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12 pages, 1362 KiB  
Article
Physiological Response to Foliar Application of Antitranspirant on Avocado Trees (Persea americana) in a Mediterranean Environment
by Giulia Modica, Fabio Arcidiacono, Stefano La Malfa, Alessandra Gentile and Alberto Continella
Horticulturae 2025, 11(8), 928; https://doi.org/10.3390/horticulturae11080928 (registering DOI) - 6 Aug 2025
Abstract
Background: The implementation of advanced agronomical strategies, including the use of antitranspirant, in order to mitigate the negative effects of environmental stress, particularly heat stress on plants, has become a focal area of research in the Mediterranean basin. This region is characterized by [...] Read more.
Background: The implementation of advanced agronomical strategies, including the use of antitranspirant, in order to mitigate the negative effects of environmental stress, particularly heat stress on plants, has become a focal area of research in the Mediterranean basin. This region is characterized by hot and dry summer that affects plant physiology. Methods: The experiment was carried out in Sicily (South Italy) on 12-year-old avocado cv. Hass grafted onto Walter Hole rootstock. Two subplots each of forty homogenous trees were selected and treated (1) with calcium carbonate (DECCO Shield®) and (2) with water (control) at the following phenological phases: 711, 712 and 715 BBCH. The climatic parameters were recorded throughout the year. Physiological measurements (leaf transpiration, net photosynthesis, stomatal conductance, leaf water potential) were measured at 105, 131 and 168 days after full bloom. Fruit growth was monitored, and physico-chemical analyses were carried out at harvest. Results: The antitranspirant increased photosynthesis and stomatal conductance and reduced leaf transpiration (−26.1%). Fruit growth rate increased during summer, although no morphological and qualitative difference was observed at harvest. PCA highlighted the positive effect of the calcium carbonate on overall plant physiology. Conclusions: Antitranspirant foliar application reduced heat stress effects by improving physiological responses of avocado trees. Full article
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14 pages, 842 KiB  
Article
Immunoenhancing Effects of Jeju Agastache rugosa Extracts on NK Cell Activity and Lymphocyte Proliferation in Cyclophosphamide-Induced Immunosuppression
by Sung Jin Kim, Seong-Il Kang, Nari Lee, Jung Min Oh, Hiyoung Kim, Mi-Gi Lee, Ji Hoon Song and Myoung-Sook Shin
Pharmaceuticals 2025, 18(8), 1129; https://doi.org/10.3390/ph18081129 - 28 Jul 2025
Viewed by 286
Abstract
Background/objectives: This study evaluated the immunoenhancing effects of Agastache rugosa extract in a cyclophosphamide-induced immunosuppressed mouse model. Methods: Jeju A. rugosa was processed via hot water extraction and 20% ethanol extraction. For immunosuppression induction, 7-week-old male BALB/c mice received intraperitoneal CPA injections (150 [...] Read more.
Background/objectives: This study evaluated the immunoenhancing effects of Agastache rugosa extract in a cyclophosphamide-induced immunosuppressed mouse model. Methods: Jeju A. rugosa was processed via hot water extraction and 20% ethanol extraction. For immunosuppression induction, 7-week-old male BALB/c mice received intraperitoneal CPA injections (150 mg/kg, day −3; 110 mg/kg, day −1), followed by oral administration of hot water extract (ARE-W) and ethanol extract (ARE-E) at 100 and 300 mg/kg for 14 days. Oral administration of ARE-W and ARE-E was started on day 0, immediately following the final CPA injection on day −1. Immune function was assessed through body weight changes, spleen weight, NK cell activity, IFN-γ production, and splenic lymphocyte proliferation. Results: Results demonstrated that CPA treatment induced comprehensive immune dysfunction, while A. rugosa extracts significantly ameliorated these immunosuppressive conditions. Notably, ARE-W (300 mg/kg) significantly enhanced NK cell cytotoxicity against tumor cells and IFN-γ production compared to the CPA group, and effectively restored spleen weight and lymphocyte proliferation. ARE-E also exhibited dose-dependent immune function recovery; however, ARE-W showed superior efficacy across most immune parameters. Conclusions: These findings suggest that A. rugosa extract, particularly ARE-W, effectively restores immune function in immunosuppressed conditions, indicating potential application as a natural functional material for ameliorating immunosuppression caused by cancer treatment or immune diseases. Full article
(This article belongs to the Special Issue Natural Products in Health Promotion and Disease Prevention 2025)
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24 pages, 1412 KiB  
Article
Arthrospira platensis var. toliarensis: A Local Sustainable Microalga for Food System Resilience
by Antonio Fidinirina Telesphore, Andreea Veronica Botezatu, Daniela Ionela Istrati, Bianca Furdui, Rodica Mihaela Dinica and Valérie Lalao Andriamanamisata Razafindratovo
Foods 2025, 14(15), 2634; https://doi.org/10.3390/foods14152634 - 27 Jul 2025
Viewed by 349
Abstract
The intensifying global demand for sustainable and nutrient-dense food sources necessitates the exploration of underutilized local resources. Arthrospira platensis var. toliarensis, a cyanobacterium endemic to Madagascar, was evaluated for its nutritional, functional, and environmental potential under small-scale, low-input outdoor cultivation. The study [...] Read more.
