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19 pages, 1551 KiB  
Article
Genome-Wide Association Study Reveals Key Genetic Loci Controlling Oil Content in Soybean Seeds
by Xueyang Wang, Min Zhang, Fuxin Li, Xiulin Liu, Chunlei Zhang, Fengyi Zhang, Kezhen Zhao, Rongqiang Yuan, Sobhi F. Lamlom, Honglei Ren, Hongmei Qiu and Bixian Zhang
Agronomy 2025, 15(8), 1889; https://doi.org/10.3390/agronomy15081889 - 5 Aug 2025
Abstract
Seed oil represents a key trait in soybeans, which holds substantial economic significance, contributing to roughly 60% of global oilseed production. This research employed genome-wide association mapping to identify genetic loci associated with oil content in soybean seeds. A panel comprising 341 soybean [...] Read more.
Seed oil represents a key trait in soybeans, which holds substantial economic significance, contributing to roughly 60% of global oilseed production. This research employed genome-wide association mapping to identify genetic loci associated with oil content in soybean seeds. A panel comprising 341 soybean accessions, primarily sourced from Northeast China, was assessed for seed oil content at Heilongjiang Province in three replications over two growing seasons (2021 and 2023) and underwent genotyping via whole-genome resequencing, resulting in 1,048,576 high-quality SNP markers. Phenotypic analysis indicated notable variation in oil content, ranging from 11.00% to 21.77%, with an average increase of 1.73% to 2.28% across all growing regions between 2021 and 2023. A genome-wide association study (GWAS) analysis revealed 119 significant single-nucleotide polymorphism (SNP) loci associated with oil content, with a prominent cluster of 77 SNPs located on chromosome 8. Candidate gene analysis identified four key genes potentially implicated in oil content regulation, selected based on proximity to significant SNPs (≤10 kb) and functional annotation related to lipid metabolism and signal transduction. Notably, Glyma.08G123500, encoding a receptor-like kinase involved in signal transduction, contained multiple significant SNPs with PROVEAN scores ranging from deleterious (−1.633) to neutral (0.933), indicating complex functional impacts on protein function. Additional candidate genes include Glyma.08G110000 (hydroxycinnamoyl-CoA transferase), Glyma.08G117400 (PPR repeat protein), and Glyma.08G117600 (WD40 repeat protein), each showing distinct expression patterns and functional roles. Some SNP clusters were associated with increased oil content, while others correlated with decreased oil content, indicating complex genetic regulation of this trait. The findings provide molecular markers with potential for marker-assisted selection (MAS) in breeding programs aimed at increasing soybean oil content and enhancing our understanding of the genetic architecture governing this critical agricultural trait. Full article
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13 pages, 988 KiB  
Article
Assessing the Applicability of a Partial Alcohol Reduction Method to the Fine Wine Analytical Composition of Pinot Gris
by Diána Ágnes Nyitrainé Sárdy, Péter Bodor-Pesti and Szabina Steckl
Foods 2025, 14(15), 2738; https://doi.org/10.3390/foods14152738 - 5 Aug 2025
Abstract
Climate change has a significant negative impact on agriculture and food production. This trend requires technological development and the adaptation of new technologies in both the grapevine production and winemaking sectors. High temperatures and heat accumulation during the growing season result in faster [...] Read more.
