specifically peak particle velocity (PPV) and key blast design parameters such as spacing (S), burden (B), stemming length (SL), maximum charge per delay (MCPD), and distance from the blast point (D). Geomechanical properties of slope-forming rock units were validated through laboratory testing. Slope stability was analyzed using pseudo-static limit equilibrium methods (LEMs) based on the Mohr–Coulomb failure criterion, employing four approaches: Fellenius, Janbu, Bishop, and Spencer. Pearson and Spearman correlation analyses quantified the influence of blasting parameters on slope behavior, and sensitivity analysis determined the cumulative distribution of slope failure and dynamic response under increasing seismic loads. FoS values were calculated for both east and west pit slopes under static and dynamic conditions. Among all methods, Spencer consistently yielded the highest FoS values. Under static conditions, FoS was 1.502 for the east slope and 1.254 for the west. Under dynamic loading, FoS declined to 1.308 and 1.102, reductions of 12.9% and 11.3%, respectively, as calculated using the Spencer method. The east slope exhibited greater stability due to its gentler angle. Correlation analysis revealed that burden had a significant negative impact (r = −0.81) on stability. Sensitivity analysis showed that stability deteriorates notably when PPV exceeds 10.9 mm/s. Although daily blasting did not critically compromise stability, the west slope showed greater vulnerability, underscoring the need for stricter control of blasting energy to mitigate vibration-induced instability and promote long-term operational sustainability. Full article

When a high-speed rotating projectile faces high impact loads, the sensitive parts of the control system can get damaged, resulting in operational failure. It is crucial to develop a unique buffer structure that offers impact resistance and has a small contact area. An annularly distributed multi-sphere point contact structure was designed and fabricated on a magnesium alloy substrate based on the Hertz contact theory. The accuracy of the finite element numerical model, constructed using Abaqus/Explicit, was verified through hydraulic impact tests. The impact mechanical properties of the structure were studied by analyzing the influence of the number, diameter, and cavity radius of hemispheres using an experimentally verified finite element model. The axial and radial deformations of the structure were compared and analyzed. The research findings indicate that the deformation and impact resistance of the structure can be greatly influenced by increasing the number of hemispheres, enlarging the hemisphere diameter, and incorporating internal cavities. Specifically, with 6 hemispheres, each with a diameter of Φ 6 mm and a cavity radius of R1.5 mm, the axial and radial deformations are only 1.03 mm and 3.02 mm, respectively. The contact area of a single hemisphere is 7.16 mm². The study offers new perspectives on choosing buffer structures in high-impact environments. Full article

The time-dependent creep behavior of coal is essential for assessing long-term structural stability and operational safety in deep coal mining. Therefore, this work develops a full-stage creep constitutive model. By integrating fractional calculus theory with statistical damage mechanics, a nonlinear fractional-order (FO) damage creep model is constructed through serial connection of elastic, viscous, viscoelastic, and viscoelastic–plastic components. Based on this model, both one-dimensional and three-dimensional (3D) fractional creep damage constitutive equations are acquired. Model parameters are identified using experimental data from deep coal samples in the mining area. The result curves of the improved model coincide with experimental data points, accurately describing the deceleration creep stage (DCS), steady-state creep stage (SCS), and accelerated creep stage (ACS). Furthermore, a sensitivity analysis elucidates the impact of model parameters on coal creep behavior, thereby confirming the model’s robustness and applicability. Consequently, the proposed model offers a solid theoretical basis for evaluating the sustained stability of deep coal mining and has great application potential in deep underground engineering. Full article

Background/Objectives: Silicon dioxide nanoparticles (SiO₂NPs) do not exist in isolation in the environment but can interact with other substances, thus influencing their toxic effects on aquatic organisms. We assessed the combined impact of SiO₂NPs and N-acetylcysteine (NAC), an antioxidant with the potential to counteract nanoparticle-induced oxidative stress (OS). Methods: Behavioral assessments, including the social interaction test and color preference test, were performed to evaluate neurobehavioral changes. OS biomarkers, including malondialdehyde (MDA) levels for lipid peroxidation and the activity of key antioxidant enzymes such as glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD), were assessed to evaluate the extent of cellular damage. Results: The results indicate that prolonged exposure to SiO₂NPs induces significant behavioral disruptions, including reduced exploratory behavior and increased anxiety-like responses. Furthermore, biochemical analysis revealed increased OS, suggesting nanoparticle-induced cellular toxicity. NAC co-treatment partially reversed these effects, particularly improving locomotor outcomes and antioxidant response, but was less effective on social behavior. Conclusions: These findings highlight the ecological and health risks posed by SiO₂NPs and point toward the need for further toxicological studies on their long-term biological effects. Full article