The intensifying global demand for sustainable and nutrient-dense food sources necessitates the exploration of underutilized local resources. Arthrospira platensis var. toliarensis, a cyanobacterium endemic to Madagascar, was evaluated for its nutritional, functional, and environmental potential under small-scale, low-input outdoor cultivation. The study assessed growth kinetics, physicochemical parameters, and composition during two contrasting seasons. Biomass increased 7.5-fold in 10 days, reaching a productivity of 7.8 ± 0.58 g/m2/day and a protein yield of 4.68 ± 0.35 g/m2/day. The hot-season harvest showed significantly higher protein content (65.1% vs. 44.6%), enriched in essential amino acids. On a dry matter basis, mineral profiling revealed high levels of sodium (2140 ± 35.4 mg/100 g), potassium (1530 ± 21.8 mg/100 g), calcium (968 ± 15.1 mg/100 g), phosphorus (815 ± 13.2 mg/100 g), magnesium (389.28 ± 6.4 mg/100 g), and iron (235 ± 9.1 mg/100 g), underscoring its value as a micronutrient-rich supplement. The hydroethanolic extract had the highest polyphenol content (4.67 g GAE/100 g of dry extract), while the hexanic extract exhibited the strongest antioxidant capacity (IC50 = 101.03 ± 1.37 µg/mL), indicating fat-soluble antioxidants. Aflatoxin levels (B1, B2, G1, and G2) remained below EU safety thresholds. Compared to soy and beef, this strain showed superior protein productivity and water-use efficiency. These findings confirm A. platensis var. toliarensis as a promising, ecologically sound alternative for improving food and nutrition security, and its local production can offer substantial benefits to smallholder livelihoods. Full article
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17 pages, 5495 KiB  
Article
Application of Empirical Mode Decomposition to Land Surface Temperature Projection Under a Changing Climate
by Che-Wei Chang and Wen-Cheng Huang
Water 2025, 17(15), 2204; https://doi.org/10.3390/w17152204 - 23 Jul 2025
Viewed by 282
Abstract
This study takes the daily temperature series of Taipei City as an example and proposes a data projection method based on Empirical Mode Decomposition (EMD), which effectively resolves the challenge of modeling non-stationary sequences. According to the daily mean temperature records from 1971 [...] Read more.