Climate change has a significant negative impact on agriculture and food production. This trend requires technological development and the adaptation of new technologies in both the grapevine production and winemaking sectors. High temperatures and heat accumulation during the growing season result in faster ripening and a higher sugar content, leading to a higher alcohol content during fermentation. The negative consequences are an imbalanced wine character and consumer reluctance, as lower alcoholic beverages are now in high demand. Over the last decade, several methods have been developed to handle this impact and reduce the alcohol content of wines. In this study, we used the MASTERMIND® REMOVE membrane-based dealcoholization system to reduce the alcohol concentration in of Pinot gris wines from 12.02% v/v to 10.69% v/v and to investigate the effect on analytical parameters in three steps (0.5%, 1%, and 1.5% reductions) along the treatment. To evaluate the impact of the partial alcohol reduction and identify correlations between the wine chemical parameters, data were analyzed with ANOVA, PCA, multivariate linear regression and cluster analysis. The results showed that except for the extract, sugar content and proline content, the treatment had a significant effect on the chemical parameters. Both free and total SO2 levels were significantly reduced as well as volatile acid, glycerol and succinic acid levels. It must be highlighted that some parameters were not differing significantly between the untreated and the final wine, while the change was statistically verified in the intermediate steps of the partial alcohol reduction. This was the case for example for n-Propanol, i-Amylalcohol, Acetaldehyde, and Ethyl acetate. The multivariate linear regression model explained 18.84% of the total variance, indicating a modest but meaningful relationship between the alcohol content and the investigated analytical parameters. Our results showed that even if the applied instrument significantly modified some of the wine chemical parameters, those changes would not influence significantly the wine sensory attributes. Full article
(This article belongs to the Special Issue Winemaking: Innovative Technology and Sensory Analysis)
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30 pages, 1235 KiB  
Article
Assessing Rainfall and Temperature Trends in Central Ethiopia: Implications for Agricultural Resilience and Future Climate Projections
by Teshome Girma Tesema, Nigussie Dechassa Robi, Kibebew Kibret Tsehai, Yibekal Alemayehu Abebe and Feyera Merga Liben
Sustainability 2025, 17(15), 7077; https://doi.org/10.3390/su17157077 - 5 Aug 2025
Viewed by 114
Abstract
In the past three decades, localized research has highlighted shifts in rainfall patterns and temperature trends in central Ethiopia, a region vital for agriculture and economic activities and heavily dependent on climate conditions to sustain livelihoods and ensure food security. However, comprehensive analyses [...] Read more.
In the past three decades, localized research has highlighted shifts in rainfall patterns and temperature trends in central Ethiopia, a region vital for agriculture and economic activities and heavily dependent on climate conditions to sustain livelihoods and ensure food security. However, comprehensive analyses of long-term climate data remain limited for this area. Understanding local climate trends is essential for enhancing agricultural resilience in the study area, a region heavily dependent on rainfall for crop production. This study analyzes historical rainfall and temperature patterns over the past 30 years and projects future climate conditions using downscaled CMIP6 models under SSP4.5 and SSP8.5 scenarios. Results indicate spatial variability in rainfall trends, with certain areas showing increasing rainfall while others experience declines. Temperature has shown a consistent upward trend across all seasons, with more pronounced warming during the short rainy season (Belg). Climate projections suggest continued warming and moderate increases in annual rainfall, particularly under SSP8.5 by the end of the 21st century. It is concluded that both temperature and rainfall are projected to increase in magnitude by 2080, with higher Sen’s slope values compared to earlier periods, indicating a continued upward trend. These findings highlight potential breaks in agricultural calendars, such as shifts in rainfall onset and cessation, shortened or extended growing seasons, and increased risk of temperature-induced stress. This study highlights the need for localized adaptation strategies to safeguard agriculture production and enhance resilience in the face of future climate variability. Full article
(This article belongs to the Section Air, Climate Change and Sustainability)
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21 pages, 1141 KiB  
Article
Monthly Load Forecasting in a Region Experiencing Demand Growth: A Case Study of Texas
by Jeong-Hee Hong and Geun-Cheol Lee
Energies 2025, 18(15), 4135; https://doi.org/10.3390/en18154135 - 4 Aug 2025
Viewed by 195
Abstract
In this study, we consider monthly load forecasting, which is an essential decision for energy infrastructure planning and investment. This study focuses on the Texas power grid, where electricity consumption has surged due to rising industrial activity and the increased construction of data [...] Read more.