Point-of-care (POC) diagnostic technologies have become essential for the real-time monitoring and management of chronic wounds, where maintaining a moist environment and controlling pH levels are critical for effective healing. In this study, a flexible pH sensor based on a graphene/molybdenum disulfide (graphene/MoS₂) composite interdigitated electrode (IDE) structure was fabricated using pulsed laser ablation. The pH sensor, with an active area of 30 mm × 30 mm, exhibited good adhesion to the polyethylene terephthalate (PET) substrate and maintained structural integrity under repeated bending cycles. Precise ablation was achieved under optimized conditions of 4.35 J/cm² laser fluence, a repetition rate of 300 kHz, and a scanning speed of 500 mm/s, enabling the formation of defect-free IDE arrays without substrate damage. The influence of laser processing parameters on the surface morphology, electrical conductivity, and wettability of the composite thin films was systematically characterized. The fabricated pH sensor exhibited high sensitivity (~4.7% change in current per pH unit) across the pH 2–10 range, rapid response within ~5.2 s, and excellent mechanical stability under 100 bending cycles with negligible performance degradation. Moreover, the sensor retained > 95% of its stable sensitivity after 7 days of ambient storage. Furthermore, the pH response behavior was evaluated for electrode structures with different pitches, demonstrating that structural design parameters critically impact sensing performance. These results offer valuable insights into the scalable fabrication of flexible, wearable pH sensors, with promising applications in wound monitoring and personalized healthcare systems. Full article

Three-dimensional (3D) reproduction of artworks has advanced significantly, offering valuable insights for conservation by documenting the objects’ conservative state at both macroscopic and microscopic scales. This paper presents the 3D survey of an earthquake-damaged panel painting, whose wooden support suffered severe deformation during a seismic event, posing unique restoration challenges. Our work focuses on quantifying how shape variations in the support—induced during restoration—affect the surface morphology of the pictorial layers. To this end, we conducted measurements before and after support consolidation using two complementary 3D techniques: structured-light projection to generate 3D models of the painting, tracking global shape changes in the panel, and laser-scanning microprofilometry to produce high-resolution models of localized areas, capturing surface morphology, superficial cracks, and pictorial detachments. By processing and cross-comparing 3D point cloud data from both techniques, we quantified shape variations and evaluated their impact on the pictorial layers. This approach demonstrates the utility of multi-scale 3D documentation in guiding complex restoration interventions. Full article

Aero-engine casings with excellent impact resistance are a practical requirement for ensuring the safe operation of aero-engines. In this paper, we report on numerical simulations of broken rotating blades impacting aluminum honeycomb sandwich casings under different temperatures and optimization of structural parameters. Firstly, an impact test system with adjustable temperature was established. Restricted by the temperature range of the strain gauge, ballistic impact tests were carried out at 25 °C, 100 °C, and 200 °C. Secondly, a finite element (FE) model including a pointed bullet and an aluminum honeycomb sandwich plate was built using LS-DYNA. The corresponding simulations of the strain–time curve and damage conditions showed good agreement with the test results. Then, the containment capability of the aluminum honeycomb sandwich aero-engine casing at different temperatures was analyzed based on the kinetic energy loss of the blade, the internal energy increment of the casing, and the containment state of the blade. Finally, with the design objectives of minimizing the casing mass and maximizing the blade kinetic energy loss, the structural parameters of the casing were optimized using the multi-objective genetic algorithm (MOGA). Full article

Background/Objectives: Antibiotic resistance presents an urgent public health threat. By developing a streamlined and effective method for studying bacteriophage induction, this research marks a step further in understanding how antibiotic-resistant genes might spread across different environments. This knowledge is essential for creating strategies to reduce the spread of antimicrobial resistance (AMR), particularly from a One Health perspective. In this study, we develop and validate a Green Fluorescent Protein (GFP)-based method as a proxy for bacteriophage induction. This method screens compounds for their potential to promote bacteriophage induction. Methods: This study utilized a recA-GFP construct in Escherichia coli to measure fluorescence as an indicator of SOS response activation. The experiments involved treating E. coli cultures with varying concentrations of the DNA-damaging chemical mitomycin C and measuring fluorescence over time. Additionally, droplet digital PCR (ddPCR) quantified bacteriophage induction in a lambda phage-carrying E. coli strain, allowing for correlation analysis between the two methods. Results: The recA-driven SOS response depended on both dose and time, with increasing concentrations of mitomycin C leading to higher fluorescence. ddPCR analysis confirmed that mitomycin C induced prophage activation, with gene ratios increasing at higher drug concentrations over time. A strong Spearman correlation (>0.7) was noted between fluorescence and ddPCR results at elevated concentrations and relevant time points, indicating the validity of the GFP-based model as a proxy for bacteriophage induction. Conclusions: The findings demonstrate a strong association between the two methods of measuring phage induction, suggesting that the GFP-based E. coli model is a reliable, cost-effective, and efficient tool for studying phage induction and its potential role in AMR spread. This method could facilitate the screening of environmental samples and specific drugs to evaluate their impact on bacteriophage induction, which opens the door for applications such as screening for antibiotic resistance dissemination. Full article