This study takes the daily temperature series of Taipei City as an example and proposes a data projection method based on Empirical Mode Decomposition (EMD), which effectively resolves the challenge of modeling non-stationary sequences. According to the daily mean temperature records from 1971 to 2023, Taipei has experienced an average warming rate of 0.02 °C per year. After applying EMD, the data were decomposed into 12 intrinsic mode functions (IMFs) and one residual trend. Among them, IMF5, with a period of 352 days (approximately one year), contributes 78% of the total energy, representing the dominant climatic cycle component. In this study, daily temperatures were categorized into five thermal levels: Cold (<12 °C), Cool (12–18 °C), Moderate (18–27 °C), Warm (27–32 °C), and Hot (>32 °C). In addition, using a 5-year moving process based on the annual EMD results, the IMFs and residuals were recombined to generate 390,625 synthetic sequences per year. Results show that the monthly mean temperatures of each year’s simulations closely match the observations, capturing the non-stationary characteristics of temperature variations. The overall classification accuracy of simulated versus observed daily temperature categories ranges from 60% to 71%, with an average of 65.1%. In summary, the EMD combined with the 5-year moving process developed in this study demonstrates a helpful data projection approach with effective reconstruction of periodic structures and stable simulation accuracy. It offers practical value for reconstructing urban climate variability, conducting risk assessments, and analyzing long-term warming trends. Moreover, it serves as a vital tool for modeling non-stationary climate data and supporting future projections. Full article
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16 pages, 2566 KiB  
Article
Human Responses to Different Built Hyperthermal Environments After Short-Term Heat Acclimation
by Shuai Zhang, Qingqin Wang, Haizhu Zhou, Tianyang Wang and Guanguan Jia
Buildings 2025, 15(14), 2581; https://doi.org/10.3390/buildings15142581 - 21 Jul 2025
Viewed by 254
Abstract
Hyperthermal environments are encountered in many situations, and significant heat stress can exacerbate the fatigue perception of individuals and potentially threaten their safety. Heat acclimation (HA) interventions have many benefits in preventing the risk of incidents. However, whether HA interventions in specific environments [...] Read more.
Hyperthermal environments are encountered in many situations, and significant heat stress can exacerbate the fatigue perception of individuals and potentially threaten their safety. Heat acclimation (HA) interventions have many benefits in preventing the risk of incidents. However, whether HA interventions in specific environments can cope with other different hyperthermal environments remains uncertain. In this study, forty-three young male participants were heat-acclimated over 10 days of training on a motorized treadmill in a fixed hyperthermal environment, and they were tested in different hyperthermal environments. Physiological indices (rectal temperature (Tr), heart rate (HR), skin temperature (Tsk), and total sweat loss (Msl)) and subjective perception (rating of perceived exertion (RPE) and thermal sensation votes (TSVs)) were measured during both the heat stress test (HST) sessions and HA training sessions. The results show that HR and Tsk significantly differed between pre- and post-heat acclimation (p < 0.05 for all) following the acclimation program. However, after heat acclimation training, the reduction in Tr (ΔTr) was more notable in lower-ET* environments, and Msl showed distinct changes in different ET* environments. The RPE and TSV decreased after HA interventions, although the difference was not significant. The results indicate that HA can effectively reduce the peak of physiological parameters. However, when subjected to stronger heat stress, the improvement effects of heat acclimation on human responses will be affected. In addition, HA can alleviate physiological thermal strain, thereby reducing the adverse effects on mobility, but it has no effect on the supervisor’s ability to perceive the environment. This study suggests that additional HA training can reduce the risk of activities in high-temperature environments but exhibits different effects under different environmental conditions, indicating that hot acclimation suits have selective effects on the environment. This study provides recommendations for additional HA training before high-temperature activities. Full article
(This article belongs to the Special Issue Low-Carbon Urban Areas and Neighbourhoods)
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20 pages, 6510 KiB  
Article
Research on the Operating Performance of a Combined Heat and Power System Integrated with Solar PV/T and Air-Source Heat Pump in Residential Buildings
by Haoran Ning, Fu Liang, Huaxin Wu, Zeguo Qiu, Zhipeng Fan and Bingxin Xu
Buildings 2025, 15(14), 2564; https://doi.org/10.3390/buildings15142564 - 20 Jul 2025
Viewed by 365
Abstract
Global building energy consumption is significantly increasing. Utilizing renewable energy sources may be an effective approach to achieving low-carbon and energy-efficient buildings. A combined system incorporating solar photovoltaic–thermal (PV/T) components with an air-source heat pump (ASHP) was studied for simultaneous heating and power [...] Read more.