In this study, we consider monthly load forecasting, which is an essential decision for energy infrastructure planning and investment. This study focuses on the Texas power grid, where electricity consumption has surged due to rising industrial activity and the increased construction of data centers driven by growing demand for AI. Based on an extensive exploratory data analysis, we identify key characteristics of monthly electricity demand in Texas, including an accelerating upward trend, strong seasonality, and temperature sensitivity. In response, we propose a regression-based forecasting model that incorporates a carefully designed set of input features, including a nonlinear trend, lagged demand variables, a seasonality-adjusted month variable, average temperature of a representative area, and calendar-based proxies for industrial activity. We adopt a rolling forecasting approach, generating 12-month-ahead forecasts for both 2023 and 2024 using monthly data from 2013 onward. Comparative experiments against benchmarks including Holt–Winters, SARIMA, Prophet, RNN, LSTM, Transformer, Random Forest, LightGBM, and XGBoost show that the proposed model achieves superior performance with a mean absolute percentage error of approximately 2%. The results indicate that a well-designed regression approach can effectively outperform even the latest machine learning methods in monthly load forecasting. Full article
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26 pages, 6698 KiB  
Article
Cumulative and Lagged Effects of Drought on the Phenology of Different Vegetation Types in East Asia, 2001–2020
by Kexin Deng, Mark Henderson, Binhui Liu, Weiwei Huang, Mingyang Chen, Pingping Zheng and Ruiting Gu
Remote Sens. 2025, 17(15), 2700; https://doi.org/10.3390/rs17152700 - 4 Aug 2025
Viewed by 206
Abstract
Drought disturbances are becoming more frequent with global warming. Accurately assessing the regulatory effect of drought on vegetation phenology is key to understanding terrestrial ecosystem response mechanisms in the context of climate change. Previous studies on cumulative and lagged effects of drought on [...] Read more.
Drought disturbances are becoming more frequent with global warming. Accurately assessing the regulatory effect of drought on vegetation phenology is key to understanding terrestrial ecosystem response mechanisms in the context of climate change. Previous studies on cumulative and lagged effects of drought on vegetation growth have mostly focused on a single vegetation type or the overall vegetation NDVI, overlooking the possible influence of different adaptation strategies of different vegetation types and differences in drought effects on different phenological nodes. This study investigates the cumulative and lagged effects of drought on vegetation phenology across a region of East Asia from 2001 to 2020 using NDVI data and the Standardized Precipitation Evapotranspiration Index (SPEI). We analyzed the start of growing season (SOS) and end of growing season (EOS) responses to drought across four vegetation types: deciduous needleleaf forests (DNFs), deciduous broadleaf forests (DBFs), shrublands, and grasslands. Results reveal contrasting phenological responses: drought delayed SOS in grasslands through a “drought escape” strategy but advanced SOS in forests and shrublands. All vegetation types showed earlier EOS under drought stress. Cumulative drought effects were strongest on DNFs, SOS, and shrubland SOS, while lagged effects dominated DBFs and grassland SOS. Drought impacts varied with moisture conditions: they were stronger in dry regions for SOS but more pronounced in humid areas for EOS. By confirming that drought effects vary by vegetation type and phenology node, these findings enhance our understanding of vegetation adaptation strategies and ecosystem responses to climate stress. Full article
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21 pages, 1369 KiB  
Article
Optimizing Cold Food Supply Chains for Enhanced Food Availability Under Climate Variability
by David Hernandez-Cuellar, Krystel K. Castillo-Villar and Fernando Rey Castillo-Villar
Foods 2025, 14(15), 2725; https://doi.org/10.3390/foods14152725 - 4 Aug 2025
Viewed by 217
Abstract
Produce supply chains play a critical role in ensuring fruits and vegetables reach consumers efficiently, affordably, and at optimal freshness. In recent decades, hub-and-spoke network models have emerged as valuable tools for optimizing sustainable cold food supply chains. Traditional optimization efforts typically focus [...] Read more.