In high-pressure thermal power systems, corrosion-induced wall thinning in steam pipelines poses a significant threat to operational safety and efficiency. This study investigates the effects of gas–liquid two-phase flow on corrosion-induced wall thinning in pipe bends of high-pressure heaters in power plants, with particular emphasis on the mechanisms of void fraction and inner wall surface roughness. Research reveals that an increased void fraction significantly enhances flow turbulence and centrifugal effects, resulting in elevated pressure and Discrete Phase Model (DPM) concentration at the bend, thereby intensifying erosion phenomena. Simultaneously, the turbulence generated by bubble collapse at the bend promotes the accumulation and detachment of corrosion products, maintaining a cyclic process of erosion and corrosion that accelerates wall thinning. Furthermore, the increased surface roughness of the inner bend wall exacerbates the corrosion process. The rough surface alters local flow characteristics, leading to changes in pressure distribution and DPM concentration accumulation points, subsequently accelerating corrosion progression. Energy-Dispersive Spectroscopy (EDS) and Scanning Electron Microscopy (SEM) analyses reveal changes in the chemical composition and microstructural characteristics of corrosion products. The results indicate that the porous structure of oxide films fails to effectively protect against corrosive media, while bubble impact forces damage the oxide films, exposing fresh metal surfaces and further accelerating the corrosion process. Comprehensive analysis demonstrates that the interaction between void fraction and surface roughness significantly intensifies wall thinning, particularly under conditions of high void fraction and high roughness, where pressure and DPM concentration at the bend may reach extreme values, further increasing corrosion risk. Therefore, optimization of void fraction and surface roughness, along with the application of corrosion-resistant materials and surface treatment technologies, should be considered in pipeline design and operation to mitigate corrosion risks. Full article

Background: Salmonella infections impose a substantial global health burden, with an estimated 95.1 million cases occurring annually. Pregnant women exhibit a heightened vulnerability due to pregnancy-specific immune adaptations and dietary habits that increase their risk of Salmonella exposure, facilitating possible damage to the placental barrier. Despite this significant burden, Salmonella-associated placental pathology remains poorly understood, particularly its impact on foetal development through microstructural alterations. Aim: This study utilised stereology to assess histomorphological and functional alterations in term placentae of Salmonella Typhi-exposed placentae, compared to unexposed controls. Methods: A hospital-based case-control study was conducted in Ghana. Of 237 screened women, 62 placentae were selected for analysis, comprising 31 Salmonella-exposed cases (IgG/IgM-positive in placental and cord blood) and 31 gestational age-matched controls (IgG/IgM-negative). Placental tissues were processed for histology and stereology. Neonatal birthweights were also compared. Results: Stereological assessment revealed significantly higher mean volume densities of syncytial knots in the study group (0.4755 ± 0.04) compared to the controls (0.3342 ± 0.04, p = 0.0219). Syncytial denudation was increased in the study group (0.8113 ± 0.09) relative to the controls (0.1975 ± 0.08, p < 0.0001). Foetal capillary volume density was also significantly elevated in the study group (5.1010 ± 0.32) compared to the controls (3.562 ± 0.47, p < 0.0001). In contrast, intervillous space volume was significantly reduced in the study group (9.5810 ± 0.05) compared to the controls (11.593 ± 0.26, p = 0.0053). Neonates of exposed mothers showed a non-significant reduction in birthweight. Conclusion: Salmonella Typhi exposure in pregnancy induces subtle, yet significant alterations in placental architecture, compromising villous integrity and vascular organisation. Although birthweight may appear unaffected, the observed changes point to reduced placental efficiency and merit further research into their developmental consequences and long-term effects on babies. Full article

Background: This study investigates the effects of a tocotrienol-enriched drink on oxidative damage and genomic stability in older adults over a 6-month period. Methods: A total of 67 participants (27 males and 40 females, mean age 60.45 ± 5.75 years) were enrolled in this double-blinded, two-arm, parallel randomized controlled trial. Baseline, mid-point, and end-point assessments were conducted to monitor various health parameters. Significant Group × Time interaction effects were observed for several key outcomes. Results: Group A demonstrated significantly better improvements in QOL-Psychological (p = 0.014, Partial Eta Squared = 0.153), suggesting a beneficial impact of tocotrienol supplementation on mental well-being. Additionally, Group A showed more favorable trends in TNF-α (p = 0.04), T-SOD (p = 0.04), catalase (p = 0.02), and telomerase (p = 0.02), suggesting potential antioxidant and genomic stability improvements over time. Conclusions: In a nutshell, tocotrienol supplementation may exert beneficial effects on psychological well-being, oxidative stress modulation, and genomic stability in aging populations. Full article