Global building energy consumption is significantly increasing. Utilizing renewable energy sources may be an effective approach to achieving low-carbon and energy-efficient buildings. A combined system incorporating solar photovoltaic–thermal (PV/T) components with an air-source heat pump (ASHP) was studied for simultaneous heating and power generation in a real residential building. The back panel of the PV/T component featured a novel polygonal Freon circulation channel design. A prototype of the combined heating and power supply system was constructed and tested in Fuzhou City, China. The results indicate that the average coefficient of performance (COP) of the system is 4.66 when the ASHP operates independently. When the PV/T component is integrated with the ASHP, the average COP increases to 5.37. On sunny days, the daily average thermal output of 32 PV/T components reaches 24 kW, while the daily average electricity generation is 64 kW·h. On cloudy days, the average daily power generation is 15.6 kW·h; however, the residual power stored in the battery from the previous day could be utilized to ensure the energy demand in the system. Compared to conventional photovoltaic (PV) systems, the overall energy utilization efficiency improves from 5.68% to 17.76%. The hot water temperature stored in the tank can reach 46.8 °C, satisfying typical household hot water requirements. In comparison to standard PV modules, the system achieves an average cooling efficiency of 45.02%. The variation rate of the system’s thermal loss coefficient is relatively low at 5.07%. The optimal water tank capacity for the system is determined to be 450 L. This system demonstrates significant potential for providing efficient combined heat and power supply for buildings, offering considerable economic and environmental benefits, thereby serving as a reference for the future development of low-carbon and energy-saving building technologies. Full article
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19 pages, 920 KiB  
Article
Natural Alternatives for Pain Relief: A Study on Morus alba, Angelica archangelica, Valeriana officinalis, and Passiflora incarnata
by Felicia Suciu, Oana Cristina Șeremet, Emil Ștefănescu, Ciprian Pușcașu, Cristina Isabel Viorica Ghiță, Cerasela Elena Gîrd, Robert Viorel Ancuceanu and Simona Negreș
J. Mind Med. Sci. 2025, 12(2), 39; https://doi.org/10.3390/jmms12020039 - 19 Jul 2025
Viewed by 285
Abstract
Background: Chronic pain poses a major global health burden, often inadequately managed by conventional analgesics due to limited efficacy and side effects. In this context, plant-based therapies offer a promising alternative. This study aimed to evaluate the antioxidant and analgesic potential of four [...] Read more.
Background: Chronic pain poses a major global health burden, often inadequately managed by conventional analgesics due to limited efficacy and side effects. In this context, plant-based therapies offer a promising alternative. This study aimed to evaluate the antioxidant and analgesic potential of four medicinal plants traditionally used for pain relief: Morus alba, Angelica archangelica, Valeriana officinalis, and Passiflora incarnata. Methods: Phytochemical analyses quantified total phenolic acid, flavonoid, and polyphenolic acid contents in the extracts. Antioxidant activity was assessed using the ABTS radical scavenging assay. Analgesic effects were evaluated in vivo using the hot-plate and tail-flick tests in mice treated for 14 days with plant extracts or paracetamol. Results: Morus alba showed the highest polyphenolic content and strongest antioxidant activity (IC50 = 0.0695 mg/mL). In analgesic tests, Angelica archangelica demonstrated the most significant effect in the hot-plate test (72.2% increase in latency), while Valeriana officinalis had the highest efficacy in the tail-flick test (41.81%), exceeding paracetamol’s performance in that model. Conclusions: While antioxidant activity correlated with polyphenol content, analgesic effects appeared to involve additional mechanisms. These findings support the potential of Angelica archangelica and Valeriana officinalis as effective natural alternatives for pain relief. Full article
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13 pages, 5701 KiB  
Article
High-Fat/High-Sugar Diet and High-Temperature/High-Humidity Exposure Aggravates Ulcerative Colitis in an Experimental Mouse Model
by Pengyan Li, Guibing Meng, Ang Li, Liang Chen, Xinchi Feng and Feng Qiu
Curr. Issues Mol. Biol. 2025, 47(7), 562; https://doi.org/10.3390/cimb47070562 - 18 Jul 2025
Viewed by 375
Abstract
Ulcerative colitis (UC) is a subtype of inflammatory bowel disease (IBD) that has been associated with overconsumption of calories and lipids, compared to the healthy population, and summer temperatures have been reported to be closely related to the prevalence of UC. To evaluate [...] Read more.