Produce supply chains play a critical role in ensuring fruits and vegetables reach consumers efficiently, affordably, and at optimal freshness. In recent decades, hub-and-spoke network models have emerged as valuable tools for optimizing sustainable cold food supply chains. Traditional optimization efforts typically focus on removing inefficiencies, minimizing lead times, refining inventory management, strengthening supplier relationships, and leveraging technological advancements for better visibility and control. However, the majority of models rely on deterministic approaches that overlook the inherent uncertainties of crop yields, which are further intensified by climate variability. Rising atmospheric CO2 concentrations, along with shifting temperature patterns and extreme weather events, have a substantial effect on crop productivity and availability. Such uncertainties can prompt distributors to seek alternative sources, increasing costs due to supply chain reconfiguration. This research introduces a stochastic hub-and-spoke network optimization model specifically designed to minimize transportation expenses by determining optimal distribution routes that explicitly account for climate variability effects on crop yields. A use case involving a cold food supply chain (CFSC) was carried out using several weather scenarios based on climate models and real soil data for California. Strawberries were selected as a representative crop, given California’s leading role in strawberry production. Simulation results show that scenarios characterized by increased rainfall during growing seasons result in increased yields, allowing distributors to reduce transportation costs by sourcing from nearby farms. Conversely, scenarios with reduced rainfall and lower yields require sourcing from more distant locations, thereby increasing transportation costs. Nonetheless, supply chain configurations may vary depending on the choice of climate models or weather prediction sources, highlighting the importance of regularly updating scenario inputs to ensure robust planning. This tool aids decision-making by planning climate-resilient supply chains, enhancing preparedness and responsiveness to future climate-related disruptions. Full article
(This article belongs to the Special Issue Climate Change and Emerging Food Safety Challenges)
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16 pages, 3226 KiB  
Article
Sustainable Agronomical Practices Affect Essential Oil Composition of Tanacetum balsamita L.
by Martina Grattacaso, Alessandra Bonetti, Sara Di Lonardo and Luigi Paolo D’Acqui
Plants 2025, 14(15), 2406; https://doi.org/10.3390/plants14152406 - 3 Aug 2025
Viewed by 279
Abstract
This study evaluated the influence of compost and bioinoculants (mycorrhizal fungi and plant growth-promoting bacteria) on the yield and composition of essential oil extracted from Tanacetum balsamita L. over two growing seasons. The plants were cultivated under four treatments: compost, bioinoculants, a combination [...] Read more.
This study evaluated the influence of compost and bioinoculants (mycorrhizal fungi and plant growth-promoting bacteria) on the yield and composition of essential oil extracted from Tanacetum balsamita L. over two growing seasons. The plants were cultivated under four treatments: compost, bioinoculants, a combination (bioinoculants + compost), and a control. At each harvest, essential oil was extracted from fresh leaves via stem-flow distillation and analyzed using gas chromatography coupled with single quadrupole mass spectrometry. Twenty to twenty-four compounds were identified. Based on the dominant terpene derivative, the results indicated that Tanacetum balsamita L. cultivated in Italy belongs to “camphor” chemotype, a pharmacologically active compound known for its antimicrobial, anti-inflammatory, and analgesic properties. Moreover, three compounds, α-, β-phellandrene and myrtenol, were identified as typical of Tanacetum balsamita L. cultivated in Italy. Treatment effects were significant for some compounds (camphor, borneol, terpinen-4-ol, α-terpineol, dehydro sabinene ketone, and 3-thujanol), and the interaction between treatment and year was significant for a few compounds (borneol, terpinen-4-ol, dehydro sabinene ketone, 1,8-cineol, and 3-thujanol). These results emphasize the need to account for seasonal variation and underline the necessity of a deeper understanding of how experimental factors interact with them, especially in long-term essential oil studies. Full article
(This article belongs to the Special Issue Chemical Analysis, Bioactivity, and Application of Essential Oils)
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20 pages, 1205 KiB  
Review
Patterns in Root Phenology of Woody Plants Across Climate Regions: Drivers, Constraints, and Ecosystem Implications
by Qiwen Guo, Boris Rewald, Hans Sandén and Douglas L. Godbold
Forests 2025, 16(8), 1257; https://doi.org/10.3390/f16081257 - 1 Aug 2025
Viewed by 186
Abstract
Root phenology significantly influences ecosystem processes yet remains poorly characterized across biomes. This study synthesized data from 59 studies spanning Arctic to tropical ecosystems to identify woody plants root phenological patterns and their environmental drivers. The analysis revealed distinct climate-specific patterns. Arctic regions [...] Read more.
Root phenology significantly influences ecosystem processes yet remains poorly characterized across biomes. This study synthesized data from 59 studies spanning Arctic to tropical ecosystems to identify woody plants root phenological patterns and their environmental drivers. The analysis revealed distinct climate-specific patterns. Arctic regions had a short growing season with remarkably low temperature threshold for initiation of root growth (0.5–1 °C). Temperate forests displayed pronounced spring-summer growth patterns with root growth initiation occurring at 1–9 °C. Mediterranean ecosystems showed bimodal patterns optimized around moisture availability, and tropical regions demonstrate seasonality primarily driven by precipitation. Root-shoot coordination varies predictably across biomes, with humid continental ecosystems showing the highest synchronous above- and belowground activity (57%), temperate regions exhibiting leaf-before-root emergence (55%), and Mediterranean regions consistently showing root-before-leaf patterns (100%). Winter root growth is more widespread than previously recognized (35% of studies), primarily in tropical and Mediterranean regions. Temperature thresholds for phenological transitions vary with climate region, suggesting adaptations to environmental conditions. These findings provide a critical, region-specific framework for improving models of terrestrial ecosystem responses to climate change. While our synthesis clarifies distinct phenological strategies, its conclusions are drawn from data focused primarily on Northern Hemisphere woody plants, highlighting significant geographic gaps in our current understanding. Bridging these knowledge gaps is essential for accurately forecasting how belowground dynamics will influence global carbon sequestration, nutrient cycling, and ecosystem resilience under changing climatic regimes. Full article
(This article belongs to the Section Forest Ecophysiology and Biology)
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24 pages, 3888 KiB  
Article
Agronomic Biofortification: Enhancing the Grain Nutritional Composition and Mineral Content of Winter Barley (Hordeum vulgare L.) Through Foliar Nutrient Application Under Different Soil Tillage Methods
by Amare Assefa Bogale, Zoltan Kende, István Balla, Péter Mikó, Boglárka Bozóki and Attila Percze
Agriculture 2025, 15(15), 1668; https://doi.org/10.3390/agriculture15151668 - 1 Aug 2025
Viewed by 234
Abstract
Enhancing the nutritional content of crops is crucial for safeguarding human health and mitigating global hunger. A viable method for attaining this goal is the planned implementation of various agronomic practices, including tillage and nutrient provision. A field experiment was executed at the [...] Read more.
Enhancing the nutritional content of crops is crucial for safeguarding human health and mitigating global hunger. A viable method for attaining this goal is the planned implementation of various agronomic practices, including tillage and nutrient provision. A field experiment was executed at the Hungarian University of Agriculture and Life Sciences in Gödöllő in the 2023 and 2024 growing seasons. The study aimed to assess the effects of foliar nutrient supply and soil tillage methods on the grain nutritional composition and mineral content of winter barley. Employing a split-plot design with three replications, the experiment included four nutrient treatments (control, bio-cereal, bio-algae, and MgSMnZn blend) and two soil tillage types (i.e., plowing and cultivator). The results indicated that while protein content was not influenced by the main effects of nutrients and tillage, the levels of β-glucan, starch, crude ash, and moisture content were significantly (p < 0.05) affected by the nutrient treatments and by growing year, treated as a random factor. Notably, bio-algae and bio-cereal nutrients, combined with cultivator tillage, enhanced β-glucan content. All applied nutrient treatments increased the level of starch compared to the control. With regard to grain mineral content, the iron and zinc content responded to the nutrient supply, tillage, and growing year. However, applying a multiple-nutrient composition-based treatment did not increase iron and zinc levels, suggesting that individual applications may be more effective for increasing the content of these minerals in grains. Cultivator tillage improved iron and zinc levels. Moreover, manganese (Mn) and copper (Cu) were predominantly affected by nutrient availability and by growing seasons as a random factor. Therefore, to improve grain quality, this study emphasizes the significance of proper nutrient and tillage methods by focusing on the intricate relationships between agronomic techniques and environmental factors that shape barley’s nutritional profile. Full article
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21 pages, 23129 KiB  
Article
Validation of Global Moderate-Resolution FAPAR Products over Boreal Forests in North America Using Harmonized Landsat and Sentinel-2 Data
by Yinghui Zhang, Hongliang Fang, Zhongwen Hu, Yao Wang, Sijia Li and Guofeng Wu
Remote Sens. 2025, 17(15), 2658; https://doi.org/10.3390/rs17152658 - 1 Aug 2025
Viewed by 129
Abstract
The fraction of absorbed photosynthetically active radiation (FAPAR) stands as a pivotal parameter within the Earth system, quantifying the energy exchange between vegetation and solar radiation. Accordingly, there is an urgent need for comprehensive validation studies to accurately quantify uncertainties and improve the [...] Read more.