Ulcerative colitis (UC) is a subtype of inflammatory bowel disease (IBD) that has been associated with overconsumption of calories and lipids, compared to the healthy population, and summer temperatures have been reported to be closely related to the prevalence of UC. To evaluate the effects of dietary and lifestyle factors on UC, a combination of 2.0% dextran sulfate sodium (DSS), a high-fat/high-sugar diet, and exposure to high temperature and humidity was used to construct mouse models of UC. Changes in body weight, disease activity index (DAI) scores, histopathological analysis, serum lipid levels, serum diamine oxidase (DAO), and D-Lactate (D-LA) levels, as well as the expression of inflammatory cytokines and tight junction proteins in colonic tissue, were all assessed to study the impacts of the high-fat/high-sugar diet and high-temperature/high-humidity exposure on the progression of UC. The symptoms observed in the UC mouse model induced by 2.0% DSS alone were similar to those seen in patients with UC, while the high-fat and high-sugar diet, along with humid and hot exposure, exacerbated DSS-induced UC in the mice. This included more severe histopathological damage to the colon tissue, increased expression of pro-inflammatory cytokines (IL-6, IL-17A, and IL-1β), and a more significantly compromised intestinal barrier, characterized by the destruction of ZO-1 and elevated levels of DAO and D-LA. Additionally, the high-fat/high-sugar diet and high-temperature/high-humidity exposure led to further disturbances in glucose and lipid metabolism in the mice, which were not observed in those treated with DSS alone. This study is the first to investigate the effects of a high-fat/high-sugar diet and high-temperature/high-humidity exposure on the progression of UC. Full article
(This article belongs to the Section Molecular Pharmacology)
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20 pages, 2422 KiB  
Article
Design and Performance of a Large-Diameter Earth–Air Heat Exchanger Used for Standalone Office-Room Cooling
by Rogério Duarte, António Moret Rodrigues, Fernando Pimentel and Maria da Glória Gomes
Appl. Sci. 2025, 15(14), 7938; https://doi.org/10.3390/app15147938 - 16 Jul 2025
Viewed by 233
Abstract
Earth–air heat exchangers (EAHXs) use the soil’s thermal capacity to dampen the amplitude of outdoor air temperature oscillations. This effect can be used in hot and dry climates for room cooling with no or very little need for resources other than those used [...] Read more.
Earth–air heat exchangers (EAHXs) use the soil’s thermal capacity to dampen the amplitude of outdoor air temperature oscillations. This effect can be used in hot and dry climates for room cooling with no or very little need for resources other than those used during the EAHX construction, an obvious advantage compared to the significant operational costs of refrigeration machines. Contrary to the streamlined process applied in conventional HVAC design (using refrigeration machines), EAHX design lacks straightforward and well-established rules; moreover, EAHXs struggle to achieve office room design cooling demands determined with conventional indoor thermal environment standards, hindering designers’ confidence and the wider adoption of EAHXs for standalone room cooling. This paper presents a graph-based method to assist in the design of a large-diameter EAHX. One year of post-occupancy monitoring data are used to evaluate this method and to investigate the performance of a large-diameter EAHX with up to 16,000 m3/h design airflow rate. Considering an adaptive standard for thermal comfort, peak EAHX cooling capacity of 28 kW (330 kWh/day, with just 50 kWh/day of fan electricity consumption) and office room load extraction of up to 22 kW (49 W/m2) provided evidence in support of standalone use of EAHX for room cooling. A fair fit between actual EAHX thermal performance and results obtained with the graph-based design method support the use of this method for large-diameter EAHX design. Full article
(This article belongs to the Special Issue Thermal Comfort and Energy Consumption in Buildings)
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21 pages, 2431 KiB  
Article
Up-Cycling Broccoli Stalks into Fresh-Cut Sticks: Postharvest Strategies for Quality and Shelf-Life Enhancement
by Nieves García-Lorca, José Ángel Salas-Millán and Encarna Aguayo
Foods 2025, 14(14), 2476; https://doi.org/10.3390/foods14142476 - 15 Jul 2025
Viewed by 279
Abstract
Broccoli stalks are considered an agro-industrial by-product that, in the context of fresh consumption, is undervalued, as only broccoli florets are typically marketed. This study evaluated the up-cycling of broccoli stalks into a value-added fresh-cut product through postharvest preservation strategies. Stalks were peeled, [...] Read more.