The fraction of absorbed photosynthetically active radiation (FAPAR) stands as a pivotal parameter within the Earth system, quantifying the energy exchange between vegetation and solar radiation. Accordingly, there is an urgent need for comprehensive validation studies to accurately quantify uncertainties and improve the reliability of FAPAR-based applications. This study validated five global FAPAR products, MOD15A2H, MYD15A2H, VNP15A2H, GEOV2, and GEOV3, over four boreal forest sites in North America. Qualitative quality flags (QQFs) and quantitative quality indicators (QQIs) of each product were analyzed. Time series high-resolution reference FAPAR maps were developed using the Harmonized Landsat and Sentinel-2 dataset. The reference FAPAR maps revealed a strong agreement with the in situ FAPAR from AmeriFlux (correlation coefficient (R) = 0.91; root mean square error (RMSE) = 0.06). The results revealed that global FAPAR products show similar uncertainties (RMSE: 0.16 ± 0.04) and moderate agreement with the reference FAPAR (R = 0.75 ± 0.10). On average, 34.47 ± 6.91% of the FAPAR data met the goal requirements of the Global Climate Observing System (GCOS), while 54.41 ± 6.89% met the threshold requirements of the GCOS. Deciduous forests perform better than evergreen forests, and the products tend to underestimate the reference data, especially for the beginning and end of growing seasons in evergreen forests. There are no obvious quality differences at different QQFs, and the relative QQI can be used to filter high-quality values. To enhance the regional applicability of global FAPAR products, further algorithm improvements and expanded validation efforts are essential. Full article
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19 pages, 3489 KiB  
Article
Impact of Nitrogen Fertilisation and Inoculation on Soybean Nodulation, Nitrogen Status, and Yield in a Central European Climate
by Waldemar Helios, Magdalena Serafin-Andrzejewska, Marcin Kozak and Sylwia Lewandowska
Agriculture 2025, 15(15), 1654; https://doi.org/10.3390/agriculture15151654 - 1 Aug 2025
Viewed by 215
Abstract
Soybean (Glycine max [L.] Merr.) cultivation is expanding in Central Europe due to the development of early-maturing cultivars and growing demand for plant-based protein produced without the use of genetically modified organisms. However, nitrogen (N) management remains a major challenge in temperate [...] Read more.
Soybean (Glycine max [L.] Merr.) cultivation is expanding in Central Europe due to the development of early-maturing cultivars and growing demand for plant-based protein produced without the use of genetically modified organisms. However, nitrogen (N) management remains a major challenge in temperate climates, where variable weather conditions can significantly affect nodulation and yield. This study evaluated the effects of three nitrogen fertilisation doses (0, 30, and 60 kg N·ha−1), applied in the form of ammonium nitrate (34% N) and two commercial rhizobial inoculants—HiStick Soy (containing Bradyrhizobium japonicum strain 532C) and Nitragina (including a Polish strain of B. japonicum)—on nodulation, nitrogen uptake, and seed yield. A three-year field experiment (2017–2019) was conducted in southwestern Poland using a two-factor randomized complete block design. Nodulation varied significantly across years, with the highest values recorded under favourable early-season moisture and reduced during drought. In the first year, inoculation with HiStick Soy significantly increased nodule number and seed yield compared to Nitragina and the uninoculated control. Nitrogen fertilisation consistently improved seed yield, although it had no significant effect on nodulation. The highest nitrogen use efficiency was observed with moderate nitrogen input (30 kg N·ha−1) combined with inoculation. These findings highlight the importance of integrating effective rhizobial inoculants with optimized nitrogen fertilisation to improve soybean productivity and nitrogen efficiency under variable temperate climate conditions. Full article
(This article belongs to the Special Issue Strategies to Enhance Nutrient Use Efficiency and Crop Nutrition)
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10 pages, 787 KiB  
Article
Association of Temperament with Growth Performance in Nili Ravi Buffalo Heifers
by Salman Khalid Gorsi, Hamza Manzoor and Muhammad Qamer Shahid
Animals 2025, 15(15), 2255; https://doi.org/10.3390/ani15152255 - 31 Jul 2025
Viewed by 206
Abstract
This study investigated the association between temperament and retrospective growth rates in 84 Nili Ravi buffalo heifers aged 18 to 24 months. Temperament was assessed using chute score and exit velocity, measured twice at a seven-day interval, and classified as calm (≤3) or [...] Read more.