Broccoli stalks are considered an agro-industrial by-product that, in the context of fresh consumption, is undervalued, as only broccoli florets are typically marketed. This study evaluated the up-cycling of broccoli stalks into a value-added fresh-cut product through postharvest preservation strategies. Stalks were peeled, cut into sticks (8 × 8 mm × 50–100 mm), sanitised, packaged under modified atmosphere conditions, and stored at 5 °C. Treatments included (a) calcium ascorbate (CaAsc, 1% w/v), (b) trehalose (TREH, 5% w/v), (c) hot water treatment (HWT, 55 °C, 1 min), and several combinations of them. HWT alone was highly effective in reducing browning, a key factor for achieving an extended shelf-life, controlling microbial growth and respiration, and obtaining the highest sensory scores (appearance = 7.3 on day 11). However, it was less effective in preserving bioactive compounds. The HWT + CaAsc treatment proved to be the most effective at optimising quality and retaining health-promoting compounds. It increased vitamin C retention by 78%, antioxidant capacity by 68%, and total phenolic content by 65% compared to the control on day 11. This synergistic effect was attributed to the antioxidant action of ascorbic acid in CaAsc. TREH alone showed no preservative effect, inducing browning, elevated respiration, and microbial proliferation. Overall, combining mild thermal and antioxidant treatments offers a promising strategy to valorise broccoli stalks as fresh-cut snacks. An 11-day shelf-life at 5 °C was achieved, with increased content of health-promoting bioactive compounds, while supporting circular economy principles and contributing to food loss mitigation. Full article
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20 pages, 3918 KiB  
Article
Crop Evapotranspiration Dynamics in Morocco’s Climate-Vulnerable Saiss Plain
by Abdellah Oumou, Ali Essahlaoui, Mohammed El Hafyani, Abdennabi Alitane, Narjisse Essahlaoui, Abdelali Khrabcha, Ann Van Griensven, Anton Van Rompaey and Anne Gobin
Remote Sens. 2025, 17(14), 2412; https://doi.org/10.3390/rs17142412 - 12 Jul 2025
Viewed by 703
Abstract
The Saiss plain in northern Morocco covers an area of 2300 km2 and is one of the main agricultural contributors to the national economy. However, climate change and water scarcity reduce the region’s agricultural yields. Conventional methods of estimating evapotranspiration (ET) provide [...] Read more.
The Saiss plain in northern Morocco covers an area of 2300 km2 and is one of the main agricultural contributors to the national economy. However, climate change and water scarcity reduce the region’s agricultural yields. Conventional methods of estimating evapotranspiration (ET) provide localized results but cannot capture regional-scale variations. This study aims to estimate the spatiotemporal evolution of daily crop ET (olives, fruit trees, cereals, and vegetables) across the Saiss plain. The METRIC model was adapted for the region using Landsat 8 data and was calibrated and validated using in situ flux tower measurements. The methodology employed an energy balance approach to calculate ET as a residual of net radiation, soil heat flux, and sensible heat flux by using hot and cold pixels for calibration. METRIC-ET ranged from 0.1 to 11 mm/day, demonstrating strong agreement with reference ET (R2 = 0.76, RMSE = 1, MAE = 0.78) and outperforming MODIS-ET in accuracy and spatial resolution. Olives and fruit trees showed higher ET values compared to vegetables and cereals. The results indicated a significant impact of ET on water availability, with spatiotemporal patterns being influenced by vegetation cover, climate, and water resources. This study could support the development of adaptive agricultural strategies. Full article
(This article belongs to the Section Remote Sensing in Agriculture and Vegetation)
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17 pages, 3651 KiB  
Article
Moss Biochar Facilitates Root Colonization of Halotolerant Halomonas salifodinae for Promoting Plant Growth Under Saline–Alkali Stress
by Wenyue Wang, Yunlong Liu, Zirun Zhao, Rou Liu, Fang Wang, Zhuo Zhang and Qilin Yu
Soil Syst. 2025, 9(3), 73; https://doi.org/10.3390/soilsystems9030073 - 11 Jul 2025
Viewed by 205
Abstract
The utilization of the widely distributed saline–alkali lands by planting forage grasses is a hot topic. However, the promotion of plant growth remains a great challenge during the exploration of this stressful soil. While halotolerant bacteria are beneficial for plants against saline–alkali stress, [...] Read more.
The utilization of the widely distributed saline–alkali lands by planting forage grasses is a hot topic. However, the promotion of plant growth remains a great challenge during the exploration of this stressful soil. While halotolerant bacteria are beneficial for plants against saline–alkali stress, their stable colonization on plant roots should be further strengthened. In this study, we investigated the effect of moss biochar on the root colonization of the exogenous halotolerant Halomonas salifodinae isolated from saline lake sediments. During the incubation with the bacteria, the biochar strongly bound the bacterium and induced biofilm formation on the biochar surface. When the biochar and the bacterium were added into the culturing soil of the forage grass Medicago sativa, the biochar remarkably assisted the root binding and biofilm formation of this bacterium on the plant roots. Under the biochar–bacterium combined treatment, the numbers of total bacteria, halotolerant bacteria, and nitrogen-fixing bacteria increased from 105.5 CFU/g soil to 107.2 CFU/g soil, from 104.5 CFU/g soil to 106.1 CFU/g soil, and from 104.7 CFU/g soil to 106.3 CFU/g soil, respectively. After 30 days of culturing, the biochar and the bacterium in combination increased the plant height from 10.3 cm to 36 cm, and enhanced the accumulation of chlorophyll a, reducing sugars, soluble proteins, and superoxide dismutase in the leaves. Moreover, the combined treatment increased the activity of soil enzymes, including peroxidase, alkaline phosphatase, and urease. Meanwhile, the levels of various cations in the rhizosphere soil were reduced by the combined treatment, e.g., Na+, Cu2+, Fe2+, Mg2+, Mn2+, etc., indicating an improvement in the soil quality. This study developed the biochar–halotolerant bacterium joint strategy to improve the yield of forage grasses in saline–alkali soil. Full article
(This article belongs to the Special Issue Microbial Community Structure and Function in Soils)
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30 pages, 1884 KiB  
Article
Storage Behavior and Response to Low-Cost Postharvest Technologies of the Underutilized Purple Yampee (Dioscorea trifida L.f.)
by Sandra Viviana Medina-López, Jorge Andrés Jola Hernández, Maria Soledad Hernández-Gómez and Juan Pablo Fernández-Trujillo
Foods 2025, 14(14), 2436; https://doi.org/10.3390/foods14142436 - 10 Jul 2025
Viewed by 477
Abstract
Postharvest losses and limited physiological knowledge restrict the conservation and year-round availability of underutilized crops such as Dioscorea trifida. This study characterized the postharvest behavior of Colombian purple D. trifida tubers and evaluated low-cost, GRAS-status technologies to improve storage performance in smallholder [...] Read more.
Postharvest losses and limited physiological knowledge restrict the conservation and year-round availability of underutilized crops such as Dioscorea trifida. This study characterized the postharvest behavior of Colombian purple D. trifida tubers and evaluated low-cost, GRAS-status technologies to improve storage performance in smallholder production systems. Tubers were stored for 34 days at ambient conditions (20 °C, 90% RH) and compared with treatments including cold storage, calcium pretreatments combined with Aloe vera-based coatings, and short-duration hot water immersion. Over storage, total carbohydrates increased, while potassium remained at substantial levels until the final day. Weight loss and respiration declined steadily, and sprouting was absent, suggesting extended endodormancy in this genotype. Major deterioration causes observed upon reception included fragmentation, insect damage, and surface molds, highlighting the importance of improved sanitation and mechanical protection during harvest, early postharvest stages, and transportation. Edible coatings enhanced antioxidant activity and increased malic and succinic acid concentrations. Cold storage at 3 °C reduced weight loss more effectively than storage at 12 or 20 °C, although citric acid accumulation was greater at the latter temperature. Among all treatments, immersion at 55 °C for 5 min was the most promising, offering a scalable, low-input option to extend shelf life in neglected yam species. Full article
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26 pages, 4558 KiB  
Article
Enrichment of Rice Flour with Almond Bagasse Powder: The Impact on the Physicochemical and Functional Properties of Gluten-Free Bread
by Stevens Duarte, Janaina Sánchez-García, Joanna Harasym and Noelia Betoret
Foods 2025, 14(13), 2382; https://doi.org/10.3390/foods14132382 - 5 Jul 2025
Viewed by 437
Abstract
Almond bagasse, a by-product of almond milk production, is rich in fibre, protein, polyunsaturated fatty acids, and bioactive compounds. Its incorporation into food products provides a sustainable approach to reducing food waste while improving nutritional quality. This study explored the impact of enriching [...] Read more.