This study investigated the association between temperament and retrospective growth rates in 84 Nili Ravi buffalo heifers aged 18 to 24 months. Temperament was assessed using chute score and exit velocity, measured twice at a seven-day interval, and classified as calm (≤3) or nervous (>3). Retrospective average daily weight gain data were retrieved from farm records, and blood samples were collected to measure cortisol levels. ANOVA was used to analyze data, considering temperament, age group, season, and year of birth as fixed effects, with birth weight as a covariate. Results showed that 48 heifers were calm and 36 were nervous. Calm heifers exhibited significantly higher average daily gains than nervous heifers during the post-weaning period, with an increase of 240 g/day from 4 to 6 months and 190 g/day from 6 to 12 months (p < 0.001). However, this difference was not significant at 18–24 months (p = 0.144). Calm heifers have numerically lower cortisol levels (0.96 vs. 1.27 μg/dL; p = 0.11). These findings suggest that calmer heifers grow faster in early life, emphasizing the importance of temperament in breeding programs aimed at improving growth performance and welfare. Full article
(This article belongs to the Special Issue Buffalo Farming as a Tool for Sustainability)
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20 pages, 4135 KiB  
Article
Climate-Induced Water Management Challenges for Cabbage and Carrot in Southern Poland
by Stanisław Rolbiecki, Barbara Jagosz, Roman Rolbiecki and Renata Kuśmierek-Tomaszewska
Sustainability 2025, 17(15), 6975; https://doi.org/10.3390/su17156975 - 31 Jul 2025
Viewed by 267
Abstract
Climate warming poses significant challenges for the sustainable management of natural water resources, making efficient planning and usage essential. This study evaluates the water requirements, irrigation demand, and rainfall deficits for two key vegetable crops, carrot and white cabbage, under projected climate scenarios [...] Read more.
Climate warming poses significant challenges for the sustainable management of natural water resources, making efficient planning and usage essential. This study evaluates the water requirements, irrigation demand, and rainfall deficits for two key vegetable crops, carrot and white cabbage, under projected climate scenarios RCP 4.5 and RCP 8.5 for the period 2031–2100. The analysis was conducted for Kraków and Rzeszów Counties in southern Poland using projected monthly temperature and precipitation data from the Klimada 2.0 portal. Potential evapotranspiration (ETp) during the growing season (May–October) was estimated using Treder’s empirical model and the crop coefficient method adapted for Polish conditions. The reference period for comparison was 1951–2020. The results reveal a significant upward trend in water demand for both crops, with the highest increases under the RCP 8.5 scenario–seasonal ETp values reaching up to 517 mm for cabbage and 497 mm for carrot. Rainfall deficits are projected to intensify, especially during July and August, with greater shortages in Rzeszów County compared to Kraków County. Irrigation demand varies depending on soil type and drought severity, becoming critical in medium and very dry years. These findings underscore the necessity of adapting irrigation strategies and water resource management to ensure sustainable vegetable production under changing climate conditions. The data provide valuable guidance for farmers, advisors, and policymakers in planning effective irrigation infrastructure and optimizing water-use efficiency in southern Poland. Full article
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16 pages, 494 KiB  
Article
Comparative Analysis of Yield and Grain-Filling Characteristics of Conventional Rice with Different Panicle Types in Response to Nitrogen Fertilization
by Nianbing Zhou, Tong Sun, Yanhong Zhang, Qiang Shi, Yu Zhou, Qiangqiang Xiong, Jinlong Hu, Shuai Wang and Jinyan Zhu
Agronomy 2025, 15(8), 1858; https://doi.org/10.3390/agronomy15081858 - 31 Jul 2025
Viewed by 239
Abstract
This study investigated the impact of nitrogen (N) fertilization on the yield and grain filling (GF) characteristics of two conventional japonica rice varieties with distinct panicle types: Yangchan 3501 (large-panicle: spikelets per panicle > 150) and Nangeng 46 (medium-panicle: [...] Read more.