Almond bagasse, a by-product of almond milk production, is rich in fibre, protein, polyunsaturated fatty acids, and bioactive compounds. Its incorporation into food products provides a sustainable approach to reducing food waste while improving nutritional quality. This study explored the impact of enriching rice flour with almond bagasse powders—either hot air-dried (HAD60) or lyophilised (LYO)—at substitution levels of 5%, 10%, 15%, 20%, 25%, and 30% (w/w), to assess effects on gluten-free bread quality. The resulting flour blends were analysed for their physicochemical, techno-functional, rheological, and antioxidant properties. Gluten-free breads were then prepared using these blends and evaluated fresh and after seven days of refrigerated storage. The addition of almond bagasse powders reduced moisture and water absorption capacities, while also darkening the bread colour, particularly in HAD60, due to browning from thermal drying. The LYO powder led to softer bread by disrupting the starch structure more than HAD60. All breads hardened after storage due to starch retrogradation. The incorporation of almond bagasse powder reduced the pasting behaviour—particularly at substitution levels of ≥ 25%—as well as the viscoelastic moduli of the flour blends, due to fibre competing for water and thereby limiting starch gelatinisation. Antioxidant capacity was significantly enhanced in HAD60 breads, particularly in the crust and at higher substitution levels, due to Maillard reactions. Furthermore, antioxidant degradation over time was less pronounced in formulations with higher substitution levels, with HAD60 proving more stable than LYO. Overall, almond bagasse powder improves the antioxidant profile and shelf-life of gluten-free bread, highlighting its value as a functional and sustainable ingredient. Full article
(This article belongs to the Section Food Engineering and Technology)
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25 pages, 3047 KiB  
Article
Fate of Pyrrolizidine Alkaloids in Soil: Insights from Myosotis arvensis L. and Senecio vulgaris L.
by Ilva Nakurte, Gundars Skudriņš and Ieva Mežaka
Toxins 2025, 17(7), 335; https://doi.org/10.3390/toxins17070335 - 2 Jul 2025
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Abstract
Pyrrolizidine alkaloids are plant-derived toxins with environmental persistence and the potential to contaminate soil, water, and adjacent crops. This study investigated the leaching behavior and environmental fate of PAs from two PA-producing weeds—Myosotis arvensis L. (Boraginaceae) and Senecio vulgaris L. (Asteraceae)—in two [...] Read more.
Pyrrolizidine alkaloids are plant-derived toxins with environmental persistence and the potential to contaminate soil, water, and adjacent crops. This study investigated the leaching behavior and environmental fate of PAs from two PA-producing weeds—Myosotis arvensis L. (Boraginaceae) and Senecio vulgaris L. (Asteraceae)—in two Latvian agricultural soils: sandy loam and loam. Hot- and cold-water plant extracts were applied to soil columns (10 cm and 20 cm), and leachates were analyzed over a 14-day period using QuEChERS purification and LC-HRMS detection. Leaching varied by plant species, extract type, and soil. M. arvensis showed significantly higher cumulative leaching (77–84% for cold, 65–71% for hot extracts), attributed to the higher solubility of N-oxides. In contrast, S. vulgaris extracts leached minimally (<0.84% from sandy loam) and were undetectable in loam. The presence of cyclic diester PAs in S. vulgaris and the higher cation exchange capacity of loam favored retention or degradation. PANO-to-PA conversion occurred in both soils, indicating redox activity. The fate of PAs was influenced by structural type (diesters showing higher persistence), extraction method (hot extraction releasing more pyrrolizidine alkaloids), and soil properties such as pH, organic matter, and cation exchange capacity, which affected sorption and mobility. These findings underscore the significance of soil composition in controlling PA mobility and associated environmental risks. Future research should focus on long-term PA persistence across diverse soil types and investigate crop uptake potential and microbial degradation pathways under field conditions. Full article
(This article belongs to the Special Issue Toxic Plant-Derived Metabolites)
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