This study investigated the impact of nitrogen (N) fertilization on the yield and grain filling (GF) characteristics of two conventional japonica rice varieties with distinct panicle types: Yangchan 3501 (large-panicle: spikelets per panicle > 150) and Nangeng 46 (medium-panicle: 100 < spikelets per panicle < 150). Field experiments were conducted over two growing seasons (2022–2023) with three N application rates (T1: 225 kg ha−1, T2: 270 kg ha−1, T3: 315 kg ha−1). Key measurements included tiller dynamics, panicle composition, GF parameters modeled using the Richards equation, and enzyme activities related to nitrogen metabolism (Fd-GOGAT, NR) and carbohydrate transport (α-amylase, SPS). Results showed that the yield increased with higher N levels for both varieties, with Yangchan 3501 achieving higher yields primarily through increased grains per panicle (15.65% rise under T3 vs. T1), while Nangeng 46 relied on panicle number (8.83% increase under T3 vs. T1). Nitrogen application enhanced Fd-GOGAT and NR activities, prolonging photosynthesis and improving GF rates, particularly in the inferior grains of Yangchan 3501 during middle and late stages. However, a high N reduced seed-setting rates and 1000-grain weight, with larger panicle types exhibiting a greater sensitivity to N-induced changes in branch structure and assimilate allocation. This study highlights that optimizing N management can improve nitrogen-metabolism enzyme activity and GF efficiency, especially in large-panicle rice, while medium-panicle types require higher N inputs to maximize panicle number. These findings provide actionable insights for achieving high yields and efficient nutrient use in conventional rice cultivation. Full article
(This article belongs to the Section Soil and Plant Nutrition)
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24 pages, 1117 KiB  
Article
Comparative Analysis of Pesticide Residues in Hive Products from Rapeseed (Brassica napus subsp. napus) and Sunflower (Helianthus annuus) Crops Under Varying Agricultural Practices in Romania During the 2020–2021 Beekeeping Seasons
by Dan Bodescu, Viorel Fătu, Agripina Şapcaliu, Elena Luiza Bădic, Roxana Zaharia, Dana Tăpăloagă, Alexandru-Dragoș Robu and Radu-Adrian Moraru
Agriculture 2025, 15(15), 1648; https://doi.org/10.3390/agriculture15151648 - 31 Jul 2025
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Abstract
Over the past years, increasing attention has been drawn to the adverse effects of agricultural pesticide use on pollinators, with honeybees being especially vulnerable. The aim of this study was to evaluate the levels of residues detectable and/or quantifiable of neonicotinoid pesticides and [...] Read more.
Over the past years, increasing attention has been drawn to the adverse effects of agricultural pesticide use on pollinators, with honeybees being especially vulnerable. The aim of this study was to evaluate the levels of residues detectable and/or quantifiable of neonicotinoid pesticides and other pesticides in biological materials (bees, bee brood, etc.) and beehive products (honey, pollen, etc.) applied as seed dressings in rapeseed and sunflower plants in two growing seasons (2020–2021) in fields located in three agro-climatic regions in Romania. The study involved the comparative sampling of hive products (honey, pollen, adult bees, and brood) from experimental and control apiaries, followed by pesticide residue analysis in an accredited laboratory (Primoris) using validated chromatographic techniques (LC-MS/MS and GC-MS). Toxicological analyses of 96 samples, including bees, bee brood, honey, and pollen, confirmed the presence of residues in 46 samples, including 10 bee samples, 10 bee brood samples, 18 honey samples, and 8 pollen bread samples. The mean pesticide residue concentrations detected in hive products were 0.032 mg/kg in honey, 0.061 mg/kg in pollen, 0.167 mg/kg in bees, and 0.371 mg/kg in bee brood. The results highlight the exposure of honeybee colonies to multiple sources of pesticide residue contamination, under conditions where legal recommendations for the controlled application of agricultural treatments are not followed. The study provides relevant evidence for strengthening the risk assessment framework and underscores the need for adopting stricter monitoring and regulatory measures to ensure the protection of honeybee colony health. Full article
(This article belongs to the Section Agricultural Product Quality and Safety)
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